Research Topics
| John P JakupciakSummaryAffiliation: National Institute of Standards and Technology Country: USA Publications
| Collaborators
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Detail Information
Publications
Real-time telomerase assay of less-invasively collected esophageal cell samplesBrenna M McGruder
Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, 20899, Gaithersburg, MD, USA
Cancer Lett 244:91-100. 2006..These findings demonstrate the feasibility of using the RTTRAP assay in EBC samples and suggest that individuals from high-risk populations can be screened for telomerase activity...
Mitochondrial DNA as a cancer biomarkerJohn P Jakupciak
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Dr, MS 8311, Gaithersburg, MD 20899, USA
J Mol Diagn 7:258-67. 2005..Hence, incomplete mitochondrial genome sequencing, designed to scan discrete portions of the genome, misses potentially important sequence variants associated with cancer or other diseases...- Real-time telomerase activity measurements for detection of cancerJohn P Jakupciak
National Institute of Standards and Technology, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
Expert Rev Mol Diagn 5:745-53. 2005..The hope is that the use of telomerase will finally translate into a diagnostic to help realize longer survival and a better quality of life... - Analysis of potential cancer biomarkers in mitochondrial DNAJohn P Jakupciak
National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
Curr Opin Mol Ther 8:500-6. 2006.... - Performance of mitochondrial DNA mutations detecting early stage cancerJohn P Jakupciak
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
BMC Cancer 8:285. 2008..The detection of mtDNA mutations in body fluids using resequencing microarrays, which are more sensitive than other sequencing methods, could provide a strategy to measure mutation loads in remote anatomical sites... - Preparation and characterization of candidate reference materials for telomerase assaysJohn P Jakupciak
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Clin Chem 51:1443-50. 2005..CONCLUSIONS: We have prepared and characterized a candidate reference material that appears to be suitable for use in a wide range of assays of telomerase activity and expression... - Multiple strand displacement amplification of mitochondrial DNA from clinical samplesSamantha Maragh
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
BMC Med Genet 9:7. 2008..We applied the method to a small group of cancer cases and controls and demonstrated that WGA is capable of increasing the yield of starting DNA material with identical genetic sequence... - Analytical validation of telomerase activity for cancer early detection: TRAP/PCR-CE and hTERT mRNA quantification assay for high-throughput screening of tumor cellsJohn P Jakupciak
NIST, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
J Mol Diagn 6:157-65. 2004..Using this combination of telomerase activity and hTERT mRNA measurements, the automated system improved efficiency over traditional TRAP/PCR methods... - Standards for validation of cancer biomarkersDonald H Atha And
Tetracore, Inc, Gaithersburg, MD 20878, USA
Cancer Biomark 1:233-9. 2005.... - Renewable standard reference material for the detection of TP53 mutationsCatherine D O'Connell
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Mol Diagn 7:85-97. 2003..Their use will improve disease detection by serving as validation materials to monitor errors in measurement methods, including PCR amplification, amplicon separation, and data analysis from different technology platforms... - Advances in Huntington's disease diagnostics: development of a standard reference materialBarbara C Levin
National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Biochemical Science Division, Chemical Science and Technology Laboratory, Gaithersburg, MD 20899 8311, USA
Expert Rev Mol Diagn 6:587-96. 2006..The use of a HD standard reference material will provide the quality control and assurance that data from different laboratories are both comparable and accurate... - The pseudo-mitochondrial genome influences mistakes in heteroplasmy interpretationRyan L Parr
Genesis Genomics Inc, 1294 Balmoral Street, Thunder Bay, Ontario, P7B 5Z5, Canada
BMC Genomics 7:185. 2006..Nuclear mitochondrial pseudogenes (numts) are a potential source of contamination during mitochondrial DNA PCR amplification. This possibility warrants careful experimental design and cautious interpretation of heteroplasmic results... - Development of genomic reference materials for Huntington disease genetic testingLisa Kalman
Laboratory Practice Evaluation and Genomics Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Genet Med 9:719-23. 2007.... - Mitochondrial genome deletion aids in the identification of false- and true-negative prostate needle core biopsy specimensJennifer Maki
Genesis Genomics, Thunder Bay, Canada
Am J Clin Pathol 129:57-66. 2008..In a blinded external validation study, the sensitivity and specificity were 83% and 79%, and the area under the ROC curve was 0.87. The 3.4 mtdelta may be useful in defining malignant, benign, and PTM prostate tissues... - Consensus characterization of 16 FMR1 reference materials: a consortium studyJean Amos Wilson
Sequenom, San Diego, California, USA
J Mol Diagn 10:2-12. 2008..DNA purified from these cell lines is available to the genetics community through the Coriell Cell Repositories. The public availability of these reference materials should help support accurate clinical fragile X syndrome testing... - Facile whole mitochondrial genome resequencing from nipple aspirate fluid using MitoChip v2.0John P Jakupciak
Cipher Systems, Crofton, USA
BMC Cancer 8:95. 2008..0 (MCv2), and 4) assessing the somatic mtDNA mutation rate in benign breast diseases as a potential tool for monitoring early somatic mutations associated with breast cancer... - An optimized microchip electrophoresis system for mutation detection by tandem SSCP and heteroduplex analysis for p53 gene exons 5-9Christa N Hestekin
Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
Electrophoresis 27:3823-35. 2006..Finally, the use of electrical fields between 350 and 450 V/cm provided rapid separations (<10 min) with well-resolved DNA peaks for both SSCP and HA... - Long homopurine*homopyrimidine sequences are characteristic of genes expressed in brain and the pseudoautosomal regionAlbino Bacolla
Institute of Biosciences and Technology, Center for Genome Research, Texas A and M University System Health Science Center, Texas Medical Center, 2121 West Holcombe Blvd, Houston, TX 77030, USA
Nucleic Acids Res 34:2663-75. 2006..These results support a role for long R*Y tracts in promoting recombination and genome diversity during evolution through destabilization of chromosomal DNA, thereby inducing repair and mutation...