Research Topics
| Alison Burgess HickmanSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
|
Detail Information
Publications
Transposition of hAT elements links transposable elements and V(D)J recombinationLiqin Zhou
Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Nature 432:995-1001. 2004....
Unexpected structural diversity in DNA recombination: the restriction endonuclease connectionA B Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell 5:1025-34. 2000..This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition...
Structural unity among viral origin binding proteins: crystal structure of the nuclease domain of adeno-associated virus RepAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 10:327-37. 2002..The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily...
Integrating prokaryotes and eukaryotes: DNA transposases in light of structureAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA
Crit Rev Biochem Mol Biol 45:50-69. 2010..Detailed understanding of transposition pathways is a prerequisite for the long-term goal of exploiting DNA transposons as genetic tools and as a basis for genetic medical applications...
Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selectionOrsolya Barabas
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell 132:208-20. 2008..Our structural results explain why the transposon ends are asymmetric and how the transposon selects a target site for integration, and they allow us to propose a molecular model for the entire transposition reaction...
The carboxy-terminal portion of TnsC activates the Tn7 transposase through a specific interaction with TnsADonald R Ronning
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
EMBO J 23:2972-81. 2004....
The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfacesAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 USA
Mol Cell 13:403-14. 2004..Use of these different interfaces generates the structural asymmetry necessary to regulate later events in viral replication and integration...
DNA recognition and the precleavage state during single-stranded DNA transposition in D. radioduransAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
EMBO J 29:3840-52. 2010..Finally, the observed active site rearrangements when the transposase binds a metal ion in which it is inactive provide a clear rationale for metal ion specificity...
A mob of repsFred Dyda
Laboratory of Molecular Biology, HHS/NIH/NIDDK, Building 5, Room 303, Bethesda, MD 20892, USA
Structure 11:1310-1. 2003..The two classes are united by the same endonucleolytic mechanism and a conserved Mg(2+) binding site containing multiple histidine ligands unique to this superfamily...
The amino acid linker between the endonuclease and helicase domains of adeno-associated virus type 5 Rep plays a critical role in DNA-dependent oligomerizationJenna E Maggin
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
J Virol 86:3337-46. 2012..We conclude that the linker is likely a key contributor to Rep68/78 DNA-dependent oligomerization and may play an important role in mediating Rep68/78's conversion from site-specific DNA binding to nonspecific DNA unwinding...
Binding and unwinding: SF3 viral helicasesAlison Burgess Hickman
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 5 Center Drive MSC 0560, Bethesda, MD 20892-0560, USA
Curr Opin Struct Biol 15:77-85. 2005..At these forks, the SF3 helicases act as replicative helicases. The simian virus 40 SF3 helicase forms a hexameric ring, anticipated to be characteristic of the entire superfamily...
Structural basis for dimerization of LAP2alpha, a component of the nuclear laminaChristina Marchetti Bradley
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Structure 15:643-53. 2007..The surface involved in binding lamin A/C is proposed based on results from alanine-scanning mutagenesis and a solid-phase overlay binding assay...
Purification, crystallization and preliminary crystallographic analysis of the Hermes transposaseZhanita N Perez
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA
Acta Crystallograph Sect F Struct Biol Cryst Commun 61:587-90. 2005..An N-terminally deleted version of the Hermes transposase (residues 79-612) has been overexpressed and purified, and crystals that diffract to 2.1 A resolution have been obtained at 277 K by the hanging-drop method...
Two 14-3-3 binding motifs are required for stable association of Forkhead transcription factor FOXO4 with 14-3-3 proteins and inhibition of DNA bindingTomas Obsil
Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, 5 Center Drive, MSC 0560, Bethesda, Maryland 20892, USA
Biochemistry 42:15264-72. 2003....
The emerging diversity of transpososome architecturesFred Dyda
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Q Rev Biophys 45:493-521. 2012..Given that there are a number of common functional requirements for transposition, it is remarkable how these are satisfied by complex assemblies that are so architecturally different...
In vitro reconstitution of a single-stranded transposition mechanism of IS608Catherine Guynet
Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
Mol Cell 29:302-12. 2008..This single-strand transposition mode has important implications not only for dispersion of IS608 but also for the other members of this very large IS family...
