Shiv Is Grewal

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Heterochromatin and epigenetic control of gene expression
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 301:798-802. 2003
  2. pmc RNAi-dependent formation of heterochromatin and its diverse functions
    Shiv Is Grewal
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Genet Dev 20:134-41. 2010
  3. pmc Transcription and RNA interference in the formation of heterochromatin
    Shiv I S Grewal
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 447:399-406. 2007
  4. ncbi request reprint Heterochromatin revisited
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Genet 8:35-46. 2007
  5. ncbi request reprint Regulation of heterochromatin by histone methylation and small RNAs
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Opin Cell Biol 16:230-8. 2004
  6. doi request reprint Cell cycle control of centromeric repeat transcription and heterochromatin assembly
    Ee Sin Chen
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 451:734-7. 2008
  7. ncbi request reprint RNAi-independent heterochromatin nucleation by the stress-activated ATF/CREB family proteins
    Songtao Jia
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 304:1971-6. 2004
  8. ncbi request reprint RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing
    Ken ichi Noma
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 36:1174-80. 2004
  9. ncbi request reprint The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast
    Takatomi Yamada
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 20:173-85. 2005
  10. ncbi request reprint Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats
    Martin Zofall
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 22:681-92. 2006

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Heterochromatin and epigenetic control of gene expression
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 301:798-802. 2003
    ..Finally, an unexpected role for noncoding RNAs and RNA interference in the formation of epigenetic chromatin domains has been uncovered...
  2. pmc RNAi-dependent formation of heterochromatin and its diverse functions
    Shiv Is Grewal
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Genet Dev 20:134-41. 2010
    ..Recently, heterochromatin factors have been shown to suppress antisense RNAs at euchromatic loci. These findings define conserved pathways that probably have major impact on the epigenetic regulation of eukaryotic genomes...
  3. pmc Transcription and RNA interference in the formation of heterochromatin
    Shiv I S Grewal
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 447:399-406. 2007
    ..Silencing of chromatin might involve trans-acting sources of the crucial small RNAs that carry out RNA interference, but in some cases, transcription of the region to be silenced seems to be required--an apparent contradiction...
  4. ncbi request reprint Heterochromatin revisited
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Genet 8:35-46. 2007
    ....
  5. ncbi request reprint Regulation of heterochromatin by histone methylation and small RNAs
    Shiv I S Grewal
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Opin Cell Biol 16:230-8. 2004
    ..These new findings define a novel conserved mechanism of heterochromatin formation that is likely to have an impact on all eukaryotic silencing pathways...
  6. doi request reprint Cell cycle control of centromeric repeat transcription and heterochromatin assembly
    Ee Sin Chen
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 451:734-7. 2008
    ..Together, these processes probably facilitate heterochromatin maintenance through successive cell divisions...
  7. ncbi request reprint RNAi-independent heterochromatin nucleation by the stress-activated ATF/CREB family proteins
    Songtao Jia
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 304:1971-6. 2004
    ..These analyses link ATF/CREB family proteins, involved in cellular response to environmental stresses, to nucleation of constitutive heterochromatin...
  8. ncbi request reprint RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing
    Ken ichi Noma
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 36:1174-80. 2004
    ..This tethering promotes the processing of transcripts and generation of additional siRNAs for heterochromatin maintenance...
  9. ncbi request reprint The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast
    Takatomi Yamada
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 20:173-85. 2005
    ....
  10. ncbi request reprint Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats
    Martin Zofall
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 22:681-92. 2006
    ..Our analyses suggest that Swi6/HP1 recruits opposing chromatin-modifying activities, the balancing of which is crucial for heterochromatin maintenance...
  11. doi request reprint Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin
    Ke Zhang
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 15:381-8. 2008
    ..Our analyses delineate sequential steps for the assembly of heterochromatic domains and suggest that the ability of Clr4 to both 'write' and 'read' H3K9me facilitates heterochromatin maintenance through successive cell divisions...
  12. ncbi request reprint HULC, a histone H2B ubiquitinating complex, modulates heterochromatin independent of histone methylation in fission yeast
    Martin Zofall
    Laboratory of Biochemistry and Molecular Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 282:14065-72. 2007
    ..These analyses implicate ubH2B in modulation of heterochromatin, which has important implications for dynamics and many functions associated with heterochromatic structures...
  13. ncbi request reprint Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome
    Hugh P Cam
    Laboratory of Molecular Cell Biology, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Nat Genet 37:809-19. 2005
    ..Our analyses also uncover an important role for the RNAi machinery in maintaining genomic integrity...
  14. pmc RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production
    Tomoyasu Sugiyama
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    Proc Natl Acad Sci U S A 102:152-7. 2005
    ..These results not only implicate Rdp1 as an essential component of a self-enforcing RNAi loop but also ascribe a critical role for its RNA-dependent RNA polymerase activity in siRNA production necessary for heterochromatin formation...
  15. ncbi request reprint Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination
    Songtao Jia
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 119:469-80. 2004
    ..Our analyses implicate heterochromatin in long-range regulatory interactions and suggest that heterochromatin imposes at the mating-type region structural organization that is important for the donor-choice mechanism...
  16. pmc Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs
    Martin Zofall
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health Bethesda, Maryland 20892, USA
    Nature 461:419-22. 2009
    ..These analyses suggest that as well as performing other functions, H2A.Z is a component of a genome indexing mechanism that cooperates with heterochromatin and RNAi factors to suppress read-through antisense transcripts...
  17. pmc Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast
    Tamas Fischer
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:8998-9003. 2009
    ....
  18. ncbi request reprint Fission yeast homologs of human histone H3 lysine 4 demethylase regulate a common set of genes with diverse functions
    Estelle Nicolas
    Laboratory of Molecular Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:35983-8. 2006
    ..Importantly, we show that SWIRM1 is not only required for optimum transcription of its target genes but also display a global role in regulation of antisense transcription...
  19. ncbi request reprint Host genome surveillance for retrotransposons by transposon-derived proteins
    Hugh P Cam
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    Nature 451:431-6. 2008
    ....
  20. ncbi request reprint SHREC, an effector complex for heterochromatic transcriptional silencing
    Tomoyasu Sugiyama
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 128:491-504. 2007
    ..Our analyses suggest that SHREC regulates nucleosome positioning to assemble higher-order chromatin structures critical for heterochromatin functions...
  21. doi request reprint Transcriptional scaffolds for heterochromatin assembly
    Hugh P Cam
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 136:610-4. 2009
    ....
  22. ncbi request reprint Distinct roles of HDAC complexes in promoter silencing, antisense suppression and DNA damage protection
    Estelle Nicolas
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, US National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 14:372-80. 2007
    ....
  23. ncbi request reprint A role for TFIIIC transcription factor complex in genome organization
    Ken ichi Noma
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 125:859-72. 2006
    ..Our analyses uncover a general genome organization mechanism involving conserved TFIIIC complex...
  24. ncbi request reprint Ubiquitin ligase component Cul4 associates with Clr4 histone methyltransferase to assemble heterochromatin
    Songtao Jia
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 7:1007-13. 2005
    ..These analyses uncover a role for Cul4-based protein ubiquitination in regulating H3K9me and heterochromatin formation...
  25. pmc Different means, same end-heterochromatin formation by RNAi and RNAi-independent RNA processing factors in fission yeast
    Francisca E Reyes-Turcu
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Genet Dev 22:156-63. 2012
    ..These findings have established that, in addition to coding for proteins, mRNAs also function as signaling molecules that modify chromatin structure by targeting heterochromatin assembly factors...
  26. pmc Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators
    Young Eun Leem
    Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 30:98-107. 2008
    ..Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration...
  27. pmc Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe
    Stephanie A Morris
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Eukaryot Cell 4:1446-54. 2005
    ..These results, along with our finding that K36 methylation is highly conserved among eukaryotes, imply a conserved role for this modification in the transcription elongation process...
  28. ncbi request reprint Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast
    Nobuhiro Nonaka
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Nat Cell Biol 4:89-93. 2002
    ..These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes...
  29. ncbi request reprint Heterochromatin: new possibilities for the inheritance of structure
    Shiv I S Grewal
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Curr Opin Genet Dev 12:178-87. 2002
    ....
  30. pmc Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications
    Hiromi Nakagawa
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Genes Dev 16:1766-78. 2002
    ..These results indicate that the CENP-B homologs act as site-specific nucleation factors for the formation of centromeric heterochromatin by heterochromatin-specific modifications of histone tails...
  31. ncbi request reprint Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    Thomas A Volpe
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
    Science 297:1833-7. 2002
    ..We propose that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi...
  32. ncbi request reprint Establishment and maintenance of a heterochromatin domain
    Ira M Hall
    Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA
    Science 297:2232-7. 2002
    ..This work defines sequential requirements for the initiation and propagation of regional heterochromatic domains...
  33. pmc Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast
    Ken ichi Noma
    Cold Spring Harbor Laboratory, P O Box 100, NY 11724, USA
    Proc Natl Acad Sci U S A 99:16438-45. 2002
    ....
  34. ncbi request reprint Structure of the SET domain histone lysine methyltransferase Clr4
    Jinrong Min
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Nat Struct Biol 9:828-32. 2002
    ..The structure provides insights into the architecture of SET domain histone methyltransferases and establishes a paradigm for further characterization of the Clr4 family of epigenetic regulators...
  35. pmc Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity
    Jun Ichi Nakayama
    Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, NY 11724, USA
    EMBO J 22:2776-87. 2003
    ..These results link the MRG family protein Alp13 to histone deacetylation, and suggest that Clr6 and its associated factors are essential for fundamental chromosomal events...
  36. pmc A novel jmjC domain protein modulates heterochromatization in fission yeast
    Nabieh Ayoub
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, Jerusalem, Israel 91010
    Mol Cell Biol 23:4356-70. 2003
    ..Furthermore, overexpression of Epe1 elevates the rate of chromosome loss. We propose that Epe1 helps control chromatin organization by down-regulating the stability of epigenetic marks that govern heterochromatization...
  37. ncbi request reprint Sir2 regulates histone H3 lysine 9 methylation and heterochromatin assembly in fission yeast
    Gurumurthy D Shankaranarayana
    Cold Spring Harbor Laboratory, P O Box 100, Cold Spring Harbor, NY 11724, USA
    Curr Biol 13:1240-6. 2003
    ..Our findings suggest that the NAD(+)-dependent deacetylase Sir2 plays an important and conserved role in heterochromatin assembly in eukaryotes...
  38. ncbi request reprint Heterochromatin: silence is golden
    Sarah C R Elgin
    Department of Biology, CB 1229, Washington University, One Brookings Drive, St Louis, MO 63130, USA
    Curr Biol 13:R895-8. 2003
  39. pmc RNAi-mediated targeting of heterochromatin by the RITS complex
    Andre Verdel
    Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA
    Science 303:672-6. 2004
    ..The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci...
  40. pmc Biochemical interactions between proteins and mat1 cis-acting sequences required for imprinting in fission yeast
    Bum Soo Lee
    Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Mol Cell Biol 24:9813-22. 2004
    ..These results suggest that the stimulatory effect of swi1 and swi3 on switching and imprinting occurs through interaction of the Swi1p-Swi3p complex with the mat1 regions...
  41. pmc A discrete class of intergenic DNA dictates meiotic DNA break hotspots in fission yeast
    Gareth A Cromie
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS Genet 3:e141. 2007
    ....
  42. pmc RNA interference machinery regulates chromosome dynamics during mitosis and meiosis in fission yeast
    Ira M Hall
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, P O Box 100, NY 11724, USA
    Proc Natl Acad Sci U S A 100:193-8. 2003
    ..This study demonstrates that the fission yeast RNAi machinery is required for the proper regulation of chromosome architecture during mitosis and meiosis...