John A Hanover

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint Bittersweet memories: linking metabolism to epigenetics through O-GlcNAcylation
    John A Hanover
    Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Rev Mol Cell Biol 13:312-21. 2012
  2. pmc Elevated O-GlcNAc-dependent signaling through inducible mOGT expression selectively triggers apoptosis
    Sang Hoon Shin
    Gyeong Gi Bio Center, Suwon, Korea
    Amino Acids 40:885-93. 2011
  3. ncbi request reprint Elevated O-linked N-acetylglucosamine metabolism in pancreatic beta-cells
    J A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Arch Biochem Biophys 362:38-45. 1999
  4. pmc Calmodulin-driven nuclear entry: trigger for sex determination and terminal differentiation
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, NIH, Bethesda, MD 20892, USA
    J Biol Chem 284:12593-7. 2009
  5. ncbi request reprint Glycan-dependent signaling: O-linked N-acetylglucosamine
    J A Hanover
    LCBB, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    FASEB J 15:1865-76. 2001
  6. pmc A Caenorhabditis elegans model of insulin resistance: altered macronutrient storage and dauer formation in an OGT-1 knockout
    John A Hanover
    Laboratories of Cell Biochemistry and Biology and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:11266-71. 2005
  7. pmc The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1800:80-95. 2010
  8. ncbi request reprint Mitochondrial and nucleocytoplasmic isoforms of O-linked GlcNAc transferase encoded by a single mammalian gene
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Building 8, Room 402, 8 Center Drive, MSC 0850, NIH, Bethesda, MD 20892 0850, USA
    Arch Biochem Biophys 409:287-97. 2003
  9. pmc Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer
    Michele E Forsythe
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0850, USA
    Proc Natl Acad Sci U S A 103:11952-7. 2006
  10. ncbi request reprint The High Mobility Group Box Transcription Factor Nhp6Ap enters the nucleus by a calmodulin-dependent, Ran-independent pathway
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    J Biol Chem 282:33743-51. 2007

Collaborators

Detail Information

Publications57

  1. doi request reprint Bittersweet memories: linking metabolism to epigenetics through O-GlcNAcylation
    John A Hanover
    Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Rev Mol Cell Biol 13:312-21. 2012
    ..The wide range of physiological functions regulated by O-GlcNAc cycling suggests an unexplored nexus between epigenetic regulation in disease and nutrient availability...
  2. pmc Elevated O-GlcNAc-dependent signaling through inducible mOGT expression selectively triggers apoptosis
    Sang Hoon Shin
    Gyeong Gi Bio Center, Suwon, Korea
    Amino Acids 40:885-93. 2011
    ..These studies suggest that deregulated activity of the mitochondrially targeted mOGT may play a role in triggering the programmed cell death observed with diseases such as diabetes mellitus and neurodegeneration...
  3. ncbi request reprint Elevated O-linked N-acetylglucosamine metabolism in pancreatic beta-cells
    J A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Arch Biochem Biophys 362:38-45. 1999
    ..Taken together, these findings suggest that pancreatic beta cells maintain a highly elevated O-GlcNAc metabolism and that the diabetes inducing drug streptozotocin inhibits O-GlcNAcase...
  4. pmc Calmodulin-driven nuclear entry: trigger for sex determination and terminal differentiation
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, NIH, Bethesda, MD 20892, USA
    J Biol Chem 284:12593-7. 2009
    ..Ca(2+)-calmodulin-triggered nuclear entry of key architectural transcription factors is a potentially key epigenetic regulator of terminal differentiation in response to cell signaling...
  5. ncbi request reprint Glycan-dependent signaling: O-linked N-acetylglucosamine
    J A Hanover
    LCBB, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    FASEB J 15:1865-76. 2001
    ..Altered O-linked GlcNAc metabolism may also occur in human diseases including neurodegenerative disorders, diabetes mellitus and cancer...
  6. pmc A Caenorhabditis elegans model of insulin resistance: altered macronutrient storage and dauer formation in an OGT-1 knockout
    John A Hanover
    Laboratories of Cell Biochemistry and Biology and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:11266-71. 2005
    ..Our findings demonstrate that OGT modulates macronutrient storage and dauer formation in C. elegans, providing a unique genetic model for examining the role of O-GlcNAc in cellular signaling and insulin resistance...
  7. pmc The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1800:80-95. 2010
    ..It will emphasize the impact O-GlcNAcylation has upon signaling pathways that may be become deregulated in diseases of the immune system, diabetes mellitus, cancer, cardiovascular disease, and neurodegenerative diseases...
  8. ncbi request reprint Mitochondrial and nucleocytoplasmic isoforms of O-linked GlcNAc transferase encoded by a single mammalian gene
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Building 8, Room 402, 8 Center Drive, MSC 0850, NIH, Bethesda, MD 20892 0850, USA
    Arch Biochem Biophys 409:287-97. 2003
    ..These results provide the basis for a more detailed analysis of the significance and regulation of the nucleocytoplasmic and mitochondrial isoforms of OGT in mammals...
  9. pmc Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer
    Michele E Forsythe
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0850, USA
    Proc Natl Acad Sci U S A 103:11952-7. 2006
    ..The knockout of O-GlcNAcase (oga-1) in C. elegans mimics many of the metabolic and signaling changes associated with human insulin resistance and provides a genetically amenable model of non-insulin-dependent diabetes...
  10. ncbi request reprint The High Mobility Group Box Transcription Factor Nhp6Ap enters the nucleus by a calmodulin-dependent, Ran-independent pathway
    John A Hanover
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0851, USA
    J Biol Chem 282:33743-51. 2007
    ..The finding that Nhp6Ap nuclear entry requires calmodulin but not Ran indicates that Nhp6Ap is a good model for studying this poorly understood but evolutionarily conserved calmodulin-dependent nuclear import pathway...
  11. pmc Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling
    Fumihiko Furuya
    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr, Room 5128, Bethesda, MD 20892 4264, USA
    Mol Cell Biol 27:6116-26. 2007
    ..Taken together, these results indicate that NCoR, via protein-protein interaction, is a novel regulator of PI3K signaling and could serve to modulate thyroid tumor progression...
  12. ncbi request reprint Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate
    Eun Ju Kim
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, MD 20892, USA
    Carbohydr Res 341:971-82. 2006
    ..The finding that O-GlcNAcase exists as two distinct isoforms has a number of important implications for the role of O-GlcNAcase in hexosamine signaling...
  13. ncbi request reprint Recombinant O-GlcNAc transferase isoforms: identification of O-GlcNAcase, yes tyrosine kinase, and tau as isoform-specific substrates
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, MD 20897 0851, USA
    Glycobiology 16:415-21. 2006
    ..The identification of a tyrosine kinase and O-GlcNAcase as OGT targets suggests the potential for OGT participation in numerous signal transduction cascades...
  14. pmc Evidence of the involvement of O-GlcNAc-modified human RNA polymerase II CTD in transcription in vitro and in vivo
    Stella M Ranuncolo
    Metabolism Branch, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:23549-61. 2012
    ..Our results define unexpected roles for both the CTD and O-GlcNAc in the regulation of transcription initiation in higher eukaryotes...
  15. ncbi request reprint Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 116:647-54. 2003
    ..The differential localization of mitochondrial and nucleocytoplasmic isoforms of OGT suggests that they perform unique intracellular functions...
  16. ncbi request reprint Gelsolin: a novel thyroid hormone receptor-beta interacting protein that modulates tumor progression in a mouse model of follicular thyroid cancer
    Caroline S Kim
    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, 37 Convent Drive, Bethesda, MD 20892 4264, USA
    Endocrinology 148:1306-12. 2007
    ..Thus, the present study uncovered a novel PV-mediated oncogenic pathway that could contribute to the local tumor progression and metastatic potential of thyroid carcinogenesis...
  17. ncbi request reprint An O-GlcNAcase-specific inhibitor and substrate engineered by the extension of the N-acetyl moiety
    Eun Ju Kim
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Am Chem Soc 128:4234-5. 2006
    ..This reagent provides, for the first time, a means for monitoring O-GlcNAcase activity independent of the related enzymes hexosaminidase A and hexosaminidase B...
  18. pmc O-GlcNAc cycling mutants modulate proteotoxicity in Caenorhabditis elegans models of human neurodegenerative diseases
    Peng Wang
    Laboratories of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:17669-74. 2012
    ..These findings suggest that O-GlcNAc levels may directly influence neurodegenerative disease progression, thus making the enzymes of O-GlcNAc cycling attractive targets for neurodegenerative disease therapies...
  19. ncbi request reprint Mutational analysis of the catalytic domain of O-linked N-acetylglucosaminyl transferase
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:35537-44. 2005
    ..Identification of viable OGT mutants may facilitate examination of its role in nutrient sensing and signal transduction cascades...
  20. ncbi request reprint Tumor necrosis factor receptor 2 signaling induces selective c-IAP1-dependent ASK1 ubiquitination and terminates mitogen-activated protein kinase signaling
    Yongge Zhao
    Laboratory of Immune Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:7777-82. 2007
    ..Moreover, in the absence of c-IAP1 TNFR2-mediated p38 and JNK activation was prolonged. Thus, the ubiquitin protein ligase activity of c-IAP1 is responsible for regulating the duration of TNF signaling in primary cells expressing TNFR2...
  21. ncbi request reprint The hexosamine signaling pathway: deciphering the "O-GlcNAc code"
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Sci STKE 2005:re13. 2005
    ..This ongoing effort requires analysis of the enzymes of O-GlcNAc metabolism, their many targets, and how the O-GlcNAc modification may be regulated...
  22. pmc Aberrant accumulation of PTTG1 induced by a mutated thyroid hormone beta receptor inhibits mitotic progression
    Hao Ying
    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 116:2972-84. 2006
    ..The loss of this regulatory function in PV led to an aberrant accumulation of PTTG1 disrupting mitotic progression that could contribute to thyroid carcinogenesis...
  23. pmc O-GlcNAc cycling: implications for neurodegenerative disorders
    Brooke D Lazarus
    Laboratory of Cell Biology and Biochemistry, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Biochem Cell Biol 41:2134-46. 2009
    ..Finally, we will focus attention on the many ways by which O-GlcNAc cycling may affect the cellular machinery in the neuroendocrine and central nervous systems...
  24. pmc Dynamic O-GlcNAc cycling at promoters of Caenorhabditis elegans genes regulating longevity, stress, and immunity
    Dona C Love
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0851, USA
    Proc Natl Acad Sci U S A 107:7413-8. 2010
    ..The observed impact of O-GlcNAc cycling on both signaling and transcription in C. elegans has important implications for human diseases of aging, including diabetes and neurodegeneration...
  25. pmc Nutrient-driven O-GlcNAc cycling influences autophagic flux and neurodegenerative proteotoxicity
    Peng Wang
    Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Autophagy 9:604-6. 2013
    ..We speculate that O-GlcNAc cycling is a key nutrient-responsive regulator of autophagic flux acting at multiple levels including direct modification of BECN1 and BCL2...
  26. pmc O-GlcNAc cycling: emerging roles in development and epigenetics
    Dona C Love
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, NIH, Bethesda, MD 20892 0850, USA
    Semin Cell Dev Biol 21:646-54. 2010
    ....
  27. ncbi request reprint Distinctive inhibition of O-GlcNAcase isoforms by an alpha-GlcNAc thiolsulfonate
    Eun J Kim
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institute of Health, Bethesda, Maryland 20892, USA
    J Am Chem Soc 129:14854-5. 2007
    ..Covalent attachment of GlcNAc to the HAT domain of long OGA dramatically changes its properties with respect to enzymatic activity and caspase-3 cleavage...
  28. pmc A lipid-droplet-targeted O-GlcNAcase isoform is a key regulator of the proteasome
    Chithra N Keembiyehetty
    Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Kidney Diseases, National Institute of Health, Bethesda, MD 20892, USA
    J Cell Sci 124:2851-60. 2011
    ..Our findings therefore suggest a mechanistic link between hexosamine signaling and lipid droplet assembly and mobilization...
  29. doi request reprint O-GlcNAc cycling: a link between metabolism and chronic disease
    Michelle R Bond
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Annu Rev Nutr 33:205-29. 2013
    ....
  30. pmc Activation of phosphatidylinositol 3-kinase signaling by a mutant thyroid hormone beta receptor
    Fumihiko Furuya
    Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 4264, USA
    Proc Natl Acad Sci U S A 103:1780-5. 2006
    ..Importantly, the present study unveils a mechanism by which a mutant TR acts to activate PI3K activity via protein-protein interactions...
  31. pmc Blocking O-linked GlcNAc cycling in Drosophila insulin-producing cells perturbs glucose-insulin homeostasis
    Osamu Sekine
    Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:38684-91. 2010
    ..The observed phenotypes in O-GlcNAc cycling mimic pancreatic β-cell dysfunction and glucose toxicity related to sustained hyperglycemia in mammals...
  32. ncbi request reprint Insulin inhibition of transcription stimulated by the forkhead protein Foxo1 is not solely due to nuclear exclusion
    Wen Chi Tsai
    Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health, Bethesda, Maryland 20892 1758, USA
    Endocrinology 144:5615-22. 2003
    ....
  33. ncbi request reprint Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry
    Melissa Perreira
    Chemical Biology Core Facility, National Institute of Diabetes and Digestive and Kidney Disorders, National Institutes of Health, Bethesda, MD 20892, USA
    Bioorg Med Chem 14:837-46. 2006
    ..It was determined via both in vitro and intact cell experiments that the Z form of PUGNAc was vastly more potent an inhibitor of O-GlcNAcase than the E form...
  34. pmc A convenient synthesis of the C-1-phosphonate analogue of UDP-GlcNAc and its evaluation as an inhibitor of O-linked GlcNAc transferase (OGT)
    Jan Hajduch
    Laboratory of Bioorganic Chemistry, National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, DHHS, Bethesda, MD 20892, United States
    Carbohydr Res 343:189-95. 2008
    ..Deoxygenation, coupling of the resulting phosphonic acid with UMP and deprotection gave the target molecule as a di-sodium salt. This analogue had no detectable activity as an inhibitor of (OGT)...
  35. pmc Crp79p, like Mex67p, is an auxiliary mRNA export factor in Schizosaccharomyces pombe
    Anjan G Thakurta
    Basic Research Laboratory, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 13:2571-84. 2002
    ..We propose that Crp79p is a nonessential mRNA export carrier in S. pombe...
  36. ncbi request reprint The Stat3/5 locus encodes novel endoplasmic reticulum and helicase-like proteins that are preferentially expressed in normal and neoplastic mammary tissue
    Y Cui
    Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genomics 78:129-34. 2001
    ..Immunofluorescence studies demonstrated that LGP1 is located in the nuclear envelope and the endoplasmic reticulum. LGP2 is a cytoplasmic protein of 678 amino acids...
  37. ncbi request reprint The long signal peptide isoform and its alternative processing direct the intracellular trafficking of interleukin-15
    G Kurys
    Metabolism Branch, NCI, National Institutes of Health and the Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1374, USA
    J Biol Chem 275:30653-9. 2000
    ..In terms of a more positive role, we propose that intracellular infection may relieve the burdens on translation and intracellular trafficking to yield effective IL-15 expression...
  38. pmc Partial cDNA sequence encoding a nuclear pore protein modified by O-linked N-acetylglucosamine
    M D'Onofrio
    Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 85:9595-9. 1988
    ..The partial sequence of the 62-kDa nuclear pore glycoprotein shows little similarity to other characterized proteins and elucidates structural features of a member of the family of nuclear pore glycoproteins...
  39. pmc Generation of secretable and nonsecretable interleukin 15 isoforms through alternate usage of signal peptides
    Y Tagaya
    Metabolism Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:14444-9. 1997
    ..Such production of an intracellular lymphokine is not typical of other soluble interleukin systems, suggesting a biological function for IL-15 as an intracellular molecule...
  40. ncbi request reprint Nuclear glycogen and glycogen synthase kinase 3
    M Ragano-Caracciolo
    Laboratory of Cell Biochemestry and Biology LCBB, National Institute of Diabetes and Kidney and Digestive Disease, National Institutes of Health, 8 Center Drive, Bethesda, Maryland, 20892 MSC 0851, USA
    Biochem Biophys Res Commun 249:422-7. 1998
    ....
  41. pmc TNF-alpha induced c-IAP1/TRAF2 complex translocation to a Ubc6-containing compartment and TRAF2 ubiquitination
    Chuan Jin Wu
    Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 24:1886-98. 2005
    ..Therefore, the ER plays a key role in the TNF-R-mediated signal transduction cascade by acting as a site of assembly for E2/E3/substrate complexes...
  42. doi request reprint Enzymatic characterization of recombinant enzymes of O-GlcNAc cycling
    Eun Ju Kim
    Laboratory Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 1022:129-45. 2013
    ..Here we describe the production of and methods for assay of the recombinant enzymes of O-GlcNAc cycling: O-linked GlcNAc Transferase (OGT) and O-GlcNAcase (OGA)...
  43. pmc The hydroxyurea-induced small GTP-binding protein SAR modulates gamma-globin gene expression in human erythroid cells
    Delia C Tang
    Bldg 10, Rm 9N119, Molecular and Clinical Hematology Branch and Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 106:3256-63. 2005
    ..We suggest that SAR may participate in both erythroid cell growth and gamma-globin production by regulating PI3 kinase/extracellular protein-related kinase (ERK) and GATA-2/p21-dependent signal transduction pathways...
  44. ncbi request reprint The gene encoding rat nuclear pore glycoprotein p62 is intronless
    M D'Onofrio
    Laboratory of Biochemistry and Metabolism, National Institutes of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 266:11980-5. 1991
    ..When linked to a reporter gene, the 5'-flanking region of the p62 gene serves as an active promoter...
  45. ncbi request reprint Organization of the mouse ASGR1 gene encoding the major subunit of the hepatic asialoglycoprotein receptor
    S Soukharev
    Laboratory of Biochemistry and Metabolism, National Institute of Diabetes, Digestive and Kidney Disease, Bethesda, MD 20892, USA
    Gene 241:233-40. 2000
    ..These results provide the basis for more detailed genetic studies on the functional role of the hepatic asialoglycoprotein receptor in mammals...
  46. pmc Primary sequence and heterologous expression of nuclear pore glycoprotein p62
    C M Starr
    Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    J Cell Biol 110:1861-71. 1990
    ..The ability to express p62 in tissue culture cells will facilitate analysis of the role of this pore protein in nuclear transport...
  47. ncbi request reprint MLN64 mediates mobilization of lysosomal cholesterol to steroidogenic mitochondria
    Mei Zhang
    Lipid Cell Biology Section and Cell Biochemistry Section, Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:33300-10. 2002
    ..We conclude that MLN64 participates in mobilization and utilization of lysosomal cholesterol by virtue of the START domain's role in cholesterol transport...
  48. ncbi request reprint Hormone-induced translocation of thyroid hormone receptors in living cells visualized using a receptor green fluorescent protein chimera
    X G Zhu
    Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:27058-63. 1998
    ..Furthermore, the inability of the mutant TR to translocate to the nucleus correlated with the loss of most of its transcriptional activity. These results suggest that TR functions may, in part, be regulated by T3-induced nuclear entry...
  49. ncbi request reprint Clathrin assembly protein AP-3 is phosphorylated and glycosylated on the 50-kDa structural domain
    J E Murphy
    Laboratory of Biochemistry and Metabolism, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:21346-52. 1994
    ..Consistent with localization to the nonclathrin binding domain, the O-GlcNAc modification does not play a discernible role in the interaction of AP-3 with clathrin...
  50. pmc Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export
    J H Yoon
    Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 20:8767-82. 2000
    ..Finally, we propose that the 149-505 region of spMex67p could act as an accessory factor in Rae1p-dependent transport and that spMex67p participates at various common steps with Rae1p export complexes in promoting the export of mRNA...
  51. pmc Cessation of rapid late endosomal tubulovesicular trafficking in Niemann-Pick type C1 disease
    M Zhang
    Lipid Cell Biology Section and Cell Biochemistry Section, National Institute of Diabetes and Digestive and Kidney Diseases, and Developmental and Metabolic Neurology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:4466-71. 2001
    ..We conclude that the neuropathology and cellular lysosomal lipid accumulation in NPC1 disease results, at least in part, from striking defects in late endosomal tubulovesicular trafficking...
  52. ncbi request reprint An isoform of branched-chain aminotransferase is a novel co-repressor for thyroid hormone nuclear receptors
    H M Lin
    Gene Regulation Section, Laboratory of Molecular Biology, NCI and Laboratory of Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0851, USA
    J Biol Chem 276:48196-205. 2001
    ..Thus, unlike the currently known co-repressors, P3 is a novel ligand-independent co-repressor for TR...
  53. ncbi request reprint A versatile sugar transferase makes the cut
    John A Hanover
    Laboratory of Cellular and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 0851, USA
    Cell 144:321-3. 2011
    ....
  54. pmc Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia
    Donald A McClain
    Department of Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 99:10695-9. 2002
    ..These data support the proposal that O-linked GlcNAc transferase participates in a hexosamine-dependent signaling pathway that is linked to insulin resistance and leptin production...
  55. pmc A chemical approach for identifying O-GlcNAc-modified proteins in cells
    David J Vocadlo
    Center for New Directions in Organic Synthesis, Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 100:9116-21. 2003
    ..This strategy will prove useful for both the identification of O-GlcNAc-modified proteins and the elucidation of the specific residues that bear this saccharide...
  56. ncbi request reprint The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin alpha
    Martin Jinek
    Structural and Computational Biology, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Struct Mol Biol 11:1001-7. 2004
    ..Based on this structural similarity, we propose that OGT uses an analogous molecular mechanism to recognize its targets...
  57. ncbi request reprint Tautomeric modification of GlcNAc-thiazoline
    Spencer Knapp
    Department of Chemistry and Chemical Biology, Rutgers The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, USA
    Org Lett 9:2321-4. 2007
    ..A new radical addition/fragmentation reaction of the N-(trifluoroacetyl)enamine has been discovered...