Alan G Hinnebusch

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Molecular mechanism of scanning and start codon selection in eukaryotes
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Microbiol Mol Biol Rev 75:434-67, first page of table of contents. 2011
  2. ncbi request reprint eIF3: a versatile scaffold for translation initiation complexes
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Biochem Sci 31:553-62. 2006
  3. ncbi request reprint Study of translational control of eukaryotic gene expression using yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Division of Intramural Research, NICHD, NIH, Bldg 18T, Rm 106, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 1038:60-74. 2004
  4. pmc Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection
    LEOS VALASEK
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 24:9437-55. 2004
  5. pmc The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons
    Wen Ling Chiu
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:4415-34. 2010
  6. pmc A triad of subunits from the Gal11/tail domain of Srb mediator is an in vivo target of transcriptional activator Gcn4p
    Fan Zhang
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Building 6A Room B1A13, Bethesda, MD 20892, USA
    Mol Cell Biol 24:6871-86. 2004
  7. pmc Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast
    Klaus H Nielsen
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 6A Rm B1A 13, Bethesda, MD 20892, USA
    Mol Cell Biol 26:2984-98. 2006
  8. pmc Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo
    Chhabi K Govind
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 25:5626-38. 2005
  9. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
  10. pmc Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo
    Soon ja Kim
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell Biol 25:11171-83. 2005

Collaborators

Detail Information

Publications76

  1. pmc Molecular mechanism of scanning and start codon selection in eukaryotes
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Microbiol Mol Biol Rev 75:434-67, first page of table of contents. 2011
    ....
  2. ncbi request reprint eIF3: a versatile scaffold for translation initiation complexes
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Biochem Sci 31:553-62. 2006
    ..This location of eIF3 seems ideally suited for its other proposed regulatory functions, including reinitiating translation on polycistronic mRNAs and acting as a receptor for protein kinases that control protein synthesis...
  3. ncbi request reprint Study of translational control of eukaryotic gene expression using yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Division of Intramural Research, NICHD, NIH, Bldg 18T, Rm 106, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 1038:60-74. 2004
    ..Thus, apart from its critical role in the starvation response, GCN4 regulation is a valuable tool for dissecting the contributions of multiple translation factors in the eukaryotic initiation pathway...
  4. pmc Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection
    LEOS VALASEK
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 24:9437-55. 2004
    ..Thus, the NIP1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection of AUG start codons in vivo...
  5. pmc The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons
    Wen Ling Chiu
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:4415-34. 2010
    ....
  6. pmc A triad of subunits from the Gal11/tail domain of Srb mediator is an in vivo target of transcriptional activator Gcn4p
    Fan Zhang
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Building 6A Room B1A13, Bethesda, MD 20892, USA
    Mol Cell Biol 24:6871-86. 2004
    ..Hence, the Gal11p/Med2p/Pgd1p triad may contribute to TBP recruitment independently of the rest of mediator...
  7. pmc Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast
    Klaus H Nielsen
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 6A Rm B1A 13, Bethesda, MD 20892, USA
    Mol Cell Biol 26:2984-98. 2006
    ..Thus, the PRT1 RNP1 motif coordinates the functions of HCR1 and TIF32 in 40S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vivo...
  8. pmc Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo
    Chhabi K Govind
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 25:5626-38. 2005
    ..Our findings reveal a program of coactivator recruitment and PIC assembly that distinguishes Gcn4p from other yeast activators studied thus far...
  9. pmc The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo
    Kamal Dev
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Mol Cell Biol 30:5218-33. 2010
    ..In aggregate, these findings provide compelling evidence that β/Gcd7 is crucial for binding of substrate by eIF2B in vivo, beyond its dispensable regulatory role in the inhibition of eIF2B by eIF (αP)...
  10. pmc Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo
    Soon ja Kim
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell Biol 25:11171-83. 2005
    ..Our findings reveal a novel coactivator function for Cyc8p/Tup1p at the level of activator binding and suggest that Gcn4p may enhance its own binding to the UAS by recruiting Cyc8p/Tup1p...
  11. pmc Yeast eukaryotic initiation factor 4B (eIF4B) enhances complex assembly between eIF4A and eIF4G in vivo
    Eun Hee Park
    Laboratory of Gene Regulation and Development, Eunice K Shriver NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:2340-54. 2013
    ..These and other findings lead us to propose that yeIF4B acts in vivo to promote eIF4F assembly by enhancing a conformation of the HEAT domain of yeast eIF4G conducive for stable binding to eIF4A...
  12. pmc Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo
    Iness Jedidi
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:2438-55. 2010
    ..Gcn4 is distinctive in relying on comparable contributions from multiple segments of Gal11 for efficient recruitment of Mediator in vivo...
  13. pmc Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes
    Chhabi K Govind
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 39:234-46. 2010
    ..A strong correlation between increased acetylation and lower histone occupancy in HDA mutants implies that histone acetylation is important for nucleosome eviction...
  14. pmc Recruitment of the ArgR/Mcm1p repressor is stimulated by the activator Gcn4p: a self-checking activation mechanism
    Sungpil Yoon
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:11713-8. 2004
    ..By recruiting an arginine-regulated repressor, Gcn4p can precisely modulate its activation function at ARG1 according to the availability of arginine...
  15. pmc Functions of eIF3 downstream of 48S assembly impact AUG recognition and GCN4 translational control
    Klaus H Nielsen
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892 2716, USA
    EMBO J 23:1166-77. 2004
    ..Other mutations that disrupt eIF2-eIF3 contacts in the multifactor complex (MFC) diminished 40S-bound TC, indicating that MFC formation enhances 43S assembly in vivo...
  16. pmc The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo
    LEOS VALASEK
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development NIH, Bethesda, MD 20892, USA
    Genes Dev 17:786-99. 2003
    ....
  17. pmc Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development NIH, Building 6A, Bethesda, MD 20892, USA
    Mol Cell Biol 25:3461-74. 2005
    ..Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo...
  18. pmc The novel ATP-binding cassette protein ARB1 is a shuttling factor that stimulates 40S and 60S ribosome biogenesis
    Jinsheng Dong
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 25:9859-73. 2005
    ..Mutations of conserved ARB1 residues expected to function in ATP hydrolysis were lethal. We propose that ARB1 functions as a mechanochemical ATPase to stimulate multiple steps in the 40S and 60S ribosomal biogenesis pathways...
  19. pmc Eukaryotic translation initiation factor 3 (eIF3) and eIF2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast
    Antonina V Jivotovskaya
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Building 6A Room B1A13, Bethesda, MD 20892, USA
    Mol Cell Biol 26:1355-72. 2006
    ....
  20. pmc Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4
    Fan Zhang
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 28:6796-818. 2008
    ....
  21. pmc Vps factors are required for efficient transcription elongation in budding yeast
    Naseem A Gaur
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Genetics 193:829-51. 2013
    ..These findings suggest that Vps factors enhance the efficiency of transcription elongation in a manner involving their physical proximity to nuclear pores and transcribed chromatin...
  22. pmc N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection
    Christie A Fekete
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 26:1602-14. 2007
    ..We conclude that tight binding of eIF1A to the PIC is an important determinant of AUG selection and is modulated in opposite directions by residues in the NTT and CTT of eIF1A...
  23. pmc Snf1 promotes phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 by activating Gcn2 and inhibiting phosphatases Glc7 and Sit4
    Vera Cherkasova
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Mol Cell Biol 30:2862-73. 2010
    ..Our results show that Snf1 modulates the level of eIF2alpha phosphorylation by different mechanisms, depending on the kind of nutrient deprivation existing in cells...
  24. pmc An array of coactivators is required for optimal recruitment of TATA binding protein and RNA polymerase II by promoter-bound Gcn4p
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 24:4104-17. 2004
    ..Our results reveal an unexpected complexity in the cofactor requirements for the enhancement of PIC assembly by a single activator protein...
  25. pmc A network of hydrophobic residues impeding helix alphaC rotation maintains latency of kinase Gcn2, which phosphorylates the alpha subunit of translation initiation factor 2
    Andrés Gárriz
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 29:1592-607. 2009
    ....
  26. pmc Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome
    Adesh K Saini
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 24:97-110. 2010
    ....
  27. pmc Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo
    Yuen Nei Cheung
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 21:1217-30. 2007
    ....
  28. pmc Recruitment of SWI/SNF by Gcn4p does not require Snf2p or Gcn5p but depends strongly on SWI/SNF integrity, SRB mediator, and SAGA
    Sungpil Yoon
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Mol Cell Biol 23:8829-45. 2003
    ..We suggest that SWI/SNF recruitment is enhanced by cooperative interactions with subunits of SRB mediator and SAGA recruited by Gcn4p to the same promoter but is insensitive to histone H3 acetylation by Gcn5p...
  29. pmc NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5
    Daniel S Ginsburg
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Cell Biol 29:6473-87. 2009
    ..Our findings delineate a pathway for acetylation-mediated nucleosome remodeling and eviction in coding sequences that stimulates transcription elongation by Pol II in vivo...
  30. ncbi request reprint YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed
    Evelyn Sattlegger
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892 2427, USA
    J Biol Chem 279:29952-62. 2004
    ....
  31. pmc Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA
    EMBO J 31:3494-505. 2012
    ..We propose that pCTD repeats and Spt5 pCTRs provide separate interaction surfaces that cooperate to ensure high-level Paf1C recruitment...
  32. pmc Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection
    Jinsheng Dong
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    Genes Dev 22:2242-55. 2008
    ..Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA(i)(Met) binding and AUG selection in eukaryotes...
  33. ncbi request reprint Gcn5 promotes acetylation, eviction, and methylation of nucleosomes in transcribed coding regions
    Chhabi K Govind
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 25:31-42. 2007
    ..Gcn5p also enhances H3-K4 trimethylation in the ARG1 ORF and bulk histones. Thus, Gcn5p, most likely in SAGA, stimulates modification and eviction of nucleosomes in transcribed coding sequences and promotes Pol II elongation...
  34. pmc Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo
    DeAnne S Olsen
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    EMBO J 22:193-204. 2003
    ..We propose a modular organization for eIF1A wherein a core ribosome-binding domain is flanked by flexible segments that mediate interactions with other factors involved in recruitment of TC and release of eIF1A at subunit joining...
  35. pmc Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons
    Pilar Martin-Marcos
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 31:4814-31. 2011
    ..Although eIF1 autoregulates by discriminating against poor context in yeast and mammals, this mechanism does not prevent eIF1 overproduction in yeast, accounting for the hyperaccuracy phenotype afforded by SUI1 overexpression...
  36. pmc Phosphorylation of the Pol II CTD by KIN28 enhances BUR1/BUR2 recruitment and Ser2 CTD phosphorylation near promoters
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 33:752-62. 2009
    ..By contrast, CTK1 is responsible for the bulk of Ser2P in total Pol II and at promoter-distal sites. In addition to phosphorylating Ser2 near promoters, BUR1/BUR2 also stimulates Ser2P formation by CTK1 during transcription elongation...
  37. pmc A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo
    Mark J Swanson
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 23:2800-20. 2003
    ..We observed considerable differences in coactivator requirements among several Gcn4p-dependent promoters; thus, only a subset of the array of coactivators that can be recruited by Gcn4p is required at a given target gene in vivo...
  38. ncbi request reprint The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly
    Jinsheng Dong
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:42157-68. 2004
    ..Thus, RLI1 may have dual functions in translation initiation and ribosome biogenesis...
  39. pmc The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 6A Rm B1A 13, Bethesda, MD 20892, USA
    Mol Cell Biol 26:3135-48. 2006
    ..spt4Delta reduces trimethylation of Lys4 on histone H3, demonstrating a new role for yeast DSIF in promoting a Paf1C-dependent function in elongation...
  40. pmc Identification and characterization of functionally critical, conserved motifs in the internal repeats and N-terminal domain of yeast translation initiation factor 4B (yeIF4B)
    Fujun Zhou
    From the Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 and
    J Biol Chem 289:1704-22. 2014
    ..These results provide a comprehensive description of the critical sequence elements in yeIF4B that support eIF4F function in mRNA recruitment by the PIC. ..
  41. pmc Yeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sites
    Chi Ming Wong
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Mol Cell Biol 27:6520-31. 2007
    ....
  42. ncbi request reprint GCN2 whets the appetite for amino acids
    Thomas E Dever
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 18:141-2. 2005
    ..Two recent studies (Maurin et al., 2005; Hao et al., 2005) reveal that GCN2 in the brain of mice restricts intake of diets lacking essential amino acids...
  43. ncbi request reprint Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation
    Evelyn Sattlegger
    Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:16514-21. 2005
    ..This conclusion supports the model that an eEF3-related activity of GCN1 influences occupancy of the ribosomal decoding site by uncharged tRNA in starved cells...
  44. ncbi request reprint The essential vertebrate ABCE1 protein interacts with eukaryotic initiation factors
    Zhang qun Chen
    Basic Research Program, SAIC Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    J Biol Chem 281:7452-7. 2006
    ..The evolutionary analysis supports a model in which an ancestral eukaryote had large number of introns and that many of these introns were lost in non-vertebrate lineages...
  45. pmc Conserved residues in yeast initiator tRNA calibrate initiation accuracy by regulating preinitiation complex stability at the start codon
    Jinsheng Dong
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 28:502-20. 2014
    ....
  46. pmc Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1•PABP mRNPs in vivo
    Eun Hee Park
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    EMBO J 30:302-16. 2011
    ..Interestingly, two other RNA-binding regions in eIF4G1 have critical functions downstream of eIF4F•mRNA assembly...
  47. pmc The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo
    Christie A Fekete
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    EMBO J 24:3588-601. 2005
    ..Thus, the OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in TC recruitment and scanning...
  48. ncbi request reprint Translational regulation of GCN4 and the general amino acid control of yeast
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 59:407-50. 2005
    ..Remarkably, mammalian cells use the same strategy to downregulate protein synthesis while inducing transcriptional activators of stress-response genes under various stressful conditions, including amino acid starvation...
  49. pmc Archaeal aIF2B interacts with eukaryotic translation initiation factors eIF2alpha and eIF2Balpha: Implications for aIF2B function and eIF2B regulation
    Kamal Dev
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Mol Biol 392:701-22. 2009
    ..In this model, the evolutionarily conserved regions and sites of regulatory mutations in the three eIF2B subunits in yeast are juxtaposed in one continuous binding surface for phosphorylated eIF2alpha...
  50. pmc Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing
    Ka Yiu Edwin Kong
    Department of Biochemistry, Department of Medicine, State Key Laboratory of Pharmaceutical Biotechnology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong and Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 42:643-60. 2014
    ....
  51. pmc β-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo
    Pilar Martin-Marcos
    From the Laboratory of Gene Regulation and Development, Eunice K Shriver NICHD, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 288:27546-62. 2013
    ..Finally, we implicate the unstructured N-terminal tail of eIF1 in blocking rearrangement to the closed conformation in the scanning preinitiation complex. ..
  52. pmc An upstream ORF with non-AUG start codon is translated in vivo but dispensable for translational control of GCN4 mRNA
    Fan Zhang
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 39:3128-40. 2011
    ..Thus, although nAuORF2 is translated in vivo, it appears that this event is not stimulated by eIF2α phosphorylation nor significantly influences GCN4 translational induction under various starvation or stress conditions...
  53. pmc Guanine nucleotide pool imbalance impairs multiple steps of protein synthesis and disrupts GCN4 translational control in Saccharomyces cerevisiae
    Diego Iglesias-Gato
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genetics 187:105-22. 2011
    ....
  54. pmc Translational control by TOR and TAP42 through dephosphorylation of eIF2alpha kinase GCN2
    Vera A Cherkasova
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Genes Dev 17:859-72. 2003
    ..Our results add a new dimension to the regulation of protein synthesis by TOR proteins and demonstrate cross-talk between two major pathways for nutrient control of gene expression in yeast...
  55. pmc Depletion of eIF4G from yeast cells narrows the range of translational efficiencies genome-wide
    Eun Hee Park
    Laboratory of Gene Regulation and Development, Eunice K, Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    BMC Genomics 12:68. 2011
    ....
  56. ncbi request reprint Serine 577 is phosphorylated and negatively affects the tRNA binding and eIF2alpha kinase activities of GCN2
    Minerva Garcia-Barrio
    Laboratory of Gene Regulation and Development, NICHD National Institutes of Health, Building 6A, Rm B1A13, Bethesda, MD 20892, USA
    J Biol Chem 277:30675-83. 2002
    ....
  57. pmc Enhanced interaction between pseudokinase and kinase domains in Gcn2 stimulates eIF2α phosphorylation in starved cells
    Sébastien Lageix
    Laboratory of Gene Regulation and Development, Eunice K Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Genet 10:e1004326. 2014
    ..We propose that tRNA binding to the HisRS domain evokes a conformational change that increases access of the YKD to sites of allosteric activation in the adjoining KD. ..
  58. pmc Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation in vivo
    LEOS VALASEK
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    EMBO J 21:5886-98. 2002
    ....
  59. pmc Active destruction of defective ribosomes by a ubiquitin ligase involved in DNA repair
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 23:891-5. 2009
    ..It now seems likely that the same ubiquitin ligase is enlisted to deal with defective rRNA and damaged DNA...
  60. pmc Mutations that bypass tRNA binding activate the intrinsically defective kinase domain in GCN2
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Genes Dev 16:1271-80. 2002
    ..Our results imply a two-step activation mechanism involving distinct conformational changes in the PK domain...
  61. pmc Gcn4p, a master regulator of gene expression, is controlled at multiple levels by diverse signals of starvation and stress
    Alan G Hinnebusch
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Eukaryot Cell 1:22-32. 2002
  62. ncbi request reprint Functional interactions between yeast translation eukaryotic elongation factor (eEF) 1A and eEF3
    Monika Anand
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 278:6985-91. 2003
    ..In addition, this mutant form of eEF1A shows reduced binding of eEF3. Thus, optimal in vivo interactions between eEF3 and eEF1A are critical for protein synthesis...
  63. pmc The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection
    Hui He
    Division of Biology, Kansas State University, Manhattan, KS 66506, USA
    Mol Cell Biol 23:5431-45. 2003
    ..These results strongly suggest that the binding of the eIF4G HEAT domain to eIF1 and eIF5 is important for maintaining the integrity of the scanning ribosomal preinitiation complex...
  64. pmc Interplay between GCN2 and GCN4 expression, translation elongation factor 1 mutations and translational fidelity in yeast
    Tanya Magazinnik
    Department of Molecular Genetics, Microbiology and Immunology, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Nucleic Acids Res 33:4584-92. 2005
    ..Taken together, this system improves in vivo strategies for the analysis of translational fidelity and further provides new information on the interplay among translation fidelity, altered elongation and translational control via uORFs...
  65. pmc eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA
    Bela Szamecz
    Laboratory of Regulation of Gene Expression, Institute of Microbiology AVCR, Prague, Videnska 1083, 142 20, The Czech Republic
    Genes Dev 22:2414-25. 2008
    ....
  66. pmc Ribosomal protein L33 is required for ribosome biogenesis, subunit joining, and repression of GCN4 translation
    Pilar Martin-Marcos
    Instituto de Microbiologia Bioquimica, CSIC Universidad de Salamanca, Edificio Departamental de Biología, Campus Miguel de Unamuno, 37007 Salamanca, Spain
    Mol Cell Biol 27:5968-85. 2007
    ..We further demonstrate that rpl33a-G76R impairs the efficient processing of 35S and 27S pre-rRNAs and reduces the accumulation of all four mature rRNAs, indicating an important role for L33 in the biogenesis of both ribosomal subunits...
  67. pmc Development and characterization of a reconstituted yeast translation initiation system
    Mikkel A Algire
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    RNA 8:382-97. 2002
    ....
  68. ncbi request reprint IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation
    Catia M Pereira
    Departamentos de Microbiologia, Imunologia, e Parasitologia and Fisiologia, Universidade Federal de Sao Paulo, São Paulo SP 04023 062, Brazil
    J Biol Chem 280:28316-23. 2005
    ..These results suggest that IMPACT ensures constant high levels of translation and low levels of ATF4 and CHOP in specific neuronal cells under amino acid starvation conditions...
  69. ncbi request reprint Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2
    Anil K Padyana
    Structural GenomiX, Inc, San Diego, CA 92121, USA
    J Biol Chem 280:29289-99. 2005
    ..We propose that intramolecular interactions following tRNA binding remodel the hinge region in a manner similar to the mechanism of enzyme activation elicited by the R794G mutation...
  70. pmc Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae
    Sujatha Kadaba
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53201, USA
    Genes Dev 18:1227-40. 2004
    ..These data indicate that a tRNA surveillance pathway exists in yeast that requires Trf4p and the exosome for polyadenylation and degradation of hypomodified pre-tRNA(i)(Met)...
  71. ncbi request reprint Analysis and reconstitution of translation initiation in vitro
    Katsura Asano
    Department of Biology, Kansas State University, Manhattan, Kansas 66506, USA
    Methods Enzymol 351:221-47. 2002
  72. ncbi request reprint New modes of translational control in development, behavior, and disease
    Nahum Sonenberg
    Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada
    Mol Cell 28:721-9. 2007
    ....
  73. ncbi request reprint Targeting translation in hypoxic tumors
    David Ron
    Skirball Institute of Biomolecular Medicine and the Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    ACS Chem Biol 1:145-8. 2006
    ....
  74. ncbi request reprint In vivo stabilization of preinitiation complexes by formaldehyde cross-linking
    LEOS VALASEK
    Institute of Microbiology, AS CR, Prague, Czech Republic
    Methods Enzymol 429:163-83. 2007
    ..In addition, different conditions for displaying the polysome profile or PIC analysis by SDC, used to address different questions, will be outlined...
  75. ncbi request reprint eIF2alpha kinases provide a new solution to the puzzle of substrate specificity
    Alan G Hinnebusch
    Nat Struct Mol Biol 12:835-8. 2005
  76. ncbi request reprint A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II
    Henri Marc Bourbon
    Mol Cell 14:553-7. 2004