G L Hager

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Rapid genome-scale mapping of chromatin accessibility in tissue
    Lars Grøntved
    Laboratory of Receptor Biology and Gene Expression, 41 Library Dr, National Cancer Institute, Building 41 B602, Bethesda, MD, 20892, USA
    Epigenetics Chromatin 5:10. 2012
  2. ncbi request reprint Dynamics of gene targeting and chromatin remodelling by nuclear receptors
    G L Hager
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Biochem Soc Trans 28:405-10. 2000
  3. ncbi request reprint Protein dynamics in the nuclear compartment
    Gordon L Hager
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Curr Opin Genet Dev 12:137-41. 2002
  4. ncbi request reprint Chromatin dynamics and the evolution of alternate promoter states
    Gordon L Hager
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, NIH, Building 41, Room B602, Bethesda, MD 20892 5055, USA
    Chromosome Res 14:107-16. 2006
  5. pmc Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter
    W G Muller
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, 41 Library Dr, Bethesda, MD 20892, USA
    J Cell Biol 154:33-48. 2001
  6. ncbi request reprint The glucocorticoid receptor: rapid exchange with regulatory sites in living cells
    J G McNally
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Science 287:1262-5. 2000
  7. ncbi request reprint Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras
    C S Lim
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, Maryland 20892 5055, USA
    Mol Endocrinol 13:366-75. 1999
  8. ncbi request reprint Intranuclear trafficking and gene targeting by members of the steroid/nuclear receptor superfamily
    G L Hager
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892 5055, USA
    J Steroid Biochem Mol Biol 65:125-32. 1998
  9. ncbi request reprint Nuclear cytoplasmic shuttling by thyroid hormone receptors. multiple protein interactions are required for nuclear retention
    C T Baumann
    Laboratory of Receptor Biology and Gene Expression, NCI and Molecular Regulation and Neuroendocrinology Section, Clinical Endocrinology Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:11237-45. 2001
  10. ncbi request reprint The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome
    C T Baumann
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health Bethesda, Maryland 20892-5055, USA
    Mol Endocrinol 15:485-500. 2001

Collaborators

Detail Information

Publications66

  1. pmc Rapid genome-scale mapping of chromatin accessibility in tissue
    Lars Grøntved
    Laboratory of Receptor Biology and Gene Expression, 41 Library Dr, National Cancer Institute, Building 41 B602, Bethesda, MD, 20892, USA
    Epigenetics Chromatin 5:10. 2012
    ..abstract:..
  2. ncbi request reprint Dynamics of gene targeting and chromatin remodelling by nuclear receptors
    G L Hager
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Biochem Soc Trans 28:405-10. 2000
    ..The results both in vitro and in vivo are consistent with a dynamic model ('hit and run') in which GR first binds to chromatin after ligand activation, recruits a remodelling activity and is then lost from the template...
  3. ncbi request reprint Protein dynamics in the nuclear compartment
    Gordon L Hager
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Curr Opin Genet Dev 12:137-41. 2002
    ..Two countervailing views of factor/regulatory site interactions emerge from the current literature...
  4. ncbi request reprint Chromatin dynamics and the evolution of alternate promoter states
    Gordon L Hager
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, NIH, Building 41, Room B602, Bethesda, MD 20892 5055, USA
    Chromosome Res 14:107-16. 2006
    ..Potential mechanisms involved in the unexpectedly rapid flux of factor/template interactions are discussed in the context of a "return-to-template" model for transcription factor function...
  5. pmc Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter
    W G Muller
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, 41 Library Dr, Bethesda, MD 20892, USA
    J Cell Biol 154:33-48. 2001
    ..They also support fiber-packing models of higher order structure and suggest that transcription from a natural promoter may occur at much higher DNA-packing densities than reported previously...
  6. ncbi request reprint The glucocorticoid receptor: rapid exchange with regulatory sites in living cells
    J G McNally
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Science 287:1262-5. 2000
    ..Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed...
  7. ncbi request reprint Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras
    C S Lim
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, Maryland 20892 5055, USA
    Mol Endocrinol 13:366-75. 1999
    ..The causes for and implications of this differential localization of the A and B forms of the human PR are discussed...
  8. ncbi request reprint Intranuclear trafficking and gene targeting by members of the steroid/nuclear receptor superfamily
    G L Hager
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892 5055, USA
    J Steroid Biochem Mol Biol 65:125-32. 1998
    ....
  9. ncbi request reprint Nuclear cytoplasmic shuttling by thyroid hormone receptors. multiple protein interactions are required for nuclear retention
    C T Baumann
    Laboratory of Receptor Biology and Gene Expression, NCI and Molecular Regulation and Neuroendocrinology Section, Clinical Endocrinology Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:11237-45. 2001
    ....
  10. ncbi request reprint The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome
    C T Baumann
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health Bethesda, Maryland 20892-5055, USA
    Mol Endocrinol 15:485-500. 2001
    ..These findings suggest the intriguing possibility that degradation of GRIP1 by the 26S proteasome may be a key component of its regulation...
  11. ncbi request reprint Characterization of transiently and constitutively expressed progesterone receptors: evidence for two functional states
    C L Smith
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Endocrinol 14:956-71. 2000
    ....
  12. ncbi request reprint A glucocorticoid/retinoic acid receptor chimera that displays cytoplasmic/nuclear translocation in response to retinoic acid. A real time sensing assay for nuclear receptor ligands
    S Mackem
    Laboratory of Pathology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:45501-4. 2001
    ....
  13. ncbi request reprint Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure
    C L Smith
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    J Biol Chem 272:14227-35. 1997
    ..These differences may provide a mechanism for establishing target gene specificity in vivo for steroid receptors that recognize and bind to identical DNA sequences...
  14. ncbi request reprint Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser
    T S Karpova
    CCR Core Fluorescence Imaging Facility, Laboratory of Receptor Biology and Gene Expression, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Microsc 209:56-70. 2003
    ..In summary, we extensively characterize a simple approach to FRET that should be adaptable to most laser-scanning confocal microscopes, and demonstrate its feasibility for detecting FRET between several CFP/YFP partners...
  15. pmc Molecular chaperones function as steroid receptor nuclear mobility factors
    Cem Elbi
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 5055, USA
    Proc Natl Acad Sci U S A 101:2876-81. 2004
    ..Thus, our results provide a description of soluble nuclear mobility factors and furthermore demonstrate a previously unrecognized role for molecular chaperones in the regulation of steroid receptor function within the nucleus...
  16. pmc Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro
    Geetha V Rayasam
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, National Cancer Institute, National Institutes of Health, 41 Library Dr, Bethesda, MD 20892 5055, USA
    Mol Cell Biol 25:2406-18. 2005
    ....
  17. ncbi request reprint A novel in situ assay for the identification and characterization of soluble nuclear mobility factors
    Cem Elbi
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Sci STKE 2004:pl10. 2004
    ..This assay can readily be adapted to identify and characterize other nuclear mobility factors...
  18. ncbi request reprint Selective intranuclear redistribution of PPAR isoforms by RXR alpha
    Taro E Akiyama
    Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Endocrinol 16:707-21. 2002
    ..These results implicate RXR alpha in the nuclear reorganization of PPAR gamma and suggest that PPAR gamma colocalizes with RXR alpha at specific locations within the nucleus independent of added ligand...
  19. ncbi request reprint Characterization of an NF-1/CTF family member as a functional activator of the mouse mammary tumor virus long terminal repeat 5' enhancer
    P Kusk
    Laboratory of Receptor Biology and Gene Expression, NCI, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    J Biol Chem 271:31269-76. 1996
    ..We conclude that the F3 activity represents a new member of the NF-1/CTF family...
  20. ncbi request reprint Hormone-induced translocation of thyroid hormone receptors in living cells visualized using a receptor green fluorescent protein chimera
    X G Zhu
    Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:27058-63. 1998
    ..Furthermore, the inability of the mutant TR to translocate to the nucleus correlated with the loss of most of its transcriptional activity. These results suggest that TR functions may, in part, be regulated by T3-induced nuclear entry...
  21. doi request reprint Visualizing chromatin dynamics in intact cells
    Ty C Voss
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, National Cancer Institute, NIH Bethesda, MD 20892 5055, USA
    Biochim Biophys Acta 1783:2044-51. 2008
    ..In this review we discuss how dynamic interactions allow transcriptional regulatory proteins find their targets within the nucleus, alter target chromatin structure, and modulate physiological gene expression...
  22. doi request reprint Interaction of the glucocorticoid receptor with the chromatin landscape
    Sam John
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Mol Cell 29:611-24. 2008
    ..Furthermore, the widespread requirement for chromatin remodeling supports the recent hypothesis that the rapid exchange of receptor proteins occurs during nucleosome reorganization...
  23. pmc Dynamic access of the glucocorticoid receptor to response elements in chromatin
    Anuja A George
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Int J Biochem Cell Biol 41:214-24. 2009
    ..In this review we discuss the dynamics of a nuclear receptor, and its transcriptional response in the chromatin context...
  24. ncbi request reprint UV laser cross-linking: a real-time assay to study dynamic protein/DNA interactions during chromatin remodeling
    Akhilesh K Nagaich
    Laboratory of Receptor Biology and Gene Expression, Bldg 41 Room B602, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Sci STKE 2004:pl13. 2004
    ....
  25. ncbi request reprint Dynamics of nuclear receptor movement and transcription
    Gordon L Hager
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Building 41, Room B602, 41 Library Dr MSC 5055, Bethesda, MD 20892 5055, USA
    Biochim Biophys Acta 1677:46-51. 2004
    ..Several processes are implicated in rapid nuclear dynamics, including potential roles for molecular chaperones, the proteasome degradation machinery and chromatin remodeling complexes...
  26. pmc Combinatorial probabilistic chromatin interactions produce transcriptional heterogeneity
    Ty C Voss
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Cell Sci 122:345-56. 2009
    ....
  27. pmc Sustained oscillations of NF-kappaB produce distinct genome scanning and gene expression profiles
    Myong Hee Sung
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 4:e7163. 2009
    ..We propose that negative feedback loops do not simply terminate signaling, but rather promote oscillations of NF-kappaB in the nucleus, and these oscillations are functionally advantageous...
  28. ncbi request reprint Dynamic shuttling and intranuclear mobility of nuclear hormone receptors
    Padma Maruvada
    Molecular Regulation and Neuroendocrinology Section, Clinical Endocrinology Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:12425-32. 2003
    ..They also show that ligand-binding and protein-protein interactions can affect the intracellular mobility of some NRs and thereby may contribute to their biological activity...
  29. pmc Chromatin remodeling complexes interact dynamically with a glucocorticoid receptor-regulated promoter
    Thomas A Johnson
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 5055, USA
    Mol Biol Cell 19:3308-22. 2008
    ..Our data provide a kinetic and mechanistic basis for the BRG1 and BRM chromatin-remodeling complexes in regulating gene expression at a steroid hormone inducible promoter...
  30. pmc Dynamic effect of bortezomib on nuclear factor-kappaB activity and gene expression in tumor cells
    Myong Hee Sung
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Room B602, Bldg 41, 41 Library Dr, Bethesda, MD 20892, USA
    Mol Pharmacol 74:1215-22. 2008
    ....
  31. doi request reprint Transcription dynamics
    Gordon L Hager
    National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 35:741-53. 2009
    ..Its dynamic nature is not only a fundamental property of the transcription machinery, but it is emerging as an important modulator of physiological processes, particularly in differentiation and development...
  32. ncbi request reprint Characterization of the human glucocorticoid receptor promoter
    Y Nobukuni
    Clinical Neurogenetics Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 34:8207-14. 1995
    ..Our studies demonstrate that several transcription factors are involved in regulating GR expression and that AP2 could function as an important positive regulator of GR promoter activity...
  33. ncbi request reprint Rapid periodic binding and displacement of the glucocorticoid receptor during chromatin remodeling
    Akhilesh K Nagaich
    Laboratory of Receptor Biology and Gene Expression, Building 41, B602, 41 Library Drive, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 14:163-74. 2004
    ..These results indicate that enhancement of hSWI/SNF-mediated factor accessibility, a hallmark of chromatin remodeling, is in some cases transient, reversible, and periodic...
  34. ncbi request reprint Subnuclear trafficking and gene targeting by steroid receptors
    Akhilesh K Nagaich
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 5055, USA
    Ann N Y Acad Sci 1024:213-20. 2004
    ..We also find that receptor mobility in the nucleoplasm is strongly enhanced by molecular chaperones. These observations indicate that multiple mechanisms are involved in transient receptor interactions with nucleoplasmic targets...
  35. doi request reprint Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription
    Diana A Stavreva
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Nat Cell Biol 11:1093-102. 2009
    ..The GR signalling pathway has been optimized for a prompt and timely response to fluctuations in hormone levels, indicating that biologically accurate regulation of gene targets by GR requires an ultradian mode of hormone stimulation...
  36. ncbi request reprint Development of assays for nuclear receptor modulators using fluorescently tagged proteins
    Elisabeth D Martinez
    Laboratory of Receptor Biology and Gene Expression, Hormone Action and Oncogenesis Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Methods Enzymol 414:37-50. 2006
    ..This approach has been validated for both the estrogen receptor and the retinoic acid receptor and should be applicable to any member of the superfamily, facilitating the identification of new ligands and selective receptor modulators...
  37. pmc Recruitment of dioxin receptor to active transcription sites
    Cem Elbi
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Mol Biol Cell 13:2001-15. 2002
    ..These results indicate that AhR/ARNT complexes are recruited to specific subnuclear compartments in a ligand-dependent manner and that these foci represent the sites of AhR target genes...
  38. pmc ATP-dependent mobilization of the glucocorticoid receptor during chromatin remodeling
    Terace M Fletcher
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Mol Cell Biol 22:3255-63. 2002
    ....
  39. pmc Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes
    Diana A Stavreva
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute Light Imaging Facility, National Institute for Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA
    Mol Cell Biol 24:2682-97. 2004
    ..Moreover, our results reveal that longer GR residence times are consistently associated with greater transcriptional output, suggesting a new paradigm in which the rate of rapid exchange provides a means to tune transcriptional levels...
  40. ncbi request reprint High-yield purification of functionally active glucocorticoid receptor
    T M Fletcher
    Laboratory of Receptor Biology and Gene Expression, NCI, NIH, Bethesda, MD, USA
    Methods Mol Biol 176:55-65. 2001
  41. doi request reprint Glucocorticoid receptor dynamics and gene regulation
    Simon C Biddie
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, MD 20892 5055, USA
    Stress 12:193-205. 2009
    ..This finding has led to a paradigm shift in our understanding of receptor function throughout the genome. The mechanisms involved in these rapid exchange events, as well as the implications for receptor function, are discussed...
  42. pmc Glucocorticoid receptor activation of the Ciz1-Lcn2 locus by long range interactions
    Ofir Hakim
    Laboratory of Receptor Biology and Gene Expression, NCI, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    J Biol Chem 284:6048-52. 2009
    ..The strong correlation between gene expression and loop structure in different cell lines suggests that high order interactions play a role in determining tissue-specific gene regulation...
  43. pmc Kinetic complexity of the global response to glucocorticoid receptor action
    Sam John
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Endocrinology 150:1766-74. 2009
    ..Thus, the cellular response to GR stimulation involves a highly orchestrated series of regulatory actions and not simply a binary response to hormone...
  44. ncbi request reprint Effects of chromatin structure on the enzymatic and DNA binding functions of DNA methyltransferases DNMT1 and Dnmt3a in vitro
    Andrea K Robertson
    Epigenetic Gene Regulation and Cancer Section, LRBGE NCI NIH, Bldg 41, Rm C306, 41 Library Dr, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 322:110-8. 2004
    ..These findings raise interesting implications about the interactions of mammalian DNA methyltransferases with chromatin and provide the first evidence that a chromatin remodeling enzyme can alter the biological properties of a DNMT...
  45. ncbi request reprint HDAC1 acetylation is linked to progressive modulation of steroid receptor-induced gene transcription
    Yi Qiu
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Building 41, B602, Bethesda, Maryland 20892, USA
    Mol Cell 22:669-79. 2006
    ..These findings indicate that HDAC1 is required for the induction of some genes by the GR, and this activator function is dynamically modulated by acetylation...
  46. pmc 3D shortcuts to gene regulation
    Ofir Hakim
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 22:305-13. 2010
    ..Thus the spatial architecture of the genome has attracted a lot of interest and has intensified its significance in modern cell biology. Here we discuss current methods, concepts, and controversies in this rapidly evolving field...
  47. pmc Genome-wide mechanisms of nuclear receptor action
    Simon C Biddie
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, 41 Library Dr, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 5055, USA
    Trends Endocrinol Metab 21:3-9. 2010
    ..These mechanisms contribute importantly to the action of nuclear receptors in health and disease...
  48. ncbi request reprint An estrogen receptor chimera senses ligands by nuclear translocation
    Elisabeth D Martinez
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health NIH, Bethesda, MD 20892 5055, USA
    J Steroid Biochem Mol Biol 97:307-21. 2005
    ..The translocation properties of this chimera can be utilized in high content screens for novel estrogen receptor modulators...
  49. ncbi request reprint High-content fluorescence-based screening for epigenetic modulators
    Elisabeth D Martinez
    Laboratory of Receptor Biology and Gene Expression, Hormone Action and Oncogenesis Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Methods Enzymol 414:21-36. 2006
    ..Furthermore, options for evaluating the mechanism of action of these compounds are presented and principles to govern the choice of hit compounds for the development of leads are discussed...
  50. ncbi request reprint Single-cell analysis of glucocorticoid receptor action reveals that stochastic post-chromatin association mechanisms regulate ligand-specific transcription
    Ty C Voss
    Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Mol Endocrinol 20:2641-55. 2006
    ....
  51. pmc Expanding horizons for nuclear receptors
    Malgorzata Wiench
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    EMBO Rep 11:569-71. 2010
    ..The meeting covered all aspects of regulation by nuclear receptors and provided a good orientation for investigators new to the area, as well as a timely update for those with long tenure in the field...
  52. pmc Differential in vivo binding dynamics of somatic and oocyte-specific linker histones in oocytes and during ES cell nuclear transfer
    Matthias Becker
    National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:3887-95. 2005
    ..These results provide the first mechanistic insights into the crucial step of linker histone replacement as it occurs during fertilization and somatic cell nuclear transfer...
  53. ncbi request reprint 2-methoxyestradiol induces mammary gland differentiation through amphiregulin-epithelial growth factor receptor-mediated signaling: molecular distinctions from the mammary gland of pregnant mice
    Jung im Huh
    Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Building 41, 41 Medlars Drive, Bethesda, MD 20892, USA
    Endocrinology 148:1266-77. 2007
    ..These data demonstrate that 2ME(2) can induce a partial differentiation of the mammary gland through mechanisms that differ from those normally used during pregnancy...
  54. pmc Dynamic behavior of transcription factors on a natural promoter in living cells
    Matthias Becker
    Laboratory of Receptor Biology and Gene Expression, Building 41 Room B602, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 5055, USA
    EMBO Rep 3:1188-94. 2002
    ..Our results indicate that GR and GRIP-1 as components of the activating complex are in a dynamic equilibrium with the promoter, and must return to the template many times during the course of transcriptional activation...
  55. ncbi request reprint Phosphorylation of p53 at serine 37 is important for transcriptional activity and regulation in response to DNA damage
    Kathleen M Dohoney
    Basic Research Laboratory, Virus Tumor Biology Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Oncogene 23:49-57. 2004
    ..Furthermore, in vitro phosphatase assays show that PP2A dephosphorylates p53 at S37. These results suggest that dephosphorylation of p53 at S37 plays a role in the transcriptional regulation of the p53 protein in response to DNA damage...
  56. pmc NSD1 is essential for early post-implantation development and has a catalytically active SET domain
    Geetha Vani Rayasam
    Laboratory of Receptor Biology and Gene Expression, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 22:3153-63. 2003
    ..We have also examined the enzymatic potential of NSD1 and found that its SET domain possesses intrinsic histone methyltransferase activity with specificity for Lys36 of histone H3 (H3-K36) and Lys20 of histone H4 (H4-K20)...
  57. pmc Ligand-specific dynamics of the androgen receptor at its response element in living cells
    Tove I Klokk
    Department of Molecular Biosciences, University of Oslo, Postboks 1041 Blindern, 0316 Oslo, Norway
    Mol Cell Biol 27:1823-43. 2007
    ..These data provide a kinetic and mechanistic basis for regulation of gene expression by androgens and antiandrogens in living cells...
  58. ncbi request reprint Imaging in situ protein-DNA interactions in the cell nucleus using FRET-FLIM
    Frédéric G E Cremazy
    Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
    Exp Cell Res 309:390-6. 2005
    ..The method was successfully applied to the DNA-binding proteins histone H2B and the glucocorticoid receptor and to the heterochromatin-associated proteins HP1alpha and HP1beta...
  59. pmc Formation of higher-order secondary and tertiary chromatin structures by genomic mouse mammary tumor virus promoters
    Philippe T Georgel
    Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 3900, USA
    Genes Dev 17:1617-29. 2003
    ..These results suggest that transcriptionally active eukaryotic promoters may function in a locally folded chromatin environment in vivo...
  60. ncbi request reprint Independent actions on cyclin-dependent kinases and aryl hydrocarbon receptor mediate the antiproliferative effects of indirubins
    Marie Knockaert
    C N R S, Cell Cycle Group and UPS 2682, Station Biologique, BP 74, 29682 Roscoff Cedex, Bretagne, France
    Oncogene 23:4400-12. 2004
    ..In contrast, kinase inhibition, rather than AhR activation, represents the main mechanism underlying the cytotoxic properties of this class of promising antitumor molecules...
  61. ncbi request reprint Molecular cloning and characterization of STAMP2, an androgen-regulated six transmembrane protein that is overexpressed in prostate cancer
    Ceren G Korkmaz
    Department of Molecular Biosciences, University of Oslo, Postboks 1050 Blindern, 0316 Oslo, Norway
    Oncogene 24:4934-45. 2005
    ..Taken together, these data suggest that STAMP2 may contribute to the normal biology of the prostate cell, as well as prostate cancer progression...
  62. ncbi request reprint Molecular cloning and characterization of STAMP1, a highly prostate-specific six transmembrane protein that is overexpressed in prostate cancer
    Kemal S Korkmaz
    Department of Biology and the Biotechnology Centre of Oslo, University of Oslo, Postboks 1050 Blindern, 0316 Oslo, Norway
    J Biol Chem 277:36689-96. 2002
    ..Taken together, these data suggest that STAMP1 may have an important role in the normal prostate cell as well as in prostate cancer progression...
  63. pmc The ligand binding domain controls glucocorticoid receptor dynamics independent of ligand release
    Sebastiaan H Meijsing
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, 600 16th Street, Room GH S574, San Francisco, CA 94107 2280, USA
    Mol Cell Biol 27:2442-51. 2007
    ..Overall, these studies showed that activities impinging on the LBD regulate GR exchange with GREs but that the dissociation of GR from GREs is independent from ligand dissociation...
  64. pmc Glutaraldehyde modified mica: a new surface for atomic force microscopy of chromatin
    Hongda Wang
    Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287, USA
    Biophys J 83:3619-25. 2002
    ..We foresee many important applications for this surface in future atomic force microscopy studies of chromatin...
  65. ncbi request reprint Glucocorticoid receptor domain requirements for chromatin remodeling and transcriptional activation of the mouse mammary tumor virus promoter in different nucleoprotein contexts
    Erika Krasnickas Keeton
    Department of Genetics, George Washington University, Washington, DC, USA
    J Biol Chem 277:28247-55. 2002
    ..Within a particular cellular environment the GR appears to possess a significant degree of versatility in the mechanism by which it activates a target promoter...
  66. pmc Actin-dependent intranuclear repositioning of an active gene locus in vivo
    Miroslav Dundr
    Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA
    J Cell Biol 179:1095-103. 2007
    ..In the presence of a dominant-negative mutant of beta-actin, the repositioning of activated U2 genes is markedly inhibited. This supports a model in which nuclear actin is required for these rapid, long-range chromosomal movements...