Research Topics
Species | Ruili HuangSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
|
Detail Information
Publications
Linking tumor cell cytotoxicity to mechanism of drug action: an integrated analysis of gene expression, small-molecule screening and structural databasesDavid G Covell
National Cancer Institute Frederick, Developmental Therapeutics Program, Screening Technologies Branch, Laboratory of Computational Technologies, Frederick, Maryland, USA
Proteins 59:403-33. 2005..e., few) molecular targets. Applications of data mining strategies that jointly utilize tumor cell screening, genomic, and structural data are presented for hypotheses generation and identifying novel anticancer candidates...- Comprehensive analysis of pathway or functionally related gene expression in the National Cancer Institute's anticancer screenRuili Huang
Laboratory of Computational Technologies, Developmental Therapeutics Program, Screening Technologies Branch, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA
Genomics 87:315-28. 2006..The knowledge of the nature of gene expression regulation and biological pathways can be applied to understanding the mechanism by which small drug molecules interfere with biological systems... - Evaluating chemical structure similarity as an indicator of cellular growth inhibitionAnders Wallqvist
National Cancer Institute, Developmental Therapeutics Program, Screening Technologies Branch, Frederick, Maryland 21702, USA
J Chem Inf Model 46:430-7. 2006..Structurally and functionally similar compounds can have distinguishable biological responses reflecting different mechanisms of action... - Assessment of in vitro and in vivo activities in the National Cancer Institute's anticancer screen with respect to chemical structure, target specificity, and mechanism of actionRuili Huang
Developmental Therapeutics Program, Screening Technologies Branch, Laboratory of Computational Technologies, National Cancer Institute-Frederick, Frederick, Maryland 21702, USA
J Med Chem 49:1964-79. 2006..Finally, we offer a strategy to exploit this relationship for future mining of novel anticancer candidates... - Anticancer medicines in development: assessment of bioactivity profiles within the National Cancer Institute anticancer screening dataDavid G Covell
National Cancer Institute Frederick, Developmental Therapeutics Program, Screening Technologies Branch, Laboratory of Computational Technologies, Frederick, MD 21702, USA
Mol Cancer Ther 6:2261-70. 2007..These results are extended to applications of two-dimensional structural features to further refine compound selections based on tumor panel sensitivity obtained from tumor screening results... 
Chemical genomics profiling of environmental chemical modulation of human nuclear receptorsRuili Huang
National Institutes of Health Chemical Genomics Center, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 3370, USA
Environ Health Perspect 119:1142-8. 2011....- Weighted feature significance: a simple, interpretable model of compound toxicity based on the statistical enrichment of structural featuresRuili Huang
Department of Health and Human Services, NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
Toxicol Sci 112:385-93. 2009..The new algorithm has the important advantages of simplicity, power, interpretability, and ease of implementation... - Characterization of diversity in toxicity mechanism using in vitro cytotoxicity assays in quantitative high throughput screeningRuili Huang
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
Chem Res Toxicol 21:659-67. 2008..The performance of this clustering method is evaluated by comparing the clustering results against literature annotations of compound mechanisms... - Application of a homogenous membrane potential assay to assess mitochondrial functionSrilatha Sakamuru
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
Physiol Genomics 44:495-503. 2012..Our results demonstrate that this homogenous cell-based Mito-MPS assay can be used to evaluate the ability of large numbers of chemicals to decrease mitochondrial function... - Identification of small molecule compounds that inhibit the HIF-1 signaling pathwayMenghang Xia
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892 3370, USA
Mol Cancer 8:117. 2009..To identify small molecule inhibitors of the HIF-1 pathway, we have developed a cell-based reporter gene assay and screened a large compound library by using a quantitative high-throughput screening (qHTS) approach... - Chemical genomic profiling for antimalarial therapies, response signatures, and molecular targetsJing Yuan
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 333:724-9. 2011..Drugs whose responses mapped to wild-type or mutant pfcrt alleles were tested in combination in vitro and in vivo, which yielded promising new leads for antimalarial treatments... - Identification of quaternary ammonium compounds as potent inhibitors of hERG potassium channelsMenghang Xia
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892 3370, USA
Toxicol Appl Pharmacol 252:250-8. 2011..Profiling environmental compound libraries for hERG channel inhibition provides information useful in prioritizing these compounds for cardiotoxicity assessment in vivo... - Compound cytotoxicity profiling using quantitative high-throughput screeningMenghang Xia
NIH Chemical Genomics Center, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 3370, USA
Environ Health Perspect 116:284-91. 2008..Such methods can be relatively expensive, low-throughput, and associated with pain suffered by the treated animals. In addition, differences in species biology may confound extrapolation to human health effects... - Exploration and optimization of substituted triazolothiadiazines and triazolopyridazines as PDE4 inhibitorsAmanda P Skoumbourdis
NIH Chemical Genomics Center, National Human Genome Research Institute, NIH 9800 Medical Center Drive, MSC 3370 Bethesda, MD 20892 3370, USA
Bioorg Med Chem Lett 19:3686-92. 2009..Finally, docking studies with selective ligands (including 10 and 18) were undertaken to better understand this chemotypes ability to bind and inhibit PDE4 selectively... - Identification of compounds that potentiate CREB signaling as possible enhancers of long-term memoryMenghang Xia
NIH Chemical Genomics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:2412-7. 2009..qHTS followed by interrogation of pathway targets is an efficient paradigm for lead generation for chemical genomics and drug development... - The NCGC pharmaceutical collection: a comprehensive resource of clinically approved drugs enabling repurposing and chemical genomicsRuili Huang
National Institutes of Health Chemical Genomics Center, NIH, Bethesda, MD 20892, USA
Sci Transl Med 3:80ps16. 2011..We report here the creation of a definitive, complete, and nonredundant list of all approved molecular entities as a freely available electronic resource and a physical collection of small molecules amenable to high-throughput screening... - Identification of chemical compounds that induce HIF-1alpha activityMenghang Xia
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland 20892, USA
Toxicol Sci 112:153-63. 2009..Identification of environmental compounds having HIF-1alpha activation activity in cell-based assays may be useful for prioritizing chemicals for further testing as hypoxia-response inducers in vivo... - A bioluminescent cytotoxicity assay for assessment of membrane integrity using a proteolytic biomarkerMing Hsuang Cho
NIH Chemical Genomics Center, National Institutes of Health, 9800 Medical Center Drive, MSC 3370, Bethesda, MD 20892 3370, USA
Toxicol In Vitro 22:1099-106. 2008..This cytotoxicity assay, combined with the qHTS platform, allowed us to quickly and efficiently evaluate compound toxicities related to cell membrane integrity... - Profiling environmental chemicals for activity in the antioxidant response element signaling pathway using a high throughput screening approachSunita J Shukla
NIH Chemical Genomics Center, National Institutes of Health, Department of Health and Human Services, Rockville, Maryland, USA
Environ Health Perspect 120:1150-6. 2012..Thus, assays that detect the up-regulation of this pathway could be useful for identifying chemicals that induce oxidative stress... - Identification of clinically used drugs that activate pregnane X receptorsSunita J Shukla
National Institutes of Health Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
Drug Metab Dispos 39:151-9. 2011.... - Identification of known drugs that act as inhibitors of NF-kappaB signaling and their mechanism of actionSusanne C Miller
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Pharmacol 79:1272-80. 2010..Comprehensive profiling of approved drugs provides insight into their molecular mechanisms, thus providing a basis for drug repurposing... 
Synthesis and evaluation of quinazolin-4-ones as hypoxia-inducible factor-1α inhibitorsWenwei Huang
NIH Chemical Genomics Center, National Human Genome Research Institute, NIH, 9800 Medical Center Dr, Rockville, MD 20850, USA
Bioorg Med Chem Lett 21:5239-43. 2011..In this Letter, we describe an efficient one-pot sequential reaction for the synthesis of quinazolin-4-one 1 analogues. The structure-activity relationship (SAR) study led to the 5-fold more potent analogue, 16...- High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell deathJennifer T Fox
Genome Instability Section, Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 109:5423-8. 2012..Furthermore, resveratrol and genistein killed multidrug-resistant cancer cells. We therefore propose that resveratrol, genistein, and baicalein are attractive candidates for improved chemotherapeutic agents... - The pilot phase of the NIH Chemical Genomics CenterCraig J Thomas
NIH Chemical Genomics Center, NHGRI, National Institutes of Health, 9800 Medical Center Drive, Building B, Room 3005, MSC 3370, Bethesda, MD 20892 3370, USA
Curr Top Med Chem 9:1181-93. 2009.... - Identification of a potent new chemotype for the selective inhibition of PDE4Amanda P Skoumbourdis
NIH Chemical Genomics Center, National Human Genome Research Institute, NIH, 9800 Medical Center Drive, MSC 3370, Bethesda, MD 20892 3370, USA
Bioorg Med Chem Lett 18:1297-303. 2008..Synthesis, structure-activity relationships, and the selectivity of a highly potent analogue against related phosphodiesterase isoforms are presented... - Inhibition of morphine-induced cAMP overshoot: a cell-based assay model in a high-throughput formatMenghang Xia
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Cell Mol Neurobiol 31:901-7. 2011..The qHTS approach we used in this study will be useful in identifying novel inhibitors of morphine induced addiction from a larger scale screening... - Predictive models for cytochrome p450 isozymes based on quantitative high throughput screening dataHongmao Sun
National Institutes of Health, Chemical Genomics Center, NIH, Bethesda, Maryland 20892, United States
J Chem Inf Model 51:2474-81. 2011..These models can be useful in prioritizing compounds in a drug discovery pipeline or recognizing the toxic potential of environmental chemicals... - A quantitative high-throughput screen identifies novel inhibitors of the interaction of thyroid receptor beta with a peptide of steroid receptor coactivator 2Ronald L Johnson
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
J Biomol Screen 16:618-27. 2011..Selected compounds were tested as independent samples, and a methylsulfonylnitrobenzoate series inhibited the TRβ-SRC2 interaction with 5 µM IC(50). This series represents a new class of thyroid hormone receptor-coactivator modulators... - Phosphodiesterase 4 inhibitors enhance sexual pleasure-seeking activity in rodentsPeixiong Yuan
Biomarker Laboratory, National Institute of Mental Health, Mood and Anxiety Disorders Program, National Institutes of Health, Bethesda, MD 20892, USA
Pharmacol Biochem Behav 98:349-55. 2011..The results suggest that PDE4 may be a plausible contributor to the sexual pleasure-seeking deficits seen in depressed patients; inhibiting PDE4 may restore these deficits... - Genetic mapping of targets mediating differential chemical phenotypes in Plasmodium falciparumJing Yuan
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
Nat Chem Biol 5:765-71. 2009..This study identifies new leads for antimalarial drugs and demonstrates the utility of a high-throughput chemical genomic strategy for studying malaria traits... - Comprehensive characterization of cytochrome P450 isozyme selectivity across chemical librariesHenrike Veith
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland, USA
Nat Biotechnol 27:1050-5. 2009..We also identified chemical substructures that differentiated between the five isozymes. The pharmacological compendium described here should further the understanding of CYP isozymes... - A quantitative high-throughput screen for modulators of IL-6 signaling: a model for interrogating biological networks using chemical librariesRonald L Johnson
NIH Chemical Genomics Center, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biosyst 5:1039-50. 2009..Small molecules within these series will make useful tool compounds to investigate IL-6 signaling mediated by JAK-STAT activation... - A new homogeneous high-throughput screening assay for profiling compound activity on the human ether-a-go-go-related gene channelSteven A Titus
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Anal Biochem 394:30-8. 2009..Our findings indicate that this thallium flux assay can be used as an alternative method to profile large-volume compound libraries for compound activity on the hERG channel... - Paradigm shift in toxicity testing and modelingHongmao Sun
Department of Health and Human Services, NIH Chemical Genomics Center, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
AAPS J 14:473-80. 2012..It is perceivable that new in silico toxicity models based on high-quality qHTS data will achieve unprecedented reliability and robustness, thus becoming a valuable tool for risk assessment and drug discovery... - The future of toxicity testing: a focus on in vitro methods using a quantitative high-throughput screening platformSunita J Shukla
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892 3370, USA
Drug Discov Today 15:997-1007. 2010..Here, we describe the Tox21 collaboration, qHTS-based compound testing and the various Tox21 screening assays that have been validated and tested at the NCGC to date... - An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cellsWenge Zhu
National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 2753, USA
Mol Cancer Res 9:294-310. 2011..Thus, this assay provides a new approach to the discovery of compounds useful for investigating the regulation of genome duplication and for the treatment of cancer... - Dose-response modeling of high-throughput screening dataFred Parham
National Institutes of Health NIH National Institute of Environmental Health Sciences NIEHS, Research Triangle Park, North Carolina 27709, USA
J Biomol Screen 14:1216-27. 2009..Various statistical tests for identifying significant concentration-response relationships and for addressing reproducibility are developed and presented... - A class of tricyclic compounds blocking malaria parasite oocyst development and transmissionRichard T Eastman
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Antimicrob Agents Chemother 57:425-35. 2013..Further clinical evaluation of ketotifen and related compounds, including synthetic new derivatives, in blocking malaria transmission may provide new weapons for the current effort of malaria eradication... - Fluorescence spectroscopic profiling of compound librariesAnton Simeonov
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892 3370, USA
J Med Chem 51:2363-71. 2008..Native compound fluorescence, fluorescent impurities, novel fluorescent compounds, and the utilization of fluorescence profiling data are discussed... - A grid algorithm for high throughput fitting of dose-response curve dataYuhong Wang
National Institutes of Health, NIH Chemical Genomics Center, 9800 Medical Center Drive, Rockville, MD 20850, USA
Curr Chem Genomics 4:57-66. 2010.... - Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 CellsKristin P Leister
NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Curr Chem Genomics 5:21-9. 2011..We also identified several novel inhibitors of TNF-α, such as BTO-1, CCG-2046, ellipticine, and PD 169316. The results demonstrated that both homogeneous TNF-α assays are robust and suitable for high throughput screening... - Drugs aimed at targeting characteristic karyotypic phenotypes of cancer cellsAnders Wallqvist
Science Applications International Corp, National Cancer Institute, NIH, Bethesda, MD, USA
Mol Cancer Ther 4:1559-68. 2005..We suggest that compounds identified in this study may represent new classes of potential anticancer agents...