Jason D Hoffert

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Taking aim at shotgun phosphoproteomics
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Anal Biochem 375:1-10. 2008
  2. pmc Phosphoproteomics of vasopressin signaling in the kidney
    Jason D Hoffert
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Expert Rev Proteomics 8:157-63. 2011
  3. pmc Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells
    Ruwan Gunaratne
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:15653-8. 2010
  4. pmc Vasopressin increases phosphorylation of Ser84 and Ser486 in Slc14a2 collecting duct urea transporters
    Shelly Hwang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Renal Physiol 299:F559-67. 2010
  5. pmc Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct
    Amar D Bansal
    National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 21:303-15. 2010
  6. pmc Quantitative analysis of aquaporin-2 phosphorylation
    Luke Xie
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 298:F1018-23. 2010
  7. pmc Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells
    Markus M Rinschen
    National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:3882-7. 2010
  8. pmc Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2
    Jason D Hoffert
    NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 283:24617-27. 2008
  9. pmc LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat
    Aaron N Sachs
    National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 295:F1799-806. 2008
  10. pmc Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells
    Sookkasem Khositseth
    Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Mol Cell Proteomics 10:M110.004036. 2011

Collaborators

  • Mark A Knepper
  • Robert A Fenton
  • Emily S Boja
  • Hanne B Moeller
  • Feng Chen
  • Primal de Lanerolle
  • Peter J Steinbach
  • Robert Kleta
  • Ryan G Morris
  • Trairak Pisitkun
  • Ming Jiun Yu
  • Chung Lin Chou
  • Fahad Saeed
  • Markus M Rinschen
  • Guanghui Wang
  • Pablo C Sandoval
  • Ruwan Gunaratne
  • Steven J Bolger
  • Boyang Zhao
  • Ewout J Hoorn
  • Shelly Hwang
  • Patricia A Gonzales
  • Dmitry Tchapyjnikov
  • J neka S Claxton
  • R Lance Miller
  • Jennifer C Huling
  • Dane H Slentz
  • Laura K Schenk
  • Jacqueline Douglass
  • Sookkasem Khositseth
  • Marina Feric
  • Amar D Bansal
  • Luke Xie
  • Nicholas A Zwang
  • Brian E Ruttenberg
  • Aaron N Sachs
  • Jakob Nielsen
  • Norman P Curthoys
  • Bas W M van Balkom
  • Gary Liu
  • Davis Bradford
  • Jae H Song
  • Kelli Luginbuhl
  • Patricia A Gonzales Hurtado
  • Yuedan Li
  • Drew W W Braucht
  • Nam Sun Wang
  • Robert A Star
  • Peter Agre
  • Susan M Wall
  • Søren Nielsen
  • Eliza M Kassai
  • Lynn Taylor
  • Rong Fong Shen
  • Wells W Wu
  • Henrik Vorum
  • Ravi A Desai
  • Soren Nielsen
  • Sebastian Frische
  • Birgitte M Christensen

Detail Information

Publications41

  1. pmc Taking aim at shotgun phosphoproteomics
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Anal Biochem 375:1-10. 2008
  2. pmc Phosphoproteomics of vasopressin signaling in the kidney
    Jason D Hoffert
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
    Expert Rev Proteomics 8:157-63. 2011
    ....
  3. pmc Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells
    Ruwan Gunaratne
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:15653-8. 2010
    ..These results support the view that, although protein kinase A plays a central role in mTAL signaling, additional kinases, including those that target proline-directed motifs, may be involved...
  4. pmc Vasopressin increases phosphorylation of Ser84 and Ser486 in Slc14a2 collecting duct urea transporters
    Shelly Hwang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Renal Physiol 299:F559-67. 2010
    ..The findings add to prior evidence for vasopressin-induced phosphorylation of UT-A1, providing evidence that UT-A3 may be regulated by phosphorylation as well...
  5. pmc Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting duct
    Amar D Bansal
    National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 21:303-15. 2010
    ..These results expand the known list of collecting duct phosphoproteins and highlight the utility of targeted phosphoproteomic approaches...
  6. pmc Quantitative analysis of aquaporin-2 phosphorylation
    Luke Xie
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 298:F1018-23. 2010
    ..We suggest from these studies that Ser269 phosphorylation may be a more consistent indicator of vasopressin action and AQP2 membrane abundance than is Ser256 phosphorylation...
  7. pmc Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells
    Markus M Rinschen
    National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:3882-7. 2010
    ....
  8. pmc Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2
    Jason D Hoffert
    NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 283:24617-27. 2008
    ..The data support a model in which vasopressin-mediated phosphorylation of AQP2 at Ser269:(a) depends on prior PKA-mediated phosphorylation of Ser256 and (b) enhances apical plasma membrane retention of AQP2...
  9. pmc LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat
    Aaron N Sachs
    National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 295:F1799-806. 2008
    ....
  10. pmc Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells
    Sookkasem Khositseth
    Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Mol Cell Proteomics 10:M110.004036. 2011
    ..The results provide impetus for increased focus on translational regulation and regulation of protein degradation in physiological control in mammalian epithelial cells...
  11. pmc Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomics
    Jason D Hoffert
    Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 11:M111.014613. 2012
    ..Finally, we provide an online resource of physiologically regulated phosphorylation sites with dynamic quantitative data (http://helixweb.nih.gov/ESBL/Database/TiPD/index.html)...
  12. pmc An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, Proteomics Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 6:3501-8. 2007
    ..Using a label-free quantification approach, we identified phosphoproteins that changed in abundance with vasopressin exposure including aquaporin-2 (AQP2), Hnrpa3, IP3 receptor 3, and pur-beta...
  13. pmc Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MS
    Dmitry Tchapyjnikov
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Physiol Genomics 40:167-83. 2010
    ..The newly identified proteins have been incorporated into the IMCD Proteome Database (http://dir.nhlbi.nih.gov/papers/lkem/imcd/)...
  14. doi request reprint CPhos: a program to calculate and visualize evolutionarily conserved functional phosphorylation sites
    Boyang Zhao
    National Heart, Lung, and Blood Institute, Epithelial Systems Biology Laboratory, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Proteomics 12:3299-303. 2012
    ..CPhos can be accessed or downloaded at http://helixweb.nih.gov/CPhos/...
  15. pmc Quantitative proteomics identifies vasopressin-responsive nuclear proteins in collecting duct cells
    Laura K Schenk
    National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1603, USA
    J Am Soc Nephrol 23:1008-18. 2012
    ..The findings provide a new online database resource for nuclear proteomics (http://helixweb.nih.gov/ESBL/Database/mNPD/) and generate new hypotheses regarding vasopressin-mediated transcriptional regulation in the collecting duct...
  16. pmc Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry
    Nicholas A Zwang
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 8:1540-54. 2009
    ..These results suggest that phosphorylation of AQP2 at Ser-256 may regulate AQP2 trafficking in part by mediating differential binding of hsp70 family proteins to the COOH-terminal tail...
  17. ncbi request reprint Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSn
    Guanghui Wang
    Proteomics Core Facility and Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Chem 78:5752-61. 2006
    ..Compared with a widely used commercial software tool, QUIL showed improvement in ion chromatogram construction and peak integration and significantly reduced relative errors in abundance ratio assessment...
  18. pmc PhosphoScore: an open-source phosphorylation site assignment tool for MSn data
    Brian E Ruttenberg
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 7:3054-9. 2008
    ..PhosphoScore produced >95% correct MS(2) assignments from known synthetic data, > 98% agreement with an established MS(2) assignment algorithm (Ascore), and >92% agreement with visual inspection of MS(3) and MS(4) spectra...
  19. pmc Large-scale proteomics and phosphoproteomics of urinary exosomes
    Patricia A Gonzales
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Am Soc Nephrol 20:363-79. 2009
    ..The proteomic data are publicly accessible at http://dir.nhlbi.nih.gov/papers/lkem/exosome/...
  20. ncbi request reprint Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 292:F691-700. 2007
    ....
  21. pmc Quantitative phosphoproteomics in nuclei of vasopressin-sensitive renal collecting duct cells
    Steven J Bolger
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Cell Physiol 303:C1006-20. 2012
    ..All data are available to users at http://helixweb.nih.gov/ESBL/Database/mNPPD/...
  22. pmc Identifying protein kinase target preferences using mass spectrometry
    Jacqueline Douglass
    National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Cell Physiol 303:C715-27. 2012
    ....
  23. pmc Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites
    Jason D Hoffert
    National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:7159-64. 2006
    ....
  24. ncbi request reprint Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro rat
    Bas W M van Balkom
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 286:F216-24. 2004
    ....
  25. pmc Proteome-wide measurement of protein half-lives and translation rates in vasopressin-sensitive collecting duct cells
    Pablo C Sandoval
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
    J Am Soc Nephrol 24:1793-805. 2013
    ..nih.gov/ESBL/Database/ProteinHalfLives/index.html). ..
  26. pmc Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct
    Chung Lin Chou
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 295:F192-201. 2008
    ..We conclude that the AVP-induced cell height increase is dependent on basolateral solute uptake via NKCC1 and changes in actin organization via myosin II, but is not dependent specifically on increased apical water entry...
  27. ncbi request reprint Tandem mass spectrometry in physiology
    Trairak Pisitkun
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Physiology (Bethesda) 22:390-400. 2007
    ..Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology...
  28. pmc Vasopressin inhibits apoptosis in renal collecting duct cells
    R Lance Miller
    Eptihelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Renal Physiol 304:F177-88. 2013
    ..The antiapoptotic action of AVP may be relevant to a number of physiological and pathophysiological conditions including osmotic tolerance in the inner medulla, escape from AVP-induced antidiuresis, and polycystic kidney disease...
  29. pmc Gene expression databases for kidney epithelial cells
    Jennifer C Huling
    National Institutes of Health 10 Center Dr Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 302:F401-7. 2012
    ..Here, we provide a roadmap to these databases and illustrate how they may be useful in the design and interpretation of physiological studies. The databases can be accessed through http://helixweb.nih.gov/ESBL/Database...
  30. pmc Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule
    Marina Feric
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Cell Physiol 300:C755-70. 2011
    ..An online database from this study (http://dir.nhlbi.nih.gov/papers/lkem/rcmpd/) provides a resource for future studies of transporter regulation...
  31. ncbi request reprint Non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting duct
    Chung Lin Chou
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 279:49026-35. 2004
    ....
  32. pmc NHLBI-AbDesigner: an online tool for design of peptide-directed antibodies
    Trairak Pisitkun
    Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    Am J Physiol Cell Physiol 302:C154-64. 2012
    ....
  33. pmc Combined proteomics and pathways analysis of collecting duct reveals a protein regulatory network activated in vasopressin escape
    Ewout J Hoorn
    National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892, USA
    J Am Soc Nephrol 16:2852-63. 2005
    ..The results demonstrate a dominant protein regulatory network in IMCD cells that is altered in association with vasopressin escape, providing a new framework for further studies of signaling in IMCD...
  34. ncbi request reprint The application of DIGE-based proteomics to renal physiology
    Ewout J Hoorn
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nephron Physiol 104:p61-72. 2006
    ..Finally, we illustrate how quantification based on the DIGE approach combined with bioinformatics may facilitate the study of systems biology of the kidney...
  35. pmc Calmodulin is required for vasopressin-stimulated increase in cyclic AMP production in inner medullary collecting duct
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:13624-30. 2005
    ..These studies demonstrate the importance of calmodulin in the regulation of collecting duct adenylyl cyclase activity and transport function...
  36. pmc Aquaporin-2 in the "-omics" era
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
    J Biol Chem 284:14683-7. 2009
    ..Here we review this progress...
  37. pmc Endogenous carbamylation of renal medullary proteins
    J neka S Claxton
    Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e82655. 2013
    ..nih.gov/ESBL/Database/Carbamylation/Carbamylation_peptide_sorted.html]. We conclude that protein carbamylation occurs endogenously in the kidney, modifying many physiologically important proteins. ..
  38. doi request reprint Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional alleles
    Jason D Hoffert
    Epithelial Systems Biology Group, Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, 6N312, Bethesda, MD 20892, USA
    Transgenic Res 21:665-9. 2012
    ..We provide a description of CAMP, how to use it, and offer it freely as an application...
  39. ncbi request reprint Application of difference gel electrophoresis to the identification of inner medullary collecting duct proteins
    Jason D Hoffert
    Laboratory of Kidney and Electrolyte Mechanism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10, Rm 6N260, MSC 1603, 10 Center Drive, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 286:F170-9. 2004
    ..These studies document the applicability of a standardized approach to purification of IMCD cells for proteomic analysis of IMCD proteins and demonstrate the feasibility of large scale identification of proteins in the native IMCD cell...
  40. ncbi request reprint Proteomic analysis of the adaptive response of rat renal proximal tubules to metabolic acidosis
    Norman P Curthoys
    Dept of Biochemistry and Molecular Biology, Colorado State Univ, Campus Delivery 1870, Fort Collins, CO 80523 1870, USA
    Am J Physiol Renal Physiol 292:F140-7. 2007
    ..Thus selective mRNA stabilization may be a predominant mechanism by which protein expression is increased in response to acidosis...
  41. pmc Proteomic analysis of lithium-induced nephrogenic diabetes insipidus: mechanisms for aquaporin 2 down-regulation and cellular proliferation
    Jakob Nielsen
    Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
    Proc Natl Acad Sci U S A 105:3634-9. 2008
    ..This study provides a comprehensive analysis of the proteins affected by lithium treatment in the IMCD and, as such, provides clues to potential lithium targets in the brain...