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Genomes and Genes | Jason D HoffertSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Taking aim at shotgun phosphoproteomicsJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Anal Biochem 375:1-10. 2008- Phosphoproteomics of vasopressin signaling in the kidneyJason D Hoffert
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, Bethesda, MD 20892, USA
Expert Rev Proteomics 8:157-63. 2011.... - Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cellsRuwan Gunaratne
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:15653-8. 2010..These results support the view that, although protein kinase A plays a central role in mTAL signaling, additional kinases, including those that target proline-directed motifs, may be involved... - Vasopressin increases phosphorylation of Ser84 and Ser486 in Slc14a2 collecting duct urea transportersShelly Hwang
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Renal Physiol 299:F559-67. 2010..The findings add to prior evidence for vasopressin-induced phosphorylation of UT-A1, providing evidence that UT-A3 may be regulated by phosphorylation as well... - Phosphoproteomic profiling reveals vasopressin-regulated phosphorylation sites in collecting ductAmar D Bansal
National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 21:303-15. 2010..These results expand the known list of collecting duct phosphoproteins and highlight the utility of targeted phosphoproteomic approaches... - Quantitative analysis of aquaporin-2 phosphorylationLuke Xie
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Am J Physiol Renal Physiol 298:F1018-23. 2010..We suggest from these studies that Ser269 phosphorylation may be a more consistent indicator of vasopressin action and AQP2 membrane abundance than is Ser256 phosphorylation... - Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cellsMarkus M Rinschen
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:3882-7. 2010.... - Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2Jason D Hoffert
NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 283:24617-27. 2008..The data support a model in which vasopressin-mediated phosphorylation of AQP2 at Ser269:(a) depends on prior PKA-mediated phosphorylation of Ser256 and (b) enhances apical plasma membrane retention of AQP2... - Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cellsSookkasem Khositseth
Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, MD 20892 1603, USA
Mol Cell Proteomics 10:M110.004036. 2011..The results provide impetus for increased focus on translational regulation and regulation of protein degradation in physiological control in mammalian epithelial cells... - LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of ratAaron N Sachs
National Institutes of Health, 10 Center Dr, Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 295:F1799-806. 2008.... - Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomicsJason D Hoffert
Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Proteomics 11:M111.014613. 2012..Finally, we provide an online resource of physiologically regulated phosphorylation sites with dynamic quantitative data (http://helixweb.nih.gov/ESBL/Database/TiPD/index.html)... - An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteinsJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, Proteomics Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
J Proteome Res 6:3501-8. 2007..Using a label-free quantification approach, we identified phosphoproteins that changed in abundance with vasopressin exposure including aquaporin-2 (AQP2), Hnrpa3, IP3 receptor 3, and pur-beta... - Proteomic profiling of nuclei from native renal inner medullary collecting duct cells using LC-MS/MSDmitry Tchapyjnikov
Epithelial Systems Biology Laboratory, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Physiol Genomics 40:167-83. 2010..The newly identified proteins have been incorporated into the IMCD Proteome Database (http://dir.nhlbi.nih.gov/papers/lkem/imcd/)... - Quantitative proteomics identifies vasopressin-responsive nuclear proteins in collecting duct cellsLaura K Schenk
National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1603, USA
J Am Soc Nephrol 23:1008-18. 2012..The findings provide a new online database resource for nuclear proteomics (http://helixweb.nih.gov/ESBL/Database/mNPD/) and generate new hypotheses regarding vasopressin-mediated transcriptional regulation in the collecting duct... - Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometryNicholas A Zwang
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Proteome Res 8:1540-54. 2009..These results suggest that phosphorylation of AQP2 at Ser-256 may regulate AQP2 trafficking in part by mediating differential binding of hsp70 family proteins to the COOH-terminal tail... 
Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSnGuanghui Wang
Proteomics Core Facility and Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Chem 78:5752-61. 2006..Compared with a widely used commercial software tool, QUIL showed improvement in ion chromatogram construction and peak integration and significantly reduced relative errors in abundance ratio assessment...- PhosphoScore: an open-source phosphorylation site assignment tool for MSn dataBrian E Ruttenberg
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
J Proteome Res 7:3054-9. 2008..PhosphoScore produced >95% correct MS(2) assignments from known synthetic data, > 98% agreement with an established MS(2) assignment algorithm (Ascore), and >92% agreement with visual inspection of MS(3) and MS(4) spectra... - Large-scale proteomics and phosphoproteomics of urinary exosomesPatricia A Gonzales
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Am Soc Nephrol 20:363-79. 2009..The proteomic data are publicly accessible at http://dir.nhlbi.nih.gov/papers/lkem/exosome/... - Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting ductChung Lin Chou
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Am J Physiol Renal Physiol 295:F192-201. 2008..We conclude that the AVP-induced cell height increase is dependent on basolateral solute uptake via NKCC1 and changes in actin organization via myosin II, but is not dependent specifically on increased apical water entry... - Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting ductJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 292:F691-700. 2007.... 
Identifying protein kinase target preferences using mass spectrometryJacqueline Douglass
National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Cell Physiol 303:C715-27. 2012....- Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sitesJason D Hoffert
National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:7159-64. 2006.... - Tandem mass spectrometry in physiologyTrairak Pisitkun
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Physiology (Bethesda) 22:390-400. 2007..Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology... - Proteomic analysis of long-term vasopressin action in the inner medullary collecting duct of the Brattleboro ratBas W M van Balkom
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 286:F216-24. 2004.... - Gene expression databases for kidney epithelial cellsJennifer C Huling
National Institutes of Health 10 Center Dr Bldg 10, Rm 6N260, Bethesda, MD 20892 1603, USA
Am J Physiol Renal Physiol 302:F401-7. 2012..Here, we provide a roadmap to these databases and illustrate how they may be useful in the design and interpretation of physiological studies. The databases can be accessed through http://helixweb.nih.gov/ESBL/Database... - Non-muscle myosin II and myosin light chain kinase are downstream targets for vasopressin signaling in the renal collecting ductChung Lin Chou
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 279:49026-35. 2004.... - Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubuleMarina Feric
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Cell Physiol 300:C755-70. 2011..An online database from this study (http://dir.nhlbi.nih.gov/papers/lkem/rcmpd/) provides a resource for future studies of transporter regulation... - NHLBI-AbDesigner: an online tool for design of peptide-directed antibodiesTrairak Pisitkun
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
Am J Physiol Cell Physiol 302:C154-64. 2012.... - Combined proteomics and pathways analysis of collecting duct reveals a protein regulatory network activated in vasopressin escapeEwout J Hoorn
National Institutes of Health, 10 Center Drive, Building 10, Room 6N260, Bethesda, MD 20892, USA
J Am Soc Nephrol 16:2852-63. 2005..The results demonstrate a dominant protein regulatory network in IMCD cells that is altered in association with vasopressin escape, providing a new framework for further studies of signaling in IMCD... - CPhos: a program to calculate and visualize evolutionarily conserved functional phosphorylation sitesBoyang Zhao
National Heart, Lung, and Blood Institute, Epithelial Systems Biology Laboratory, National Institutes of Health, Bethesda, MD 20892 1603, USA
Proteomics 12:3299-303. 2012..CPhos can be accessed or downloaded at http://helixweb.nih.gov/CPhos/... - Aquaporin-2 in the "-omics" eraJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1603, USA
J Biol Chem 284:14683-7. 2009..Here we review this progress... - The application of DIGE-based proteomics to renal physiologyEwout J Hoorn
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. 20892, USA
Nephron Physiol 104:p61-72. 2006..Finally, we illustrate how quantification based on the DIGE approach combined with bioinformatics may facilitate the study of systems biology of the kidney... - Calmodulin is required for vasopressin-stimulated increase in cyclic AMP production in inner medullary collecting ductJason D Hoffert
Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 280:13624-30. 2005..These studies demonstrate the importance of calmodulin in the regulation of collecting duct adenylyl cyclase activity and transport function... - Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional allelesJason D Hoffert
Epithelial Systems Biology Group, Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, 6N312, Bethesda, MD 20892, USA
Transgenic Res 21:665-9. 2012..We provide a description of CAMP, how to use it, and offer it freely as an application... - Application of difference gel electrophoresis to the identification of inner medullary collecting duct proteinsJason D Hoffert
Laboratory of Kidney and Electrolyte Mechanism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Rm. 6N260, MSC 1603, 10 Center Drive, Bethesda, MD 20892-1603, USA
Am J Physiol Renal Physiol 286:F170-9. 2004..These studies document the applicability of a standardized approach to purification of IMCD cells for proteomic analysis of IMCD proteins and demonstrate the feasibility of large scale identification of proteins in the native IMCD cell... - Proteomic analysis of lithium-induced nephrogenic diabetes insipidus: mechanisms for aquaporin 2 down-regulation and cellular proliferationJakob Nielsen
Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
Proc Natl Acad Sci U S A 105:3634-9. 2008..This study provides a comprehensive analysis of the proteins affected by lithium treatment in the IMCD and, as such, provides clues to potential lithium targets in the brain... - Proteomic analysis of the adaptive response of rat renal proximal tubules to metabolic acidosisNorman P Curthoys
Dept of Biochemistry and Molecular Biology, Colorado State Univ, Campus Delivery 1870, Fort Collins, CO 80523 1870, USA
Am J Physiol Renal Physiol 292:F140-7. 2007..Thus selective mRNA stabilization may be a predominant mechanism by which protein expression is increased in response to acidosis...