G M Lacourciere

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Selenium is mobilized in vivo from free selenocysteine and is incorporated specifically into formate dehydrogenase H and tRNA nucleosides
    Gerard M Lacourciere
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 184:1940-6. 2002
  2. ncbi request reprint Utilization of selenocysteine as a source of selenium for selenophosphate biosynthesis
    G M Lacourciere
    Laboratory of Biochemistry, National Institutes of Health, NHLBI, 50 South Drive, Room 2126, Bethesda, MD 20892, USA
    Biofactors 14:69-74. 2001
  3. ncbi request reprint Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate
    G M Lacourciere
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:23769-73. 2000
  4. ncbi request reprint Biosynthesis of selenophosphate
    G M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biofactors 10:237-44. 1999
  5. pmc Catalytic properties of selenophosphate synthetases: comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli
    G M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 96:44-8. 1999
  6. ncbi request reprint Responsiveness of selenoproteins to dietary selenium
    C B Allan
    NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Nutr 19:1-16. 1999
  7. pmc The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine-containing formate dehydrogenase H
    Hisaaki Mihara
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Proc Natl Acad Sci U S A 99:6679-83. 2002
  8. ncbi request reprint Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase
    Matt D Wolfe
    Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    J Biol Chem 279:1801-9. 2004
  9. pmc Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins
    Gerard M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:9150-3. 2002
  10. pmc Characterization of potential selenium-binding proteins in the selenophosphate synthetase system
    Yuki Ogasawara
    Department of Environmental Biology, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Nishitokyo, Tokyo 204 8588, Japan
    Proc Natl Acad Sci U S A 102:1012-6. 2005

Collaborators

Detail Information

Publications10

  1. pmc Selenium is mobilized in vivo from free selenocysteine and is incorporated specifically into formate dehydrogenase H and tRNA nucleosides
    Gerard M Lacourciere
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 184:1940-6. 2002
    ..The ability of E. coli to utilize selenocysteine as a selenium source for selenophosphate biosynthesis in vivo supports the participation of the NifS-like proteins in selenium metabolism...
  2. ncbi request reprint Utilization of selenocysteine as a source of selenium for selenophosphate biosynthesis
    G M Lacourciere
    Laboratory of Biochemistry, National Institutes of Health, NHLBI, 50 South Drive, Room 2126, Bethesda, MD 20892, USA
    Biofactors 14:69-74. 2001
    ..All three proteins exhibit lyase activity on L-cysteine and L-selenocysteine and produce sulfane sulfur, S(0), or Se(0) respectively. Each lyase can effectively mobilize Se(0) from L-selenocysteine for selenophosphate biosynthesis...
  3. ncbi request reprint Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate
    G M Lacourciere
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:23769-73. 2000
    ..Taken together the ability of the NifS-like proteins to generate a selenium substrate for SPS and the activation of the SPS (C17S) mutant suggest a selenium delivery function for the proteins in vivo...
  4. ncbi request reprint Biosynthesis of selenophosphate
    G M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biofactors 10:237-44. 1999
    ..The inactive purified human enzyme together with the very low determined specific activity of the E. coli enzyme (83 nmol/min/mg) suggest an essential component for the formation of selenophosphate has not been identified...
  5. pmc Catalytic properties of selenophosphate synthetases: comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli
    G M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 96:44-8. 1999
    ..However, the selenocysteine-containing enzyme did not exhibit the expected higher catalytic activity. Based on these results we suggest a role of selenocysteine in H. influenzae that is not catalytic...
  6. ncbi request reprint Responsiveness of selenoproteins to dietary selenium
    C B Allan
    NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Nutr 19:1-16. 1999
    ..In general, selenoprotein levels in brain are less sensitive to dietary selenium fluctuation than the corresponding selenoprotein levels in other tissues...
  7. pmc The iscS gene is essential for the biosynthesis of 2-selenouridine in tRNA and the selenocysteine-containing formate dehydrogenase H
    Hisaaki Mihara
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Proc Natl Acad Sci U S A 99:6679-83. 2002
    ..The lack of IscS also caused a significant loss of the selenium-containing polypeptide of formate dehydrogenase H. Together, these results suggest a dual function of IscS in sulfur and selenium metabolism...
  8. ncbi request reprint Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase
    Matt D Wolfe
    Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8012, USA
    J Biol Chem 279:1801-9. 2004
    ..These data are consistent with the ybbB gene encoding a tRNA 2-selenouridine synthase and identifies a new role for the rhodanese homology domain in enzymes...
  9. pmc Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins
    Gerard M Lacourciere
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:9150-3. 2002
    ..A 28-kDa protein identified as deoxyribose phosphate aldolase also contained bound selenium. These (75)Se-labeled proteins may have alternate roles as selenium delivery proteins...
  10. pmc Characterization of potential selenium-binding proteins in the selenophosphate synthetase system
    Yuki Ogasawara
    Department of Environmental Biology, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Nishitokyo, Tokyo 204 8588, Japan
    Proc Natl Acad Sci U S A 102:1012-6. 2005
    ..In the presence of a selenium-binding protein, a low level of selenodiglutathione formed from SeO3(2-) and glutathione could effectively replace the high concentrations of selenide routinely used as substrate in the SPS in vitro assays...