Philip A Gurnev

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint Inversion of membrane surface charge by trivalent cations probed with a cation-selective channel
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, United States
    Langmuir 28:15824-30. 2012
  2. pmc Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers
    Philip A Gurnev
    Program in Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    FEBS Lett 586:2529-34. 2012
  3. ncbi request reprint Docking of a single phage lambda to its membrane receptor maltoporin as a time-resolved event
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 359:1447-55. 2006
  4. pmc Osmotic stress regulates the strength and kinetics of sugar binding to the maltoporin channel
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    J Phys Condens Matter 22:454110. 2010
  5. pmc Probing tubulin-blocked state of VDAC by varying membrane surface charge
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Biophys J 102:2070-6. 2012
  6. pmc Membrane lipid composition regulates tubulin interaction with mitochondrial voltage-dependent anion channel
    Tatiana K Rostovtseva
    Program in Physical Biology, Eunice Kennedy Shriver NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 287:29589-98. 2012
  7. pmc Tubulin-blocked state of VDAC studied by polymer and ATP partitioning
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS Lett 585:2363-6. 2011
  8. pmc Alpha-Synuclein Lipid-Dependent Membrane Binding and Translocation through the α-Hemolysin Channel
    Philip A Gurnev
    Physics Department, University of Massachusetts, Amherst, Massachusetts Section on Molecular Transport, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland Electronic address
    Biophys J 106:556-65. 2014
  9. pmc The dynamic side of the Hofmeister effect: a single-molecule nanopore study of specific complex formation
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, 9000 Rockville Pike, Bldg 9, Bethesda, MD 20892, USA
    Chemphyschem 10:1445-9. 2009
  10. pmc Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compounds
    Godfrey Lisk
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    Biochim Biophys Acta 1798:1679-88. 2010

Collaborators

Detail Information

Publications12

  1. doi request reprint Inversion of membrane surface charge by trivalent cations probed with a cation-selective channel
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, United States
    Langmuir 28:15824-30. 2012
    ..Specifically, these concentrations are close to 0.05 mM for lanthanum, 0.25 mM for hexaamminecobalt, and 4 mM for spermidine...
  2. pmc Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers
    Philip A Gurnev
    Program in Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    FEBS Lett 586:2529-34. 2012
    ..These findings allow us to suggest that TisB forms clusters of narrow pores that are essential for its mechanism of action...
  3. ncbi request reprint Docking of a single phage lambda to its membrane receptor maltoporin as a time-resolved event
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 359:1447-55. 2006
    ..We expect that the present study contributes to the current needs for structural information on the functional complexes involved in intercellular recognition...
  4. pmc Osmotic stress regulates the strength and kinetics of sugar binding to the maltoporin channel
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    J Phys Condens Matter 22:454110. 2010
    ..As a practical outcome, our results also demonstrate how osmotic stress can improve single-molecule detection of different solutes using protein-based nanopores...
  5. pmc Probing tubulin-blocked state of VDAC by varying membrane surface charge
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Biophys J 102:2070-6. 2012
    ....
  6. pmc Membrane lipid composition regulates tubulin interaction with mitochondrial voltage-dependent anion channel
    Tatiana K Rostovtseva
    Program in Physical Biology, Eunice Kennedy Shriver NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 287:29589-98. 2012
    ..More generally, our findings give an example of the lipid-controlled protein-protein interaction where the choice of lipid species is able to change the equilibrium binding constant by orders of magnitude...
  7. pmc Tubulin-blocked state of VDAC studied by polymer and ATP partitioning
    Philip A Gurnev
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS Lett 585:2363-6. 2011
    ..34±0.15, the selectivity reverses from initially anionic to cationic. Directly measuring ATP partitioning we demonstrate that these changes prohibit ATP from entering the channel in its tubulin-blocked state...
  8. pmc Alpha-Synuclein Lipid-Dependent Membrane Binding and Translocation through the α-Hemolysin Channel
    Philip A Gurnev
    Physics Department, University of Massachusetts, Amherst, Massachusetts Section on Molecular Transport, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland Electronic address
    Biophys J 106:556-65. 2014
    ..Taken together, these results demonstrate that β-barrel channels may serve as sensitive probes of α-syn interactions with membranes as well as model systems for studies of channel-assisted protein transport. ..
  9. pmc The dynamic side of the Hofmeister effect: a single-molecule nanopore study of specific complex formation
    Philip A Gurnev
    Laboratory of Physical and Structural Biology, Program in Physical Biology, NICHD, National Institutes of Health, 9000 Rockville Pike, Bldg 9, Bethesda, MD 20892, USA
    Chemphyschem 10:1445-9. 2009
    ..The kinetics underlying the dynamic equilibrium are studied to reveal qualitatively different dynamic actions of various cosolute salts...
  10. pmc Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compounds
    Godfrey Lisk
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    Biochim Biophys Acta 1798:1679-88. 2010
    ..These findings are consistent with a shared channel that mediates uptake of ions, nutrients and toxins. This channel's gating and selectivity properties can be modified in response to in vitro selective pressure...
  11. pmc Cationic cell-penetrating peptide binds to planar lipid bilayers containing negatively charged lipids but does not induce conductive pores
    Philip A Gurnev
    Department of Physics, University of Massachusetts, Amherst, Massachusetts, USA
    Biophys J 104:1933-9. 2013
    ....
  12. ncbi request reprint Functional subconformations in protein folding: evidence from single-channel experiments
    Lisen Kullman
    Laboratory of Physical and Structural Biology, NICHD, NIH, Bethesda, Maryland 20892 0924, USA
    Phys Rev Lett 96:038101. 2006
    ..We show that the disorder responsible for variations in ion conductance does not affect sugar binding, suggesting that Maltoporin can exist in a wide set of fully functional, yet distinctly different, subconformations...