Research Topics
Genomes and Genes
| Mark A KnepperSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Peter Agre, 2003 Nobel Prize winner in chemistryMark A Knepper
National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 6N260, 10 Center Drive MSC 1603, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 15:1093-5. 2004..Agre's discovery of the first water channel has spurred a revolution in animal and plant physiology and in medicine...
Systems biology in physiology: the vasopressin signaling network in kidneyMark A Knepper
National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Cell Physiol 303:C1115-24. 2012..The ultimate goal is to use multivariate statistical techniques and differential equations to obtain predictive models describing vasopressin signaling in the renal collecting duct...- Renal aquaporinsM A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Kidney Int 49:1712-7. 1996..Short-term regulation of collecting duct water permeability by vasopressin is largely a consequence of regulated trafficking of AQP2-containing vesicles to and from the apical plasma membrane... - Renal tubule sodium transporter abundance profiling in rat kidney: response to aldosterone and variations in NaCl intakeMark A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Ann N Y Acad Sci 986:562-9. 2003..Here, we review some of our recent findings with this approach, focusing on renal responses to aldosterone and to variations in NaCl intake... - Concentration of solutes in the renal inner medulla: interstitial hyaluronan as a mechano-osmotic transducerMark A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Am J Physiol Renal Physiol 284:F433-46. 2003.... - Exosomes in urine: who would have thought...?M A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA
Kidney Int 72:1043-5. 2007..These exosomes, demonstrated by Keller and colleagues to be also retrievable from amniotic fluid, offer great promise for future disease biomarker discovery studies... - NHLBI-AbDesigner: an online tool for design of peptide-directed antibodiesTrairak Pisitkun
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Am J Physiol Cell Physiol 302:C154-64. 2012.... - Proteomics and the kidneyMark A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Am Soc Nephrol 13:1398-408. 2002..The purpose of this Science Watch article is to describe the fundamental features of these two approaches and to speculate on how proteomics will be useful in nephrology and nephrology research in the coming years... - Regulation of aquaporin-2 water channel trafficking by vasopressinM A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 0951, USA
Curr Opin Cell Biol 9:560-4. 1997..The vesicle-targeting proteins synaptobrevin-2 and syntaxin-4 are proposed to play roles in this process... - Regulation of the sodium transporters NHE3, NKCC2 and NCC in the kidneyM A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Curr Opin Nephrol Hypertens 10:655-9. 2001.... - Role of aquaporins in water balance disordersM A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1598, USA
Curr Opin Nephrol Hypertens 6:367-71. 1997.... - Long-term regulation of ENaC expression in kidney by angiotensin IIKathleen T Beutler
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, MD 20892-1603, USA
Hypertension 41:1143-50. 2003..The results support the view that the angiotensin II receptor regulates ENaC abundance, consistent with a role for angiotensin II in regulation of collecting duct function... - Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cellsRuwan Gunaratne
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:15653-8. 2010..These results support the view that, although protein kinase A plays a central role in mTAL signaling, additional kinases, including those that target proline-directed motifs, may be involved... - Vasopressin increases phosphorylation of Ser84 and Ser486 in Slc14a2 collecting duct urea transportersShelly Hwang
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Renal Physiol 299:F559-67. 2010..The findings add to prior evidence for vasopressin-induced phosphorylation of UT-A1, providing evidence that UT-A3 may be regulated by phosphorylation as well... - Akt and ERK1/2 pathways are components of the vasopressin signaling network in rat native IMCDTrairak Pisitkun
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 295:F1030-43. 2008..Based on the current findings integrated with previous findings in the IMCD, we now report a 33-node vasopressin signaling network involved in vasopressin regulation of IMCD function... - LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cellsMing Jiun Yu
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Proteomics 5:2131-45. 2006..A World Wide Web-accessible database was constructed of 222 membrane proteins (integral and GPI-linked) from this study and prior studies... - Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cellsMarkus M Rinschen
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:3882-7. 2010.... - Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cellsSookkasem Khositseth
Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
Mol Cell Proteomics 10:M110.004036. 2011..The results provide impetus for increased focus on translational regulation and regulation of protein degradation in physiological control in mammalian epithelial cells... - Time course of renal Na-K-ATPase, NHE3, NKCC2, NCC, and ENaC abundance changes with dietary NaCl restrictionShyama Masilamani
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Am J Physiol Renal Physiol 283:F648-57. 2002.... - Gamble's "economy of water" revisited: studies in urea transporter knockout miceRobert A Fenton
Laboratory of Kidney and Electrolyte Metabolism, National Hearth, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA, and Faculty of Life Sciences, University of Manchester, UK
Am J Physiol Renal Physiol 291:F148-54. 2006.... - Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting ductAmar D Bansal
National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 21:303-15. 2010..These results expand the known list of collecting duct phosphoproteins and highlight the utility of targeted phosphoproteomic approaches... - Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2Jason D Hoffert
NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 283:24617-27. 2008..The data support a model in which vasopressin-mediated phosphorylation of AQP2 at Ser269:(a) depends on prior PKA-mediated phosphorylation of Ser256 and (b) enhances apical plasma membrane retention of AQP2... - Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting ductMing Jiun Yu
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Am J Physiol Cell Physiol 295:C661-78. 2008.... - Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting ductJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 292:F691-700. 2007.... - Quantitative analysis of aquaporin-2 phosphorylationLuke Xie
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Am J Physiol Renal Physiol 298:F1018-23. 2010..We suggest from these studies that Ser269 phosphorylation may be a more consistent indicator of vasopressin action and AQP2 membrane abundance than is Ser256 phosphorylation... - Increased collecting duct urea transporter expression in Dahl salt-sensitive ratsRobert A Fenton
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and BIood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 285:F143-51. 2003.... - Regulation of NHE3, NKCC2, and NCC abundance in kidney during aldosterone escape phenomenon: role of NOSharon Turban
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Am J Physiol Renal Physiol 285:F843-51. 2003..We conclude that NHE3 and NKCC2 protein abundances in kidney are positively regulated by NO and that the increase in NHE3 abundance seen in the aldosterone escape phenomenon is NO dependent... - An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteinsJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, Proteomics Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
J Proteome Res 6:3501-8. 2007..Using a label-free quantification approach, we identified phosphoproteins that changed in abundance with vasopressin exposure including aquaporin-2 (AQP2), Hnrpa3, IP3 receptor 3, and pur-beta... - Large-scale proteomics and phosphoproteomics of urinary exosomesPatricia A Gonzales
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Am Soc Nephrol 20:363-79. 2009..The proteomic data are publicly accessible at http://dir.nhlbi.nih.gov/papers/lkem/exosome/... - Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting ductMaria Barile
Laboratory of Kidney and Electrolyte Metabolism and Proteomics Core Facility, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Proteomics 4:1095-106. 2005..These results show that AQP2-containing vesicles are heterogeneous and that intracellular AQP2 resides chiefly in endosomes, trans-Golgi network, and rough endoplasmic reticulum... - Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSnGuanghui Wang
Proteomics Core Facility and Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Chem 78:5752-61. 2006..Compared with a widely used commercial software tool, QUIL showed improvement in ion chromatogram construction and peak integration and significantly reduced relative errors in abundance ratio assessment... - Sodium transporter abundance profiling in kidney: effect of spironolactoneJakob Nielsen
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603, USA
Am J Physiol Renal Physiol 283:F923-33. 2002.... - LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of ratAaron N Sachs
National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 295:F1799-806. 2008.... - Quantitative proteomics identifies vasopressin-responsive nuclear proteins in collecting duct cellsLaura K Schenk
National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 23:1008-18. 2012..The findings provide a new online database resource for nuclear proteomics (http://helixweb.nih.gov/ESBL/Database/mNPD/) and generate new hypotheses regarding vasopressin-mediated transcriptional regulation in the collecting duct... - Systems-level analysis of cell-specific AQP2 gene expression in renal collecting ductMing Jiun Yu
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:2441-6. 2009..The results implicate ETS family TRs in cell-specific expression of AQP2 and point to HOX, RXR, CREB and GATA family TRs as playing likely additional roles... - Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MSDmitry Tchapyjnikov
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Physiol Genomics 40:167-83. 2010..The newly identified proteins have been incorporated into the IMCD Proteome Database (http://dir.nhlbi.nih.gov/papers/lkem/imcd/)... - Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomicsJason D Hoffert
Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Proteomics 11:M111.014613. 2012..Finally, we provide an online resource of physiologically regulated phosphorylation sites with dynamic quantitative data (http://helixweb.nih.gov/ESBL/Database/TiPD/index.html)... - Identification and quantification of basic and acidic proteins using solution-based two-dimensional protein fractionation and label-free or 18O-labeling mass spectrometryWells W Wu
Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Proteome Res 6:2447-59. 2007..With the use of HCT116 colon carcinoma cells, a total of 305 basic and 183 acidic proteins was identified. Quantitative proteomics revealed that 17 of these proteins were differentially expressed in HCT116 p53-/- cells... - Non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting ductChung Lin Chou
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 279:49026-35. 2004.... - Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting ductChung Lin Chou
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Am J Physiol Renal Physiol 295:F192-201. 2008..We conclude that the AVP-induced cell height increase is dependent on basolateral solute uptake via NKCC1 and changes in actin organization via myosin II, but is not dependent specifically on increased apical water entry... - PhosphoScore: an open-source phosphorylation site assignment tool for MSn dataBrian E Ruttenberg
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
J Proteome Res 7:3054-9. 2008..PhosphoScore produced >95% correct MS(2) assignments from known synthetic data, > 98% agreement with an established MS(2) assignment algorithm (Ascore), and >92% agreement with visual inspection of MS(3) and MS(4) spectra... - Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro ratBas W M van Balkom
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 286:F216-24. 2004.... - A selective EP4 PGE2 receptor agonist alleviates disease in a new mouse model of X-linked nephrogenic diabetes insipidusJian Hua Li
Molecular Signaling Section, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA
J Clin Invest 119:3115-26. 2009..These findings illustrate the usefulness of the newly generated V2R mutant mice for elucidating and testing new strategies for the potential treatment of humans with XNDI... - Calmodulin is required for vasopressin-stimulated increase in cyclic AMP production in inner medullary collecting ductJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 280:13624-30. 2005..These studies demonstrate the importance of calmodulin in the regulation of collecting duct adenylyl cyclase activity and transport function... - An online tool for calculation of free-energy balance for the renal inner medullaRyan L Vilbig
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 303:F366-72. 2012..Its use is illustrated by calculating external free-energy balance for an example of the passive concentrating model taken from the original paper by Kokko and Rector (Kokko JP, Rector FC Jr. Kidney Int 2: 214-223, 1972)... - Renal phenotype of UT-A urea transporter knockout miceRobert A Fenton
Laboratory of Kidney Electrolyte Metabolism, National Heart, Lung and Blood Institutes, National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 16:1583-92. 2005..These results support our conclusion that the urinary concentrating defect in UT-A1/3(-/-) mice is caused by a failure of urea transport from the IMCD lumen to the inner medullary interstitium, resulting in osmotic diuresis... - Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sitesJason D Hoffert
National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:7159-64. 2006.... - High-throughput identification of IMCD proteins using LC-MS/MSTrairak Pisitkun
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Physiol Genomics 25:263-76. 2006..A 28-protein vasopressin signaling network was constructed using literature-based network analysis software focusing on the newly identified proteins, providing several new hypotheses for future studies... - Altered expression profile of transporters in the inner medullary collecting duct of aquaporin-1 knockout miceRyan G Morris
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 289:F194-9. 2005..WT controls. In conclusion, the expression profile of IMCD transporters is markedly altered in AQP1 -/- mice and this manifestation is related to the associated concentrating defect... - Hypokalemia in a mouse model of Gitelman's syndromeRyan G Morris
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10, Rm 6N260, 10 Center Dr MSC 1603, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 290:F1416-20. 2006..Therefore, NCC (-/-) mice are more sensitive to reductions in dietary potassium than wild-type mice and become hypokalemic, thus more faithfully representing the Gitelman phenotype seen in humans... 
Isolation and purification of exosomes in urinePatricia A Gonzales
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 641:89-99. 2010..Validation methods include western blots of pan-exosome markers and segment-specific exosome markers, and negative staining electron microscopy...- Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometryNicholas A Zwang
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Proteome Res 8:1540-54. 2009..These results suggest that phosphorylation of AQP2 at Ser-256 may regulate AQP2 trafficking in part by mediating differential binding of hsp70 family proteins to the COOH-terminal tail... - The application of DIGE-based proteomics to renal physiologyEwout J Hoorn
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. 20892, USA
Nephron Physiol 104:p61-72. 2006..Finally, we illustrate how quantification based on the DIGE approach combined with bioinformatics may facilitate the study of systems biology of the kidney... - Quantitative phosphoproteomics in nuclei of vasopressin-sensitive renal collecting duct cellsSteven J Bolger
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Cell Physiol 303:C1006-20. 2012..All data are available to users at http://helixweb.nih.gov/ESBL/Database/mNPPD/... - Transcriptional profiling of native inner medullary collecting duct cells from rat kidneyPanapat Uawithya
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland
Physiol Genomics 32:229-53. 2008..Altogether, the results combine with proteomics studies of the IMCD to provide a framework for modeling complex interaction networks responsible for vasopressin action in collecting duct cells... - Proteomic approaches for the study of cell signaling in the renal collecting ductEwout J Hoorn
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Contrib Nephrol 160:172-85. 2008..We also emphasize that proteomics of body fluids will be the strategy to identify disease biomarkers, and therefore conclude the review by highlighting the perspectives of biomarker discovery in urinary exosomes... - Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting ductRobert A Fenton
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
Proc Natl Acad Sci U S A 101:7469-74. 2004..Thus, these results do not corroborate the predictions of passive medullary concentrating models stating that NaCl accumulation in the inner medulla depends on rapid vasopressin-regulated urea transport across the IMCD epithelium... - Magnetic resonance imaging of urea transporter knockout mice shows renal pelvic abnormalitiesVinitha A Jacob
Laboratory of Kidney and Electrolyte Metabolism, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Kidney Int 74:1202-8. 2008..Our studies show that real time imaging of renal pelvic structure in genetically manipulated mice provides a tool for the non-destructive, temporal evaluation of kidney structure... - Application of difference gel electrophoresis to the identification of inner medullary collecting duct proteinsJason D Hoffert
Laboratory of Kidney and Electrolyte Mechanism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Rm. 6N260, MSC 1603, 10 Center Drive, Bethesda, MD 20892-1603, USA
Am J Physiol Renal Physiol 286:F170-9. 2004..These studies document the applicability of a standardized approach to purification of IMCD cells for proteomic analysis of IMCD proteins and demonstrate the feasibility of large scale identification of proteins in the native IMCD cell... - Prospects for urinary proteomics: exosomes as a source of urinary biomarkersEwout J Hoorn
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603, USA
Nephrology (Carlton) 10:283-90. 2005..Finally, potential barriers that need to be overcome before urinary proteomics can be applied clinically, are emphasized... - Gene expression databases for kidney epithelial cellsJennifer C Huling
National Institutes of Health 10 Center Dr Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 302:F401-7. 2012..Here, we provide a roadmap to these databases and illustrate how they may be useful in the design and interpretation of physiological studies. The databases can be accessed through http://helixweb.nih.gov/ESBL/Database... - Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubuleMarina Feric
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Cell Physiol 300:C755-70. 2011..An online database from this study (http://dir.nhlbi.nih.gov/papers/lkem/rcmpd/) provides a resource for future studies of transporter regulation... - Tandem mass spectrometry in physiologyTrairak Pisitkun
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Physiology (Bethesda) 22:390-400. 2007..Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology... - cDNA array identification of genes regulated in rat renal medulla in response to vasopressin infusionHeddwen L Brooks
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Am J Physiol Renal Physiol 284:F218-28. 2003..These studies have identified several transcripts whose abundances are regulated in the inner medulla in response to infusion of dDAVP and that could play roles in the regulation of salt and water excretion... - In vacuo isotope coded alkylation technique (IVICAT); an N-terminal stable isotopic label for quantitative liquid chromatography/mass spectrometry proteomicsBrigitte L Simons
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Rapid Commun Mass Spectrom 20:2463-77. 2006..IVICAT labeling, when used in conjunction with the new proteomics software QUIL, can accurately report relative protein abundances and increase the sequence coverage of proteins of tissue proteomes... - Enrichment of distinct microfilament-associated and GTP-binding-proteins in membrane/microvilli fractions from lymphoid cellsJian Jiang Hao
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Proteome Res 7:2911-27. 2008.... - Tamm-Horsfall protein and urinary exosome isolationPatrícia Fernández-Llama
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Kidney Int 77:736-42. 2010.... - Large-scale phosphotyrosine proteomic profiling of rat renal collecting duct epithelium reveals predominance of proteins involved in cell polarity determinationBoyang Zhao
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Am J Physiol Cell Physiol 302:C27-45. 2012..In general, these findings mesh well with evidence that tyrosine phosphorylation plays a key role in epithelial polarity determination... - Phosphoproteomics of vasopressin signaling in the kidneyJason D Hoffert
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
Expert Rev Proteomics 8:157-63. 2011.... - Decreased abundance of collecting duct urea transporters UT-A1 and UT-A3 with ECF volume expansionXiao-Yan Wang
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Am J Physiol Renal Physiol 282:F577-84. 2002..The results support the view that ECF-related changes in serum urea concentration are mediated, at least in part, through altered urea transporter abundance... - Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medullaValery Grinevich
Section on Endocrine Physiology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 1862, USA
Kidney Int 65:54-62. 2004..To test this hypothesis, we examined the effect of lipopolysaccharide (LPS) injection on the expression of V2 receptors and aquaporin-2 in the kidney... - Taking aim at shotgun phosphoproteomicsJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Anal Biochem 375:1-10. 2008 - Targeted proteomic profiling of renal Na(+) transporter and channel abundances in angiotensin II type 1a receptor knockout miceHeddwen L Brooks
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1603, USA
Hypertension 39:470-3. 2002.... - Structural biology. The atomic architecture of a gas channelMark A Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Institutes of Health, Bethesda, MD 20892, USA
Science 305:1573-4. 2004 - UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting ductRobert A Fenton
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Renal Physiol 290:F188-95. 2006..These results demonstrate that 4.2 kb of the UT-Aalpha promoter is sufficient to drive expression of a heterologous reporter gene in a tissue-specific and cell-specific fashion in transgenic mice... - Database for renal collecting duct regulatory and transporter proteinsJohn Legato
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda 20892-2690, USA
Physiol Genomics 13:179-81. 2003..Extension of the database is dynamic and is done through a maintenance interface. This permits creation of new categories, updating of existing entries, and addition of new ones... - Identification and proteomic profiling of exosomes in human urineTrairak Pisitkun
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1603, USA
Proc Natl Acad Sci U S A 101:13368-73. 2004..The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine... - Effect of peristaltic contractions of the renal pelvic wall on solute concentrations of the renal inner medulla in the hamsterMary Ella C Pruitt
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603, USA
Am J Physiol Renal Physiol 290:F892-6. 2006..This procedure also significantly decreased inner medullary osmolality. We conclude that elimination of the contractions of the renal pelvic wall in the hamster significantly impairs inner medullary concentrating ability... - Combined proteomics and pathways analysis of collecting duct reveals a protein regulatory network activated in vasopressin escapeEwout J Hoorn
National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892, USA
J Am Soc Nephrol 16:2852-63. 2005..The results demonstrate a dominant protein regulatory network in IMCD cells that is altered in association with vasopressin escape, providing a new framework for further studies of signaling in IMCD... - Aquaporin-2 in the "-omics" eraJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 284:14683-7. 2009..Here we review this progress... - VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influxBrij B Singh
Secretory Physiology Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
Mol Cell 15:635-46. 2004..In aggregate, these data suggest that VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to carbachol-stimulation of Ca2+ influx... - Increased apical targeting of renal epithelial sodium channel subunits and decreased expression of type 2 11beta-hydroxysteroid dehydrogenase in rats with CCl4-induced decompensated liver cirrhosisSoo Wan Kim
The Water and Salt Research Center, University of Aarhus, Aarhus, Denmark DK 8000
J Am Soc Nephrol 16:3196-210. 2005..In conclusion, increased apical targeting of ENaC subunits combined with diminished abundance of 11betaHSD2 in the DCT2, CNT, and cortical collecting duct is likely to play a role in the sodium retaining stage of liver cirrhosis... - Expression of urea transporters in potassium-depleted mouse kidneyJu Young Jung
Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo Dong, Socho gu, Seoul 137 701, Korea
Am J Physiol Renal Physiol 285:F1210-24. 2003..We conclude that reduced expression of urea transporters may play a role in the impaired urine-concentrating ability associated with potassium deprivation... - Reduced expression of Na-K-2Cl cotransporter in medullary TAL in vitamin D-induced hypercalcemia in ratsWeidong Wang
Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark
Am J Physiol Renal Physiol 282:F34-44. 2002.... - Segment-specific ENaC downregulation in kidney of rats with lithium-induced NDIJakob Nielsen
The Water and Salt Research Center, Institute of Anatomy Bldg 233, University of Aarhus, DK 8000 Aarhus, Denmark
Am J Physiol Renal Physiol 285:F1198-209. 2003.... - Increased renal ENaC subunit and sodium transporter abundances in streptozotocin-induced type 1 diabetesJian Song
Division of Endocrinology and Metabolism, Department of Medicine, Georgetown University, Box 571412, Washington, DC 20057-1412, USA
Am J Physiol Renal Physiol 285:F1125-37. 2003..These comprehensive changes would be expected to decrease volume contraction accompanying large-solute and water losses associated with DM... - Effect of primary polydipsia on aquaporin and sodium transporter abundanceMelissa A Cadnapaphornchai
Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA
Am J Physiol Renal Physiol 285:F965-71. 2003..We conclude that the impaired urinary concentrating ability associated with primary POLY in rats is due to impaired osmotic equilibration in the collecting duct that is mediated primarily by decreased AQP-2 protein abundance... - Expression of urea transporters in the developing rat kidneyYoung Hee Kim
Department of Anatomy, Catholic University Medical College, Seoul, Korea, 137 701, Georgia 30322, USA
Am J Physiol Renal Physiol 282:F530-40. 2002..We conclude that the expression of urea transporters in short-loop DTL and DVR coincides with the development of the ability to produce a concentrated urine... - Altered expression of renal NHE3, TSC, BSC-1, and ENaC subunits in potassium-depleted ratsMarie-Louise Elkjaer
The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark
Am J Physiol Renal Physiol 283:F1376-88. 2002..Downregulation of BSC-1, TSC, and ENaC may contribute to the urinary concentrating defect, whereas upregulation of NHE3 may be compensatory to prevent urinary Na+ loss and/or to maintain intracellular pH levels... - Dysregulation of renal salt and water transport proteins in diabetic Zucker ratsCrystal A Bickel
Division of Endocrinology and Metabolism, Department of Medicine, Georgetown University, Washington DC 20007, USA
Kidney Int 61:2099-110. 2002..This potentially could reduce blood pressure by decreasing proximal tubule sodium reabsorption... - Oxytocin induces apical and basolateral redistribution of aquaporin-2 in rat kidneyUn Sil Jeon
Departmentof Internal Medicine, Gyeong-Sang National University, Chinju, Korea
Nephron Exp Nephrol 93:e36-45. 2003..Oxytocin may be one of the factors that accounts for vasopressin-independent AQP2 targeting in the kidney... - Regulation of collecting duct AQP3 expression: response to mineralocorticoidTae-Hwan Kwon
The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus C, Denmark
Am J Physiol Renal Physiol 283:F1403-21. 2002..Therefore, in the collecting duct aldosterone may regulate, at least in part, AQP3 expression in addition to regulating Na and K transport... - Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout miceMing Lu
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520 8026, USA
J Biol Chem 277:37881-7. 2002..Despite loss of ROMK expression, the normokalemic null mice exhibited significantly increased kaliuresis, indicating alternative mechanisms for K(+) absorption/secretion in the nephron... - Altered expression of urea transporters in response to ureteral obstructionChunling Li
The Water and Salt Research Center/Institute of Experimental Clinical Research, Aarhus Univ. Hospital-Skejby, DK-8200 Aarhus, Denmark
Am J Physiol Renal Physiol 286:F1154-62. 2004..In conclusion, reduced expression of UT-A1, UT-A3, and UT-B levels in both BUO and UUO rats suggests that urea transporters play important roles in the impaired urinary concentrating capacity in response to urinary tract obstruction... - Altered expression of major renal Na transporters in rats with unilateral ureteral obstructionChunling Li
The Water and Salt Research Center and Department of Cell Biology, Institute of Anatomy, University of Aarhus, Denmark
Am J Physiol Renal Physiol 284:F155-66. 2003.... - Rosiglitazone activates renal sodium- and water-reabsorptive pathways and lowers blood pressure in normal ratsJian Song
Department of Medicine, Georgetown University, Washington, DC 20057-1412, USA
J Pharmacol Exp Ther 308:426-33. 2004..We conclude that both increases in renal tubule transporter abundance and a decrease in glomerular filtration rate likely contribute to the RGZ-induced sodium retention... - Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidneyKi Young Na
Department of Internal Medicine, Seoul National University College of Medicine, Clinical Research Institute of Seoul National University Hospital, Chongno Gu, Seoul 110 744, South Korea
Am J Physiol Renal Physiol 292:F1701-9. 2007..This upregulation of H(+)-ATPase in the intercalated cells may be the result of tubular hypertrophy by diuretics... - Urea and renal function in the 21st century: insights from knockout miceRobert A Fenton
Water and Salt Research Center, Institute of Anatomy, Building 233 234, University of Aarhus, DK 8000 Aarhus, Denmark
J Am Soc Nephrol 18:679-88. 2007.... - COX-2 activity transiently contributes to increased water and NaCl excretion in the polyuric phase after release of ureteral obstructionRikke Nørregaard
The Water and Salt Research Center, University of Aarhus, DK 8200 Aarhus N, Denmark
Am J Physiol Renal Physiol 292:F1322-33. 2007.... - Sodium retention in cirrhotic rats is associated with increased renal abundance of sodium transporter proteinsPatrícia Fernández-Llama
Fundacio Puigvert, Renal Unit and Hypertension, Barcelona, Spain
Kidney Int 67:622-30. 2005..Liver cirrhosis with ascites is associated with a decrease in renal sodium excretion and therefore sodium retention... - Maintained ENaC trafficking in aldosterone-infused rats during mineralocorticoid and glucocorticoid receptor blockadeJakob Nielsen
The Water and Salt Research Center, Institute of Anatomy, Aarhus, Denmark
Am J Physiol Renal Physiol 292:F382-94. 2007..We provide new evidence for ENaC trafficking occurring independent of MR and GR activation in aldosterone-infused rats...