Ying Huang

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe
    Y Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development NIH, Building 6, Room 3403, 6 Center Drive MSC 2753, Bethesda, MD 20892 2753, USA
    Yeast 18:463-8. 2001
  2. pmc Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe
    Ying Huang
    Nucleic Acids Res 30:1465-82. 2002
  3. pmc Hepatic gene expression during treatment with peginterferon and ribavirin: Identifying molecular pathways for treatment response
    Jordan J Feld
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Hepatology 46:1548-63. 2007
  4. pmc Barrier-to-autointegration factor (BAF) condenses DNA by looping
    Dunja Skoko
    Laboratory of Molecular Biology, Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:16610-5. 2009
  5. ncbi request reprint Structural basis for dimerization of LAP2alpha, a component of the nuclear lamina
    Christina Marchetti Bradley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 15:643-53. 2007
  6. ncbi request reprint Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, US National Institutes of Health, 31 Center Dr, Rm 2A25, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:611-8. 2006
  7. ncbi request reprint RNA polymerase III from the fission yeast, Schizosaccharomyces pombe
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Methods Enzymol 370:165-73. 2003
  8. pmc Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, NIH, 31 Center Dr, Room 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 25:621-36. 2005
  9. ncbi request reprint Solution NMR structure of the barrier-to-autointegration factor-Emerin complex
    Mengli Cai
    Laboratories of Chemical Physics and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 0520, USA
    J Biol Chem 282:14525-35. 2007
  10. pmc Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 use different receptor complexes to enter T cells
    Kathryn S Jones
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Maryland 21702, USA
    J Virol 80:8291-302. 2006

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe
    Y Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development NIH, Building 6, Room 3403, 6 Center Drive MSC 2753, Bethesda, MD 20892 2753, USA
    Yeast 18:463-8. 2001
    ..The function and applicability of these vectors were examined and the results are shown using the N-terminal tagging vector encoding Sfc6p, a subunit of the Sz. pombe RNA polymerase III general transcription factor, TFIIIC...
  2. pmc Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe
    Ying Huang
    Nucleic Acids Res 30:1465-82. 2002
    ..cerevisiae. Silencing proteins from both yeasts are also actively involved in other chromosome-related nuclear functions, including DNA repair and the regulation of chromatin structure...
  3. pmc Hepatic gene expression during treatment with peginterferon and ribavirin: Identifying molecular pathways for treatment response
    Jordan J Feld
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Hepatology 46:1548-63. 2007
    ..Collectively, these mechanisms may provide a molecular basis for the improved efficacy of combination therapy...
  4. pmc Barrier-to-autointegration factor (BAF) condenses DNA by looping
    Dunja Skoko
    Laboratory of Molecular Biology, Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:16610-5. 2009
    ..Thus the DNA binding properties of BAF may alone be sufficient to account for its association with the preintegration complex...
  5. ncbi request reprint Structural basis for dimerization of LAP2alpha, a component of the nuclear lamina
    Christina Marchetti Bradley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 15:643-53. 2007
    ..The surface involved in binding lamin A/C is proposed based on results from alanine-scanning mutagenesis and a solid-phase overlay binding assay...
  6. ncbi request reprint Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, US National Institutes of Health, 31 Center Dr, Rm 2A25, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:611-8. 2006
    ..The two activities of La in tRNA maturation map to its two conserved RNA-binding surfaces and suggest a modular model that has implications for its other ligands...
  7. ncbi request reprint RNA polymerase III from the fission yeast, Schizosaccharomyces pombe
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Methods Enzymol 370:165-73. 2003
  8. pmc Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, NIH, 31 Center Dr, Room 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 25:621-36. 2005
    ..The data indicate that Rpc11p limits RNA 3'-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast...
  9. ncbi request reprint Solution NMR structure of the barrier-to-autointegration factor-Emerin complex
    Mengli Cai
    Laboratories of Chemical Physics and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 0520, USA
    J Biol Chem 282:14525-35. 2007
    ..The relevance of the implications of the structural and biophysical data on the complex in the context of the interaction between the BAF(2) dimer and Em(LEM) at the nuclear envelope is discussed...
  10. pmc Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 use different receptor complexes to enter T cells
    Kathryn S Jones
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Maryland 21702, USA
    J Virol 80:8291-302. 2006
    ....
  11. ncbi request reprint Phosphorylation of hepatitis C virus NS5A nonstructural protein: a new paradigm for phosphorylation-dependent viral RNA replication?
    Ying Huang
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Virology 364:1-9. 2007
    ..Understanding the process of NS5A phosphorylation and the definite identification of the culprit cellular protein kinase(s) will shed light on the mechanisms of HCV RNA replication and/or pathogenesis...
  12. pmc Regulation of mitochondrial oxidative metabolism by tumor suppressor FLCN
    Hisashi Hasumi
    Urologic Oncology Branch, National Cancer Institute, 10 Center Dr, MSC 1107, CRC Rm 1 5940W, Bethesda, MD 20892, USA
    J Natl Cancer Inst 104:1750-64. 2012
    ..Genetic studies of BHD patients have uncovered the causative gene, FLCN, but its function is incompletely understood...
  13. ncbi request reprint Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptors
    Qi Wang
    Steriod Hormones Section, National Institute of Diabetes and Digestive and Kidney Disease Laboratory of Molecular and Cellular Biology, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Endocrinol 18:1376-95. 2004
    ..This modulation provides an attractive mechanism for differential control of gene expression during development, differentiation, homeostasis, and endocrine therapies...
  14. pmc Defective hepatic response to interferon and activation of suppressor of cytokine signaling 3 in chronic hepatitis C
    Ying Huang
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Gastroenterology 132:733-44. 2007
    ..To understand IFN-alpha resistance in vivo, we examined the dynamic responses to both type I and type II IFNs, human IFN (hIFN)-alpha, -gamma, and consensus IFN, in the chimpanzee model...
  15. pmc The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Nucleic Acids Res 31:2108-16. 2003
    ..Since previous whole genome analysis also revealed TATA elements upstream of tRNA genes in Arabidopsis, this pathway may be more widespread than appreciated previously...
  16. ncbi request reprint Induction of human T cell leukemia virus type I receptors on quiescent naive T lymphocytes by TGF-beta
    Kathryn S Jones
    Basic Research Program, Science Applications International Corporation Frederick, Inc, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Immunol 174:4262-70. 2005
    ....
  17. ncbi request reprint [A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro]
    Ying Huang
    Key Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400016, China
    Zhonghua Gan Zang Bing Za Zhi 13:808-10. 2005
    ..To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase...
  18. ncbi request reprint TC21 mediates transformation and cell survival via activation of phosphatidylinositol 3-kinase/Akt and NF-kappaB signaling pathway
    Rong Rong
    Department of Pharmacology, State University of New York, Upstate Medical University, 750 E Adams Street, Syracuse, NY 13210, USA
    Oncogene 21:1062-70. 2002
    ..In conclusion, we have identified PI3-K as an effector of TC21 and demonstrated that the PI3-K/Akt signaling pathway plays important roles in TC21-mediated transformation and cell survival...
  19. ncbi request reprint Novel poly(ethylene imine) biscarbamate conjugate as an efficient and nontoxic gene delivery system
    Songlin Xu
    School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China
    J Control Release 130:64-8. 2008
    ..PEIC can also transfect MeWo cells with large genes such as the 125kb Varicella-Zoster viral gene (VZV) at high activity...
  20. ncbi request reprint The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell-cycle arrest in T-cells
    Yafeng Zhang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, People s Republic of China
    Biochem J 413:239-50. 2008
    ..Altogether, our results demonstrate that DnaJB6 is necessary for translocation of Slfn1 into the nucleus, where Slfn1 down-regulates cyclin D1, induces cell-cycle arrest and programmes a quiescent state of T-cells...
  21. pmc Similar regulation of cell surface human T-cell leukemia virus type 1 (HTLV-1) surface binding proteins in cells highly and poorly transduced by HTLV-1-pseudotyped virions
    Kathryn S Jones
    Basic Research Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 76:12723-34. 2002
    ..These results suggest that factors other than the number of primary binding receptors are responsible for the differences in the titers of HTLV-1 pseudotypes between highly susceptible cells and poorly susceptible cells...
  22. ncbi request reprint Retinoic acid-induced AP-1 transcriptional activity regulates B16 mouse melanoma growth inhibition and differentiation
    Ying Huang
    Department of Biochemistry and Molecular Biology, Joan C Edwards School of Medicine, Marshall University, Huntington, West Virginia 25704, USA
    J Cell Physiol 194:162-70. 2003
    ..Our observations suggest that AP-1 transcriptional activity induced by RA likely plays an important role in the biological changes mediated by this retinoid in B16 melanoma cells...
  23. ncbi request reprint Structured to reduce the mitogenicity of anti-CD3 antibody based on computer-guided molecular design
    Ming Lv
    Institute of Basic Medical Sciences, Beijing, PR China
    Int J Biochem Cell Biol 39:1142-55. 2007
    ....
  24. ncbi request reprint Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma
    Ying Huang
    Human Cancer Genetics Program, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
    Int J Cancer 104:735-44. 2003
    ..In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. MT1G and CRABP1 are novel genes that are likely involved in the pathogenesis of sporadic PTC...
  25. pmc Receptor isoform and ligand-specific modulation of dihydrotestosterone-induced prostate specific antigen gene expression and prostate tumor cell growth by estrogens
    Yuan Shan Zhu
    Department of Medicine Endocrinology, Weill Medical College of Cornell University, 1300 York Ave, Box 149, New York, NY 10021, USA
    J Androl 26:500-8; discussion 509-10. 2005
    ....
  26. ncbi request reprint An efficient and targeted gene integration system for high-level antibody expression
    Ying Huang
    Department of Molecular Immunology, Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China
    J Immunol Methods 322:28-39. 2007
    ..Three kinds of recombinant antibodies were successfully expressed in candidate cell line 37. The highest producers produced more than 200 mug/ml of the antibody in 6 days of continuous culture in a spinner flask...
  27. pmc Heparan sulfate proteoglycans mediate attachment and entry of human T-cell leukemia virus type 1 virions into CD4+ T cells
    Kathryn S Jones
    Basic Research Program, SAIC Frederick, National Cancer Institute Frederick, MD 21702 1201, USA
    J Virol 79:12692-702. 2005
    ..Thus, HSPGs play a critical role in the binding and entry of HTLV-1 into CD4(+) T cells...
  28. ncbi request reprint Binding activity difference of anti-CD20 scFv-Fc fusion protein derived from variable domain exchange
    Shu Sheng Geng
    College of Life Science, Hebei University, Baoding, Hebei, China
    Cell Mol Immunol 3:439-43. 2006
    ..The study highlights the potential relationship between the antibody binding activity and their 3-D conformation, which appears to be worthwhile in providing direction for future antibody design of recombinant antibody...
  29. ncbi request reprint Cloning and characterization of a novel gene PDRG that is differentially regulated by p53 and ultraviolet radiation
    Xiuquan Luo
    Department of Pharmacology, State University of New York, Upstate Medical University, Syracuse, NY 12310, USA
    Oncogene 22:7247-57. 2003
    ..Furthermore, stable expression of exogenous PDRG was found to decrease the clonogenic survival after UV irradiation, which highlights the significance of PDRG in facilitating UV-induced killing...
  30. ncbi request reprint Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK
    Ke Wen Zhao
    Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis, Chinese Ministry of Education, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine formerly Shanghai Second Medical University, Shanghai 200025, China
    J Biol Chem 280:42707-14. 2005
    ..In conclusion, our results suggest that the induction of PLSCR1 transcription through STAT1 depends upon sequential activation of PKCdelta and JNK...
  31. ncbi request reprint Calcium-sensing receptor ubiquitination and degradation mediated by the E3 ubiquitin ligase dorfin
    Ying Huang
    Department of Biology, Syracuse University, Syracuse, New York 13244, USA
    J Biol Chem 281:11610-7. 2006
    ..Because endogenous CaR in Madin-Darby canine kidney cells is also subject to degradation from the endoplasmic reticulum, dorfin-mediated ubiquitination may contribute to a general mechanism for CaR quality control during biosynthesis...
  32. ncbi request reprint Regulation of recombinant monoclonal antibody production in chinese hamster ovary cells: a comparative study of gene copy number, mRNA level, and protein expression
    Zhou Jiang
    The Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Biotechnol Prog 22:313-8. 2006
    ..We also observed that the HC to LC gene copy ratio may affect the protein yield in MAb expression systems...
  33. ncbi request reprint Cyclooxygenase-2 interacts with p53 and interferes with p53-dependent transcription and apoptosis
    Chad A Corcoran
    Department of Pharmacology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA
    Oncogene 24:1634-40. 2005
    ..Based on our results, we propose that p53-dependent upregulation and activation of COX-2 appear to be yet another novel mechanism by which p53 could abate its own growth-inhibitory and apoptotic effects...
  34. ncbi request reprint Alteration of protein subcellular location and domain formation by alternative translational initiation
    Jun Cai
    Institute of Bioinformatics, MOE Key Laboratory of Bioinformatics, Department of Automation, Tsinghua University, Beijing, China
    Proteins 62:793-9. 2006
    ..The peculiar consequence of subcellular relocation as well as change of domain contents reflected the very high level of biological complexity as alternative usage of initiation codons...