Research Topics
| Ronald L JohnsonSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
A quantitative high-throughput screen identifies novel inhibitors of the interaction of thyroid receptor beta with a peptide of steroid receptor coactivator 2Ronald L Johnson
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
J Biomol Screen 16:618-27. 2011..Selected compounds were tested as independent samples, and a methylsulfonylnitrobenzoate series inhibited the TRβ-SRC2 interaction with 5 µM IC(50). This series represents a new class of thyroid hormone receptor-coactivator modulators...- A quantitative high-throughput screen for modulators of IL-6 signaling: a model for interrogating biological networks using chemical librariesRonald L Johnson
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biosyst 5:1039-50. 2009..Small molecules within these series will make useful tool compounds to investigate IL-6 signaling mediated by JAK-STAT activation... - A quantitative high-throughput screen identifies potential epigenetic modulators of gene expressionRonald L Johnson
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
Anal Biochem 375:237-48. 2008..These results suggest that the identified small molecules act on epigenetic or transcriptional components and validate our approach of using a cell-based imaging assay in conjunction with qHTS... - Chemical genomic profiling for antimalarial therapies, response signatures, and molecular targetsJing Yuan
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 333:724-9. 2011..Drugs whose responses mapped to wild-type or mutant pfcrt alleles were tested in combination in vitro and in vivo, which yielded promising new leads for antimalarial treatments... 
High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit themChrissie Y Lee
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, United States
Methods 57:234-48. 2012....
High-throughput screening assays for the identification of chemical probesJames Inglese
US National Institutes of Health Chemical Genomics Center, National Institutes of Health, 9800 Medical Center Drive, Bethesda, Maryland 20892 3370, USA
Nat Chem Biol 3:466-79. 2007..We conclude with special considerations for configuring sensitive, robust, informative and economically feasible HTS assays...- Characterization of chemical libraries for luciferase inhibitory activityDouglas S Auld
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
J Med Chem 51:2372-86. 2008..pyralis luciferase. We describe the structure-activity relationship of the luciferase inhibitors and discuss the use of this data in the interpretation of HTS results and configuration of luciferase-based assays... - Fluorescent protein-based cellular assays analyzed by laser-scanning microplate cytometry in 1536-well plate formatDouglas S Auld
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
Methods Enzymol 414:566-89. 2006..This chapter illustrates the application of microplate laser cytometry to these assays in a manner that is suitable for screening large compound collections in high throughput... - The pilot phase of the NIH Chemical Genomics CenterCraig J Thomas
NIH Chemical Genomics Center, NHGRI, National Institutes of Health, 9800 Medical Center Drive, Building B, Room 3005, MSC 3370, Bethesda, MD 20892 3370, USA
Curr Top Med Chem 9:1181-93. 2009.... - Genetic mapping of targets mediating differential chemical phenotypes in Plasmodium falciparumJing Yuan
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
Nat Chem Biol 5:765-71. 2009..This study identifies new leads for antimalarial drugs and demonstrates the utility of a high-throughput chemical genomic strategy for studying malaria traits... - A class of tricyclic compounds blocking malaria parasite oocyst development and transmissionRichard T Eastman
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Antimicrob Agents Chemother 57:425-35. 2013..Further clinical evaluation of ketotifen and related compounds, including synthetic new derivatives, in blocking malaria transmission may provide new weapons for the current effort of malaria eradication... - An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cellsWenge Zhu
National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 2753, USA
Mol Cancer Res 9:294-310. 2011..Thus, this assay provides a new approach to the discovery of compounds useful for investigating the regulation of genome duplication and for the treatment of cancer... - Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical librariesJames Inglese
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892 3370, USA
Proc Natl Acad Sci U S A 103:11473-8. 2006..qHTS produces rich data sets that can be immediately mined for reliable biological activities, thereby providing a platform for chemical genomics and accelerating the identification of leads for drug discovery...