Leigh A Knodler

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Structure-based mutagenesis of SigE verifies the importance of hydrophobic and electrostatic residues in type III chaperone function
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Mol Microbiol 62:928-40. 2006
  2. pmc Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia
    Leigh A Knodler
    Laboratory of Intracellular Parasites and Research Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 107:17733-8. 2010
  3. pmc Coiled-coil domains enhance the membrane association of Salmonella type III effectors
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA
    Cell Microbiol 13:1497-517. 2011
  4. ncbi request reprint Salmonella type III effectors PipB and PipB2 are targeted to detergent-resistant microdomains on internal host cell membranes
    Leigh A Knodler
    Biotechnology Laboratory, University of British Columbia, Vancouver, BC, Canada
    Mol Microbiol 49:685-704. 2003
  5. pmc Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Cell Microbiol 11:1652-70. 2009
  6. pmc The Salmonella effector PipB2 affects late endosome/lysosome distribution to mediate Sif extension
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Mol Biol Cell 16:4108-23. 2005
  7. pmc Brucella modulates secretory trafficking via multiple type IV secretion effector proteins
    Sebenzile Myeni
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    PLoS Pathog 9:e1003556. 2013
  8. ncbi request reprint The mechanism of Salmonella entry determines the vacuolar environment and intracellular gene expression
    Dan Drecktrah
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Traffic 7:39-51. 2006
  9. pmc Salmonella trafficking is defined by continuous dynamic interactions with the endolysosomal system
    Dan Drecktrah
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Traffic 8:212-25. 2007
  10. pmc Toll-like receptor 4 contributes to colitis development but not to host defense during Citrobacter rodentium infection in mice
    Mohammed A Khan
    Division of Gastroenterology, BC s Children s Hospital, Vancouver, British Columbia V6H 3V4, Canada
    Infect Immun 74:2522-36. 2006

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Structure-based mutagenesis of SigE verifies the importance of hydrophobic and electrostatic residues in type III chaperone function
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Mol Microbiol 62:928-40. 2006
    ..Our results highlight that both hydrophobic and electronegative surfaces are required for the function of SigE and provide an important basis for the prediction of side-chain requirements for other chaperone-effector pairs...
  2. pmc Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia
    Leigh A Knodler
    Laboratory of Intracellular Parasites and Research Technologies Branch, Microscopy Unit, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 107:17733-8. 2010
    ....
  3. pmc Coiled-coil domains enhance the membrane association of Salmonella type III effectors
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA
    Cell Microbiol 13:1497-517. 2011
    ..These findings suggest that coiled-coil domains represent a common membrane-targeting determinant for Salmonella type III effectors...
  4. ncbi request reprint Salmonella type III effectors PipB and PipB2 are targeted to detergent-resistant microdomains on internal host cell membranes
    Leigh A Knodler
    Biotechnology Laboratory, University of British Columbia, Vancouver, BC, Canada
    Mol Microbiol 49:685-704. 2003
    ..The enrichment of Salmonella effectors in DRMs on these intracellular membranes probably permits specific interactions with host cell molecules that are concentrated in these signalling platforms...
  5. pmc Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Cell Microbiol 11:1652-70. 2009
    ..Altogether, our data show that ubiquitination of SopB is not related to its intracellular stability but rather regulates its enzymatic activity at the plasma membrane and intracellular localization...
  6. pmc The Salmonella effector PipB2 affects late endosome/lysosome distribution to mediate Sif extension
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Mol Biol Cell 16:4108-23. 2005
    ..Therefore, it seems that a divergence in the biological functions of these two effectors can be accounted for by sequence divergence in their C termini...
  7. pmc Brucella modulates secretory trafficking via multiple type IV secretion effector proteins
    Sebenzile Myeni
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    PLoS Pathog 9:e1003556. 2013
    ..Taken together, these findings demonstrate that Brucella modulates secretory trafficking via multiple T4SS effector proteins that likely act coordinately to promote Brucella pathogenesis...
  8. ncbi request reprint The mechanism of Salmonella entry determines the vacuolar environment and intracellular gene expression
    Dan Drecktrah
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Traffic 7:39-51. 2006
    ..Unexpectedly, SPI1 genes, which are rapidly downregulated following SPI1-mediated invasion, were induced intracellularly following phagocytic uptake. These results reveal another level of complexity in pathogen-macrophage interactions...
  9. pmc Salmonella trafficking is defined by continuous dynamic interactions with the endolysosomal system
    Dan Drecktrah
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Traffic 8:212-25. 2007
    ..These results provide compelling evidence that inhibition of SCV-lysosome fusion is not the major determinant in establishment of the Salmonella replicative niche in epithelial cells...
  10. pmc Toll-like receptor 4 contributes to colitis development but not to host defense during Citrobacter rodentium infection in mice
    Mohammed A Khan
    Division of Gastroenterology, BC s Children s Hospital, Vancouver, British Columbia V6H 3V4, Canada
    Infect Immun 74:2522-36. 2006
    ....
  11. pmc Cloning vectors and fluorescent proteins can significantly inhibit Salmonella enterica virulence in both epithelial cells and macrophages: implications for bacterial pathogenesis studies
    Leigh A Knodler
    Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Infect Immun 73:7027-31. 2005
    ..Here we show that they can impair the ability of Salmonella enterica serovar Typhimurium to productively infect either cultured mammalian cells or mice. This has significant implications for studies that rely on these systems...
  12. doi request reprint Brucella intracellular replication requires trafficking through the late endosomal/lysosomal compartment
    Tregei Starr
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Traffic 9:678-94. 2008
    ..Taken together, these results demonstrate that BCVs traffic along the endocytic pathway and fuse with lysosomes, and such fusion events are required for further maturation of BCVs into an ER-derived replicative organelle...
  13. ncbi request reprint Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems
    Leigh A Knodler
    Biotechnology Laboratory, Room 237, 6174 University Blvd, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 43:1089-103. 2002
    ..Thus, we demonstrate a functional and regulatory cross-talk between three chromosomal PAIs, SPI-1, SPI-2 and SPI-5, which has significant implications for the evolution and role of PAIs in bacterial pathogenesis...
  14. ncbi request reprint Taking possession: biogenesis of the Salmonella-containing vacuole
    Leigh A Knodler
    Host Parasite Interactions Section, Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases NIH, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Traffic 4:587-99. 2003
    ..In this review we discuss the recent advances in determining the mechanisms by which Salmonella regulate Salmonella-containing vacuole biogenesis and the implications of these findings...
  15. pmc Activation of Akt by the bacterial inositol phosphatase, SopB, is wortmannin insensitive
    Kendal G Cooper
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Disease, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, United States of America
    PLoS ONE 6:e22260. 2011
    ..Altogether these data demonstrate that Salmonella activates Akt via a wortmannin insensitive mechanism that is likely a class I PI3K-independent process that incorporates some essential elements of the canonical pathway...
  16. ncbi request reprint SopD2 is a novel type III secreted effector of Salmonella typhimurium that targets late endocytic compartments upon delivery into host cells
    John H Brumell
    Biotechnology Laboratory and Departments of Biochemistry and Molecular Biology, Microbiology and Immunology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Traffic 4:36-48. 2003
    ..These findings demonstrate that the N-terminus of SopD2 is a bifunctional domain required for both type III secretion out of Salmonella as well as late endosome/lysosome targeting following translocation into host cells...
  17. pmc Dynamic behavior of Salmonella-induced membrane tubules in epithelial cells
    Dan Drecktrah
    Laboratory of Intracellular Parasites, NIAID, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Traffic 9:2117-29. 2008
    ..Together, these results show that these Salmonella-induced tubules form a highly dynamic network that involves both microtubule-dependent motility and interactions with endosomal compartments...
  18. pmc Induction of Salmonella pathogenicity island 1 under different growth conditions can affect Salmonella-host cell interactions in vitro
    J Antonio Ibarra
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Microbiology 156:1120-33. 2010
    ..These findings show that induction of SPI1 under different pre-invasion growth conditions can affect the ability of Salmonella to interact with eukaryotic host cells...
  19. ncbi request reprint The invasion-associated type III secretion system of Salmonella enterica serovar Typhimurium is necessary for intracellular proliferation and vacuole biogenesis in epithelial cells
    Olivia Steele-Mortimer
    Biotechnology Laboratory, University of British Columbia, Vancouver BC V6T1Z3, Canada
    Cell Microbiol 4:43-54. 2002
    ..Typhimurium This demonstrates a previously unknown role for SPI-1 in vacuole biogenesis and intracellular survival in non-phagocytic cells...
  20. ncbi request reprint Salmonella enterica serovar Typhimurium effector SigD/SopB is membrane-associated and ubiquitinated inside host cells
    Sandra L Marcus
    Biotechnology Laboratory, University of British Columbia, 237 Wesbrook Building, 6174 University Boulevard, Vancouver, BC, V6T 1Z3, Canada
    Cell Microbiol 4:435-46. 2002
    ..We speculate that ubiquitination serves to downregulate SigD activity by an alternative mechanism, such as by targeting it for lysosomal degradation...
  21. ncbi request reprint The Salmonella effector protein SopB protects epithelial cells from apoptosis by sustained activation of Akt
    Leigh A Knodler
    Laboratory of Intracellular Parasites, NIAID, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    J Biol Chem 280:9058-64. 2005
    ..These results provide new insights into the mechanisms of apoptosis and highlight how bacterial effectors can intercept signaling pathways to manipulate host responses...
  22. pmc Bacterial colonization of host cells in the absence of cholesterol
    Stacey D Gilk
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America
    PLoS Pathog 9:e1003107. 2013
    ..These data demonstrate that cholesterol is not essential for invasion and intracellular replication by S. Typhimurium and C. trachomatis, but plays a role in C. burnetii-host cell interactions...
  23. pmc Modulation and utilization of host cell phosphoinositides by Salmonella spp
    Dan Drecktrah
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 72:4331-5. 2004
  24. ncbi request reprint The Salmonella SPI1 effector SopB stimulates nitric oxide production long after invasion
    Dan Drecktrah
    Laboratory of Intracellular Parasites, National Institutes of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Cell Microbiol 7:105-13. 2005
    ..Furthermore, SopB protein and message persist many hours after invasion. Our findings reveal an unanticipated potential for dialogue between the SPI1 and SPI2 TTSS and the host cell response...
  25. doi request reprint Of microbes and membranes: pathogenic subversion of host cell processes
    Jean Celli
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Cell Host Microbe 4:514-8. 2008
    ..Work presented at this meeting highlighted how pathogens exploit host cell membrane processes to their advantage and also revealed fundamental signaling and trafficking mechanisms of eukaryotic cells...
  26. pmc Mice lacking T and B lymphocytes develop transient colitis and crypt hyperplasia yet suffer impaired bacterial clearance during Citrobacter rodentium infection
    Bruce A Vallance
    Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    Infect Immun 70:2070-81. 2002
    ..While T and/or B lymphocytes are essential for host defense against C. rodentium, they also mediate much of the tissue pathology and disease symptoms that occur during infection...
  27. pmc Quantitative assessment of cytosolic Salmonella in epithelial cells
    Leigh A Knodler
    Paul G Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America
    PLoS ONE 9:e84681. 2014
    ..Together, digitonin permeabilization and the chloroquine resistance assay will be useful, complementary tools for deciphering the mechanisms of SCV lysis and Salmonella replication in the epithelial cell cytosol. ..
  28. pmc Eating the strangers within: host control of intracellular bacteria via xenophagy
    Leigh A Knodler
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Cell Microbiol 13:1319-27. 2011
    ..Here we review recent advances in our molecular understanding of these processes, and in how bacteria have adapted to avoid xenophagy or even take advantage of this innate immune process...
  29. pmc The Salmonella effector protein PipB2 is a linker for kinesin-1
    Thomas Henry
    Centre d immunologie de Marseille Luminy, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Universite de la Mediterranee, Parc Scientifique de Luminy, Marseille, France
    Proc Natl Acad Sci U S A 103:13497-502. 2006
    ..Taken together, our data indicate that the TTSS-2-mediated fine-tuning of kinesin-1 activity associated with the bacterial vacuole is crucial for the virulence of Salmonella...
  30. pmc HilD-mediated transcriptional cross-talk between SPI-1 and SPI-2
    Víctor H Bustamante
    Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 2001, colonia Chamilpa Cuernavaca, Morelos 62210, Mexico
    Proc Natl Acad Sci U S A 105:14591-6. 2008
    ..Our results suggest that two independent SPI-2 activation pathways evolved to take advantage of the SPI-2-encoded information at different niches and, in consequence, in response to different growth conditions...