H J Issaq

Summary

Affiliation: National Cancer Institute
Country: USA

Publications

  1. ncbi request reprint An investigation into the human serum "interactome"
    Ming Zhou
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD, USA
    Electrophoresis 25:1289-98. 2004
  2. ncbi request reprint Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, Fredrick, MD, USA
    Electrophoresis 25:1342-52. 2004
  3. ncbi request reprint Global analysis of the cortical neuron proteome
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 3:896-907. 2004
  4. ncbi request reprint Investigation of the mouse serum proteome
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702, USA
    J Proteome Res 4:1561-8. 2005
  5. ncbi request reprint Proteomic analysis of plasma membrane from hypoxia-adapted malignant melanoma
    Luke H Stockwin
    Drug Mechanisms Group and Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    J Proteome Res 5:2996-3007. 2006
  6. ncbi request reprint Methods for fractionation, separation and profiling of proteins and peptides
    Haleem J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 23:3048-61. 2002
  7. ncbi request reprint The role of separation science in proteomics research
    H J Issaq
    Separation Technology Group, SAIC Frederick NCI Frederick, MD 21702, USA
    Electrophoresis 22:3629-38. 2001
  8. ncbi request reprint The SELDI-TOF MS approach to proteomics: protein profiling and biomarker identification
    Haleem J Issaq
    SAIC Frederick, Inc, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA
    Biochem Biophys Res Commun 292:587-92. 2002
  9. doi request reprint Analytical and statistical approaches to metabolomics research
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    J Sep Sci 32:2183-99. 2009
  10. ncbi request reprint SELDI-TOF MS for diagnostic proteomics
    Haleem J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute Frederick, MD 21702, USA
    Anal Chem 75:148A-155A. 2003

Collaborators

Detail Information

Publications76

  1. ncbi request reprint An investigation into the human serum "interactome"
    Ming Zhou
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD, USA
    Electrophoresis 25:1289-98. 2004
    ..Among the 210 proteins identified, 73% and 67% were not found in previous studies of the low-molecular-weight or whole-serum proteome, respectively...
  2. ncbi request reprint Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, Fredrick, MD, USA
    Electrophoresis 25:1342-52. 2004
    ..41-fold increase as determined by Western blotting. These results demonstrate that quantitative proteomics is capable of providing a quantitative view of thousands of proteins in mammalian cells within a defined set of experiments...
  3. ncbi request reprint Global analysis of the cortical neuron proteome
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 3:896-907. 2004
    ..The results presented here provide the broadest proteome coverage for a mammalian cell to date and show that MS-based proteomics has the potential to provide high coverage of the proteins expressed within a cell...
  4. ncbi request reprint Investigation of the mouse serum proteome
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702, USA
    J Proteome Res 4:1561-8. 2005
    ..The results presented here represent the broadest proteome coverage in mouse serum and provide a foundation from which quantitative comparisons can be made in this important animal model...
  5. ncbi request reprint Proteomic analysis of plasma membrane from hypoxia-adapted malignant melanoma
    Luke H Stockwin
    Drug Mechanisms Group and Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    J Proteome Res 5:2996-3007. 2006
    ..Antibody-based analysis of a panel of human melanoma cell lines confirmed that CD13 and SDF-1 were consistently upregulated during hypoxia. This study provides the basis for the discovery of novel hypoxia-induced membrane proteins...
  6. ncbi request reprint Methods for fractionation, separation and profiling of proteins and peptides
    Haleem J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 23:3048-61. 2002
    ....
  7. ncbi request reprint The role of separation science in proteomics research
    H J Issaq
    Separation Technology Group, SAIC Frederick NCI Frederick, MD 21702, USA
    Electrophoresis 22:3629-38. 2001
    ..Also, a discussion of novel approaches to protein concentration, separation, detection, and quantification is given...
  8. ncbi request reprint The SELDI-TOF MS approach to proteomics: protein profiling and biomarker identification
    Haleem J Issaq
    SAIC Frederick, Inc, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA
    Biochem Biophys Res Commun 292:587-92. 2002
    ..In this minireview we discuss the application of SELDI-TOF MS to protein biomarker discovery and profiling...
  9. doi request reprint Analytical and statistical approaches to metabolomics research
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    J Sep Sci 32:2183-99. 2009
    ....
  10. ncbi request reprint SELDI-TOF MS for diagnostic proteomics
    Haleem J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute Frederick, MD 21702, USA
    Anal Chem 75:148A-155A. 2003
  11. doi request reprint Electrophoresis and liquid chromatography/tandem mass spectrometry in disease biomarker discovery
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:1222-8. 2009
    ..A brief discussion of electrophoretic procedures used for protein fractionation and biomarker discovery is also included...
  12. doi request reprint Detection of bladder cancer in human urine by metabolomic profiling using high performance liquid chromatography/mass spectrometry
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    J Urol 179:2422-6. 2008
    ..We evaluated high performance liquid chromatography coupled online with a mass spectrometer metabolomic approach to differentiate urine samples from healthy individuals and patients with bladder cancer...
  13. doi request reprint Utility of separation science in metabolomic studies
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    J Sep Sci 31:1936-47. 2008
    ..The application of each technique is discussed and its advantages and limitations are discussed with selective illustrative examples...
  14. ncbi request reprint The role of electrophoresis in disease biomarker discovery
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 28:1980-8. 2007
    ....
  15. ncbi request reprint Multidimensional separation of peptides for effective proteomic analysis
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland, MA 21702, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 817:35-47. 2005
    ..This manuscript provides a review of two- and three-dimensional peptide separation strategies combined with MS for the analysis of complex peptide mixtures...
  16. ncbi request reprint Multidimensional high performance liquid chromatography--capillary electrophoresis separation of a protein digest: an update
    H J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute at Frederick, MD 21702, USA
    Electrophoresis 22:1133-5. 2001
    ..The total experimental time for the HPLC and capillary electrophoretic analyses ist less than 1 h, which ist much faster than using 2-D slab-gel electrophoresis or single-capillary capillary electrophoresis...
  17. ncbi request reprint Sheathless electrospray ionization interfaces for capillary electrophoresis-mass spectrometric detection advantages and limitations
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 20702, USA
    J Chromatogr A 1053:37-42. 2004
    ..The advantages and limitations of three sheathless designs are discussed and commented upon, these include single-capillary, two-capillary and three-piece designs...
  18. ncbi request reprint A decade of capillary electrophoresis
    H J Issaq
    Separation Technology Group, Chemical Synthesis and Analysis Laboratory, SAIC Frederick, NCI FCRDC, MD 21702, USA
    Electrophoresis 21:1921-39. 2000
    ....
  19. ncbi request reprint The effect of column length, applied voltage, gel type, and concentration on the capillary electrophoresis separation of DNA fragments and polymerase chain reaction products
    H J Issaq
    SAIC Frederik, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 18:1153-8. 1997
    ..The Sigma DNA replaceable gel gave reproducible results when used as received or diluted at 60%. In our hands hydroxyethyl cellulose gave more reproducible results than polyacrylamide gel...
  20. ncbi request reprint Effect of temperature on the separation of DNA fragments by high-performance liquid chromatography and capillary electrophoresis: a comparative study
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr B Biomed Sci Appl 738:243-8. 2000
    ..Also, the optimum HPLC temperature might be different depending on the fragment sizes to be resolved...
  21. ncbi request reprint Capillary electrophoresis of natural products
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 18:2438-52. 1997
    ..A discussion of sample extraction and clean-up and the advantages of using CE is also presented...
  22. ncbi request reprint Capillary electrophoresis of natural products-II
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 20:3190-202. 1999
    ..A section dealing with recent reviews related to natural products is also included...
  23. ncbi request reprint A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 20:1533-7. 1999
    ..This set-up is practical, simple, robust and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of protein digest...
  24. ncbi request reprint Solid-state UV laser-induced fluorescence detection in capillary electrophoresis
    K C Chan
    Analytical Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 21:2062-6. 2000
    ..Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated...
  25. pmc Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS
    Xiaoying Ye
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI at Frederick, Frederick, Maryland 21702 1201, USA
    J Proteomics 73:112-22. 2009
    ..These results indicate that methanol alone or in combination with PPS yielded significantly higher membrane protein identification/enrichment than the PPS alone...
  26. ncbi request reprint Peptide mobility and peptide mapping in capillary zone electrophoresis. Experimental determination and theoretical simulation
    G M Janini
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr A 848:417-33. 1999
    ..The resulting peptide maps were compared with corresponding theoretical simulation...
  27. pmc Optimized method for computing (18)O/(16)O ratios of differentially stable-isotope labeled peptides in the context of postdigestion (18)O exchange/labeling
    Xiaoying Ye
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Maryland 21702, USA
    Anal Chem 82:5878-86. 2010
    ....
  28. ncbi request reprint Electrokinetic chromatography in suppressed electroosmotic flow environment: use of a charged cyclodextrin for the separation of enantiomers and geometric isomers
    G M Janini
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD, USA
    Electrophoresis 17:1575-83. 1996
    ..The separation of aflatoxin isomers and chlorophenol congeners is presented. In the separation of chlorophenols the more hydrophobic trichlorophenols eluted first and the least hydrophobic, phenol, eluted last...
  29. ncbi request reprint High-speed screening of polymerase chain reaction products by capillary electrophoresis
    K C Chan
    Chemical Synthesis and Analysis Laboratory, SAIC Frederick, National Cancer Institute Frederick Cancer Research and Development Center, Maryland 21702, USA
    Anal Biochem 243:133-9. 1996
    ..This high-speed separation, with minimum postrun data processing, is highly desirable for the high-throughput screening of PCR products using a single-capillary CE system...
  30. ncbi request reprint Electrokinetic chromatography without electroosmotic flow
    G M Janini
    SAIC Frederick, NCI-Frederick Cancer Research and Development Center, MD 21702-1201, USA
    J Chromatogr A 792:125-41. 1997
    ..The principle of separation is the same in electrokinetic chromatography with or without electroosmotic flow; however, there are many significant differences that will be highlighted...
  31. ncbi request reprint Proteomic analysis of detergent-resistant membrane rafts
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute, MD, USA
    Electrophoresis 25:1307-18. 2004
    ..A total of 91 (24%) proteins were classified as integral alpha-helical membrane proteins, of which 51 (56%) were predicted to have multiple transmembrane domains...
  32. ncbi request reprint Peptide mapping by capillary zone electrophoresis: how close is theoretical simulation to experimental determination
    G M Janini
    SAIC Frederick, National Cancer Institute at Frederick, MD 21702, USA
    J Chromatogr A 924:291-306. 2001
    ..The resulting maps were compared and contrasted with their experimental counterparts. The potential of this approach and its limitations are discussed...
  33. pmc Modulation of HIV-like particle assembly in vitro by inositol phosphates
    S Campbell
    HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 98:10875-9. 2001
    ..This requirement is not seen in a deleted Gag protein lacking residues 16-99 within the matrix domain...
  34. ncbi request reprint Quantitative analysis of the low molecular weight serum proteome using 18O stable isotope labeling in a lung tumor xenograft mouse model
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, National Cancer Institute at Frederick, SAIC Frederick, Inc, Frederick, Maryland 21702, USA
    J Am Soc Mass Spectrom 16:1221-30. 2005
    ..The methodology adopted in this work provides the means for future quantitative measurements in comparative animal models of disease and in human disease cohorts...
  35. pmc Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Proteome Res 5:349-60. 2006
    ..This result confirms previous, targeted studies suggesting that DRMMs isolated using Triton X-100 and Brij-96 differ in their protein content...
  36. ncbi request reprint Identification of the SELDI ProteinChip human serum retentate by microcapillary liquid chromatography-tandem mass spectrometry
    Ming Zhou
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 5:2207-16. 2006
    ..This analysis resulted in the identification of 383 unique proteins in the WCX-2 serum retentate. Among the proteins identified, 50 (13%) are documented clinical biomarkers with 36 of these (72%) identified from multiple peptides...
  37. pmc Quantitative proteomic analysis of human breast epithelial cells with differential telomere length
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Biochem Biophys Res Commun 356:942-7. 2007
    ..Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions...
  38. ncbi request reprint Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    Nat Protoc 1:2784-90. 2006
    ..The entire procedure--beginning with isolated membrane fraction and finishing with MS data acquisition--takes 4-5 d...
  39. ncbi request reprint Analysis of fullerene-based nanomaterial in serum matrix by CE
    King C Chan
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 28:1518-24. 2007
    ..The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL...
  40. ncbi request reprint Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 6:4150-62. 2007
    ..The study also indicates that evaluation of confidence levels for phosphopeptide identification via the reversed sequence database searching strategy might underestimate the false positive rate...
  41. doi request reprint Separation, detection and quantitation of peptides by liquid chromatography and capillary electrochromatography
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    J Chromatogr A 1216:1825-37. 2009
    ....
  42. ncbi request reprint Comparison of 1D and 2D NMR spectroscopy for metabolic profiling
    Que N Van
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    J Proteome Res 7:630-9. 2008
    ..While acquisition of the 2D data require more time, the data obtained resulted in a more meaningful and comprehensive metabolic profile, aided in metabolite identifications, and minimized ambiguities in peak assignments...
  43. ncbi request reprint Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:862-70. 2004
    ..These peptides resulted in identification of 2563 proteins of which 876 (34%) are classified as membrane proteins...
  44. ncbi request reprint A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome
    Josip Blonder
    SAIC Frederick Inc, Laboratory of Proteomics and Analytical Technologies Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Proteomics 4:31-45. 2004
    ....
  45. ncbi request reprint Direct ampholyte-free liquid-phase isoelectric peptide focusing: application to the human serum proteome
    Zhen Xiao
    Laboratory of Proteomics and Analytical Technologies, National Cancer Institute at Frederick, SAIC Frederick, Frederick, MD 21702, USA
    Electrophoresis 25:128-33. 2004
    ..The results show that the separation strategy is effective for high-throughput characterization of proteins from complex proteomic mixtures...
  46. ncbi request reprint Two-dimensional liquid chromatography-capillary zone electrophoresis-sheathless electrospray ionization-mass spectrometry: evaluation for peptide analysis and protein identification
    George M Janini
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 1201, USA
    Electrophoresis 25:1973-80. 2004
    ..Unlike first-dimensional ion-exchange LC fractionation, where there is a large degree of overlap, the CZE-MS results show less than 15% overlap between neighboring RPLC fractions...
  47. ncbi request reprint On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification
    George M Janini
    Laboratory of Proteomics and Analytical Technologies and Image Analysis Laboratory, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    Anal Chem 75:5984-93. 2003
    ..Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides...
  48. ncbi request reprint Application of separation technologies to proteomics research
    Haleem J Issaq
    Separation Technology Group, Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Adv Protein Chem 65:249-69. 2003
  49. ncbi request reprint A sheathless nanoflow electrospray interface for on-line capillary electrophoresis mass spectrometry
    George M Janini
    Analytical Chemistry Laboratory, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Anal Chem 75:1615-9. 2003
    ..Injection of 25 fmol of [Glu1]-fibrinopeptide B using the new device produced a CE-ESI-MS electropherogram with a signal-to-noise ratio of over 100 for this peptide...
  50. doi request reprint Phosphoproteomics for the discovery of kinases as cancer biomarkers and drug targets
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Proteomics Clin Appl 1:1042-57. 2007
    ..Strategies for global, targeted, and quantitative phosphoproteomics are discussed, and some recent interesting applications are also evaluated...
  51. ncbi request reprint What to do with "one-hit wonders"?
    Timothy D Veenstra
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Electrophoresis 25:1278-9. 2004
  52. ncbi request reprint Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser-induced fluorescence detection
    K C Chan
    SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr A 718:203-10. 1995
    ..Preliminary results show that the MEKC-LIF with direct sample injection is a feasible method for assessing indole profiles in diluted urine and serum...
  53. ncbi request reprint Characterization of the low molecular weight human serum proteome
    Radhakrishna S Tirumalai
    SAIC Frederick Inc, Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 2:1096-103. 2003
    ..The large number of proteins identified demonstrates the efficacy of this method for the removal of large abundant proteins and the enrichment of the LMW serum proteome...
  54. ncbi request reprint Development of a two-dimensional protein-peptide separation protocol for comprehensive proteome measurements
    George M Janini
    Analytical Chemistry Laboratory, Separations Technology Group, SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 787:43-51. 2003
    ..The results show that the separation strategy is effective for high throughput characterization of proteins from complex proteomic mixtures...
  55. ncbi request reprint Serum and plasma proteomics
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    Chem Rev 107:3601-20. 2007
  56. doi request reprint Clinical and pharmaceutical applications of packed-column supercritical fluid chromatography
    Eric Abbott
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    J Sep Sci 31:1223-30. 2008
    ..This review will discuss recently published applications of pSFC, with a specific focus on its advantages and limitations for the analysis of pharmaceuticals with varying chemical properties...
  57. ncbi request reprint Proteomic patterns: their potential for disease diagnosis
    Zhen Xiao
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702, USA
    Mol Cell Endocrinol 230:95-106. 2005
    ..This review will discuss the basics of SELDI protein chip technology and highlight its recent applications in disease biomarker discovery with emphasis on cancer diagnosis...
  58. ncbi request reprint Evaluation of the acid-cleavable isotope-coded affinity tag reagents: application to camptothecin-treated cortical neurons
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:469-77. 2004
    ..The new ICAT reagents were then applied to the analysis of a cortical neuron proteome sample to identify proteins regulated by the antitumor drug, camptothecin...
  59. ncbi request reprint The use of urine proteomic and metabonomic patterns for the diagnosis of interstitial cystitis and bacterial cystitis
    Que N Van
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Dis Markers 19:169-83. 2003
    ..Using this bioinformatic approach, we were able to discriminate spectral patterns associated with IC-affected, BC-affected, and unaffected patients with a success rate of approximately 84%...
  60. ncbi request reprint Current strategies for quantitative proteomics
    Thomas P Conrads
    Biomedical Proteomics Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Adv Protein Chem 65:133-59. 2003
  61. ncbi request reprint Proteomic patterns as a diagnostic tool for early-stage cancer: a review of its progress to a clinically relevant tool
    Thomas P Conrads
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, MD 21702 1201, USA
    Mol Diagn 8:77-85. 2004
    ..In addition, a critical assessment of the overall technology is presented with an emphasis on the steps required to enable proteomic pattern analysis to become a viable clinical tool for diagnosing early-stage cancers...
  62. ncbi request reprint Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cells
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 4:523-31. 2005
    ..The quantitative profiling of Ib-induced DRMP from Vero cells revealed several proteins with altered expression levels suggesting their possible role in Ib binding/uptake...
  63. ncbi request reprint A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland, USA
    J Invest Dermatol 123:691-9. 2004
    ..Furthermore, 121 proteins from cholesterol-rich plasma membrane domains (caveolar and lipid rafts) were identified...
  64. ncbi request reprint New tools for quantitative phosphoproteome analysis
    Thomas P Conrads
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute at Frederick, Maryland 21702, USA
    Biochem Biophys Res Commun 290:885-90. 2002
    ..This review discusses some of the recent progress made in the development of techniques not only to identify, but also to quantitatively determine sites of phosphorylation...
  65. ncbi request reprint Proteomic investigation of natural killer cell microsomes using gas-phase fractionation by mass spectrometry
    Josip Blonder
    Biomedical Proteomics Program, Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702, USA
    Biochim Biophys Acta 1698:87-95. 2004
    ..e., m/z 400-2000). The results show that GPF(m/z) represents an effective technique for increasing protein identifications in global proteomic investigations especially when sample quantity is limited...
  66. ncbi request reprint A comparison of microLC/electrospray ionization-MS and GC/MS for the measurement of stable isotope enrichment from a [2H2]-glucose metabolic probe in T-cell genomic DNA
    S D Fox
    Analytical Chemistry Laboratory, SAIC Frederick, Inc, National Cancer Institute at Frederick, National Institutes of Health, P O Box B, Frederick, Maryland 21702 1201, USA
    Anal Chem 75:6517-22. 2003
    ..2% [(2)H(2)]-dA enrichment to 5 pmol at 0.1% [(2)H(2)]-dA enrichment)...
  67. ncbi request reprint Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts
    Zhen Xiao
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    J Cell Physiol 210:325-35. 2007
    ..This investigation represents the first systematic proteomic analysis of MVs and provides insights into both the function and origin of these important mineralization-regulating vesicles...
  68. ncbi request reprint Measuring fifteen endogenous estrogens simultaneously in human urine by high-performance liquid chromatography-mass spectrometry
    Xia Xu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Anal Chem 77:6646-54. 2005
    ....
  69. doi request reprint Multiplexed quantitation of endogenous estrogens and estrogen metabolites in human peritoneal fluid
    Xia Xu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Electrophoresis 29:2706-13. 2008
    ..Although micellar EKC gave baseline separation of a standard mixture of 10 EM, the LOQs using UV detection were not suitable for the assay of the low level estrogens in biological samples...
  70. pmc CD4 T cell survival after intermittent interleukin-2 therapy is predictive of an increase in the CD4 T cell count of HIV-infected patients
    Sarah W Read
    Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA
    J Infect Dis 198:843-50. 2008
    ..Thus, an increase in the survival of CD4 T cells appears to be the critical mechanism leading to sustained increases in the CD4 cell counts of HIV-infected patients receiving intermittent IL-2 therapy...
  71. pmc Lsh, an epigenetic guardian of repetitive elements
    Jiaqiang Huang
    Laboratory of Molecular Immunoregulation, SAIC Basic Research Program, National Cancer Institute, Frederick, MD 21701, USA
    Nucleic Acids Res 32:5019-28. 2004
    ..These data suggest that the repetitive sites are direct targets of Lsh action and that Lsh plays an important role as 'epigenetic guardian' of the genome to protect against deregulation of parasitic retroviral elements...
  72. ncbi request reprint Quantitative determination of antalarmin, a novel corticotropin-releasing hormone receptor-1 antagonist, in canine plasma by HPLC-MS
    Stephen D Fox
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702 1201, USA
    J Sep Sci 28:332-6. 2005
    ..Accuracy and precision variation for intra and inter-day validation were below the acceptable limit (15%) for pharmacokinetic studies...
  73. ncbi request reprint Quantitative measurement of endogenous estrogens and estrogen metabolites in human serum by liquid chromatography-tandem mass spectrometry
    Xia Xu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Anal Chem 79:7813-21. 2007
    ....
  74. ncbi request reprint Two dimensional mapping of cancer cell extracts by liquid chromatography-capillary electrophoresis with ultraviolet absorbance detection
    Yan He
    Ames Laboratory USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    J Chromatogr A 979:81-9. 2002
    ..In this way, high-resolution analysis of even the minor components in the complicated mixture was possible...
  75. ncbi request reprint Analysis of fifteen estrogen metabolites using packed column supercritical fluid chromatography-mass spectrometry
    Xia Xu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Anal Chem 78:1553-8. 2006
    ..5 (S/N = 3), and 5 pg, respectively. Compared with RP-HPLC MS analysis of the same mixture in terms of speed of analysis and sensitivity, pSFC MS is much faster, 10 versus 70 min, with comparable LOD and LOQ...
  76. pmc Parallel analysis of transcript and translation profiles: identification of metastasis-related signal pathways differentially regulated by drug and genetic modifications
    Haiyan Yang
    Laboratory of Population Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 5:1555-67. 2006
    ....