Ronald L Johnson

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc A quantitative high-throughput screen identifies novel inhibitors of the interaction of thyroid receptor beta with a peptide of steroid receptor coactivator 2
    Ronald L Johnson
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
    J Biomol Screen 16:618-27. 2011
  2. pmc A quantitative high-throughput screen for modulators of IL-6 signaling: a model for interrogating biological networks using chemical libraries
    Ronald L Johnson
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biosyst 5:1039-50. 2009
  3. pmc A quantitative high-throughput screen identifies potential epigenetic modulators of gene expression
    Ronald L Johnson
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 375:237-48. 2008
  4. pmc Chemical genomic profiling for antimalarial therapies, response signatures, and molecular targets
    Jing Yuan
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 333:724-9. 2011
  5. doi request reprint High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit them
    Chrissie Y Lee
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, United States
    Methods 57:234-48. 2012
  6. ncbi request reprint High-throughput screening assays for the identification of chemical probes
    James Inglese
    US National Institutes of Health Chemical Genomics Center, National Institutes of Health, 9800 Medical Center Drive, Bethesda, Maryland 20892 3370, USA
    Nat Chem Biol 3:466-79. 2007
  7. doi request reprint Characterization of chemical libraries for luciferase inhibitory activity
    Douglas S Auld
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
    J Med Chem 51:2372-86. 2008
  8. pmc A class of tricyclic compounds blocking malaria parasite oocyst development and transmission
    Richard T Eastman
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Antimicrob Agents Chemother 57:425-35. 2013
  9. ncbi request reprint Fluorescent protein-based cellular assays analyzed by laser-scanning microplate cytometry in 1536-well plate format
    Douglas S Auld
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Methods Enzymol 414:566-89. 2006
  10. pmc The pilot phase of the NIH Chemical Genomics Center
    Craig J Thomas
    NIH Chemical Genomics Center, NHGRI, National Institutes of Health, 9800 Medical Center Drive, Building B, Room 3005, MSC 3370, Bethesda, MD 20892 3370, USA
    Curr Top Med Chem 9:1181-93. 2009

Detail Information

Publications13

  1. pmc A quantitative high-throughput screen identifies novel inhibitors of the interaction of thyroid receptor beta with a peptide of steroid receptor coactivator 2
    Ronald L Johnson
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
    J Biomol Screen 16:618-27. 2011
    ..Selected compounds were tested as independent samples, and a methylsulfonylnitrobenzoate series inhibited the TRβ-SRC2 interaction with 5 µM IC(50). This series represents a new class of thyroid hormone receptor-coactivator modulators...
  2. pmc A quantitative high-throughput screen for modulators of IL-6 signaling: a model for interrogating biological networks using chemical libraries
    Ronald L Johnson
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biosyst 5:1039-50. 2009
    ..Small molecules within these series will make useful tool compounds to investigate IL-6 signaling mediated by JAK-STAT activation...
  3. pmc A quantitative high-throughput screen identifies potential epigenetic modulators of gene expression
    Ronald L Johnson
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 375:237-48. 2008
    ..These results suggest that the identified small molecules act on epigenetic or transcriptional components and validate our approach of using a cell-based imaging assay in conjunction with qHTS...
  4. pmc Chemical genomic profiling for antimalarial therapies, response signatures, and molecular targets
    Jing Yuan
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 333:724-9. 2011
    ..Drugs whose responses mapped to wild-type or mutant pfcrt alleles were tested in combination in vitro and in vivo, which yielded promising new leads for antimalarial treatments...
  5. doi request reprint High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit them
    Chrissie Y Lee
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, United States
    Methods 57:234-48. 2012
    ....
  6. ncbi request reprint High-throughput screening assays for the identification of chemical probes
    James Inglese
    US National Institutes of Health Chemical Genomics Center, National Institutes of Health, 9800 Medical Center Drive, Bethesda, Maryland 20892 3370, USA
    Nat Chem Biol 3:466-79. 2007
    ..We conclude with special considerations for configuring sensitive, robust, informative and economically feasible HTS assays...
  7. doi request reprint Characterization of chemical libraries for luciferase inhibitory activity
    Douglas S Auld
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
    J Med Chem 51:2372-86. 2008
    ..pyralis luciferase. We describe the structure-activity relationship of the luciferase inhibitors and discuss the use of this data in the interpretation of HTS results and configuration of luciferase-based assays...
  8. pmc A class of tricyclic compounds blocking malaria parasite oocyst development and transmission
    Richard T Eastman
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Antimicrob Agents Chemother 57:425-35. 2013
    ..Further clinical evaluation of ketotifen and related compounds, including synthetic new derivatives, in blocking malaria transmission may provide new weapons for the current effort of malaria eradication...
  9. ncbi request reprint Fluorescent protein-based cellular assays analyzed by laser-scanning microplate cytometry in 1536-well plate format
    Douglas S Auld
    NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
    Methods Enzymol 414:566-89. 2006
    ..This chapter illustrates the application of microplate laser cytometry to these assays in a manner that is suitable for screening large compound collections in high throughput...
  10. pmc The pilot phase of the NIH Chemical Genomics Center
    Craig J Thomas
    NIH Chemical Genomics Center, NHGRI, National Institutes of Health, 9800 Medical Center Drive, Building B, Room 3005, MSC 3370, Bethesda, MD 20892 3370, USA
    Curr Top Med Chem 9:1181-93. 2009
    ....
  11. pmc Genetic mapping of targets mediating differential chemical phenotypes in Plasmodium falciparum
    Jing Yuan
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
    Nat Chem Biol 5:765-71. 2009
    ..This study identifies new leads for antimalarial drugs and demonstrates the utility of a high-throughput chemical genomic strategy for studying malaria traits...
  12. pmc An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cells
    Wenge Zhu
    National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 2753, USA
    Mol Cancer Res 9:294-310. 2011
    ..Thus, this assay provides a new approach to the discovery of compounds useful for investigating the regulation of genome duplication and for the treatment of cancer...
  13. pmc Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries
    James Inglese
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892 3370, USA
    Proc Natl Acad Sci U S A 103:11473-8. 2006
    ..qHTS produces rich data sets that can be immediately mined for reliable biological activities, thereby providing a platform for chemical genomics and accelerating the identification of leads for drug discovery...