J J Kasianowicz

Summary

Affiliation: National Institute of Standards and Technology
Country: USA

Publications

  1. ncbi request reprint Nanometer-scale pores: potential applications for analyte detection and DNA characterization
    John J Kasianowicz
    Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
    Dis Markers 18:185-91. 2002
  2. doi request reprint Detecting and characterizing individual molecules with single nanopores
    John J Kasianowicz
    Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Methods Mol Biol 870:3-20. 2012
  3. doi request reprint Nanoscopic porous sensors
    John J Kasianowicz
    National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
    Annu Rev Anal Chem (Palo Alto Calif) 1:737-66. 2008
  4. pmc Enhancing molecular flux through nanopores by means of attractive interactions
    John J Kasianowicz
    Electrical and Electronic Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8120, USA
    Proc Natl Acad Sci U S A 103:11431-2. 2006
  5. pmc Genetically engineered metal ion binding sites on the outside of a Channel's transmembrane beta-barrel
    J J Kasianowicz
    National Institute of Standards and Technology, Biotechnology Division, Gaithersburg, Maryland 20899, USA
    Biophys J 76:837-45. 1999
  6. ncbi request reprint Simultaneous multianalyte detection with a nanometer-scale pore
    J J Kasianowicz
    NIST, Biotechnology Division, Gaithersburg, Maryland 20899 8313, USA
    Anal Chem 73:2268-72. 2001
  7. ncbi request reprint Frequency response of alternating currents through the Staphylococcus aureus alpha-hemolysin ion channel
    M Misakian
    NIST, Electricity Division, Gaithersburg, Maryland 20899 8113, USA
    Bioelectromagnetics 22:487-93. 2001
  8. pmc Theory for polymer analysis using nanopore-based single-molecule mass spectrometry
    Joseph E Reiner
    Semiconductor Electronics Division, Electronics and Electrical Engineering Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899 8120, USA
    Proc Natl Acad Sci U S A 107:12080-5. 2010
  9. pmc Sizing the Bacillus anthracis PA63 channel with nonelectrolyte poly(ethylene glycols)
    Brian J Nablo
    Electrical and Electronics Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
    Biophys J 95:1157-64. 2008
  10. ncbi request reprint Electrostatic influence on ion transport through the alphaHL channel
    M Misakian
    Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
    J Membr Biol 195:137-46. 2003

Collaborators

  • Joseph E Reiner
  • M Misakian
  • H H Weetall
  • H Bayley
  • Qian Wang
  • R G Panchal
  • Devanand K Shenoy
  • Brian J Nablo
  • Joseph W F Robertson
  • Oleg V Krasilnikov
  • Kelly M Halverson
  • Sarah E Henrickson
  • Vincent M Stanford
  • Louis P Hromada
  • Rick Gussio
  • Sina Bavari
  • Tam L Nguyen
  • Petr G Merzlyak
  • Liang Guo
  • Emily L Chandler
  • Edmund A DiMarzio
  • Michael A Gaitan
  • Don L DeVoe
  • Kenneth A Rubinson
  • Claudio G Rodrigues
  • Stephen F Little
  • Maria Fatima P Capistrano
  • Angela Valeva
  • Lisa M Burden
  • Evgeny V Pavlov
  • Daniel L Burden
  • Stanley J Korsmeyer
  • Alyssa L Smith
  • Dawn Pietkiewicz
  • Sergey M Grigoriev
  • Bruno Antonsson
  • Kathleen W Kinnally
  • Laurent M Dejean

Detail Information

Publications19

  1. ncbi request reprint Nanometer-scale pores: potential applications for analyte detection and DNA characterization
    John J Kasianowicz
    Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
    Dis Markers 18:185-91. 2002
    ..We briefly review here these studies and identify leaps in technology that, if realized, might lead to innovations for the early detection of cancer...
  2. doi request reprint Detecting and characterizing individual molecules with single nanopores
    John J Kasianowicz
    Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Methods Mol Biol 870:3-20. 2012
    ..We describe herein some of our methods and approaches to address this issue...
  3. doi request reprint Nanoscopic porous sensors
    John J Kasianowicz
    National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
    Annu Rev Anal Chem (Palo Alto Calif) 1:737-66. 2008
    ..In this review we survey this field of research and discuss the prospects for advances that could be made in the near future...
  4. pmc Enhancing molecular flux through nanopores by means of attractive interactions
    John J Kasianowicz
    Electrical and Electronic Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8120, USA
    Proc Natl Acad Sci U S A 103:11431-2. 2006
  5. pmc Genetically engineered metal ion binding sites on the outside of a Channel's transmembrane beta-barrel
    J J Kasianowicz
    National Institute of Standards and Technology, Biotechnology Division, Gaithersburg, Maryland 20899, USA
    Biophys J 76:837-45. 1999
    ..Thus, analyte-induced current blockades alone might not establish whether an analyte binding site is inside a pore...
  6. ncbi request reprint Simultaneous multianalyte detection with a nanometer-scale pore
    J J Kasianowicz
    NIST, Biotechnology Division, Gaithersburg, Maryland 20899 8313, USA
    Anal Chem 73:2268-72. 2001
    ..We demonstrate here that two different proteins can be simultaneously detected with this technique...
  7. ncbi request reprint Frequency response of alternating currents through the Staphylococcus aureus alpha-hemolysin ion channel
    M Misakian
    NIST, Electricity Division, Gaithersburg, Maryland 20899 8113, USA
    Bioelectromagnetics 22:487-93. 2001
    ..The possible usefulness of frequency response data for ion channels in cell membranes during investigations of biological effects of time-varying magnetic fields is briefly discussed...
  8. pmc Theory for polymer analysis using nanopore-based single-molecule mass spectrometry
    Joseph E Reiner
    Semiconductor Electronics Division, Electronics and Electrical Engineering Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899 8120, USA
    Proc Natl Acad Sci U S A 107:12080-5. 2010
    ..76 meV per ethylene glycol monomer). The results suggest that rational, physical models for the analysis of analyte-nanopore interactions will develop the full potential of nanopore-based sensing for chemical and biological applications...
  9. pmc Sizing the Bacillus anthracis PA63 channel with nonelectrolyte poly(ethylene glycols)
    Brian J Nablo
    Electrical and Electronics Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8120, USA
    Biophys J 95:1157-64. 2008
    ..The results suggest that the limiting diameter of the PA(63) pore is <2 nm, which is consistent with an all-atom model of the PA(63) channel and previous experiments using large ions...
  10. ncbi request reprint Electrostatic influence on ion transport through the alphaHL channel
    M Misakian
    Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
    J Membr Biol 195:137-46. 2003
    ..The model demonstrates that rectification of monovalent ion flow through the alphaHL channel can be related to the asymmetry in the location of the ionizable amino acid side chains...
  11. doi request reprint Probing single nanometer-scale pores with polymeric molecular rulers
    Sarah E Henrickson
    Semiconductor Electronics Division, NIST, Bldg 225, Room B326, Gaithersburg, Maryland 20899 8120, USA
    J Chem Phys 132:135101. 2010
    ..The results are consistent with the channel crystal structure and suggest that polymer-based "molecular rulers" may prove useful in deducing the structures of nanometer-scale pores in general...
  12. pmc Single-molecule mass spectrometry in solution using a solitary nanopore
    Joseph W F Robertson
    Electronics and Electrical Engineering Laboratory, Semiconductor Electronics Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8120, USA
    Proc Natl Acad Sci U S A 104:8207-11. 2007
    ..This technique could prove useful for the real-time characterization of molecules in solution...
  13. ncbi request reprint Anthrax biosensor, protective antigen ion channel asymmetric blockade
    Kelly M Halverson
    United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702 5011, USA
    J Biol Chem 280:34056-62. 2005
    ..The latter two results suggest the potential application of PA63 nanopore-based biosensors in anthrax therapeutics and diagnostics...
  14. ncbi request reprint Functional reconstitution of protein ion channels into planar polymerizable phospholipid membranes
    Devanand K Shenoy
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA
    Nano Lett 5:1181-5. 2005
    ..The ability to stabilize nanoscale pores in robust ultrathin films may prove useful in single molecule sensing applications...
  15. doi request reprint Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip
    Louis P Hromada
    University of Maryland, College Park, Department of Mechanical Engineering, College Park, Maryland 20742, USA
    Lab Chip 8:602-8. 2008
    ..Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented...
  16. ncbi request reprint Membrane surface dynamics of DNA-threaded nanopores revealed by simultaneous single-molecule optical and ensemble electrical recording
    Emily L Chandler
    Department of Chemistry, Wheaton College, Wheaton, Illinois 60187, USA
    Langmuir 20:898-905. 2004
    ....
  17. ncbi request reprint Nanopores: flossing with DNA
    John J Kasianowicz
    Nat Mater 3:355-6. 2004
  18. ncbi request reprint Effects of cytochrome c on the mitochondrial apoptosis-induced channel MAC
    Liang Guo
    Dept of Basic Sciences, College of Dentistry, New York Univ, 345 East 24th St, New York, NY 10010, USA
    Am J Physiol Cell Physiol 286:C1109-17. 2004
    ..These findings are consistent with the notion that MAC is the pore through which cytochrome c is released from mitochondria during apoptosis...
  19. pmc Conductance and ion selectivity of a mesoscopic protein nanopore probed with cysteine scanning mutagenesis
    Petr G Merzlyak
    Laboratory of Membrane Biophysics, Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil
    Biophys J 89:3059-70. 2005
    ....