Mark A Knepper

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Proteomics and the kidney
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Am Soc Nephrol 13:1398-408. 2002
  2. ncbi request reprint Peter Agre, 2003 Nobel Prize winner in chemistry
    Mark A Knepper
    National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 6N260, 10 Center Drive MSC 1603, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 15:1093-5. 2004
  3. doi request reprint Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct
    Jason D Hoffert
    NIH Bldg 10, Rm 6N260, 10 Center Dr, Bethesda, MD 20892 1603
    Am J Physiol Renal Physiol 306:410-21. 2014
  4. doi request reprint Tolvaptan as a tool in renal physiology
    Carlos A Miranda
    National Institutes of Health, Bldg 10, Rm 6N260, 10 Center Dr, MSC 1603, Bethesda, MD 20892 1603
    Am J Physiol Renal Physiol 306:F359-66. 2014
  5. pmc Urea channel inhibitors: a new functional class of aquaretics
    Mark A Knepper
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Kidney Int 83:991-3. 2013
  6. pmc Systems biology in physiology: the vasopressin signaling network in kidney
    Mark A Knepper
    National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Cell Physiol 303:C1115-24. 2012
  7. ncbi request reprint Renal aquaporins
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Kidney Int 49:1712-7. 1996
  8. pmc NHLBI-AbDesigner: an online tool for design of peptide-directed antibodies
    Trairak Pisitkun
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Cell Physiol 302:C154-64. 2012
  9. ncbi request reprint Concentration of solutes in the renal inner medulla: interstitial hyaluronan as a mechano-osmotic transducer
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Renal Physiol 284:F433-46. 2003
  10. ncbi request reprint Renal tubule sodium transporter abundance profiling in rat kidney: response to aldosterone and variations in NaCl intake
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Ann N Y Acad Sci 986:562-9. 2003

Collaborators

Detail Information

Publications122 found, 100 shown here

  1. ncbi request reprint Proteomics and the kidney
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Am Soc Nephrol 13:1398-408. 2002
    ..The purpose of this Science Watch article is to describe the fundamental features of these two approaches and to speculate on how proteomics will be useful in nephrology and nephrology research in the coming years...
  2. ncbi request reprint Peter Agre, 2003 Nobel Prize winner in chemistry
    Mark A Knepper
    National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 6N260, 10 Center Drive MSC 1603, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 15:1093-5. 2004
    ..Agre's discovery of the first water channel has spurred a revolution in animal and plant physiology and in medicine...
  3. doi request reprint Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct
    Jason D Hoffert
    NIH Bldg 10, Rm 6N260, 10 Center Dr, Bethesda, MD 20892 1603
    Am J Physiol Renal Physiol 306:410-21. 2014
    ..This study represents the first "systems-wide" analysis of a "vaptan"-class drug and provides a wealth of new data regarding the effects of satavaptan on vasopressin-mediated phosphorylation events. ..
  4. doi request reprint Tolvaptan as a tool in renal physiology
    Carlos A Miranda
    National Institutes of Health, Bldg 10, Rm 6N260, 10 Center Dr, MSC 1603, Bethesda, MD 20892 1603
    Am J Physiol Renal Physiol 306:F359-66. 2014
    ..Specifically, tolvaptan almost completely inhibited the ability of vasopressin to increase AQP2 phosphorylation at Ser256, Ser264, and Ser269, while strongly inhibiting a vasopressin-induced decrease in AQP2 phosphorylation at Ser261...
  5. pmc Urea channel inhibitors: a new functional class of aquaretics
    Mark A Knepper
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Kidney Int 83:991-3. 2013
    ..Such compounds would potentially be useful in treatment of hyponatremic disorders. Here we review the physiological basis for the action of urea channel inhibitors in the kidney and assess their clinical potential...
  6. pmc Systems biology in physiology: the vasopressin signaling network in kidney
    Mark A Knepper
    National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Cell Physiol 303:C1115-24. 2012
    ..The ultimate goal is to use multivariate statistical techniques and differential equations to obtain predictive models describing vasopressin signaling in the renal collecting duct...
  7. ncbi request reprint Renal aquaporins
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Kidney Int 49:1712-7. 1996
    ..Short-term regulation of collecting duct water permeability by vasopressin is largely a consequence of regulated trafficking of AQP2-containing vesicles to and from the apical plasma membrane...
  8. pmc NHLBI-AbDesigner: an online tool for design of peptide-directed antibodies
    Trairak Pisitkun
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Cell Physiol 302:C154-64. 2012
    ....
  9. ncbi request reprint Concentration of solutes in the renal inner medulla: interstitial hyaluronan as a mechano-osmotic transducer
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Renal Physiol 284:F433-46. 2003
    ....
  10. ncbi request reprint Renal tubule sodium transporter abundance profiling in rat kidney: response to aldosterone and variations in NaCl intake
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Ann N Y Acad Sci 986:562-9. 2003
    ..Here, we review some of our recent findings with this approach, focusing on renal responses to aldosterone and to variations in NaCl intake...
  11. ncbi request reprint Exosomes in urine: who would have thought...?
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA
    Kidney Int 72:1043-5. 2007
    ..These exosomes, demonstrated by Keller and colleagues to be also retrievable from amniotic fluid, offer great promise for future disease biomarker discovery studies...
  12. ncbi request reprint Regulation of the sodium transporters NHE3, NKCC2 and NCC in the kidney
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Curr Opin Nephrol Hypertens 10:655-9. 2001
    ....
  13. ncbi request reprint Role of aquaporins in water balance disorders
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1598, USA
    Curr Opin Nephrol Hypertens 6:367-71. 1997
    ....
  14. ncbi request reprint Regulation of aquaporin-2 water channel trafficking by vasopressin
    M A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 0951, USA
    Curr Opin Cell Biol 9:560-4. 1997
    ..The vesicle-targeting proteins synaptobrevin-2 and syntaxin-4 are proposed to play roles in this process...
  15. ncbi request reprint Long-term regulation of ENaC expression in kidney by angiotensin II
    Kathleen T Beutler
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Hypertension 41:1143-50. 2003
    ..The results support the view that the angiotensin II receptor regulates ENaC abundance, consistent with a role for angiotensin II in regulation of collecting duct function...
  16. pmc Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells
    Ruwan Gunaratne
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:15653-8. 2010
    ..These results support the view that, although protein kinase A plays a central role in mTAL signaling, additional kinases, including those that target proline-directed motifs, may be involved...
  17. pmc Vasopressin increases phosphorylation of Ser84 and Ser486 in Slc14a2 collecting duct urea transporters
    Shelly Hwang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Renal Physiol 299:F559-67. 2010
    ..The findings add to prior evidence for vasopressin-induced phosphorylation of UT-A1, providing evidence that UT-A3 may be regulated by phosphorylation as well...
  18. ncbi request reprint Gamble's "economy of water" revisited: studies in urea transporter knockout mice
    Robert A Fenton
    Laboratory of Kidney and Electrolyte Metabolism, National Hearth, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA, and Faculty of Life Sciences, University of Manchester, UK
    Am J Physiol Renal Physiol 291:F148-54. 2006
    ....
  19. pmc Akt and ERK1/2 pathways are components of the vasopressin signaling network in rat native IMCD
    Trairak Pisitkun
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 295:F1030-43. 2008
    ..Based on the current findings integrated with previous findings in the IMCD, we now report a 33-node vasopressin signaling network involved in vasopressin regulation of IMCD function...
  20. pmc Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells
    Sookkasem Khositseth
    Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Mol Cell Proteomics 10:M110.004036. 2011
    ..The results provide impetus for increased focus on translational regulation and regulation of protein degradation in physiological control in mammalian epithelial cells...
  21. pmc Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct
    Amar D Bansal
    National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 21:303-15. 2010
    ..These results expand the known list of collecting duct phosphoproteins and highlight the utility of targeted phosphoproteomic approaches...
  22. pmc Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells
    Markus M Rinschen
    National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:3882-7. 2010
    ....
  23. ncbi request reprint Increased collecting duct urea transporter expression in Dahl salt-sensitive rats
    Robert A Fenton
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and BIood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 285:F143-51. 2003
    ....
  24. ncbi request reprint Time course of renal Na-K-ATPase, NHE3, NKCC2, NCC, and ENaC abundance changes with dietary NaCl restriction
    Shyama Masilamani
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Renal Physiol 283:F648-57. 2002
    ....
  25. pmc Quantitative analysis of aquaporin-2 phosphorylation
    Luke Xie
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 298:F1018-23. 2010
    ..We suggest from these studies that Ser269 phosphorylation may be a more consistent indicator of vasopressin action and AQP2 membrane abundance than is Ser256 phosphorylation...
  26. pmc Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2
    Jason D Hoffert
    NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 283:24617-27. 2008
    ..The data support a model in which vasopressin-mediated phosphorylation of AQP2 at Ser269:(a) depends on prior PKA-mediated phosphorylation of Ser256 and (b) enhances apical plasma membrane retention of AQP2...
  27. ncbi request reprint Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 292:F691-700. 2007
    ....
  28. pmc LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cells
    Ming Jiun Yu
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 5:2131-45. 2006
    ..A World Wide Web-accessible database was constructed of 222 membrane proteins (integral and GPI-linked) from this study and prior studies...
  29. pmc Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct
    Ming Jiun Yu
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Cell Physiol 295:C661-78. 2008
    ....
  30. ncbi request reprint Regulation of NHE3, NKCC2, and NCC abundance in kidney during aldosterone escape phenomenon: role of NO
    Sharon Turban
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 285:F843-51. 2003
    ..We conclude that NHE3 and NKCC2 protein abundances in kidney are positively regulated by NO and that the increase in NHE3 abundance seen in the aldosterone escape phenomenon is NO dependent...
  31. pmc Large-scale proteomics and phosphoproteomics of urinary exosomes
    Patricia A Gonzales
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Am Soc Nephrol 20:363-79. 2009
    ..The proteomic data are publicly accessible at http://dir.nhlbi.nih.gov/papers/lkem/exosome/...
  32. pmc An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, Proteomics Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 6:3501-8. 2007
    ..Using a label-free quantification approach, we identified phosphoproteins that changed in abundance with vasopressin exposure including aquaporin-2 (AQP2), Hnrpa3, IP3 receptor 3, and pur-beta...
  33. ncbi request reprint Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSn
    Guanghui Wang
    Proteomics Core Facility and Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Chem 78:5752-61. 2006
    ..Compared with a widely used commercial software tool, QUIL showed improvement in ion chromatogram construction and peak integration and significantly reduced relative errors in abundance ratio assessment...
  34. pmc LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat
    Aaron N Sachs
    National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 295:F1799-806. 2008
    ....
  35. ncbi request reprint Sodium transporter abundance profiling in kidney: effect of spironolactone
    Jakob Nielsen
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Renal Physiol 283:F923-33. 2002
    ....
  36. ncbi request reprint Non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting duct
    Chung Lin Chou
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 279:49026-35. 2004
    ....
  37. pmc Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct
    Maria Barile
    Laboratory of Kidney and Electrolyte Metabolism and Proteomics Core Facility, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 4:1095-106. 2005
    ..These results show that AQP2-containing vesicles are heterogeneous and that intracellular AQP2 resides chiefly in endosomes, trans-Golgi network, and rough endoplasmic reticulum...
  38. pmc Quantitative proteomics identifies vasopressin-responsive nuclear proteins in collecting duct cells
    Laura K Schenk
    National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 23:1008-18. 2012
    ..The findings provide a new online database resource for nuclear proteomics (http://helixweb.nih.gov/ESBL/Database/mNPD/) and generate new hypotheses regarding vasopressin-mediated transcriptional regulation in the collecting duct...
  39. pmc Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct
    Ming Jiun Yu
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:2441-6. 2009
    ..The results implicate ETS family TRs in cell-specific expression of AQP2 and point to HOX, RXR, CREB and GATA family TRs as playing likely additional roles...
  40. pmc Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MS
    Dmitry Tchapyjnikov
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Physiol Genomics 40:167-83. 2010
    ..The newly identified proteins have been incorporated into the IMCD Proteome Database (http://dir.nhlbi.nih.gov/papers/lkem/imcd/)...
  41. pmc Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomics
    Jason D Hoffert
    Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 11:M111.014613. 2012
    ..Finally, we provide an online resource of physiologically regulated phosphorylation sites with dynamic quantitative data (http://helixweb.nih.gov/ESBL/Database/TiPD/index.html)...
  42. pmc Calmodulin is required for vasopressin-stimulated increase in cyclic AMP production in inner medullary collecting duct
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:13624-30. 2005
    ..These studies demonstrate the importance of calmodulin in the regulation of collecting duct adenylyl cyclase activity and transport function...
  43. pmc PhosphoScore: an open-source phosphorylation site assignment tool for MSn data
    Brian E Ruttenberg
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 7:3054-9. 2008
    ..PhosphoScore produced >95% correct MS(2) assignments from known synthetic data, > 98% agreement with an established MS(2) assignment algorithm (Ascore), and >92% agreement with visual inspection of MS(3) and MS(4) spectra...
  44. pmc A selective EP4 PGE2 receptor agonist alleviates disease in a new mouse model of X-linked nephrogenic diabetes insipidus
    Jian Hua Li
    Molecular Signaling Section, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA
    J Clin Invest 119:3115-26. 2009
    ..These findings illustrate the usefulness of the newly generated V2R mutant mice for elucidating and testing new strategies for the potential treatment of humans with XNDI...
  45. ncbi request reprint Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro rat
    Bas W M van Balkom
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 286:F216-24. 2004
    ....
  46. doi request reprint CPhos: a program to calculate and visualize evolutionarily conserved functional phosphorylation sites
    Boyang Zhao
    National Heart, Lung, and Blood Institute, Epithelial Systems Biology Laboratory, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Proteomics 12:3299-303. 2012
    ..CPhos can be accessed or downloaded at http://helixweb.nih.gov/CPhos/...
  47. pmc An online tool for calculation of free-energy balance for the renal inner medulla
    Ryan L Vilbig
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 303:F366-72. 2012
    ..Its use is illustrated by calculating external free-energy balance for an example of the passive concentrating model taken from the original paper by Kokko and Rector (Kokko JP, Rector FC Jr. Kidney Int 2: 214-223, 1972)...
  48. doi request reprint Isolation and purification of exosomes in urine
    Patricia A Gonzales
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 641:89-99. 2010
    ..Validation methods include western blots of pan-exosome markers and segment-specific exosome markers, and negative staining electron microscopy...
  49. pmc Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry
    Nicholas A Zwang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 8:1540-54. 2009
    ..These results suggest that phosphorylation of AQP2 at Ser-256 may regulate AQP2 trafficking in part by mediating differential binding of hsp70 family proteins to the COOH-terminal tail...
  50. pmc High-throughput identification of IMCD proteins using LC-MS/MS
    Trairak Pisitkun
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Physiol Genomics 25:263-76. 2006
    ..A 28-protein vasopressin signaling network was constructed using literature-based network analysis software focusing on the newly identified proteins, providing several new hypotheses for future studies...
  51. ncbi request reprint Hypokalemia in a mouse model of Gitelman's syndrome
    Ryan G Morris
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10, Rm 6N260, 10 Center Dr MSC 1603, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 290:F1416-20. 2006
    ..Therefore, NCC (-/-) mice are more sensitive to reductions in dietary potassium than wild-type mice and become hypokalemic, thus more faithfully representing the Gitelman phenotype seen in humans...
  52. pmc Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites
    Jason D Hoffert
    National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:7159-64. 2006
    ....
  53. pmc Renal phenotype of UT-A urea transporter knockout mice
    Robert A Fenton
    Laboratory of Kidney Electrolyte Metabolism, National Heart, Lung and Blood Institutes, National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 16:1583-92. 2005
    ..These results support our conclusion that the urinary concentrating defect in UT-A1/3(-/-) mice is caused by a failure of urea transport from the IMCD lumen to the inner medullary interstitium, resulting in osmotic diuresis...
  54. ncbi request reprint Identification and quantification of basic and acidic proteins using solution-based two-dimensional protein fractionation and label-free or 18O-labeling mass spectrometry
    Wells W Wu
    Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 6:2447-59. 2007
    ..With the use of HCT116 colon carcinoma cells, a total of 305 basic and 183 acidic proteins was identified. Quantitative proteomics revealed that 17 of these proteins were differentially expressed in HCT116 p53-/- cells...
  55. pmc Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct
    Chung Lin Chou
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 295:F192-201. 2008
    ..We conclude that the AVP-induced cell height increase is dependent on basolateral solute uptake via NKCC1 and changes in actin organization via myosin II, but is not dependent specifically on increased apical water entry...
  56. ncbi request reprint Altered expression profile of transporters in the inner medullary collecting duct of aquaporin-1 knockout mice
    Ryan G Morris
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 289:F194-9. 2005
    ..WT controls. In conclusion, the expression profile of IMCD transporters is markedly altered in AQP1 -/- mice and this manifestation is related to the associated concentrating defect...
  57. pmc Transcriptional profiling of native inner medullary collecting duct cells from rat kidney
    Panapat Uawithya
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland
    Physiol Genomics 32:229-53. 2008
    ..Altogether, the results combine with proteomics studies of the IMCD to provide a framework for modeling complex interaction networks responsible for vasopressin action in collecting duct cells...
  58. pmc Quantitative phosphoproteomics in nuclei of vasopressin-sensitive renal collecting duct cells
    Steven J Bolger
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Cell Physiol 303:C1006-20. 2012
    ..All data are available to users at http://helixweb.nih.gov/ESBL/Database/mNPPD/...
  59. ncbi request reprint The application of DIGE-based proteomics to renal physiology
    Ewout J Hoorn
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nephron Physiol 104:p61-72. 2006
    ..Finally, we illustrate how quantification based on the DIGE approach combined with bioinformatics may facilitate the study of systems biology of the kidney...
  60. pmc Magnetic resonance imaging of urea transporter knockout mice shows renal pelvic abnormalities
    Vinitha A Jacob
    Laboratory of Kidney and Electrolyte Metabolism, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Kidney Int 74:1202-8. 2008
    ..Our studies show that real time imaging of renal pelvic structure in genetically manipulated mice provides a tool for the non-destructive, temporal evaluation of kidney structure...
  61. ncbi request reprint Decreased abundance of collecting duct urea transporters UT-A1 and UT-A3 with ECF volume expansion
    Xiao Yan Wang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Renal Physiol 282:F577-84. 2002
    ..The results support the view that ECF-related changes in serum urea concentration are mediated, at least in part, through altered urea transporter abundance...
  62. pmc Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans
    Panpan Yu
    Developmental Neurobiology Section, Cell Biology and Physiology Center, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e59285. 2013
    ....
  63. doi request reprint Proteomic approaches for the study of cell signaling in the renal collecting duct
    Ewout J Hoorn
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Contrib Nephrol 160:172-85. 2008
    ..We also emphasize that proteomics of body fluids will be the strategy to identify disease biomarkers, and therefore conclude the review by highlighting the perspectives of biomarker discovery in urinary exosomes...
  64. pmc Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting duct
    Robert A Fenton
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
    Proc Natl Acad Sci U S A 101:7469-74. 2004
    ..Thus, these results do not corroborate the predictions of passive medullary concentrating models stating that NaCl accumulation in the inner medulla depends on rapid vasopressin-regulated urea transport across the IMCD epithelium...
  65. ncbi request reprint Prospects for urinary proteomics: exosomes as a source of urinary biomarkers
    Ewout J Hoorn
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Nephrology (Carlton) 10:283-90. 2005
    ..Finally, potential barriers that need to be overcome before urinary proteomics can be applied clinically, are emphasized...
  66. ncbi request reprint Application of difference gel electrophoresis to the identification of inner medullary collecting duct proteins
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Mechanism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10, Rm 6N260, MSC 1603, 10 Center Drive, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 286:F170-9. 2004
    ..These studies document the applicability of a standardized approach to purification of IMCD cells for proteomic analysis of IMCD proteins and demonstrate the feasibility of large scale identification of proteins in the native IMCD cell...
  67. pmc Gene expression databases for kidney epithelial cells
    Jennifer C Huling
    National Institutes of Health 10 Center Dr Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 302:F401-7. 2012
    ..Here, we provide a roadmap to these databases and illustrate how they may be useful in the design and interpretation of physiological studies. The databases can be accessed through http://helixweb.nih.gov/ESBL/Database...
  68. pmc Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule
    Marina Feric
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Cell Physiol 300:C755-70. 2011
    ..An online database from this study (http://dir.nhlbi.nih.gov/papers/lkem/rcmpd/) provides a resource for future studies of transporter regulation...
  69. ncbi request reprint Tandem mass spectrometry in physiology
    Trairak Pisitkun
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Physiology (Bethesda) 22:390-400. 2007
    ..Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology...
  70. pmc Enrichment of distinct microfilament-associated and GTP-binding-proteins in membrane/microvilli fractions from lymphoid cells
    Jian Jiang Hao
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 7:2911-27. 2008
    ....
  71. pmc Tamm-Horsfall protein and urinary exosome isolation
    Patrícia Fernández-Llama
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Kidney Int 77:736-42. 2010
    ....
  72. ncbi request reprint In vacuo isotope coded alkylation technique (IVICAT); an N-terminal stable isotopic label for quantitative liquid chromatography/mass spectrometry proteomics
    Brigitte L Simons
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Rapid Commun Mass Spectrom 20:2463-77. 2006
    ..IVICAT labeling, when used in conjunction with the new proteomics software QUIL, can accurately report relative protein abundances and increase the sequence coverage of proteins of tissue proteomes...
  73. ncbi request reprint cDNA array identification of genes regulated in rat renal medulla in response to vasopressin infusion
    Heddwen L Brooks
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Renal Physiol 284:F218-28. 2003
    ..These studies have identified several transcripts whose abundances are regulated in the inner medulla in response to infusion of dDAVP and that could play roles in the regulation of salt and water excretion...
  74. pmc Large-scale phosphotyrosine proteomic profiling of rat renal collecting duct epithelium reveals predominance of proteins involved in cell polarity determination
    Boyang Zhao
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Cell Physiol 302:C27-45. 2012
    ..In general, these findings mesh well with evidence that tyrosine phosphorylation plays a key role in epithelial polarity determination...
  75. pmc Phosphoproteomics of vasopressin signaling in the kidney
    Jason D Hoffert
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Expert Rev Proteomics 8:157-63. 2011
    ....
  76. ncbi request reprint Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla
    Valery Grinevich
    Section on Endocrine Physiology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 1862, USA
    Kidney Int 65:54-62. 2004
    ..To test this hypothesis, we examined the effect of lipopolysaccharide (LPS) injection on the expression of V2 receptors and aquaporin-2 in the kidney...
  77. ncbi request reprint Targeted proteomic profiling of renal Na(+) transporter and channel abundances in angiotensin II type 1a receptor knockout mice
    Heddwen L Brooks
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Hypertension 39:470-3. 2002
    ....
  78. ncbi request reprint Structural biology. The atomic architecture of a gas channel
    Mark A Knepper
    Laboratory of Kidney and Electrolyte Metabolism, National Institutes of Health, Bethesda, MD 20892, USA
    Science 305:1573-4. 2004
  79. pmc Taking aim at shotgun phosphoproteomics
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Anal Biochem 375:1-10. 2008
  80. pmc UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct
    Robert A Fenton
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Renal Physiol 290:F188-95. 2006
    ..These results demonstrate that 4.2 kb of the UT-Aalpha promoter is sufficient to drive expression of a heterologous reporter gene in a tissue-specific and cell-specific fashion in transgenic mice...
  81. ncbi request reprint Database for renal collecting duct regulatory and transporter proteins
    John Legato
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda 20892 2690, USA
    Physiol Genomics 13:179-81. 2003
    ..Extension of the database is dynamic and is done through a maintenance interface. This permits creation of new categories, updating of existing entries, and addition of new ones...
  82. pmc Aquaporin-2 in the "-omics" era
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 284:14683-7. 2009
    ..Here we review this progress...
  83. pmc Identification and proteomic profiling of exosomes in human urine
    Trairak Pisitkun
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Proc Natl Acad Sci U S A 101:13368-73. 2004
    ..The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine...
  84. pmc Effect of peristaltic contractions of the renal pelvic wall on solute concentrations of the renal inner medulla in the hamster
    Mary Ella C Pruitt
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Renal Physiol 290:F892-6. 2006
    ..This procedure also significantly decreased inner medullary osmolality. We conclude that elimination of the contractions of the renal pelvic wall in the hamster significantly impairs inner medullary concentrating ability...
  85. pmc Combined proteomics and pathways analysis of collecting duct reveals a protein regulatory network activated in vasopressin escape
    Ewout J Hoorn
    National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892, USA
    J Am Soc Nephrol 16:2852-63. 2005
    ..The results demonstrate a dominant protein regulatory network in IMCD cells that is altered in association with vasopressin escape, providing a new framework for further studies of signaling in IMCD...
  86. ncbi request reprint VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influx
    Brij B Singh
    Secretory Physiology Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
    Mol Cell 15:635-46. 2004
    ..In aggregate, these data suggest that VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to carbachol-stimulation of Ca2+ influx...
  87. ncbi request reprint Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction
    Anja M Jensen
    The Water and Salt Research Center, Univ of Aarhus, Institute of Clinical Medicine Dept of Clinical Physiology, Aarhus Univ Hospital, Brendstrupgaardsvej, DK 8200 Aarhus N, Denmark
    Am J Physiol Renal Physiol 291:F1021-32. 2006
    ..In conclusion, candesartan prevents dysregulation of AQP2, sodium transporters, and development of polyuria seen in BUO. This strongly supports the view that candesartan protects kidney function in response to urinary tract obstruction...
  88. ncbi request reprint Lithium-induced NDI in rats is associated with loss of alpha-ENaC regulation by aldosterone in CCD
    Jakob Nielsen
    The Water and Salt Research Center, Institute of Anatomy Bldg 233 Univ of Aarhus, DK 8000 Aarhus, Denmark
    Am J Physiol Renal Physiol 290:F1222-33. 2006
    ....
  89. ncbi request reprint Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats
    Tae Hwan Kwon
    The Water and Salt Research Ctr, Bldg 233 234, Univ of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 288:F673-84. 2005
    ..These results suggest that ANG II AT(1) receptor activation plays a significant role in regulating aquaporin and sodium transporter expression and modulating urine concentration in vivo...
  90. ncbi request reprint COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction
    Rikke Nørregaard
    The Water and Salt Research Center, University of Aarhus, Denmark
    Am J Physiol Renal Physiol 289:F322-33. 2005
    ..These data indicate that COX-2 may be an important factor contributing to the impaired renal water and sodium handling in response to BUO...
  91. ncbi request reprint Oxytocin induces apical and basolateral redistribution of aquaporin-2 in rat kidney
    Un Sil Jeon
    Departmentof Internal Medicine, Gyeong Sang National University, Chinju, Korea
    Nephron Exp Nephrol 93:e36-45. 2003
    ..Oxytocin may be one of the factors that accounts for vasopressin-independent AQP2 targeting in the kidney...
  92. ncbi request reprint Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction
    Chunling Li
    The Water and Salt Research Center and Department of Cell Biology, Institute of Anatomy, University of Aarhus, Denmark
    Am J Physiol Renal Physiol 284:F155-66. 2003
    ....
  93. ncbi request reprint Altered expression of renal NHE3, TSC, BSC-1, and ENaC subunits in potassium-depleted rats
    Marie Louise Elkjaer
    The Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 283:F1376-88. 2002
    ..Downregulation of BSC-1, TSC, and ENaC may contribute to the urinary concentrating defect, whereas upregulation of NHE3 may be compensatory to prevent urinary Na+ loss and/or to maintain intracellular pH levels...
  94. ncbi request reprint Increased apical targeting of renal epithelial sodium channel subunits and decreased expression of type 2 11beta-hydroxysteroid dehydrogenase in rats with CCl4-induced decompensated liver cirrhosis
    Soo Wan Kim
    The Water and Salt Research Center, University of Aarhus, Aarhus, Denmark DK 8000
    J Am Soc Nephrol 16:3196-210. 2005
    ..In conclusion, increased apical targeting of ENaC subunits combined with diminished abundance of 11betaHSD2 in the DCT2, CNT, and cortical collecting duct is likely to play a role in the sodium retaining stage of liver cirrhosis...
  95. ncbi request reprint Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice
    Ming Lu
    Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520 8026, USA
    J Biol Chem 277:37881-7. 2002
    ..Despite loss of ROMK expression, the normokalemic null mice exhibited significantly increased kaliuresis, indicating alternative mechanisms for K(+) absorption/secretion in the nephron...
  96. ncbi request reprint Dysregulation of renal salt and water transport proteins in diabetic Zucker rats
    Crystal A Bickel
    Division of Endocrinology and Metabolism, Department of Medicine, Georgetown University, Washington DC 20007, USA
    Kidney Int 61:2099-110. 2002
    ..The molecular mechanisms involved and how the kidney responds to this volume expansion, in terms of renal transporter regulation, are not understood...
  97. ncbi request reprint Increased apical targeting of renal ENaC subunits and decreased expression of 11betaHSD2 in HgCl2-induced nephrotic syndrome in rats
    Soo Wan Kim
    The Water and Salt Research Center, Bldg 233 234, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 290:F674-87. 2006
    ..The decreased abundance of NHE3, NKCC2, NCC, and Na-K-ATPase may play a compensatory role in promoting sodium excretion...
  98. ncbi request reprint alpha-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction
    Chunling Li
    The Water and Salt Research Center, Institute of Clinical Medicine, University of Aarhus, Brendstrupgaardsvej, DK 8230 Aarhus N, Denmark
    Am J Physiol Renal Physiol 290:F384-96. 2006
    ....
  99. ncbi request reprint Hypotension in NKCC1 null mice: role of the kidneys
    Susan M Wall
    Renal Division, Emory Univ School of Medicine, Atlanta, GA 30322, USA
    Am J Physiol Renal Physiol 290:F409-16. 2006
    ..Increased Na+ transporter expression, increased plasma renin, and reduced plasma ANP, as observed in NKCC1 null mice, should increase vascular volume and blood pressure, thus minimizing hypotension...
  100. ncbi request reprint Expression of urea transporters in the developing rat kidney
    Young Hee Kim
    Department of Anatomy, Catholic University Medical College, Seoul, Korea, 137 701, Georgia 30322, USA
    Am J Physiol Renal Physiol 282:F530-40. 2002
    ..We conclude that the expression of urea transporters in short-loop DTL and DVR coincides with the development of the ability to produce a concentrated urine...
  101. ncbi request reprint Reduced expression of Na-K-2Cl cotransporter in medullary TAL in vitamin D-induced hypercalcemia in rats
    Weidong Wang
    Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 282:F34-44. 2002
    ....