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Genomes and Genes | Richard A KoupSummaryAffiliation: National Institutes of Health Country: USA Publications
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Vaccine design for CD8 T lymphocyte responsesRichard A Koup
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cold Spring Harb Perspect Med 1:a007252. 2011....- Standardization of cytokine flow cytometry assaysHolden T Maecker
BD Biosciences, San Jose, USA
BMC Immunol 6:13. 2005.... - Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trialsRichard A Koup
Immunology Laboratory, Immunology Core Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 83:6318-22. 2009..These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4(+) T cells... - Reconsidering early HIV treatment and supervised treatment interruptionsRichard A Koup
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
PLoS Med 1:e41. 2004 - Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 DNA candidate vaccineBarney S Graham
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3017, USA
J Infect Dis 194:1650-60. 2006..Vaccine Research Center (VRC) 004 is the first phase 1 dose-escalation study of a multiclade HIV-1 DNA vaccine... - Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturationJoseph P Casazza
Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID, National Institutes of Health (NIH, Bethesda, MD 20892, USA
J Exp Med 203:2865-77. 2006..Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes... 
The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentrationMichael R Betts
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 172:6407-17. 2004..The inherent ability of viruses to induce high or low Ag states may be the primary determinant of the cytokine vs cytolytic nature of the virus-specific CD8(+) T cell response...- Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vectorAndrew T Catanzaro
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3017, USA
J Infect Dis 194:1638-49. 2006..This multiclade rAd5 HIV-1 vaccine is now being evaluated in combination with a multiclade HIV-1 DNA plasmid vaccine... - Priming immunization with DNA augments immunogenicity of recombinant adenoviral vectors for both HIV-1 specific antibody and T-cell responsesRichard A Koup
Vaccine Research Center, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 5:e9015. 2010..Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses in preclinical studies... - Phase I clinical evaluation of a six-plasmid multiclade HIV-1 DNA candidate vaccineAndrew T Catanzaro
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Building 40, Bethesda, MD 20892 3017, USA
Vaccine 25:4085-92. 2007..Compared to a four-plasmid product, Gag- and Nef-specific T cell responses were improved, while Env-specific responses were maintained. This candidate vaccine has now advanced to Phase II evaluation... - T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesisJason M Brenchley
Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 78:1160-8. 2004..These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection... - SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, 40 Convent Drive, Bethesda, MD 20892, USA
Blood 110:928-36. 2007..Manipulation of the interaction of PD-1 with its ligands could thus potentially restore the CD8+ T-cell responses in SIV infection... - Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
J Immunol 183:1120-32. 2009..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection... - Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primatesUlrike Wille-Reece
Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 203:1249-58. 2006..These data provide insights for designing prime-boost immunization regimens to optimize Th1 and CD8+ T cell responses... - Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responsesKarin Lore
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 179:1721-9. 2007..Thus, the ability of rAd35 to naturally target important DC subsets, induce their maturation, and appropriately present Ag to T cells may herald greater in vivo immunogenicity than has been observed with rAd5... - Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA virusesDavid A Price
Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 202:1349-61. 2005..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function... - Biodistribution and toxicological safety of adenovirus type 5 and type 35 vectored vaccines against human immunodeficiency virus-1 (HIV-1), Ebola, or Marburg are similar despite differing adenovirus serotype vector, manufacturer's construct, or gene inserRebecca L Sheets
Vaccine Research Center, NIH NIAID, Bethesda, Maryland 20892 7628, USA
J Immunotoxicol 5:315-35. 2008..These data demonstrate the safety and suitability for investigational human use of Ad5 or Ad35 adenovector-based vaccine candidates at doses of up to 2 x 10(11) given intramuscularly to prevent various infectious diseases... - A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trialJulie E Martin
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, MSC 2610, Bethesda, MD 20892 3017, USA
Vaccine 26:6338-43. 2008..Over 8000 cases and 900 deaths occurred during the epidemic. We report the safety and immunogenicity of a SARS DNA vaccine in a Phase I human study... - Immune activation driven by CTLA-4 blockade augments viral replication at mucosal sites in simian immunodeficiency virus infectionValentina Cecchinato
Animal Models and Retroviral Vaccines Section, National Cancer Institute, Bethesda, MD 20892 5065, USA
J Immunol 180:5439-47. 2008..These data provide the first direct evidence that immune activation drives viral replication, and suggest caution in the use of therapeutic approaches for HIV infection in vivo that increase CD4(+) T cell proliferation... - Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responsesMelissa L Precopio
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 204:1405-16. 2007..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated... - HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cellsMichael R Betts
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Blood 107:4781-9. 2006..Thus, rather than quantity or phenotype, the quality of the CD8(+) T-cell functional response serves as an immune correlate of HIV disease progression and a potential qualifying factor for evaluation of HIV vaccine efficacy... - Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infectionMichael R Betts
Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:4512-7. 2005..These data suggest that control of HIV by vaccine-elicited HIV-specific T cell responses may be difficult, even when the T cell response has those characteristics predicted to provide optimal protection... - Preferential infection and depletion of Mycobacterium tuberculosis-specific CD4 T cells after HIV-1 infectionChristof Geldmacher
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 207:2869-81. 2010.... - Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infectionJoseph P Casazza
Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
PLoS Pathog 5:e1000646. 2009..These data suggest that CD4+ T cells which produce MIP-1alpha and MIP-1beta bind these chemokines in an autocrine fashion which decreases the risk of in vivo HIV infection... - Amine-reactive dyes for dead cell discrimination in fixed samplesStephen P Perfetto
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
Curr Protoc Cytom . 2010..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue... - HIV Gag protein conjugated to a Toll-like receptor 7/8 agonist improves the magnitude and quality of Th1 and CD8+ T cell responses in nonhuman primatesUlrike Wille-Reece
Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:15190-4. 2005..This type of vaccine formulation should have utility in preventive or therapeutic vaccines in which humoral and cellular immunity is required... - Minor viral and host genetic polymorphisms can dramatically impact the biologic outcome of an epitope-specific CD8 T-cell responseChristof Geldmacher
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA
Blood 114:1553-62. 2009..Thus, minor viral and host genetic polymorphisms can dramatically alter the immunologic and virologic outcome of an epitope-specific CD8 T-cell response... - Safety and immunogenicity of a Gag-Pol candidate HIV-1 DNA vaccine administered by a needle-free device in HIV-1-seronegative subjectsJorge A Tavel
Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Acquir Immune Defic Syndr 44:601-5. 2007..To evaluate the safety and immunogenicity of a candidate HIV DNA vaccine administered using a needle-free device... - Interleukin-15 but not interleukin-7 abrogates vaccine-induced decrease in virus level in simian immunodeficiency virus mac251-infected macaquesAnna Hryniewicz
Animal Models and Retroviral Vaccines Section, National Cancer Institute, Building 41, Bethesda, MD 20892, USA
J Immunol 178:3492-504. 2007..These results underscore the importance of testing immunomodulatory approaches in vivo to assess potential risks and benefits for HIV-1-infected individuals... - Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPsNancy J Sullivan
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
PLoS Med 3:e177. 2006..GP can exert cytopathic effects on transfected cells in vitro, and multiple GP forms have been identified in nature, raising the question of which would be optimal for a human vaccine... - Differential specificity and immunogenicity of adenovirus type 5 neutralizing antibodies elicited by natural infection or immunizationCheng Cheng
Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD 20892 3005, USA
J Virol 84:630-8. 2010..These results have implications for future AIDS vaccine trials and the design of next-generation gene-based vaccine vectors... - Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cellsJason M Brenchley
Vaccine Research Center and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Blood 101:2711-20. 2003..Thus, our studies define a phenotype associated with replicative senescence in HIV-specific CD8(+) T cells, which may have broad implications to other conditions associated with chronic antigenic stimulation... - Type I IFN induced by adenovirus serotypes 28 and 35 has multiple effects on T cell immunogenicityMatthew J Johnson
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 188:6109-18. 2012..Taken together, our results demonstrate that rAd-induced IFN-α production has multiple effects on T cell immunogenicity, the understanding of which should be considered in the design of rAd vaccine vectors... - Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infectionTakuya Yamamoto
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
J Virol 86:5877-84. 2012..The ability to elicit such virus-specific EM CD8(+) T cells might contribute substantially to an efficacious HIV/AIDS vaccine, even after breakthrough infection... - Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometryPratip K Chattopadhyay
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Med 12:972-7. 2006.... - Preferential infection shortens the life span of human immunodeficiency virus-specific CD4+ T cells in vivoJason M Brenchley
Human Virology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
J Virol 80:6801-9. 2006.... - HIV preferentially infects HIV-specific CD4+ T cellsDaniel C Douek
Vaccine Research Center, NIAID, NIH, Maryland 20892, USA
Nature 417:95-8. 2002..Furthermore, the phenomenon of HIV specifically infecting the very cells that respond to it adds a cautionary note to the practice of structured therapy interruption... - Immunologic pressure within class I-restricted cognate human immunodeficiency virus epitopes during highly active antiretroviral therapyJoseph P Casazza
Immunology Laboratory, National Institutes of Health, Bethesda, MD 20892, USA
J Virol 79:3653-63. 2005.... - Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infectionTakuya Yamamoto
National Institutes of Health, Bethesda, MD, USA
Blood 117:4805-15. 2011..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons... - T cell receptor recognition motifs govern immune escape patterns in acute SIV infectionDavid A Price
Human Immunology Section, Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA
Immunity 21:793-803. 2004..These findings have profound implications for the development of vaccines that elicit T cell immunity to combat pathogens with unstable genomes... - PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 203:2281-92. 2006.... 
CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primatesNancy J Sullivan
Vaccine Research Center, US National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA
Nat Med 17:1128-31. 2011..Understanding the immunologic mechanism of Ebola virus protection will facilitate the development of vaccines for Ebola and related hemorrhagic fever viruses in humans...- Keratinocyte growth factor augments immune reconstitution after autologous hematopoietic progenitor cell transplantation in rhesus macaquesRuth Seggewiss
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
Blood 110:441-9. 2007..Thus, our findings suggest that KGF may be a useful adjuvant therapy to augment T-cell reconstitution after human PBPC transplantation... - Myeloid and plasmacytoid dendritic cells transfer HIV-1 preferentially to antigen-specific CD4+ T cellsKarin Lore
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 201:2023-33. 2005..This suggests that the induction of antigen-specific T cell responses by DCs, a process crucial to immune defense, can lead to preferential HIV-1 infection and the deletion of responding CD4(+) T cells... - CD4 T follicular helper cell dynamics during SIV infectionConstantinos Petrovas
Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
J Clin Invest 122:3281-94. 2012..Therefore, chronic SIV does not disturb the ability of TFH cells to help B cell maturation and production of SIV-specific immunoglobulins... - Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus SpeciesLisa E Hensley
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
PLoS Pathog 6:e1000904. 2010.... - Early depletion of Mycobacterium tuberculosis-specific T helper 1 cell responses after HIV-1 infectionChristof Geldmacher
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Infect Dis 198:1590-8. 2008..This study was conducted to better understand the mechanism underlying M. tuberculosis-specific pathogenicity early after onset of HIV infection... - HIV-infected Langerhans cells preferentially transmit virus to proliferating autologous CD4+ memory T cells located within Langerhans cell-T cell clustersMakoto Sugaya
Dermatology Branch, Center for Cancer Research, National Cancer Institute, and Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
J Immunol 172:2219-24. 2004..Disrupting cluster formation between LC and memory CD4(+) T cells may be a novel strategy to interfere with sexual transmission of HIV... - Safety, immunogenicity and efficacy of modified vaccinia Ankara (MVA) against Dryvax challenge in vaccinia-naïve and vaccinia-immune individualsJanie Parrino
Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Vaccine 25:1513-25. 2007..MVA vaccination is safe and immunogenic and improves the safety and immunogenicity of subsequent Dryvax vaccination supporting the potential for using MVA as a vaccine in the general population to improve immunity to orthopoxviruses... - In vitro culture during retroviral transduction improves thymic repopulation and output after total body irradiation and autologous peripheral blood progenitor cell transplantation in rhesus macaquesKarin Lore
Immunology Laboratory, Research Center, National Institute of Allergy and Infectious Diseases, NIH, Department of Health and Human Services, Bethesda, Maryland 20892 1202, USA
Stem Cells 24:1539-48. 2006..These results have implications for the design of gene therapy trials, as well as for the use of expanded PBPCs for improved T-cell immune reconstitution after transplantation... - Viable infectious cell sorting in a BSL-3 facilityStephen P Perfetto
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 263:419-24. 2004..With this system in place, aerosol containment can be measured quickly and efficiently, therefore reducing the risk to the operator when sorting viable infectious cells... - A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trialJulie E Martin
Vaccine Research Center, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
J Infect Dis 196:1732-40. 2007..West Nile virus (WNV) is a mosquito-borne flavivirus that can cause severe meningitis and encephalitis in infected individuals. We report the safety and immunogenicity of a WNV DNA vaccine in its first phase 1 human study... - Emerging concepts in the immunopathogenesis of AIDSDaniel C Douek
Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Med 60:471-84. 2009.... - Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulationMichael R Betts
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
J Immunol Methods 281:65-78. 2003.... - A DNA vaccine for Ebola virus is safe and immunogenic in a phase I clinical trialJulie E Martin
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes, Bethesda, MD 20892 3017, USA
Clin Vaccine Immunol 13:1267-77. 2006.... - T cell dynamics in HIV-1 infectionDaniel C Douek
Human Immunology Section Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
Annu Rev Immunol 21:265-304. 2003.... - A West Nile virus DNA vaccine utilizing a modified promoter induces neutralizing antibody in younger and older healthy adults in a phase I clinical trialJulie E Ledgerwood
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Infect Dis 203:1396-404. 2011..There are no licensed vaccines to prevent WNV in humans. The safety and immunogenicity of a first-generation WNV DNA vaccine was demonstrated in a clinical trial and a similar DNA vaccine has been licensed for use in horses... - Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responsesKarin Lore
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3022, USA
J Immunol 171:4320-8. 2003.... - Optimizing peptide matrices for identifying T-cell antigensMelissa L Precopio
Immunology Laboratory, Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
Cytometry A 73:1071-8. 2008..This optimized deconvolution method allows for efficient mapping of T-cell peptide epitopes. It is rapid, powerful, efficient, and unrestricted by HLA type... - Standard practice for cell sorting in a BSL-3 facilityStephen P Perfetto
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 699:449-69. 2011..Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation... - Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primatesNancy J Sullivan
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892-3005, USA
Nature 424:681-4. 2003..This accelerated vaccine provides an intervention that may help to limit the epidemic spread of Ebola, and is applicable to other viruses... - Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometryStephen P Perfetto
Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
J Immunol Methods 313:199-208. 2006..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments... - Detection of T-cell degranulation: CD107a and bMichael R Betts
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Methods Cell Biol 75:497-512. 2004 - Optimal antigens for HIV vaccines based on CD8+ T response, protein length, and sequence variabilityMichael R Betts
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
DNA Cell Biol 21:665-70. 2002..The accessory proteins Tat, Rev, Vif, Vpr, and Vpu in general all elicit very low CD8+ T cell responses, and this, in combination with their high variability, makes them less attractive as vaccine antigen... - IL-7, the thymus, and naïve T cellsYukari Okamoto
Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
Adv Exp Med Biol 512:81-90. 2002 - Heavy metal contaminants can eliminate quantum dot fluorescenceDavid Zarkowsky
Laboratory of Immunology, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland USA
Cytometry A 79:84-9. 2011..Indeed, these cells can then be successfully stained with other QD reagents, providing a method for immunofluorescence restaining of cells without contaminating fluorescence from the first stain... - The quest for a T cell-based immune correlate of protection against HIV: a story of trials and errorsRichard A Koup
Richard A Koup, Barney S Graham and Daniel C Douek are at the Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 3017, USA
Nat Rev Immunol 11:65-70. 2011..Our view is that investing in an iterative approach to human vaccine efficacy trials of sufficient size and sampling frequency will improve the likelihood that an immune correlate of vaccine protection will be defined... - Infection of specific dendritic cells by CCR5-tropic human immunodeficiency virus type 1 promotes cell-mediated transmission of virus resistant to broadly neutralizing antibodiesLakshmanan Ganesh
Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bldg 40, Rm 4502, MSC 3005, 40 Convent Dr, Bethesda, MD 20892 3005, USA
J Virol 78:11980-7. 2004..This property of DCs may enhance infection, contribute to immune evasion, and could provide a selective advantage for CCR5-tropic virus transmission... - A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escapeDaniel C Douek
Department of Experimental Transplantation and Immunology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 168:3099-104. 2002..Thus, CD8(+) T cells comprising multiple TCR clonotypes may expand in vivo in response to individual epitopes, and may increase the ability of the response to recognize virus escape mutants... - Optimized determination of T cell epitope responsesMario Roederer
Vaccine Research Center, NIAID, NIH, 40 Convent Drive, Room 5509, Bethesda, MD 20892, USA
J Immunol Methods 274:221-8. 2003..In addition, our results guide the design and implementation of the experiments to deconvolute the responses to individual peptide epitopes... - Measuring containment of viable infectious cell sorting in high-velocity cell sortersStephen P Perfetto
Vaccine Research Center, NAID, National Institute of Health, Bethesda, Maryland 20892 3015, USA
Cytometry A 52:122-30. 2003..With the advent of high-speed sorters, aerosols are a considerable safety concern when sorting viable infectious materials. We describe a four-part safety procedure for validating the containment... - Dynamic bookmarking of primary response genes by p300 and RNA polymerase II complexesJung S Byun
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:19286-91. 2009.... - Effects of exogenous interleukin-7 on human thymus functionYukari Okamoto
Vaccine Research Center, National Institute of Allergy and Infectious Diseases and Department of Experimental Transplantation and Immunology, Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
Blood 99:2851-8. 2002..Our results provide compelling evidence that IL-7 has a direct effect on increasing TCR-alphabeta rearrangement and indicate the potential use of IL-7 for enhancing de novo naïve T-cell generation in immunocompromised patients... - Long-lived memory T lymphocyte responses against SARS coronavirus nucleocapsid protein in SARS-recovered patientsHui Peng
Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II, Guangzhou 510089, China
Virology 351:466-75. 2006.... - Human memory T cell responses to SARS-CoV E proteinHui Peng
Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, No. 74 Zhongshan Road II, Guangzhou 510089, China
Microbes Infect 8:2424-31. 2006.... - Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patientsLi-tao Yang
Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China
Clin Immunol 120:171-8. 2006..These data may have an important implication in the possibility of designing effective vaccine against SARS-CoV infection, specifically in defining T-cell populations that are implicated in protective immunity... - Antiretroviral-drug resistance among patients recently infected with HIVSusan J Little
Antiviral Research Center, Department of Medicine, University of California San Diego, San Diego 92103, USA
N Engl J Med 347:385-94. 2002..Among persons in North America who are newly infected with the human immunodeficiency virus (HIV), the prevalence of transmitted resistance to antiretroviral drugs has been estimated at 1 to 11 percent... - Nonpathogenic SIV infection of sooty mangabeys is characterized by limited bystander immunopathology despite chronic high-level viremiaGuido Silvestri
Departments of Medicine and Microbiology and Immunology and, Emory University School of Medicine, Atlanta, GA, USA
Immunity 18:441-52. 2003..Rather, attenuated immune activation enables SIV-infected mangabeys to avoid the bystander damage seen in pathogenic infections and protects them from developing AIDS... - T cells containing T cell receptor excision circles are inversely related to HIV replication and are selectively and rapidly released into circulation with antiretroviral treatmentMireya Diaz
Department of Epidemiology and Biostatistics, Case Western Reserve University, Cleveland, Ohio 44106, USA
AIDS 17:1145-9. 2003..CONCLUSIONS: These findings suggest that recent thymic emigrants are sequestered in lymphoid tissue during uncontrolled HIV replication and are selectively released into circulation rapidly after initiation of antiretroviral therapies... - Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cellsPetra Wolint
Institute for Microbiology, , Schmelzbergstrasse 7, 8092, Switzerland
J Exp Med 199:925-36. 2004..Furthermore, these results provide a potential mechanism by which central memory CD8+ T cell-mediated death of antigen-presenting cells within the lymph node is avoided... - Glatiramer acetate (Copaxone) therapy induces CD8(+) T cell responses in patients with multiple sclerosisNitin J Karandikar
Department of Pathology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9072, USA
J Clin Invest 109:641-9. 2002.... - Dendritic cells are less susceptible to human immunodeficiency virus type 2 (HIV-2) infection than to HIV-1 infectionMelody G Duvall
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
J Virol 81:13486-98. 2007.... - Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populationsNatalie E R Beveridge
Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Eur J Immunol 37:3089-100. 2007..Overall, these data strongly support the use of MVA85A in humans as a boosting agent to expand polyfunctional M.tb-specific CD4(+) T cells capable of significant secondary responses... - Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 alleleGuido Ferrari
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
J Immunol 173:2126-33. 2004.... - Polyfunctional T cell responses are a hallmark of HIV-2 infectionMelody G Duvall
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
Eur J Immunol 38:350-63. 2008..Polyfunctional HIV-specific T cell responses are a hallmark of non-progressive HIV-2 infection and may be related to good clinical outcome in this setting... - Differential susceptibility to human immunodeficiency virus type 1 infection of myeloid and plasmacytoid dendritic cellsAnna Smed-Sorensen
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, F59 Huddinge University Hospital, S 141 86 Stockholm, Sweden
J Virol 79:8861-9. 2005..The difference in susceptibility of PDCs and MDCs to HIV-1 may have implications for HIV-1 transmission and DC-mediated transfer of HIV-1 to T cells... - Correlates of immune protection in HIV-1 infection: what we know, what we don't know, what we should knowGiuseppe Pantaleo
Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland
Nat Med 10:806-10. 2004..We will also discuss why it is important to design phase 3 clinical trials of HIV vaccines to determine the correlates of protection for each individual vaccine... - High prevalence of autoreactive, neuroantigen-specific CD8+ T cells in multiple sclerosis revealed by novel flow cytometric assayMichael P Crawford
Department of Pathology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9072, USA
Blood 103:4222-31. 2004..The results emphasize the need to evaluate both CD4(+) and CD8(+) T-cell responses in MS and to make both subsets a consideration in the development of novel therapeutic strategies... - Maintenance of HIV-specific CD4+ T cell help distinguishes HIV-2 from HIV-1 infectionMelody G Duvall
Medical Research Council (MRC) Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
J Immunol 176:6973-81. 2006..This study demonstrates that HIV-2-infected donors have a well-preserved and functionally heterogeneous HIV-specific memory CD4+ T cell response that is associated with delayed disease progression in the majority of infected people... - Multiclade human immunodeficiency virus type 1 envelope immunogens elicit broad cellular and humoral immunity in rhesus monkeysMichael S Seaman
Beth Israel Deaconess Medical Center, Division of Viral Pathogenesis, 330 Brookline Ave./RE-113, Boston, MA 02215, USA
J Virol 79:2956-63. 2005..This study demonstrates that it is possible to generate protective immune responses by vaccination with genetically diverse isolates of HIV-1... - Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34(+) cellsGreg M Podsakoff
Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA 90027, USA
Mol Ther 12:77-86. 2005..These findings indicate that there was a selective survival advantage for PBMC containing the huM10 gene during the time of increased HIV-1 load... - Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigenLitao Yang
Department of Immunology, Zhongshan Medical School, Sun Yat Sen University, Guangzhou 510089, China
J Gen Virol 88:2740-8. 2007..All four immunodominant peptides could elicit cellular immunity with a predominance of CD8+ T-cell response. This data may have important implication for developing SARS vaccines... - Adult human liver contains CD8pos T cells with naive phenotype, but is not a site for conventional alpha beta T cell developmentLucy Golden-Mason
Education and Research Center, St Vincent s University Hospital, Dublin, Ireland
J Immunol 172:5980-5. 2004..The almost complete absence of TRECs suggests that normal AHL is not a site for the development of conventional alphabeta T cells...