John P Jakupciak

Summary

Affiliation: National Institute of Standards and Technology
Country: USA

Publications

  1. ncbi request reprint Real-time telomerase assay of less-invasively collected esophageal cell samples
    Brenna M McGruder
    Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, 20899, Gaithersburg, MD, USA
    Cancer Lett 244:91-100. 2006
  2. pmc Mitochondrial DNA as a cancer biomarker
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Dr, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 7:258-67. 2005
  3. ncbi request reprint Real-time telomerase activity measurements for detection of cancer
    John P Jakupciak
    National Institute of Standards and Technology, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    Expert Rev Mol Diagn 5:745-53. 2005
  4. ncbi request reprint Analysis of potential cancer biomarkers in mitochondrial DNA
    John P Jakupciak
    National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
    Curr Opin Mol Ther 8:500-6. 2006
  5. pmc Performance of mitochondrial DNA mutations detecting early stage cancer
    John P Jakupciak
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    BMC Cancer 8:285. 2008
  6. ncbi request reprint Preparation and characterization of candidate reference materials for telomerase assays
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Clin Chem 51:1443-50. 2005
  7. pmc Multiple strand displacement amplification of mitochondrial DNA from clinical samples
    Samantha Maragh
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    BMC Med Genet 9:7. 2008
  8. ncbi request reprint Standards for validation of cancer biomarkers
    Catherine D O'Connell
    Tetracore, Inc, Gaithersburg, MD 20878, USA
    Cancer Biomark 1:233-9. 2005
  9. pmc Analytical validation of telomerase activity for cancer early detection: TRAP/PCR-CE and hTERT mRNA quantification assay for high-throughput screening of tumor cells
    John P Jakupciak
    NIST, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 6:157-65. 2004
  10. ncbi request reprint Renewable standard reference material for the detection of TP53 mutations
    Catherine D O'Connell
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Mol Diagn 7:85-97. 2003

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Real-time telomerase assay of less-invasively collected esophageal cell samples
    Brenna M McGruder
    Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, 20899, Gaithersburg, MD, USA
    Cancer Lett 244:91-100. 2006
    ..These findings demonstrate the feasibility of using the RTTRAP assay in EBC samples and suggest that individuals from high-risk populations can be screened for telomerase activity...
  2. pmc Mitochondrial DNA as a cancer biomarker
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Dr, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 7:258-67. 2005
    ..Hence, incomplete mitochondrial genome sequencing, designed to scan discrete portions of the genome, misses potentially important sequence variants associated with cancer or other diseases...
  3. ncbi request reprint Real-time telomerase activity measurements for detection of cancer
    John P Jakupciak
    National Institute of Standards and Technology, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    Expert Rev Mol Diagn 5:745-53. 2005
    ..The hope is that the use of telomerase will finally translate into a diagnostic to help realize longer survival and a better quality of life...
  4. ncbi request reprint Analysis of potential cancer biomarkers in mitochondrial DNA
    John P Jakupciak
    National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
    Curr Opin Mol Ther 8:500-6. 2006
    ....
  5. pmc Performance of mitochondrial DNA mutations detecting early stage cancer
    John P Jakupciak
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    BMC Cancer 8:285. 2008
    ..The detection of mtDNA mutations in body fluids using resequencing microarrays, which are more sensitive than other sequencing methods, could provide a strategy to measure mutation loads in remote anatomical sites...
  6. ncbi request reprint Preparation and characterization of candidate reference materials for telomerase assays
    John P Jakupciak
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Clin Chem 51:1443-50. 2005
    ....
  7. pmc Multiple strand displacement amplification of mitochondrial DNA from clinical samples
    Samantha Maragh
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    BMC Med Genet 9:7. 2008
    ..We applied the method to a small group of cancer cases and controls and demonstrated that WGA is capable of increasing the yield of starting DNA material with identical genetic sequence...
  8. ncbi request reprint Standards for validation of cancer biomarkers
    Catherine D O'Connell
    Tetracore, Inc, Gaithersburg, MD 20878, USA
    Cancer Biomark 1:233-9. 2005
    ....
  9. pmc Analytical validation of telomerase activity for cancer early detection: TRAP/PCR-CE and hTERT mRNA quantification assay for high-throughput screening of tumor cells
    John P Jakupciak
    NIST, Biotechnology Division, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899, USA
    J Mol Diagn 6:157-65. 2004
    ..Using this combination of telomerase activity and hTERT mRNA measurements, the automated system improved efficiency over traditional TRAP/PCR methods...
  10. ncbi request reprint Renewable standard reference material for the detection of TP53 mutations
    Catherine D O'Connell
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Mol Diagn 7:85-97. 2003
    ..Their use will improve disease detection by serving as validation materials to monitor errors in measurement methods, including PCR amplification, amplicon separation, and data analysis from different technology platforms...
  11. ncbi request reprint Advances in Huntington's disease diagnostics: development of a standard reference material
    Barbara C Levin
    National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Biochemical Science Division, Chemical Science and Technology Laboratory, Gaithersburg, MD 20899 8311, USA
    Expert Rev Mol Diagn 6:587-96. 2006
    ..The use of a HD standard reference material will provide the quality control and assurance that data from different laboratories are both comparable and accurate...
  12. pmc The pseudo-mitochondrial genome influences mistakes in heteroplasmy interpretation
    Ryan L Parr
    Genesis Genomics Inc, 1294 Balmoral Street, Thunder Bay, Ontario, P7B 5Z5, Canada
    BMC Genomics 7:185. 2006
    ..Nuclear mitochondrial pseudogenes (numts) are a potential source of contamination during mitochondrial DNA PCR amplification. This possibility warrants careful experimental design and cautious interpretation of heteroplasmic results...
  13. ncbi request reprint Development of genomic reference materials for Huntington disease genetic testing
    Lisa Kalman
    Laboratory Practice Evaluation and Genomics Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Genet Med 9:719-23. 2007
    ....
  14. ncbi request reprint Mitochondrial genome deletion aids in the identification of false- and true-negative prostate needle core biopsy specimens
    Jennifer Maki
    Genesis Genomics, Thunder Bay, Canada
    Am J Clin Pathol 129:57-66. 2008
    ..In a blinded external validation study, the sensitivity and specificity were 83% and 79%, and the area under the ROC curve was 0.87. The 3.4 mtdelta may be useful in defining malignant, benign, and PTM prostate tissues...
  15. pmc Consensus characterization of 16 FMR1 reference materials: a consortium study
    Jean Amos Wilson
    Sequenom, San Diego, California, USA
    J Mol Diagn 10:2-12. 2008
    ..DNA purified from these cell lines is available to the genetics community through the Coriell Cell Repositories. The public availability of these reference materials should help support accurate clinical fragile X syndrome testing...
  16. pmc Facile whole mitochondrial genome resequencing from nipple aspirate fluid using MitoChip v2.0
    John P Jakupciak
    Cipher Systems, Crofton, USA
    BMC Cancer 8:95. 2008
    ..0 (MCv2), and 4) assessing the somatic mtDNA mutation rate in benign breast diseases as a potential tool for monitoring early somatic mutations associated with breast cancer...
  17. ncbi request reprint An optimized microchip electrophoresis system for mutation detection by tandem SSCP and heteroduplex analysis for p53 gene exons 5-9
    Christa N Hestekin
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Electrophoresis 27:3823-35. 2006
    ..Finally, the use of electrical fields between 350 and 450 V/cm provided rapid separations (<10 min) with well-resolved DNA peaks for both SSCP and HA...
  18. pmc Long homopurine*homopyrimidine sequences are characteristic of genes expressed in brain and the pseudoautosomal region
    Albino Bacolla
    Institute of Biosciences and Technology, Center for Genome Research, Texas A and M University System Health Science Center, Texas Medical Center, 2121 West Holcombe Blvd, Houston, TX 77030, USA
    Nucleic Acids Res 34:2663-75. 2006
    ..These results support a role for long R*Y tracts in promoting recombination and genome diversity during evolution through destabilization of chromosomal DNA, thereby inducing repair and mutation...