P N Hengen

Summary

Affiliation: National Cancer Institute
Country: USA

Publications

  1. ncbi request reprint False positives from the yeast two-hybrid system
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:33-4. 1997
  2. ncbi request reprint Optimizing multiplex and LA-PCR with betaine
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:225-6. 1997
  3. ncbi request reprint Methods and reagents. Degraded DNA and gel tornados
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:138-9. 1997
  4. ncbi request reprint Methods and reagents. Reducing background colonies with positive selection vectors
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:105-6. 1997
  5. ncbi request reprint Methods and reagents. Emergency sterilization using microwaves
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:68-9. 1997
  6. ncbi request reprint Methods and reagents. Bionet.molbio.methds-reagnts--a status report
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:359-60. 1997
  7. ncbi request reprint Methods and reagents. Eliminating ghost bands from plasmid preps
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center 21702 1201, USA
    Trends Biochem Sci 21:441-2. 1996
  8. ncbi request reprint Methods and reagents. Purification of GST fusion proteins
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:400-1. 1996
  9. ncbi request reprint Methods and reagents. Eliminating banding artifacts from SDS-PAGE
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 21:191-3. 1996
  10. ncbi request reprint Methods and reagents. preparing ultra-competent Escherichia coli
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:75-6. 1996

Collaborators

Detail Information

Publications18

  1. ncbi request reprint False positives from the yeast two-hybrid system
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:33-4. 1997
    ..This month's column discusses false positives found when using the yeast two-hybrid system to isolate interactive proteins. For details on how to partake in the newsgroup, see the accompanying box...
  2. ncbi request reprint Optimizing multiplex and LA-PCR with betaine
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:225-6. 1997
    ..For details on how to partake in the newsgroup, see the accompanying box...
  3. ncbi request reprint Methods and reagents. Degraded DNA and gel tornados
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:138-9. 1997
    ..molbio.methds-reagnts, available on the Internet. This month's column discusses a case of inexplicable DNA degradation and tornados seen in agarose gels. For details on how to partake in the newsgroup, see the accompanying box...
  4. ncbi request reprint Methods and reagents. Reducing background colonies with positive selection vectors
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:105-6. 1997
    ..This month's column discusses the pros and cons of eliminating unwanted background colonies by using the positive selection vector pZErO. For details on how to partake in the newsgroup, see the accompanying box...
  5. ncbi request reprint Methods and reagents. Emergency sterilization using microwaves
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:68-9. 1997
    ..molbio.methds-reagnts, available on the internet. This month's column discusses the use of a home microwave oven for sterilizing laboratory equipment. For details on how to partake in the newsgroup, see the accompanying box...
  6. ncbi request reprint Methods and reagents. Bionet.molbio.methds-reagnts--a status report
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 22:359-60. 1997
    ..For details on how to join the discussions on bionet, see the accompanying box...
  7. ncbi request reprint Methods and reagents. Eliminating ghost bands from plasmid preps
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center 21702 1201, USA
    Trends Biochem Sci 21:441-2. 1996
    ..molbio.methds-reagnts, available on the internet. This month's column updates the discussion of a ghost DNA band that continues to haunt netters. For details on how to partake in the newsgroup, see the accompanying box...
  8. ncbi request reprint Methods and reagents. Purification of GST fusion proteins
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:400-1. 1996
    ..For details on how to partake in the newsgroup, see the accompanying box...
  9. ncbi request reprint Methods and reagents. Eliminating banding artifacts from SDS-PAGE
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 21:191-3. 1996
    ..For details on how to partake in the newsgroup, see the accompanying box...
  10. ncbi request reprint Methods and reagents. preparing ultra-competent Escherichia coli
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:75-6. 1996
    ..This month's column discusses how to get the most out of your transformation procedure and how to prepare your own ultra-competent Escherichia coli cells. For details on how to partake in the newsgroup, see the accompanying box...
  11. ncbi request reprint Cycle sequencing through GC-rich regions
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:33-4. 1996
    ..For details on how to partake in the newsgroup, see the accompanying box...
  12. ncbi request reprint Methods and reagents. Is your fluorometer on the blink?
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:317-8. 1996
    ..This month's column discusses some common problems associated with measuring DNA concentrations using a mini-fluorometer and the handling of RNA samples. For details on how to partake in the newsgroup, see the accompanying box...
  13. ncbi request reprint Methods and reagents. Pouring sequencing gels the old-fashioned way
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:273-4. 1996
    ..This month's column describes some different ways for pouring DNA sequencing gels, and there are some other tips as well. For details on how to partake in the newsgroup, see the accompanying box...
  14. ncbi request reprint Carriers for precipitating nucleic acids
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702 1201, USA
    Trends Biochem Sci 21:224-5. 1996
    ..molbio.methds-reagnts, available on the Internet. This month's column provides some tips for the precipitation of DNA and RNA samples. For details on how to partake in the newsgroup, see the accompanying box...
  15. ncbi request reprint Shearing DNA for genomic library construction
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 22:273-4. 1997
    ..molibio.methds-reagnts, available on the internet. This month's column discusses the pros and cons of various techniques used to shear DNA for shotgun cloning. For details on how to partake in the newsgroup, see the accompanying box...
  16. pmc The P1 phage replication protein RepA contacts an otherwise inaccessible thymine N3 proton by DNA distortion or base flipping
    I G Lyakhov
    Intramural Research Support Program, SAIC, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USA
    Nucleic Acids Res 29:4892-900. 2001
    ..After protein binding, base pair distortion or base flipping could initiate DNA melting as the second step in DNA replication...
  17. pmc Information analysis of Fis binding sites
    P N Hengen
    Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, PO Box B, Building 469, Room 144, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 25:4994-5002. 1997
    ..A sequence in aldB previously reported to be a Fis site, for which information theory predicts no site, did not shift. These results demonstrate that information analysis is useful for predicting Fis DNA binding...
  18. ncbi request reprint Methods and reagents. Is there any sense in antisense terminology?
    P N Hengen
    National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702 1201, USA
    Trends Biochem Sci 21:153-4. 1996
    ..methds-reagnts, available on the internet. This month's column reviews a recent debate held over the present sense and antisense terminology when referring to DNA. For details on how to partake in the newsgroup, see the accompanying box...