J J Kasianowicz
Affiliation: National Institute of Standards and Technology
- Nanometer-scale pores: potential applications for analyte detection and DNA characterizationJohn J Kasianowicz
Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
Dis Markers 18:185-91. 2002..We briefly review here these studies and identify leaps in technology that, if realized, might lead to innovations for the early detection of cancer...
- Detecting and characterizing individual molecules with single nanoporesJohn J Kasianowicz
Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
Methods Mol Biol 870:3-20. 2012..We describe herein some of our methods and approaches to address this issue...
- Nanoscopic porous sensorsJohn J Kasianowicz
National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
Annu Rev Anal Chem (Palo Alto Calif) 1:737-66. 2008..In this review we survey this field of research and discuss the prospects for advances that could be made in the near future...
- Enhancing molecular flux through nanopores by means of attractive interactionsJohn J Kasianowicz
Electrical and Electronic Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA
Proc Natl Acad Sci U S A 103:11431-2. 2006
- Genetically engineered metal ion binding sites on the outside of a Channel's transmembrane beta-barrelJ J Kasianowicz
National Institute of Standards and Technology, Biotechnology Division, Gaithersburg, Maryland 20899, USA
Biophys J 76:837-45. 1999..Thus, analyte-induced current blockades alone might not establish whether an analyte binding site is inside a pore...
- Simultaneous multianalyte detection with a nanometer-scale poreJ J Kasianowicz
NIST, Biotechnology Division, Gaithersburg, Maryland 20899 8313, USA
Anal Chem 73:2268-72. 2001..We demonstrate here that two different proteins can be simultaneously detected with this technique...
- Frequency response of alternating currents through the Staphylococcus aureus alpha-hemolysin ion channelM Misakian
NIST, Electricity Division, Gaithersburg, Maryland 20899 8113, USA
Bioelectromagnetics 22:487-93. 2001..The possible usefulness of frequency response data for ion channels in cell membranes during investigations of biological effects of time-varying magnetic fields is briefly discussed...
- Theory for polymer analysis using nanopore-based single-molecule mass spectrometryJoseph E Reiner
Semiconductor Electronics Division, Electronics and Electrical Engineering Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899 8120, USA
Proc Natl Acad Sci U S A 107:12080-5. 2010..76 meV per ethylene glycol monomer). The results suggest that rational, physical models for the analysis of analyte-nanopore interactions will develop the full potential of nanopore-based sensing for chemical and biological applications...
- Sizing the Bacillus anthracis PA63 channel with nonelectrolyte poly(ethylene glycols)Brian J Nablo
Electrical and Electronics Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
Biophys J 95:1157-64. 2008..The results suggest that the limiting diameter of the PA(63) pore is <2 nm, which is consistent with an all-atom model of the PA(63) channel and previous experiments using large ions...
- Electrostatic influence on ion transport through the alphaHL channelM Misakian
Biotechnology Division, National Institute of Standards and Technology, ACSL 227/A251, Gaithersburg, MD 20899-8313, USA
J Membr Biol 195:137-46. 2003..The model demonstrates that rectification of monovalent ion flow through the alphaHL channel can be related to the asymmetry in the location of the ionizable amino acid side chains...
- Probing single nanometer-scale pores with polymeric molecular rulersSarah E Henrickson
Semiconductor Electronics Division, NIST, Bldg 225, Room B326, Gaithersburg, Maryland 20899 8120, USA
J Chem Phys 132:135101. 2010..The results are consistent with the channel crystal structure and suggest that polymer-based "molecular rulers" may prove useful in deducing the structures of nanometer-scale pores in general...
- Single-molecule mass spectrometry in solution using a solitary nanoporeJoseph W F Robertson
Electronics and Electrical Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8120, USA
Proc Natl Acad Sci U S A 104:8207-11. 2007..This technique could prove useful for the real-time characterization of molecules in solution...
- Anthrax biosensor, protective antigen ion channel asymmetric blockadeKelly M Halverson
United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA
J Biol Chem 280:34056-62. 2005..The latter two results suggest the potential application of PA63 nanopore-based biosensors in anthrax therapeutics and diagnostics...
- Functional reconstitution of protein ion channels into planar polymerizable phospholipid membranesDevanand K Shenoy
Center for Bio Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA
Nano Lett 5:1181-5. 2005..The ability to stabilize nanoscale pores in robust ultrathin films may prove useful in single molecule sensing applications...
- Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chipLouis P Hromada
University of Maryland, College Park, Department of Mechanical Engineering, College Park, Maryland 20742, USA
Lab Chip 8:602-8. 2008..Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented...
- Membrane surface dynamics of DNA-threaded nanopores revealed by simultaneous single-molecule optical and ensemble electrical recordingEmily L Chandler
Department of Chemistry, Wheaton College, Wheaton, Illinois 60187, USA
Langmuir 20:898-905. 2004....
- Nanopores: flossing with DNAJohn J Kasianowicz
Nat Mater 3:355-6. 2004
- Effects of cytochrome c on the mitochondrial apoptosis-induced channel MACLiang Guo
Dept. of Basic Sciences, College of Dentistry, New York Univ, 345 East 24th St, New York, NY 10010, USA
Am J Physiol Cell Physiol 286:C1109-17. 2004..These findings are consistent with the notion that MAC is the pore through which cytochrome c is released from mitochondria during apoptosis...
- Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesisPetr G Merzlyak
Laboratory of Membrane Biophysics, Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil
Biophys J 89:3059-70. 2005....