Robert A Heinzen

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    MBio 2:e00175-11. 2011
  2. pmc Bacterial colonization of host cells in the absence of cholesterol
    Stacey D Gilk
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America
    PLoS Pathog 9:e1003107. 2013
  3. pmc Advances in genetic manipulation of obligate intracellular bacterial pathogens
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health Hamilton, MT, USA
    Front Microbiol 2:97. 2011
  4. pmc Sec-mediated secretion by Coxiella burnetii
    Christopher M Stead
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    BMC Microbiol 13:222. 2013
  5. doi request reprint Life on the outside: the rescue of Coxiella burnetii from its host cell
    Anders Omsland
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Annu Rev Microbiol 65:111-28. 2011
  6. ncbi request reprint Rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators
    Robert A Heinzen
    Department of Molecular Biology, University of Wyoming, Laramie 82071 3944, USA
    Ann N Y Acad Sci 990:535-47. 2003
  7. pmc Temporal analysis of Coxiella burnetii morphological differentiation
    Sherry A Coleman
    Coxiella Pathogenesis Unit, NIAID NIH, Rocky Mountain Laboratories, 903 S 4th Street, Hamilton, MT 59840, USA
    J Bacteriol 186:7344-52. 2004
  8. pmc Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S 4th Street, Hamilton, MT 59840, USA
    Infect Immun 77:642-56. 2009
  9. pmc Host cell-free growth of the Q fever bacterium Coxiella burnetii
    Anders Omsland
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 106:4430-4. 2009
  10. pmc Proteome and antigen profiling of Coxiella burnetii developmental forms
    Sherry A Coleman
    Coxiella Pathogenesis Section, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 75:290-8. 2007

Collaborators

Detail Information

Publications52

  1. pmc Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    MBio 2:e00175-11. 2011
    ....
  2. pmc Bacterial colonization of host cells in the absence of cholesterol
    Stacey D Gilk
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America
    PLoS Pathog 9:e1003107. 2013
    ..These data demonstrate that cholesterol is not essential for invasion and intracellular replication by S. Typhimurium and C. trachomatis, but plays a role in C. burnetii-host cell interactions...
  3. pmc Advances in genetic manipulation of obligate intracellular bacterial pathogens
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health Hamilton, MT, USA
    Front Microbiol 2:97. 2011
    ..In this review, we discuss the technical challenges associated with genetic transformation of obligate intracellular bacteria and highlight advances made with individual genera...
  4. pmc Sec-mediated secretion by Coxiella burnetii
    Christopher M Stead
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    BMC Microbiol 13:222. 2013
    ..However, additional secretion systems are likely responsible for modification of the PV lumen microenvironment that promote pathogen replication...
  5. doi request reprint Life on the outside: the rescue of Coxiella burnetii from its host cell
    Anders Omsland
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Annu Rev Microbiol 65:111-28. 2011
    ..This advance removes the extensive experimental obstacles associated with intracellular obligatism and opens the door for a renaissance in C. burnetii research...
  6. ncbi request reprint Rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators
    Robert A Heinzen
    Department of Molecular Biology, University of Wyoming, Laramie 82071 3944, USA
    Ann N Y Acad Sci 990:535-47. 2003
    ..flexneri actin tails but not within R. rickettsii actin tails. Collectively, these data suggest that rickettsial ABM is independent of N-WASP and Arp2/3 complex function...
  7. pmc Temporal analysis of Coxiella burnetii morphological differentiation
    Sherry A Coleman
    Coxiella Pathogenesis Unit, NIAID NIH, Rocky Mountain Laboratories, 903 S 4th Street, Hamilton, MT 59840, USA
    J Bacteriol 186:7344-52. 2004
    ..The experimental model described in this report will allow a global transcriptome and proteome analysis of C. burnetii developmental forms...
  8. pmc Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S 4th Street, Hamilton, MT 59840, USA
    Infect Immun 77:642-56. 2009
    ..The observation that the attenuated Dugway isolate has the largest genome with the fewest pseudogenes and IS elements suggests that this isolate's lineage is at an earlier stage of pathoadaptation than the NM, K, and G lineages...
  9. pmc Host cell-free growth of the Q fever bacterium Coxiella burnetii
    Anders Omsland
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 106:4430-4. 2009
    ..Furthermore, the systematic approach used here may be broadly applicable to development of axenic media that support growth of other medically important obligate intracellular pathogens...
  10. pmc Proteome and antigen profiling of Coxiella burnetii developmental forms
    Sherry A Coleman
    Coxiella Pathogenesis Section, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 75:290-8. 2007
    ..burnetii and novel immunogenic proteins that may be important in the pathophysiology of clinical Q fever and/or the induction of protective immunity...
  11. pmc Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesis
    Paul A Beare
    National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 191:1369-81. 2009
    ..The mutant FtsZ protein was also unable to bind the essential cell division protein FtsA. This is the first description of C. burnetii harboring a defined gene mutation generated by genetic transformation...
  12. pmc Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray
    Paul A Beare
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, 903 S 4th St, Hamilton, MT 59840, USA
    Clin Vaccine Immunol 15:1771-9. 2008
    ..This sensitive and high-throughput method for identifying immunoreactive C. burnetii proteins will aid in the development of Q fever serodiagnostic tests based on recombinant antigen...
  13. pmc The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion
    Daniel E Voth
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 191:4232-42. 2009
    ..Collectively, these data suggest that C. burnetii isolates translocate distinct subsets of the Ank protein family into the host cytosol, where they modulate diverse functions, some of which may be unique to C. burnetii pathotypes...
  14. pmc Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella pneumophila revealed by coinfection
    John Demian Sauer
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S 4th St, Hamilton, MT 59840, USA
    Infect Immun 73:4494-504. 2005
    ..pneumophila replication vacuole. Moreover, L. pneumophila can replicate efficiently in either lysosomal vacuoles of A/J mouse cells or in nonlysosomal vacuoles of primate cells...
  15. pmc Isolation from animal tissue and genetic transformation of Coxiella burnetii are facilitated by an improved axenic growth medium
    Anders Omsland
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Appl Environ Microbiol 77:3720-5. 2011
    ..Cultivation in ACCM-2 will accelerate development of C. burnetii genetic tools and provide a sensitive means of primary isolation of the pathogen from Q fever patients...
  16. ncbi request reprint Fusogenicity of the Coxiella burnetii parasitophorous vacuole
    Dale Howe
    University of Wyoming, Department of Molecular Biology, Laramie, Wyoming 82071 3944, USA
    Ann N Y Acad Sci 990:556-62. 2003
    ..These results suggest that sustained C. burnetii protein synthesis is required for PV fusion with other vacuoles of the endocytic pathway...
  17. pmc Efficient method of cloning the obligate intracellular bacterium Coxiella burnetii
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, 903 S 4th St, Hamilton, MT 59840, USA
    Appl Environ Microbiol 73:4048-54. 2007
    ..Without exception, PVs obtained from second-round excisions contained clonal populations of either NMII or NMC, demonstrating that micromanipulation is an efficient and reproducible procedure for obtaining C. burnetii clones...
  18. pmc Genetic diversity of the Q fever agent, Coxiella burnetii, assessed by microarray-based whole-genome comparisons
    Paul A Beare
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    J Bacteriol 188:2309-24. 2006
    ..Our results provide new insight into the genetic diversity and virulence potential of Coxiella species...
  19. ncbi request reprint Essential role for the response regulator PmrA in Coxiella burnetii type 4B secretion and colonization of mammalian host cells
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Bacteriol 196:1925-40. 2014
    ..burnetii proteins involved in host cell parasitism. ..
  20. ncbi request reprint Infection of human monocyte-derived macrophages with Coxiella burnetii
    Jeffrey G Shannon
    Coxiella Pathogenesis Section, Laboratory of Interacellular Parasites, Rocky Mountain Laboratoties, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA
    Methods Mol Biol 431:189-200. 2008
    ....
  21. pmc The Chlamydia trachomatis plasmid is a transcriptional regulator of chromosomal genes and a virulence factor
    John H Carlson
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Infect Immun 76:2273-83. 2008
    ..Our findings have important implications in understanding a role for the plasmid in the pathogenesis of human infection and disease...
  22. pmc Coxiella burnetii effector protein subverts clathrin-mediated vesicular trafficking for pathogen vacuole biogenesis
    Charles L Larson
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840
    Proc Natl Acad Sci U S A 110:E4770-9. 2013
    ..burnetii replication. Thus, our results reveal the importance of clathrin-coated vesicle trafficking in C. burnetii infection and define a role for CvpA in subverting these transport mechanisms. ..
  23. pmc Fractionation of the Coxiella burnetii parasitophorous vacuole
    Dale Howe
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA
    Methods Mol Biol 445:389-406. 2008
    ..burnetii PV and LBP. Identification of proteins unique to the PV membrane will yield important insight into C. burnetii-host interactions...
  24. ncbi request reprint Maturation of the Coxiella burnetii parasitophorous vacuole requires bacterial protein synthesis but not replication
    Dale Howe
    Department of Molecular Biology, University of Wyoming, Laramie, Wyoming, 82071 3944, USA
    Cell Microbiol 5:469-80. 2003
    ..However, transit of nascent PV through the endocytic pathway to ultimately acquire lysosomal markers appears to occur irrespective of Coxiella protein synthesis...
  25. pmc Antibody-mediated immunity to the obligate intracellular bacterial pathogen Coxiella burnetii is Fc receptor- and complement-independent
    Jeffrey G Shannon
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    BMC Immunol 10:26. 2009
    ..The mechanism of this Ab-mediated protection is unknown. Therefore, we conducted a study to determine whether Fc receptors (FcR) or complement contribute to Ab-mediated immunity (AMI) to C. burnetii...
  26. pmc Adaptive immunity to the obligate intracellular pathogen Coxiella burnetii
    Jeffrey G Shannon
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, 903 S 4th Street, Hamilton, MT 59840, USA
    Immunol Res 43:138-48. 2009
    ..burnetii are needed. This review summarizes our current understanding of adaptive immunity to C. burnetii with a focus on recent developments in the field...
  27. pmc A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells
    Diane C Cockrell
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    J Microbiol Methods 72:321-5. 2008
    ..burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication...
  28. pmc Sustained axenic metabolic activity by the obligate intracellular bacterium Coxiella burnetii
    Anders Omsland
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 190:3203-12. 2008
    ..burnetii in CCM provides an important tool to investigate the physiology of this organism including developmental transitions and responses to antimicrobial factors associated with the host cell...
  29. ncbi request reprint Coxiella burnetii inhabits a cholesterol-rich vacuole and influences cellular cholesterol metabolism
    Dale Howe
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Cell Microbiol 8:496-507. 2006
    ..We conclude that C. burnetii infection perturbs host cell cholesterol metabolism and that free access to host cholesterol stores is required for optimal C. burnetii replication...
  30. pmc Coxiella type IV secretion and cellular microbiology
    Daniel E Voth
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Curr Opin Microbiol 12:74-80. 2009
    ..Functional characterization of the biological activity of these effector proteins will dramatically aid our ability to model Coxiella-host cell interactions...
  31. pmc Coxiella burnetii expresses a functional Δ24 sterol reductase
    Stacey D Gilk
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 192:6154-9. 2010
    ..These data indicate CBU1206 is an active sterol reductase and suggest the enzyme may act on host sterols during C. burnetii intracellular growth...
  32. pmc Virulent Coxiella burnetii does not activate human dendritic cells: role of lipopolysaccharide as a shielding molecule
    Jeffrey G Shannon
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 102:8722-7. 2005
    ..This immune evasion strategy may allow C. burnetii to persist in an immunocompetent host...
  33. ncbi request reprint Replication of Coxiella burnetii is inhibited in CHO K-1 cells treated with inhibitors of cholesterol metabolism
    Dale Howe
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    Ann N Y Acad Sci 1063:123-9. 2005
    ..These data suggest that cholesterol is an important component of the C. burnetii PV membrane and that normal cellular cholesterol metabolism is required for optimal C. burnetii replication...
  34. pmc Coxiella burnetii phase I and II variants replicate with similar kinetics in degradative phagolysosome-like compartments of human macrophages
    Dale Howe
    Coxiella Pathogenesis Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 78:3465-74. 2010
    ..burnetii does not actively inhibit phagolysosome function as a survival mechanism. Instead, NMI and NMII resist degradation to replicate in indistinguishable digestive PVs that fully mature through the endolysosomal pathway...
  35. ncbi request reprint Lack of dendritic cell maturation following infection by Coxiella burnetii synthesizing different lipopolysaccharide chemotypes
    Jeffrey G Shannon
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Ann N Y Acad Sci 1063:154-60. 2005
    ..Our data indicate that LPS chemotype is not a determinant of DC maturation or cytokine production in response to C. burnetii...
  36. pmc Two systems for targeted gene deletion in Coxiella burnetii
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    Appl Environ Microbiol 78:4580-9. 2012
    ..dotA and ΔdotB mutants failed to secrete T4BSS substrates and to productively infect host cells. The repertoire of C. burnetii genetic tools now allows ready fulfillment of molecular Koch's postulates for suspected virulence genes...
  37. pmc Coxiella burnetii inhibits apoptosis in human THP-1 cells and monkey primary alveolar macrophages
    Daniel E Voth
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, 903 S 4th Street, Hamilton, MT 59840, USA
    Infect Immun 75:4263-71. 2007
    ..Collectively, these data suggest that C. burnetii modulates apoptotic pathways to inhibit host cell death, thus providing a stable, intracellular niche for the course of the pathogen's infectious cycle...
  38. ncbi request reprint Comparative DNA microarray analysis of host cell transcriptional responses to infection by Coxiella burnetii or Chlamydia trachomatis
    Qun Ren
    Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 3944, USA
    Ann N Y Acad Sci 990:701-13. 2003
    ..burnetii. This study provides insight into the host transcriptional responses to infection by Chlamydia and Coxiella that may affect the infectious cycle of each organism...
  39. pmc Nitric oxide inhibits Coxiella burnetii replication and parasitophorous vacuole maturation
    Dale Howe
    Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 3944, USA
    Infect Immun 70:5140-7. 2002
    ..burnetii cells. These experiments demonstrate that nitric oxide reversibly inhibits replication of C. burnetii and formation of the parasitophorous vacuole...
  40. ncbi request reprint Preliminary assessment of genome differences between the reference Nine Mile isolate and two human endocarditis isolates of Coxiella burnetii
    Paul A Beare
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA
    Ann N Y Acad Sci 1063:64-7. 2005
    ..burnetii and to provide important insight into the genome architecture and genetic diversity of this pathogen...
  41. pmc Host-microbe interaction systems biology: lifecycle transcriptomics and comparative genomics
    Daniel E Sturdevant
    Genomics Unit, Research Technologies Section, Research Technologies Branch, Rocky Mountain Laboratories, NIH, 904 South 4th Street, Hamilton, MT 59840, USA
    Future Microbiol 5:205-19. 2010
    ..Together, microarray and comparative genomic technologies will continue to advance our understanding of pathogen evolution and assist in combating human infectious disease...
  42. ncbi request reprint Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii
    Daniel E Voth
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Cell Microbiol 9:829-40. 2007
    ..Here we summarize our current understanding of the cellular events that occur during parasitism of host cells by Coxiella...
  43. pmc Sustained activation of Akt and Erk1/2 is required for Coxiella burnetii antiapoptotic activity
    Daniel E Voth
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, 903 S 4th Street, Hamilton, MT 59840, USA
    Infect Immun 77:205-13. 2009
    ..Collectively, these results indicate the importance of C. burnetii modulation of host signaling and demonstrate a critical role for Akt and Erk1/2 in successful intracellular parasitism and maintenance of host cell viability...
  44. ncbi request reprint Developmental transitions of Coxiella burnetii grown in axenic media
    Kelsi M Sandoz
    Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, United States
    J Microbiol Methods 96:104-10. 2014
    ..Axenic cultivation of C. burnetii in ACCM-2 and new methods of genetic manipulation now allow investigation of the molecular basis of C. burnetii biphasic development. ..
  45. pmc Effects of ectopically expressed neuronal Wiskott-Aldrich syndrome protein domains on Rickettsia rickettsii actin-based motility
    Ronald S Harlander
    Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071 3944, USA
    Infect Immun 71:1551-6. 2003
    ..These results, taken together with the differential effects on motility observed upon expression of other N-WASP domains, suggest that actin-based motility of R. rickettsii is independent of N-WASP and the Arp2/3 complex...
  46. pmc Functional BvgAS virulence control system in Bordetella bronchiseptica is necessary for induction of Ca2+ transients in ciliated tracheal epithelial cells
    Nathan A Groathouse
    Department of Zoology and Physiology, University of Wyoming, Laramie, Wyoming 82701, USA
    Infect Immun 71:7208-10. 2003
    ..The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense...
  47. ncbi request reprint Identification of two putative rickettsial adhesins by proteomic analysis
    Patricia Renesto
    Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
    Res Microbiol 157:605-12. 2006
    ..Both proteins are highly conserved within the Rickettsia genus and might play a critical role in their pathogenicity. These data may have important implications for the development of future vaccines against rickettsial infections...
  48. ncbi request reprint A Rickettsia WASP-like protein activates the Arp2/3 complex and mediates actin-based motility
    Robert L Jeng
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell Microbiol 6:761-9. 2004
    ..We propose that additional Rickettsia or host factors reorganize the Y-branched networks into parallel arrays in a manner similar to a recently proposed model of filopodia formation...
  49. doi request reprint High-throughput identification of T-lymphocyte antigens from Anaplasma marginale expressed using in vitro transcription and translation
    Job E Lopez
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, United States
    J Immunol Methods 332:129-41. 2008
    ..This method allows rapid expression and identification of T-lymphocyte antigens for any pathogen for which the genome sequence is available...
  50. pmc Complete genome sequence of the Q-fever pathogen Coxiella burnetii
    Rekha Seshadri
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 100:5455-60. 2003
    ..burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation...
  51. ncbi request reprint Identification of Coxiella burnetii RpoS-dependent promoters
    Jana Melnicáková
    Institute of Virology, Slovak Academy of Sciences, 842 45 Bratislava, Slovak Republic
    Ann N Y Acad Sci 990:591-5. 2003