V J Hearing

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation
    Taketsugu Tadokoro
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 124:1326-32. 2005
  2. ncbi request reprint Regulating melanosome transfer: who's driving the bus?
    Vincent J Hearing
    Pigment Cell Res 20:334-5. 2007
  3. pmc The Roles of ADAMs Family Proteinases in Skin Diseases
    Masakazu Kawaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA
    Enzyme Res 2011:482498. 2011
  4. pmc Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes
    Masakazu Kawaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 132:2791-9. 2012
  5. ncbi request reprint UV-induced DNA damage and melanin content in human skin differing in racial/ethnic origin
    Taketsugu Tadokoro
    Laboratory of Cell Biology, Building 37, Room 1B25, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    FASEB J 17:1177-9. 2003
  6. ncbi request reprint The melanosome: the perfect model for cellular responses to the environment
    V J Hearing
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Pigment Cell Res 13:23-34. 2000
  7. ncbi request reprint Biogenesis of pigment granules: a sensitive way to regulate melanocyte function
    Vincent J Hearing
    Pigment Cell Biology Section, Laboratory of Cell Biology, Building 37, Room 2132, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Dermatol Sci 37:3-14. 2005
  8. ncbi request reprint Biochemical control of melanogenesis and melanosomal organization
    V J Hearing
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Investig Dermatol Symp Proc 4:24-8. 1999
  9. ncbi request reprint Fatty acids regulate pigmentation via proteasomal degradation of tyrosinase: a new aspect of ubiquitin-proteasome function
    Hideya Ando
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:15427-33. 2004
  10. ncbi request reprint Involvement of ITF2 in the transcriptional regulation of melanogenic genes
    M Furumura
    Pigment Cell Biology Section, Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28147-54. 2001

Collaborators

Detail Information

Publications83

  1. ncbi request reprint Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation
    Taketsugu Tadokoro
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 124:1326-32. 2005
    ..These results provide a basis for understanding the origin of different skin colors and responses to UV within different races...
  2. ncbi request reprint Regulating melanosome transfer: who's driving the bus?
    Vincent J Hearing
    Pigment Cell Res 20:334-5. 2007
  3. pmc The Roles of ADAMs Family Proteinases in Skin Diseases
    Masakazu Kawaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA
    Enzyme Res 2011:482498. 2011
    ..This paper focuses on the roles of ADAMs proteinases in a wide variety of skin diseases...
  4. pmc Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes
    Masakazu Kawaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 132:2791-9. 2012
    ..These results suggest that DGK regulates melanogenesis via modulation of the posttranslational processing of tyrosinase, which may be related with the protein degradation machinery...
  5. ncbi request reprint UV-induced DNA damage and melanin content in human skin differing in racial/ethnic origin
    Taketsugu Tadokoro
    Laboratory of Cell Biology, Building 37, Room 1B25, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    FASEB J 17:1177-9. 2003
    ....
  6. ncbi request reprint The melanosome: the perfect model for cellular responses to the environment
    V J Hearing
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Pigment Cell Res 13:23-34. 2000
    ..Studies targeted at elucidating the regulatory mechanisms involved and the functional changes that result demonstrate that the melanosome is the perfect model to study such biological response mechanisms...
  7. ncbi request reprint Biogenesis of pigment granules: a sensitive way to regulate melanocyte function
    Vincent J Hearing
    Pigment Cell Biology Section, Laboratory of Cell Biology, Building 37, Room 2132, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Dermatol Sci 37:3-14. 2005
    ..Our understanding of the mechanisms behind a wide range of human pigmentary diseases have grown remarkably as melanosomes have been unraveled...
  8. ncbi request reprint Biochemical control of melanogenesis and melanosomal organization
    V J Hearing
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Investig Dermatol Symp Proc 4:24-8. 1999
    ..Because many of those melanosomal proteins also serve as melanoma-specific targets, regulation of their expression has dramatic implications for immune targeting of malignant melanoma...
  9. ncbi request reprint Fatty acids regulate pigmentation via proteasomal degradation of tyrosinase: a new aspect of ubiquitin-proteasome function
    Hideya Ando
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:15427-33. 2004
    ..In broader terms, the function of the ubiquitin-proteasome pathway might be regulated physiologically, at least in part, by fatty acids within cellular membranes...
  10. ncbi request reprint Involvement of ITF2 in the transcriptional regulation of melanogenic genes
    M Furumura
    Pigment Cell Biology Section, Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28147-54. 2001
    ....
  11. ncbi request reprint Production of melanocyte-specific antibodies to human melanosomal proteins: expression patterns in normal human skin and in cutaneous pigmented lesions
    V Virador
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Pigment Cell Res 14:289-97. 2001
    ..Those differences in the expression patterns of melanocyte markers provide important clues to the roles of melanocytes in normal and in disrupted skin pigmentation...
  12. pmc Modulation of murine melanocyte function in vitro by agouti signal protein
    C Sakai
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 16:3544-52. 1997
    ....
  13. pmc Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis
    T Kobayashi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 13:5818-25. 1994
    ..This enzyme activity appears to be essential to the further metabolism of DHICA to a high molecular weight pigmented biopolymer...
  14. pmc A model for melanosome biogenesis based on the purification and analysis of early melanosomes
    T Kushimoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:10698-703. 2001
    ..These results provide a better understanding of the structural features seen during melanosome biogenesis, and they yield further clues as to the physiological regulation of pigmentation...
  15. ncbi request reprint Tyrosinase stabilization by Tyrp1 (the brown locus protein)
    T Kobayashi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda Maryland 20892, USA
    J Biol Chem 273:31801-5. 1998
    ..In sum, these results suggest that, in addition to its catalytic function in oxidizing DHICA, Tyrp1 may play an important role in stabilizing Tyr, a second potential role in the regulation of melanin formation...
  16. pmc Characterization of genes modulated during pheomelanogenesis using differential display
    M Furumura
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 95:7374-8. 1998
    ....
  17. pmc The molecular basis of oculocutaneous albinism type 1 (OCA1): sorting failure and degradation of mutant tyrosinases results in a lack of pigmentation
    K Toyofuku
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 1B25, Bethesda, MD 20892, USA
    Biochem J 355:259-69. 2001
    ....
  18. ncbi request reprint In situ localization of agouti signal protein in murine skin using immunohistochemistry with an ASP-specific antibody
    N Matsunaga
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochem Biophys Res Commun 270:176-82. 2000
    ..These expression patterns suggest that ASP is delivered quickly and efficiently to melanocytes and to hair matrix cells in the hair bulbs where it regulates melanin production...
  19. pmc A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase
    K Tsukamoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 11:519-26. 1992
    ..We now report that TRP-2 (tyrosinase related protein-2), which maps to and is mutated at the slaty locus in mice, encodes a protein with DOPAchrome tautomerase activity...
  20. ncbi request reprint Oculocutaneous albinism types 1 and 3 are ER retention diseases: mutation of tyrosinase or Tyrp1 can affect the processing of both mutant and wild-type proteins
    K Toyofuku
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    FASEB J 15:2149-61. 2001
    ..These results show that OCA1 and OCA3 are (in some cases, at least) ER retention diseases wherein a mutation in one melanogenic protein affects the maturation and stability of the other in the melanogenic pathway...
  21. ncbi request reprint Bioactive motifs of agouti signal protein
    V M Virador
    Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland, 20892, USA
    Exp Cell Res 259:54-63. 2000
    ..Identification of the specific ASP epitope that interacts with the MC1R has potential pharmacological applications in treating dysfunctions of skin pigmentation...
  22. ncbi request reprint Functional analysis of the slaty gene product (TRP2) as dopachrome tautomerase and the effect of a point mutation on its catalytic function
    G Kroumpouzos
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Biochem Biophys Res Commun 202:1060-8. 1994
    ..We further demonstrate that the slaty mutation dramatically decreases the catalytic function of the protein...
  23. ncbi request reprint The Pmel 17/silver locus protein. Characterization and investigation of its melanogenic function
    T Kobayashi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:29198-205. 1994
    ....
  24. ncbi request reprint Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: use in the characterization of the human melanoma antigen MART-1
    Y Kawakami
    Surgery Branch, National Cancer Institute, Bethesda, MD 20892, USA
    J Immunol Methods 202:13-25. 1997
    ..These reagents may be useful for biological studies on melanocytes and melanoma cells as well as for the development and monitoring of immunotherapy for patients with melanoma...
  25. ncbi request reprint Analysis of the transcriptional regulation of melanocytic genes by alphaMSH using the cDNA microarray technique
    F Rouzaud
    Laboratory of Cell Biology, National Institutes of Health, National Cancer Institute, Bethesda, MD 20892, USA
    Cell Mol Biol (Noisy-le-grand) 52:21-31. 2006
    ....
  26. ncbi request reprint Modulation of melanogenic protein expression during the switch from eu- to pheomelanogenesis
    T Kobayashi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 108:2301-9. 1995
    ..Expression of the silver protein was similarly not observed in hair bulbs of the pheomelanic mice.(ABSTRACT TRUNCATED AT 250 WORDS)..
  27. ncbi request reprint Melanosomal tyrosine transport in normal and pink-eyed dilution murine melanocytes
    W A Gahl
    Section on Human Biochemical Genetics, Human Genetics Branch, NICHD, NIH, Bethesda, Maryland 20892 1830, USA
    Pigment Cell Res 8:229-33. 1995
    ..These data indicate that a tyrosine transport system exists in the melanosomal membrane and that the p gene does not encode a tyrosine transporter of critical importance...
  28. ncbi request reprint The nature of tyrosinase isozymes
    K Tsukamoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Pigment Cell Res . 1992
  29. ncbi request reprint Functional properties of cloned melanogenic proteins
    V J Hearing
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Pigment Cell Res 5:264-70. 1992
    ..The results demonstrate the catalytic functions of these proteins and how they stably interact within a melanogenic complex in the melanosome to regulate the quantity and quality of melanin synthesized by the melanocyte...
  30. pmc Different approaches for assaying melanosome transfer
    Werner Berens
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Pigment Cell Res 18:370-81. 2005
    ..This information could give other researchers a head start in the search for a melanosome transfer assay with said qualities to better understand pigment transfer...
  31. pmc Intracellular composition of fatty acid affects the processing and function of tyrosinase through the ubiquitin-proteasome pathway
    Hideya Ando
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH, Building 37, Room 2132, MSC 4256, Bethesda, MD 20892, USA
    Biochem J 394:43-50. 2006
    ....
  32. ncbi request reprint Sorting of Pmel17 to melanosomes through the plasma membrane by AP1 and AP2: evidence for the polarized nature of melanocytes
    Julio C Valencia
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 119:1080-91. 2006
    ..These results show that Pmel17 is sorted to melanosomes by various intracellular routes, directly or indirectly through the plasma membrane, and the presence of basolateral elements in melanocytes suggests their polarized nature...
  33. ncbi request reprint Human skin responses to UV radiation: pigment in the upper epidermis protects against DNA damage in the lower epidermis and facilitates apoptosis
    Yuji Yamaguchi
    Laboratory of Cell Biology, National Institutes of Health, Bldg 37, Rm 2132, Bethesda, Maryland 20892 4254, USA
    FASEB J 20:1486-8. 2006
    ..The combination of decreased DNA damage and more efficient removal of UV-damaged cells may play a critical role in the decreased photocarcinogenesis seen in individuals with darker skin...
  34. ncbi request reprint UV increases the nuclear localization of apurinic/apyrimidinic endonuclease/redox effector factor-1 in human skin
    Kaoruko Takahashi
    J Invest Dermatol 126:2723-6. 2006
  35. ncbi request reprint Photobiologic role of melanin distribution in the epidermis
    Barbara Z Zmudzka
    J Photochem Photobiol B 84:231. 2006
  36. ncbi request reprint Regulation of constitutive and UVR-induced skin pigmentation by melanocortin 1 receptor isoforms
    Francois Rouzaud
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    FASEB J 20:1927-9. 2006
    ....
  37. ncbi request reprint Human skin pigmentation: melanocytes modulate skin color in response to stress
    Gertrude E Costin
    Avon Products, Inc, New Technology Department, 1 Avon Pl, Suffern, NY 10901, USA
    FASEB J 21:976-94. 2007
    ..g., to design tanning products with potential to reduce skin cancer risk)...
  38. ncbi request reprint Regulation of human skin pigmentation and responses to ultraviolet radiation
    Yoshinori Miyamura
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Res 20:2-13. 2007
    ..Further research is needed to characterize the role of skin pigmentation to reduce photocarcinogenesis and to develop effective strategies to minimize such risks...
  39. pmc Regulation of eumelanin/pheomelanin synthesis and visible pigmentation in melanocytes by ligands of the melanocortin 1 receptor
    Elodie Le Pape
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Melanoma Res 21:477-86. 2008
    ....
  40. pmc Pigmentation effects of solar-simulated radiation as compared with UVA and UVB radiation
    Rainer Wolber
    Beiersdorf AG, R and D, Skin Research Center, Hamburg, Germany
    Pigment Cell Melanoma Res 21:487-91. 2008
    ..These results show that SSR is more effective in promoting delayed tanning than UVB radiation alone, suggesting a synergistic effect of UVA radiation. Furthermore, free 5SCD may serve as a good marker of the effect of SSR and UVB...
  41. pmc The protective role of melanin against UV damage in human skin
    Michaela Brenner
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Photochem Photobiol 84:539-49. 2008
    ..This article outlines the major acute and chronic effects of UVR on human skin, the properties of melanin, the regulation of pigmentation and its effect on skin cancer prevention...
  42. ncbi request reprint Direct interaction of tyrosinase with Tyrp1 to form heterodimeric complexes in vivo
    Takeshi Kobayashi
    Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    J Cell Sci 120:4261-8. 2007
    ..Taken together, these data demonstrate that Tyrp1 interacts directly with Tyr in vivo, which may regulate the stability and trafficking of melanogenic enzymes and thus pigment synthesis...
  43. ncbi request reprint Melanin mediated apoptosis of epidermal cells damaged by ultraviolet radiation: factors influencing the incidence of skin cancer
    Yuji Yamaguchi
    Pigment Cell Research Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Arch Dermatol Res 300:S43-50. 2008
    ..The combination of decreased DNA damage and more efficient removal of UV-damaged cells may play a critical role in the decreased photocarcinogenesis seen in individuals with darker skin...
  44. pmc Mesenchymal-epithelial interactions in the skin: increased expression of dickkopf1 by palmoplantar fibroblasts inhibits melanocyte growth and differentiation
    Yuji Yamaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bldg 37, Rm 1B25, Bethesda, MD 20892 4254, USA
    J Cell Biol 165:275-85. 2004
    ....
  45. pmc Involvement of dynein and spectrin with early melanosome transport and melanosomal protein trafficking
    Hidenori Watabe
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 128:162-74. 2008
    ..These results provide important clues toward understanding the processes involved with early events in melanosome formation and transport...
  46. ncbi request reprint The regulation of skin pigmentation
    Yuji Yamaguchi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:27557-61. 2007
  47. pmc SOX9 is a key player in ultraviolet B-induced melanocyte differentiation and pigmentation
    Thierry Passeron
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA
    Proc Natl Acad Sci U S A 104:13984-9. 2007
    ..SOX9 completes the complex and tightly regulated process leading to the production of melanin by acting at a very upstream level. This role of SOX9 in pigmentation emphasizes the poorly understood impact of SOX proteins in adult tissues...
  48. ncbi request reprint Tyrosinase exacerbates dopamine toxicity but is not genetically associated with Parkinson's disease
    Elisa Greggio
    Department of Biology, University of Padova, Padova, Italy
    J Neurochem 93:246-56. 2005
    ..This argues against a strong genetic association between tyrosinase and PD, although the observed contribution to cellular toxicity suggests that a biochemical association is likely...
  49. pmc Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes
    Kevin G Chen
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Res 18:102-12. 2005
    ..Our findings indicate that expression of ABCB 5alpha/beta might possibly provide two novel molecular markers for differential diagnosis of melanomas and constitute potential molecular targets for therapy of melanomas...
  50. ncbi request reprint Down-regulation of melanogenesis by phospholipase D2 through ubiquitin proteasome-mediated degradation of tyrosinase
    Akiko Kageyama
    Division of Dermatology, Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    J Biol Chem 279:27774-80. 2004
    ..This suggests that PLD2 may play an important role in regulating pigmentation in vivo...
  51. ncbi request reprint Co-culture of mouse epidermal cells for studies of pigmentation
    Tae Jin Yoon
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Res 16:159-63. 2003
    ..Thus, we have now optimized co-culture conditions for murine melanocytes and keratinocytes so that pigmentation and the effects of specific mutations can be studied in a more physiologically relevant context...
  52. ncbi request reprint Proteomic analysis of early melanosomes: identification of novel melanosomal proteins
    Venkatesha Basrur
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 2:69-79. 2003
    ....
  53. ncbi request reprint Regulation of tyrosinase processing and trafficking by organellar pH and by proteasome activity
    Hidenori Watabe
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:7971-81. 2004
    ..The sum of these results shows that organellar pH, proteasome activity, and down-regulation of TYRP1 expression all contribute to the lack of pigmentation in TYR-positive amelanotic melanoma cells...
  54. ncbi request reprint The inhibitory effect of androgen and sex-hormone-binding globulin on the intracellular cAMP level and tyrosinase activity of normal human melanocytes
    Taketsugu Tadokoro
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, MD, USA
    Pigment Cell Res 16:190-7. 2003
    ..Our results suggest that androgens may modulate tyrosinase activity at the posttranslational level through the cell membrane signaling pathway...
  55. ncbi request reprint The Seiji memorial lecture: the melanosome: an ideal model to study cellular differentiation
    Tsuneto Kushimoto
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Res 16:237-44. 2003
    ..Recent studies have elucidated the full proteome of the melanosome and the metabolic and molecular lesions involved in a number of pigmentary diseases have been resolved. This paper summarizes recent advances in the field in these areas...
  56. ncbi request reprint Tyrosinase: a developmentally specific major determinant of peripheral dopamine
    Graeme Eisenhofer
    Section on Clinical Neurocardiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 10 Center Dr MSC 1620, Bethesda, MD 20892 1620, USA
    FASEB J 17:1248-55. 2003
    ..The transient nature of this source of dopamine reflects a developmental switch in tyrosinase-dependent production of dopamine to production of melanin...
  57. ncbi request reprint Tyrosinase processing and intracellular trafficking is disrupted in mouse primary melanocytes carrying the underwhite (uw) mutation. A model for oculocutaneous albinism (OCA) type 4
    Gertrude E Costin
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 116:3203-12. 2003
    ..This mechanism explains the hypopigmentary phenotype of these cells and provides new insights into the involvement of transporters in the normal physiology of melanocytes...
  58. ncbi request reprint Epitope mapping of the melanosomal matrix protein gp100 (PMEL17): rapid processing in the endoplasmic reticulum and glycosylation in the early Golgi compartment
    Ken ichi Yasumoto
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Room 1B25, Building 37, Bethesda, MD 20892, USA
    J Biol Chem 279:28330-8. 2004
    ..Once this structural reorganization occurs, melanogenic enzymes begin to be targeted to the melanosomes, which are then competent to synthesize melanin pigment...
  59. ncbi request reprint Stimulation of melanoblast pigmentation by 8-methoxypsoralen:the involvement of microphthalmia-associated transcription factor, the protein kinase a signal pathway, and proteasome-mediated degradation
    Tie Chi Lei
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 119:1341-9. 2002
    ....
  60. ncbi request reprint MC1R and the response of melanocytes to ultraviolet radiation
    Francois Rouzaud
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 2132, Bethesda, MD 20892, USA
    Mutat Res 571:133-52. 2005
    ..The MC1R gene is considered a melanoma susceptibility gene, and its significance in determining the risk for skin cancer is of tremendous interest...
  61. ncbi request reprint Human melanocortin 1 receptor variants, receptor function and melanocyte response to UV radiation
    M Cathy Scott
    Department of Dermatology, University of Cincinnati College of Medicine, PO Box 670592, Cincinnati, Ohio 45267 0592, USA
    J Cell Sci 115:2349-55. 2002
    ..We conclude that loss-of-function mutations in the MC1R gene sensitize human melanocytes to the DNA damaging effects of UV radiation, which may increase skin cancer risk...
  62. ncbi request reprint The etiology of oculocutaneous albinism (OCA) type II: the pink protein modulates the processing and transport of tyrosinase
    Kazutomo Toyofuku
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Pigment Cell Res 15:217-24. 2002
    ..This study provides a basis to understand the relationship of the p protein with tyrosinase function and melanin synthesis, and also provides a rational approach to unveil the consequences of P gene mutations in the pathogenesis of OCA2...
  63. ncbi request reprint A melanocyte-keratinocyte coculture model to assess regulators of pigmentation in vitro
    Tie Chi Lei
    Pigment Cell Biology Section, Laboratory of Cell Biology, Bethesda, Maryland 20892, USA
    Anal Biochem 305:260-8. 2002
    ....
  64. ncbi request reprint Immortalization of mouse melanocytes carrying mutations in various pigmentation genes
    Gertrude E Costin
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 335:171-4. 2004
  65. pmc Melanin acts as a potent UVB photosensitizer to cause an atypical mode of cell death in murine skin
    Seiji Takeuchi
    Departments of Therapeutic Radiology, Genetics, and Dermatology, and Yale Comprehensive Cancer Center, Yale School of Medicine, New Haven, CT 06520 8040, USA
    Proc Natl Acad Sci U S A 101:15076-81. 2004
    ..Melanin-induced apoptosis may contribute to the increased sensitivity of individuals with blonde and red hair to sunburn and skin cancer...
  66. ncbi request reprint Reconstituted 3-dimensional human skin of various ethnic origins as an in vitro model for studies of pigmentation
    Tae Jin Yoon
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 318:260-9. 2003
    ....
  67. ncbi request reprint Dickkopf 1 (DKK1) regulates skin pigmentation and thickness by affecting Wnt/beta-catenin signaling in keratinocytes
    Yuji Yamaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    FASEB J 22:1009-20. 2008
    ....
  68. ncbi request reprint In vitro migration of melanoblasts requires matrix metalloproteinase-2: implications to vitiligo therapy by photochemotherapy
    Tie Chi Lei
    Pigment Cell Biology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Pigment Cell Res 15:426-32. 2002
    ..Taken together, these results suggest the importance of MMP2 in melanoblast migration and in the response to PUVA therapy...
  69. doi request reprint Isolation of melanosomes
    Hidenori Watabe
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2005
    ..Early melanosomes lacking pigment must be further purified using free-flow electrophoresis...
  70. ncbi request reprint Sialylated core 1 O-glycans influence the sorting of Pmel17/gp100 and determine its capacity to form fibrils
    Julio C Valencia
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:11266-80. 2007
    ..Alterations in sialyltransferase activity and substrates differ between normal and transformed melanocytes and may represent a critical change during malignant transformation...
  71. ncbi request reprint Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase
    Hideya Ando
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 127:751-61. 2007
    ....
  72. pmc Mutations in dopachrome tautomerase (Dct) affect eumelanin/pheomelanin synthesis, but do not affect intracellular trafficking of the mutant protein
    Gertrude E Costin
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH, Bethesda, MD 20892, USA
    Biochem J 391:249-59. 2005
    ..Thus the enzymatic activity of Dct may play a role in determining whether the eumelanin or pheomelanin pathway is preferred for pigment biosynthesis...
  73. ncbi request reprint Mesenchymal-epithelial interactions in the skin: aiming for site-specific tissue regeneration
    Yuji Yamaguchi
    Department of Dermatology, Osaka University Graduate School of Medicine, 2 2 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    J Dermatol Sci 40:1-9. 2005
    ..We review the importance of dermal-epidermal interactions in tissue homeostasis and regeneration, especially in palms and soles...
  74. ncbi request reprint MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes
    Toshihiko Hoashi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:14006-16. 2005
    ..We conclude that MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation...
  75. ncbi request reprint The repeat domain of the melanosomal matrix protein PMEL17/GP100 is required for the formation of organellar fibers
    Toshihiko Hoashi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 281:21198-208. 2006
    ..We conclude that the RPT domain is essential for its function in generating the fibrillar matrix of melanosomes and that the luminal domain is necessary for its correct processing and trafficking to those organelles...
  76. ncbi request reprint Pmel17: controversial indeed but critical to melanocyte function
    Julio C Valencia
    Pigment Cell Res 19:250-2; author reply 253-7. 2006
  77. pmc Melanosomal sequestration of cytotoxic drugs contributes to the intractability of malignant melanomas
    Kevin G Chen
    Laboratories of Cell Biology and Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:9903-7. 2006
    ..Preventing melanosomal sequestration of cytotoxic drugs by inhibiting the functions of melanosomes may have great potential as an approach to improving the chemosensitivity of melanoma cells...
  78. ncbi request reprint Influence of alpha-melanocyte-stimulating hormone and ultraviolet radiation on the transfer of melanosomes to keratinocytes
    Victoria M Virador
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH, Bethesda, Maryland, 20892, USA
    FASEB J 16:105-7. 2002
    ....
  79. ncbi request reprint Synergistic effect of interleukin-2 and a vaccine of irradiated melanoma cells transfected to secrete staphylococcal enterotoxin A
    David P Schrayer
    Department of Surgery and Pathology, University Medical Group Roger Williams Medical Center, Boston University School of Medicine, Providence, Rhode Island 02908, USA
    Clin Exp Metastasis 19:43-53. 2002
    ..The morphological and immunological effectiveness of the therapy was dose-dependent on IL-2...
  80. ncbi request reprint Regulation of melanocortin 1 receptor expression at the mRNA and protein levels by its natural agonist and antagonist
    Francois Rouzaud
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    FASEB J 17:2154-6. 2003
    ..Our study further shows that MSH regulates Mc1r function at both the mRNA and protein levels, whereas ASP acts only on its translation...
  81. ncbi request reprint Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes
    An Chi
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA
    J Proteome Res 5:3135-44. 2006
    ..The sharing of proteins between melanosomes and other lysosome-related organelles suggests a common evolutionary origin. This work represents a model for the study of the biogenesis of lysosome-related organelles...
  82. ncbi request reprint The effects of dickkopf 1 on gene expression and Wnt signaling by melanocytes: mechanisms underlying its suppression of melanocyte function and proliferation
    Yuji Yamaguchi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Invest Dermatol 127:1217-25. 2007
    ....
  83. ncbi request reprint Immunohistochemistry and in situ hybridization in the study of human skin melanocytes
    Thierry Passeron
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Exp Dermatol 16:162-70. 2007
    ..This methodology, along with relevant tips and troubleshooting items, are important tools to identify and study melanocytes in the skin...