Glen L Hortin

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Cross-reactivity of amino acids and other compounds in the biuret reaction: interference with urinary peptide measurements
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 51:1411-9. 2005
  2. ncbi request reprint Immunoassay interference by a commonly used blood collection tube additive, the organosilicone surfactant silwet L-720
    Raffick A R Bowen
    Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, Molecular Disease Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 51:1874-82. 2005
  3. doi request reprint The dynamic range problem in the analysis of the plasma proteome
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    J Proteomics 73:629-36. 2010
  4. doi request reprint Proteinuria without albuminuria: urinary protein excretion by a subset of patients with burn injuries
    Denis Sviridov
    Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda MD 20892, United States
    Clin Chim Acta 403:42-6. 2009
  5. ncbi request reprint Reactivity of urinary albumin (microalbumin) assays with fragmented or modified albumin
    Denis Sviridov
    Departments of Laboratory Medicine and Critical Care Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 54:61-8. 2008
  6. ncbi request reprint Quality control of serum albumin depletion for proteomic analysis
    Nitin Seam
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Clin Chem 53:1915-20. 2007
  7. ncbi request reprint Differential effect of blood collection tubes on total free fatty acids (FFA) and total triiodothyronine (TT3) concentration: a model for studying interference from tube constituents
    Raffick A R Bowen
    Clinical Chemistry Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chim Acta 378:181-93. 2007
  8. ncbi request reprint Proteomics: a new diagnostic frontier
    Glen L Hortin
    Department of Laboratory Medicine, Intramural research program of the NIH Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 52:1218-22. 2006
  9. doi request reprint High-abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypeptide abundance
    Glen L Hortin
    Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 54:1608-16. 2008
  10. ncbi request reprint Bound homocysteine, cysteine, and cysteinylglycine distribution between albumin and globulins
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 52:2258-64. 2006

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Cross-reactivity of amino acids and other compounds in the biuret reaction: interference with urinary peptide measurements
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 51:1411-9. 2005
    ..Some studies using biuret assays of urine suggest that small peptides generally are more abundant than proteins in urine, but it is not clear whether this is a problem of assay specificity...
  2. ncbi request reprint Immunoassay interference by a commonly used blood collection tube additive, the organosilicone surfactant silwet L-720
    Raffick A R Bowen
    Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, Molecular Disease Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 51:1874-82. 2005
    ..We examined the effect of a commonly used tube surfactant, Silwet L-720, on immunoassays and the mechanism for the interference...
  3. doi request reprint The dynamic range problem in the analysis of the plasma proteome
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    J Proteomics 73:629-36. 2010
    ..Thus, the dynamic range for these components is likely to greatly underestimate the total range of protein concentration in the plasma proteome...
  4. doi request reprint Proteinuria without albuminuria: urinary protein excretion by a subset of patients with burn injuries
    Denis Sviridov
    Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda MD 20892, United States
    Clin Chim Acta 403:42-6. 2009
    ..There is disagreement regarding the utility of urinary albumin excretion as a marker for capillary injury in patients with severe burn injuries. We examined protein components in urine specimens from patients with burn injury...
  5. ncbi request reprint Reactivity of urinary albumin (microalbumin) assays with fragmented or modified albumin
    Denis Sviridov
    Departments of Laboratory Medicine and Critical Care Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 54:61-8. 2008
    ..Controversy exists regarding occurrence and measurement of structural variants of albumin in urine. In this study, we examined cross-reactivity of in vitro modified albumins in assays for urine albumin (microalbumin)...
  6. ncbi request reprint Quality control of serum albumin depletion for proteomic analysis
    Nitin Seam
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Clin Chem 53:1915-20. 2007
    ..We examined the reproducibility of albumin immunodepletion and describe a method of QC for this process...
  7. ncbi request reprint Differential effect of blood collection tubes on total free fatty acids (FFA) and total triiodothyronine (TT3) concentration: a model for studying interference from tube constituents
    Raffick A R Bowen
    Clinical Chemistry Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chim Acta 378:181-93. 2007
    ..Besides total triiodothyronine (TT3), total free fatty acids (FFA) concentrations were higher with serum separator tube (SST) than Vacuette tubes...
  8. ncbi request reprint Proteomics: a new diagnostic frontier
    Glen L Hortin
    Department of Laboratory Medicine, Intramural research program of the NIH Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 52:1218-22. 2006
    ..Recent advances in technology and complete identification of the human genome sequence have opened up new opportunities for analysis of proteins for clinical diagnostic purposes...
  9. doi request reprint High-abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypeptide abundance
    Glen L Hortin
    Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 54:1608-16. 2008
    ..Plasma contains thousands of proteins, but a small number of these proteins comprise the majority of protein molecules and mass...
  10. ncbi request reprint Bound homocysteine, cysteine, and cysteinylglycine distribution between albumin and globulins
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chem 52:2258-64. 2006
    ..Major portions of homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly), and glutathione in serum are covalently bound to proteins via disulfides. Albumin has been considered the dominant binding protein...
  11. ncbi request reprint Diagnostic potential for urinary proteomics
    Glen L Hortin
    National Institutes of Health, Department of Laboratory Medicine, Warren Magnuson Clinical Center, Building 10, Room 2C 407, Bethesda, MD 20892 1508, USA
    Pharmacogenomics 8:237-55. 2007
    ..Examples of clinical situations where this may be of value are for more sensitive detection of kidney transplant rejection or of renal toxicity of medications...
  12. ncbi request reprint Coelution of other proteins with albumin during size-exclusion HPLC: Implications for analysis of urinary albumin
    Denis Sviridov
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1508, USA
    Clin Chem 52:389-97. 2006
    ..Size-exclusion HPLC has been used as an alternative to immunoassays for quantifying urinary albumin (microalbumin). Systematically higher values for the HPLC method have been proposed to result from nonimmunoreactive albumin...
  13. ncbi request reprint Indinavir-associated interstitial nephritis and urothelial inflammation: clinical and cytologic findings
    Jeffrey B Kopp
    Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA
    Clin Infect Dis 34:1122-8. 2002
    ....
  14. ncbi request reprint Substrate size selectivity of 20S proteasomes: analysis with variable-sized synthetic substrates
    Glen L Hortin
    J Protein Chem 21:333-7. 2002
    ..Macromolecular synthetic substrates appear to be better models of proteasome action on natural protein substrates and demonstrate substrate size selectivity of proteasomes...
  15. ncbi request reprint Parameters of coagulant and fibrinolytic capacity and activity in postmenopausal women: within-subject variability
    McDonald K Horne
    Department of Laboratory Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Thromb Res 107:229-33. 2002
    ..We observed large differences in within-subject variability among the tests and have demonstrated how such data can be used to estimate the number of times a parameter must be measured to produce a statistically adequate sample...
  16. ncbi request reprint Intraoperative parathyroid hormone testing: survey of testing program characteristics
    Glen L Hortin
    Department of Laboratory Medicine at the Warren Magnusson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Arch Pathol Lab Med 126:1045-9. 2002
    ..To examine the number and testing characteristics of laboratories that offer intraoperative testing of intact parathyroid hormone (PTH)...
  17. ncbi request reprint The MALDI-TOF mass spectrometric view of the plasma proteome and peptidome
    Glen L Hortin
    Department of Laboratory Medicine, National Institutes of Health, Bldg 10, Room 2C 407, Bethesda, MD 20892, USA
    Clin Chem 52:1223-37. 2006
    ....
  18. ncbi request reprint Effect of blood collection tubes on total triiodothyronine and other laboratory assays
    Raffick A R Bowen
    Clinical Chemistry Service, Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, NIH, Bethesda, MD 20892 1508, USA
    Clin Chem 51:424-33. 2005
    ..Increased total triiodothyronine (TT(3)) assay results in apparently euthyroid patients triggered an investigation of the effect of blood collection tubes on serum TT(3) and other laboratory assays...
  19. ncbi request reprint Potential interferences from blood collection tubes in mass spectrometric analyses of serum polypeptides
    Steven K Drake
    Departmentof Critical Care Medicine, Warren Magnuson Clinical Center, NIH, Bethesda, MD 20892, USA
    Clin Chem 50:2398-401. 2004
  20. pmc Diverse range of small peptides associated with high-density lipoprotein
    Glen L Hortin
    Department of Laboratory Medicine, National Institutes of Health, Building 10, Room 2C 407, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 340:909-15. 2006
    ..It is unclear whether the peptides have any significant functional role as apolipopeptides, but they may represent a pathway for peptide delivery or scavenging and a significant reservoir of plasma peptides for diagnostic evaluation...
  21. doi request reprint Urine albumin measurement: effects of urine matrix constituents
    Denis Sviridov
    Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Chim Acta 404:140-3. 2009
    ..There is increasing clinical interest in accurate measurement of urine albumin as an early indicator of kidney disease. However, urine is a highly variable sample matrix that may exert matrix effects on assays for urine albumin...
  22. doi request reprint Improved detection of intact tyrosine sulfate-containing peptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in linear negative ion mode
    Steven K Drake
    Department of Critical Care Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Biochem Cell Biol 42:174-9. 2010
    ..Dual analysis in positive and negative ion modes serves as a potential means of identifying peptides with labile modifications such as sulfation and distinguishing them from phosphorylation...
  23. pmc Pseudohyperphosphatemia associated with high-dose liposomal amphotericin B therapy
    Jason W Lane
    Microbiology Service, Department of Laboratory Medicine, W G Magnuson Clinical Center, NIH, Bethesda, Maryland, USA
    Clin Chim Acta 387:145-9. 2008
    ..75 mmol/l in the absence of hypocalcemia and tissue deposition of calcium phosphate were noted in 3 patients receiving liposomal amphotericin B (L-AMB). We investigated L-AMB as a possible cause of pseudohyperphosphatemia...
  24. ncbi request reprint Size-selective extraction of peptides from urine for mass spectrometric analysis
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, NIH, Bethesda, MD, USA
    Clin Chem 50:1092-5. 2004
  25. ncbi request reprint Oral glucosamine for 6 weeks at standard doses does not cause or worsen insulin resistance or endothelial dysfunction in lean or obese subjects
    Ranganath Muniyappa
    Chief, Diabetes Unit, National Center for Complementary and Alternative Medicine, National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6C 205, Bethesda, MD 20892, USA
    Diabetes 55:3142-50. 2006
    ..We conclude that oral glucosamine at standard doses for 6 weeks does not cause or significantly worsen insulin resistance or endothelial dysfunction in lean or obese subjects...
  26. doi request reprint Analysis of molecular forms of albumin in urine
    Glen L Hortin
    Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, MD, USA
    Proteomics Clin Appl 2:950-5. 2008
    ..Analysis of molecular forms of albumin in urine is of fundamental importance for understanding clinical assays for albumin quantification, specimen stability, and proteomic and peptidomic analysis of urine...
  27. ncbi request reprint A new era in protein quantification in clinical laboratories: application of liquid chromatography-tandem mass spectrometry
    Glen L Hortin
    Clin Chem 53:543-4. 2007
  28. ncbi request reprint Enrichment of low molecular weight fraction of serum for MS analysis of peptides associated with hepatocellular carcinoma
    Eduard Orvisky
    Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, Washington, DC 20057 1469, USA
    Proteomics 6:2895-902. 2006
    ..This study shows that enrichment of the low molecular weight fraction of serum facilitates an efficient discovery of peptides that could serve as biomarkers for detection of HCC as well as other diseases...
  29. ncbi request reprint Ant colony optimization for biomarker identification from MALDI-TOF mass spectra
    Habtom W Ressom
    Lombardi Comprehensive Cancer Center, Georgetown Univ, Washington, DC, USA
    Conf Proc IEEE Eng Med Biol Soc 1:4560-3. 2006
    ..The results indicate that the MALDI-TOF technology could provide the means to discover novel biomarkers for HCC...
  30. ncbi request reprint Can mass spectrometric protein profiling meet desired standards of clinical laboratory practice?
    Glen L Hortin
    Clin Chem 51:3-5. 2005
  31. ncbi request reprint Inflammatory protein profile during systemic high dose interleukin-2 administration
    Leonardo Rossi
    Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Italy
    Proteomics 6:709-20. 2006
    ..Overall distinct yet complementary information was obtained using different platforms, which may better illustrate complex phenomena such as the systemic response to biological response modifiers...
  32. ncbi request reprint New markers for kidney disease
    Robert Star
    Clin Chem 48:1375-6. 2002