Alan R Kimmel

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Perilipin family members preferentially sequester to either triacylglycerol-specific or cholesteryl-ester-specific intracellular lipid storage droplets
    Kai Hsieh
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 125:4067-76. 2012
  2. doi request reprint An orphan nuclear receptor finds a home
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Mol Cell 37:155-7. 2010
  3. ncbi request reprint Breaking symmetries: regulation of Dictyostelium development through chemoattractant and morphogen signal-response
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    Curr Opin Genet Dev 14:540-9. 2004
  4. pmc Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    J Lipid Res 51:468-71. 2010
  5. pmc A G alpha-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement
    Joseph A Brzostowski
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive Kidney Diseases, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 18:805-15. 2004
  6. pmc Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation
    Carole Sztalryd
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 2715, USA
    J Cell Biol 161:1093-103. 2003
  7. ncbi request reprint Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes
    Chun li Su
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    J Biol Chem 278:43615-9. 2003
  8. doi request reprint Biochemical responses to chemoattractants in Dictyostelium: ligand-receptor interactions and downstream kinase activation
    Xin Hua Liao
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA
    Methods Mol Biol 571:271-81. 2009
  9. pmc Combinatorial cell-specific regulation of GSK3 directs cell differentiation and polarity in Dictyostelium
    Leung Kim
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Development 138:421-30. 2011
  10. ncbi request reprint Structure of a lipid droplet protein; the PAT family member TIP47
    Sabrina J Hickenbottom
    Laboratory of Cellular and Developmental Biology, U S Department of Health and Human Services, Bethesda, MD 20892, USA
    Structure 12:1199-207. 2004

Collaborators

Detail Information

Publications36

  1. pmc Perilipin family members preferentially sequester to either triacylglycerol-specific or cholesteryl-ester-specific intracellular lipid storage droplets
    Kai Hsieh
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 125:4067-76. 2012
    ..Our data suggest diversity of Plin function, alter previous assumptions about shared collective actions of the Plins, and indicate that each Plin can have separate and unique functions...
  2. doi request reprint An orphan nuclear receptor finds a home
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Mol Cell 37:155-7. 2010
    ....
  3. ncbi request reprint Breaking symmetries: regulation of Dictyostelium development through chemoattractant and morphogen signal-response
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    Curr Opin Genet Dev 14:540-9. 2004
    ....
  4. pmc Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    J Lipid Res 51:468-71. 2010
    ..Each gene member will incorporate the root term PERILIPIN (PLIN), the founding gene of the PAT family, with the different genes/proteins numbered sequentially...
  5. pmc A G alpha-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement
    Joseph A Brzostowski
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive Kidney Diseases, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 18:805-15. 2004
    ..Potentially, functionally similar Galpha-mediated inhibitory signaling may exist in other eukaryotic cells to regulate chemoattractant response...
  6. pmc Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation
    Carole Sztalryd
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 2715, USA
    J Cell Biol 161:1093-103. 2003
    ....
  7. ncbi request reprint Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes
    Chun li Su
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    J Biol Chem 278:43615-9. 2003
    ..Thus, HSL translocation requires the phosphorylation of both HSL and perilipin...
  8. doi request reprint Biochemical responses to chemoattractants in Dictyostelium: ligand-receptor interactions and downstream kinase activation
    Xin Hua Liao
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA
    Methods Mol Biol 571:271-81. 2009
    ..e., receptors) per cell. We further present examples for the application of biochemical assays to characterize the ligand-induced kinase activation of PI3K, GSK3, and ERK2...
  9. pmc Combinatorial cell-specific regulation of GSK3 directs cell differentiation and polarity in Dictyostelium
    Leung Kim
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Development 138:421-30. 2011
    ....
  10. ncbi request reprint Structure of a lipid droplet protein; the PAT family member TIP47
    Sabrina J Hickenbottom
    Laboratory of Cellular and Developmental Biology, U S Department of Health and Human Services, Bethesda, MD 20892, USA
    Structure 12:1199-207. 2004
    ..The structure suggests an analogy between PAT proteins and apolipoproteins in which helical repeats interact with lipid while the ordered C-terminal region is involved in protein:protein interactions...
  11. pmc TOR complex 2 (TORC2) in Dictyostelium suppresses phagocytic nutrient capture independently of TORC1-mediated nutrient sensing
    Daniel Rosel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    J Cell Sci 125:37-48. 2012
    ..The integrated and balanced regulation of TORC1 and TORC2 might be crucial in Dictyostelium to coordinate growth and energy needs with other essential TOR-regulated processes...
  12. pmc Chemotactic activation of Dictyostelium AGC-family kinases AKT and PKBR1 requires separate but coordinated functions of PDK1 and TORC2
    Xin Hua Liao
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 8028, USA
    J Cell Sci 123:983-92. 2010
    ..Despite certain similarities, AKT and PKBR1 have distinct regulatory paths that impact activation and effector targeting, with PDK1 serving a central role...
  13. pmc Chemoattractant stimulation of TORC2 is regulated by receptor/G protein-targeted inhibitory mechanisms that function upstream and independently of an essential GEF/Ras activation pathway in Dictyostelium
    Xin Hua Liao
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Mol Biol Cell 24:2146-55. 2013
    ..We suggest that ligand adaptation functions in upstream inhibitory pathways that involve chemoattractant-specific receptor/G protein complexes and regulate multiple response pathways. ..
  14. ncbi request reprint Functional studies on native and mutated forms of perilipins. A role in protein kinase A-mediated lipolysis of triacylglycerols
    John T Tansey
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 8028, USA
    J Biol Chem 278:8401-6. 2003
    ..Moreover, the data indicate that the unique C-terminal portion of perilipin A is responsible for its protection against lipolysis and that phosphorylation at the N-terminal PKA sites attenuates this protective effect...
  15. pmc Dictyostelium possesses highly diverged presenilin/gamma-secretase that regulates growth and cell-fate specification and can accurately process human APP: a system for functional studies of the presenilin/gamma-secretase complex
    Vanessa C McMains
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Dis Model Mech 3:581-94. 2010
    ....
  16. pmc Nonadaptive regulation of ERK2 in Dictyostelium: implications for mechanisms of cAMP relay
    Joseph A Brzostowski
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Mol Biol Cell 17:4220-7. 2006
    ..This work redefines mechanisms of ERK2 regulation by 7-TMR signaling in Dictyostelium and establishes new implications for control of signal relay during chemotaxis...
  17. ncbi request reprint Functional conservation for lipid storage droplet association among Perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium
    Shinji Miura
    Membrane Regulation Section, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20992 8028, USA
    J Biol Chem 277:32253-7. 2002
    ....
  18. pmc Different CHD chromatin remodelers are required for expression of distinct gene sets and specific stages during development of Dictyostelium discoideum
    James L Platt
    Laboratory of Cellular and Developmental Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Development 140:4926-36. 2013
    ..This study provides novel insight into the broad function of CHDs in the regulation development and disease, through chromatin-mediated changes in directed gene expression. ..
  19. pmc Dictyostelium discoideum expresses a malaria chloroquine resistance mechanism upon transfection with mutant, but not wild-type, Plasmodium falciparum transporter PfCRT
    Bronwen Naudé
    Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892 8132, USA
    J Biol Chem 280:25596-603. 2005
    ..Transformed D. discoideum will be useful for further studies of the chloroquine resistance mechanism and may assist in the development and evaluation of new antimalarial drugs...
  20. ncbi request reprint The signal to move: D. discoideum go orienteering
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Science 300:1525-7. 2003
    ..Many of the molecular interactions described are fundamental to the regulation of chemotaxis in other eukaryotic cells...
  21. doi request reprint Growth control via TOR kinase signaling, an intracellular sensor of amino acid and energy availability, with crosstalk potential to proline metabolism
    Xin Hua Liao
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Amino Acids 35:761-70. 2008
    ..This review will focus on the regulation of TORC1 in mammalian cells in the context of amino acid sensing/regulation and intracellular ATP homeostasis, but will also include comparisons among other organisms...
  22. ncbi request reprint Galpha-mediated inhibition of developmental signal response
    Joseph A Brzostowski
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Curr Biol 12:1199-208. 2002
    ..We now demonstrate through loss-of-function/gain-of-function studies that the novel heterotrimeric Galpha9 protein subunit regulates an inhibitory pathway during early Dictyostelium development for the cAMP signal response...
  23. pmc A Rab21/LIM-only/CH-LIM complex regulates phagocytosis via both activating and inhibitory mechanisms
    Taruna Khurana
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 2715, USA
    EMBO J 24:2254-64. 2005
    ..Finally, we demonstrate that ChLim and LimF localize to the phagocytic cup and phago-lysosomal vesicles. We suggest that LimF, ChLim, and activated Rab21-GTP participate as a novel signaling complex that regulates phagocytic activity...
  24. ncbi request reprint Teaching resources. Spatial and temporal dynamics of signaling components involved in the control of chemotaxis in Dictyostelium discoideum
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Sci STKE 2004:tr3. 2004
    ....
  25. doi request reprint Oscillatory signaling and network responses during the development of Dictyostelium discoideum
    Vanessa C McMains
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Ageing Res Rev 7:234-48. 2008
    ..In suspension culture, >10(10) cells can oscillate in harmony. This review focuses on molecular mechanisms that cyclically activate and attenuate signal propagation and on chemotactic responses to oscillatory wave progression...
  26. ncbi request reprint Rapid, Wnt-induced changes in GSK3beta associations that regulate beta-catenin stabilization are mediated by Galpha proteins
    Xunxian Liu
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:1989-97. 2005
    ..Yet, the mechanisms that underlie Wnt regulation of GSK3 and stabilization of beta-catenin are still not fully appreciated...
  27. ncbi request reprint The COP9 signalosome regulates cell proliferation of Dictyostelium discoideum
    Daniel Rosel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Eur J Cell Biol 85:1023-34. 2006
    ..Finally, we speculate on a potential role of CSN in cullin function and regulated protein destruction during multicellular development of Dictyostelium...
  28. ncbi request reprint Receptor-dependent and tyrosine phosphatase-mediated inhibition of GSK3 regulates cell fate choice
    Leung Kim
    Laboratory of Cellular and Developmental Biology, Building 50 3351, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 3:523-32. 2002
    ..CARs differentially regulate GSK3 activity by selectively activating a tyrosine phosphatase or kinase for pattern formation. The findings may provide a comparative understanding of CAR-GSK3 and Wnt/Frizzled-GSK3 pathways...
  29. doi request reprint Perilipin 5, a lipid droplet protein adapted to mitochondrial energy utilization
    Alan R Kimmel
    aLaboratory of Cellular and Developmental Biology 50 3351, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland bThe Geriatric Research, Education and Clinical Center, Baltimore Veterans Affairs Healthcare Center, Division of Endocrinology, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland, USA
    Curr Opin Lipidol 25:110-7. 2014
    ..We summarize recent mechanistic and physiological studies related to the role of perilipin 5 (Plin5) in regulating lipid droplet accumulation and protection to fatty acids in tissues with high lipid oxidative metabolism...
  30. doi request reprint Analysis of chromatin organization by deep sequencing technologies
    James L Platt
    Laboratory of Cellular and Developmental Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 983:173-83. 2013
    ....
  31. ncbi request reprint Generation of multiple knockout mutants using the Cre-loxP system
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 346:187-99. 2006
    ..We have, therefore, developed a robust system and describe a protocol for the generation of multiple gene mutations in Dictyostelium by recycling the Blasticidin S selectable marker after transient expression of Cre recombinase...
  32. pmc dictyBase: a new Dictyostelium discoideum genome database
    Lisa Kreppel
    Laboratory of Cellular and Developmental Biology 50 3351, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Nucleic Acids Res 32:D332-3. 2004
    ....
  33. pmc Genomic database resources for Dictyostelium discoideum
    Lisa Kreppel
    Laboratory of Cellular and Developmental Biology 50 3351, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8028, USA
    Nucleic Acids Res 30:84-6. 2002
    ..imb-jena.de/dictyostelium/; http://dictygenome.bcm.tmc.edu/; http://www.sanger. ac.uk/Projects/D_discoideum/; http://www.csm.biol. tsukuba.ac.jp/cDNAproject.html)...
  34. pmc Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling
    Paul B Bokko
    Department of Microbiology, La Trobe University, Melbourne, Victoria 3086, Australia
    Mol Biol Cell 18:1874-86. 2007
    ..The results show that diverse cytopathologies in Dictyostelium mitochondrial disease are caused by chronic AMPK signaling not by insufficient ATP...
  35. ncbi request reprint GSK3 at the edge: regulation of developmental specification and cell polarization
    Leung Kim
    Department of Biological Sciences, Florida International University, Miami, FL 33199, USA
    Curr Drug Targets 7:1411-9. 2006
    ..We discuss the central role of GSK3 in control of the Wnt, Hedgehog, cAMP (in Dictyostelium), and other signaling pathways, but also focus on significant new evidence that GSK3 is required to establish cell polarity...
  36. pmc A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system
    Jan Faix
    A Butenandt Institut Zellbiologie, Ludwig Maximilians Universitat Munchen, D 80336 MUnchen, Germany
    Nucleic Acids Res 32:e143. 2004
    ..Furthermore, the cells remain sensitive to transformation for additional targeted or random mutagenesis requiring Blasticidin selection and for functional expression studies of mutated or tagged proteins using other selectable markers...