Research Topics
| Margaret C KlineSummaryAffiliation: National Institute of Standards and Technology Country: USA Publications
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Detail Information
Publications
Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival mediaMargaret C Kline
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Anal Chem 74:1863-9. 2002..All of the evaluated media adequately "bank" forensically useful DNA in well-dried whole blood stains for at least 19 months at ambient temperature...
Production and certification of NIST Standard Reference Material 2372 Human DNA Quantitation StandardMargaret C Kline
Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899 8311, USA
Anal Bioanal Chem 394:1183-92. 2009....
STR sequence analysis for characterizing normal, variant, and null allelesMargaret C Kline
National Institute of Standards and Technology, 100 Bureau Drive, M S 8312, Gaithersburg, MD 20899, USA
Forensic Sci Int Genet 5:329-32. 2011..The PCR primer sequences and strategy used for our STR allele sequencing work involving 23 autosomal STRs and 17 Y-chromosome STRs are described along with the results from 111 variant and 17 null alleles...
Results from the NIST 2004 DNA Quantitation StudyMargaret C Kline
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:570-8. 2005..Information from this interlaboratory study is guiding development of a future NIST Standard Reference Material for Human DNA Quantitation, SRM 2372...
Mitochondrial DNA typing screens with control region and coding region SNPsMargaret C Kline
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:377-85. 2005..These types of screening assays should enable more rapid evaluation of forensic casework samples such that only samples not excluded would be subjected to further characterization through full HVI/HVII mtDNA sequence analysis...
High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assaysRichard Schoske
Biotechnology Division, National Institute of Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Forensic Sci Int 139:107-21. 2004..Samples containing the most common type in the European database could be well resolved with additional markers beyond the minimal haplotype loci...
Analysis of mutations in father-son pairs with 17 Y-STR lociAmy E Decker
Biochemical Science Division, National Institute of Sciences and Technology, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int Genet 2:e31-5. 2008..Furthermore, two different sample pairs were found to have two mutations. An African American sample pair had a mutation at DYS458 and a second at DYS635 and an Asian sample pair had mutations at DYS439 and Y-GATA-H4...
NIST mixed stain study 3: signal intensity balance in commercial short tandem repeat multiplexesDavid L Duewer
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8394, USA
Anal Chem 76:6928-34. 2004....
Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samplesJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int 156:250-60. 2006..Allele frequencies for each locus are reported along with nomenclature based on sequence analysis...
Characterization of 26 miniSTR loci for improved analysis of degraded DNA samplesCarolyn R Hill
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 53:73-80. 2008....
Concordance and population studies along with stutter and peak height ratio analysis for the PowerPlex ® ESX 17 and ESI 17 SystemsCarolyn R Hill
National Institute of Standards and Technology, Chemical Science and Technology Laboratory, Gaithersburg, MD 20899 8312, USA
Forensic Sci Int Genet 5:269-75. 2011....
Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kitsCarolyn R Hill
National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
J Forensic Sci 52:870-3. 2007..DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift...
Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populationsJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA
J Forensic Sci 48:908-11. 2003
Chromosomal duplications along the Y-chromosome and their potential impact on Y-STR interpretationJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:853-9. 2005..In addition, duplicated loci typically possess alleles that differ by only a single repeat unit and possess similar peak heights...
A novel multiplex for simultaneous amplification of 20 Y chromosome STR markersJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Forensic Sci Int 129:10-24. 2002..Haplotype comparisons between the 20plex and a commercially available kit found excellent agreement across the 76 samples in the Y chromosome consortium panel...
NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensityMargaret C Kline
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Anal Chem 75:2463-9. 2003..However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems...
