Research Topics
Genomes and Genes | Tian JinSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensingXuehua Xu
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
J Cell Biol 178:141-53. 2007..Our study demonstrates a novel spatiotemporal feature of currently unknown inhibitory mechanisms acting locally on the PI3K activation pathway...
GPCR-controlled chemotaxis in Dictyostelium discoideumTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook Facility, Rockville, MD, USA
Wiley Interdiscip Rev Syst Biol Med 3:717-27. 2011..We believe that such an approach will lead to a much better understanding of GPCR-controlled chemotaxis of all eukaryotic cells...- Chemotaxis, chemokine receptors and human diseaseTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12441 Parklawn Drive, Rockville, MD 20852, USA
Cytokine 44:1-8. 2008..In this review, we wish to mention landmark discoveries in the chemotaxis research field that shaped our current understanding of this fundamental cell behavior and lay out key questions that remain to be addressed in the future... - How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideumTian Jin
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Immunol Res 43:118-27. 2009..Here, we review recent research in these areas that have been conducted in the Chemotaxis Signal Section of NIAID's Laboratory of Immunogenetics... 
Moving toward understanding eukaryotic chemotaxisTian Jin
Chemotaxis Signal Section, Laboratories of Immunogenetics, NIAID, NIH, Rockville, MD 20852, USA
Eur J Cell Biol 85:905-13. 2006..In this review, we wish to look back at the journey that has led to our current understanding of chemotaxis of eukaryotic cells...- Fluorescence resonance energy transfer imaging reveals that chemokine-binding modulates heterodimers of CXCR4 and CCR5 receptorsNilgun Isik
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, Rockville, MD, USA
PLoS ONE 3:e3424. 2008..Both receptors are recruited to the immunological synapse during T-cell activation. However, it is not clear whether they form heterodimers and whether ligand binding modulates the dimer formation... - A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxisJianshe Yan
Chemotaxis Signal Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Dev Cell 22:92-103. 2012..The pathway, consisting of GPCR, Gβγ, Elmo/Dock, Rac, and Arp2/3, spatially guides the growth of dendritic actin networks in pseudopods of eukaryotic cells during chemotaxis... - Coupling mechanism of a GPCR and a heterotrimeric G protein during chemoattractant gradient sensing in DictyosteliumXuehua Xu
Chemotaxis Signal Section, National Institutes of Health, Rockville, MD 20852, USA
Sci Signal 3:ra71. 2010..We suggest that chemoattractant receptors use a ligand-induced coupling rather than a precoupled mechanism to control the activation of G proteins during chemotaxis... - An Elmo-like protein associated with myosin II restricts spurious F-actin events to coordinate phagocytosis and chemotaxisNilgun Isik
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Twinbrook II Facility, 12331 Parklawn Drive, Rockville, MD 20852, USA
Dev Cell 15:590-602. 2008..Taken together, our data suggest that ElmoA modulates actin/myosin II at the cortex to prevent excessive F-actin polymerization around the cell periphery, thereby maintaining proper cell shape during phagocytosis and chemotaxis... - Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cellsXuehua Xu
Chemotaxis Signal Section, National Institutes of Health, NIAID/LIG/CSS, Rockville, MD, USA
Methods Mol Biol 346:281-96. 2006..Finally, we describe a fluorescence resonance energy transfer (FRET) method to determine the extent of heterotrimeric G protein activation in single living cells in response to various chemoattractant fields... - Key role of local regulation in chemosensing revealed by a new molecular interaction-based modeling methodMartin Meier-Schellersheim
Lymphocyte Biology Section and Program in Systems Immunology and Infectious Disease Modeling, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
PLoS Comput Biol 2:e82. 2006..These features of Simmune were critical to the model development and analysis presented here and are likely to be useful in the computational investigation of many aspects of cell biology... - A vesicle surface tyrosine kinase regulates phagosome maturationJun Fang
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Rockville, MD 20852, USA
J Cell Biol 178:411-23. 2007..Our findings suggest that localized tyrosine kinase signaling on the surface of endosome/lysosomes represents a control mechanism for phagosome maturation... - Ligand-induced partitioning of human CXCR1 chemokine receptors with lipid raft microenvironments facilitates G-protein-dependent signalingXuanmao Jiao
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, 12441 Parklawn Drive, Twinbrook II, Rockville, Maryland 20852, USA
Mol Cell Biol 25:5752-62. 2005..Our results suggest a novel mechanism by which ligand binding to CXCR1 promotes lipid raft partitioning of receptors and facilitates activation of heterotrimeric G-proteins... - HIV gp120-induced interaction between CD4 and CCR5 requires cholesterol-rich microenvironments revealed by live cell fluorescence resonance energy transfer imagingLing Yi
Laboratory of Immunogenetics, Twinbrook II Facility, NIAID, National Institutes of Health, Rockville, Maryland 20852, USA
J Biol Chem 281:35446-53. 2006..Our studies suggest that, during the first step of the HIV entry process, gp120 binding alters the microenvironments of unbound CD4 and CCR5, with plasma membrane cholesterol required for the formation of the HIV entry complex... - Fluorescence resonance energy transfer in living cells reveals dynamic membrane changes in the initiation of B cell signalingHae Won Sohn
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Proc Natl Acad Sci U S A 103:8143-8. 2006..Thus, FRET measurements in living B cells revealed highly dynamic and regulated antigen-induced changes in the plasma membrane, allowing association of the BCR with the earliest components of its signaling cascade... - Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cellsJoseph A Brzostowski
NIH NIAID LIG Imaging Facility, Rockville, Maryland, USA
Curr Protoc Protein Sci . 2009..References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided... - Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in DictyosteliumXuehua Xu
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Mol Biol Cell 16:676-88. 2005..We propose a revised model of gradient sensing, suggesting an important role for locally controlled components that inhibit PI3Kinase activity... - Slamming the DOR on chemokine receptor signaling: heterodimerization silences ligand-occupied CXCR4 and delta-opioid receptorsDale Hereld
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
Eur J Immunol 38:334-7. 2008.... - A shortcut from GPCR signaling to Rac-mediated actin cytoskeleton through an ELMO/DOCK complexXuehua Xu
Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
Small Gtpases 3:183-5. 2012..Recently, we discovered a novel function of an ELMO protein in Dictyostelium discoideum linking GPCR signaling from Gβ to actin dynamics through regulating Rac activation during chemotaxis... - Spontaneous clustering and tyrosine phosphorylation of NK cell inhibitory receptor induced by ligand bindingMathias Faure
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20852, USA
J Immunol 170:6107-14. 2003..The unique property of KIR to become phosphorylated in the absence of adhesion and of actin cytoskeleton rearrangement explains how KIR can efficiently block early activation signals during NK-target cell contacts... - Attenuation of phospholipid signaling provides a novel mechanism for the action of valproic acidXuehua Xu
School of Biological Sciences, Royal Holloway University of London, Egham, United Kingdom
Eukaryot Cell 6:899-906. 2007.... - The neuroprotective agent, valproic acid, regulates the mitogen-activated protein kinase pathway through modulation of protein kinase A signalling in Dictyostelium discoideumKatrina Boeckeler
Department of Biology and the Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK
Eur J Cell Biol 85:1047-57. 2006..This work therefore suggests a conserved pathway in eukaryotes that is targeted by neuroprotective and bipolar disorder drugs and allows us to propose a model for this neuroprotective effect... - Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cellsHuang Chang-zheng
J Dermatol Sci 52:130-2. 2008 - Cutting edge: identification of a pre-ligand assembly domain (PLAD) and ligand binding site in the IL-17 receptorJill M Kramer
Department of Oral Biology, University at Buffalo, State University of New York, Buffalo, NY 14214, USA
J Immunol 179:6379-83. 2007..However, the FN1 domain is also required for high affinity interactions with IL-17. Therefore, although the PLAD is located entirely within FN2, effective ligand binding also involves contributions from the linker and FN1... - Essential roles of receptor-interacting protein and TRAF2 in oxidative stress-induced cell deathHan Ming Shen
Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 24:5914-22. 2004..Therefore, our study uncovers a novel signaling pathway regulating oxidative stress-induced cell death... - Genetic analysis of the role of G protein-coupled receptor signaling in electrotaxisMin Zhao
Department of Biomedical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom
J Cell Biol 157:921-7. 2002....