Robert A Heinzen
Affiliation: National Institutes of Health
Moormeier D, Sandoz K, Beare P, Sturdevant D, Nair V, Cockrell D, et al
. Coxiella burnetii RpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth. J Bacteriol. 2019;201: pubmed publisher
..These results support RpoS as a central regulator of the Coxiella developmental cycle and identify developmentally regulated genes involved in morphological differentiation. ..
Larson C, Sandoz K, Cockrell D, Heinzen R. Noncanonical Inhibition of mTORC1 by Coxiella burnetii Promotes Replication within a Phagolysosome-Like Vacuole. MBio. 2019;10: pubmed publisher
..burnetii growth. Furthermore, hyperactivation of mTORC1 impairs bacterial replication. These findings indicate that C. burnetii inhibition of mTORC1 without accelerated autophagy promotes bacterial growth. ..
Sandoz K, Sturdevant D, Hansen B, Heinzen R. Developmental transitions of Coxiella burnetii grown in axenic media. J Microbiol Methods. 2014;96:104-10 pubmed publisher
..Axenic cultivation of C. burnetii in ACCM-2 and new methods of genetic manipulation now allow investigation of the molecular basis of C. burnetii biphasic development. ..
Larson C, Beare P, Voth D, Howe D, Cockrell D, Bastidas R, et al
. Coxiella burnetii effector proteins that localize to the parasitophorous vacuole membrane promote intracellular replication. Infect Immun. 2015;83:661-70 pubmed publisher
..Collectively, these data indicate C. burnetii encodes multiple effector proteins that target the PV membrane and benefit pathogen replication in human macrophages. ..
Stead C, Cockrell D, Beare P, Miller H, Heinzen R. A Coxiella burnetii phospholipase A homolog pldA is required for optimal growth in macrophages and developmental form lipid remodeling. BMC Microbiol. 2018;18:33 pubmed publisher
..burnetii contributes to colonization of macrophages and may contribute to the environmental stability and the distinct biological properties of the SCV. ..
Sandoz K, Beare P, Cockrell D, Heinzen R. Complementation of Arginine Auxotrophy for Genetic Transformation of Coxiella burnetii by Use of a Defined Axenic Medium. Appl Environ Microbiol. 2016;82:3042-51 pubmed publisher
..burnetii metabolism and allowing non-antibiotic-based selection of C. burnetii genetic transformants, an important achievement considering that selectable makers based on antibiotic resistance are limited. ..
Heinzen R. Rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators. Ann N Y Acad Sci. 2003;990:535-47 pubmed
..flexneri actin tails but not within R. rickettsii actin tails. Collectively, these data suggest that rickettsial ABM is independent of N-WASP and Arp2/3 complex function. ..
Omsland A, Heinzen R. Life on the outside: the rescue of Coxiella burnetii from its host cell. Annu Rev Microbiol. 2011;65:111-28 pubmed publisher
..This advance removes the extensive experimental obstacles associated with intracellular obligatism and opens the door for a renaissance in C. burnetii research...
Beare P, Jeffrey B, Martens C, Heinzen R. Draft Genome Sequences of Three Coxiella burnetii Strains Isolated from Q Fever Patients. Genome Announc. 2017;5: pubmed publisher
..The Coxiella burnetii Turkey (RSA315) and Dyer (RSA345) strains were isolated from acute Q fever patients, while the Ko (Q229) strain was isolated from a Q fever endocarditis patient. ..