Research Topics
Species | M T GladwinSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeuticsDaniel B Kim-Shapiro
Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
Arterioscler Thromb Vasc Biol 26:697-705. 2006..As subsequently understood, the hypoxic generation of NO from nitrite is likely to prove important in many aspects of physiology, pathophysiology, and therapeutics...- Low-dose intravenous nitrite improves hemodynamics in a canine model of acute pulmonary thromboembolismCarlos A C Dias-Junior
Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto, SP, Brazil
Free Radic Biol Med 41:1764-70. 2006..These findings suggest a new therapeutic application for nitrite and support emerging evidence for a surprisingly potent and potentially physiological vasoactivity of nitrite... 
Hemodynamic and functional assessment of patients with sickle cell disease and pulmonary hypertensionAnastasia Anthi
Vascular Medicine Branch, Clinical Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 2089, USA
Am J Respir Crit Care Med 175:1272-9. 2007..Although pulmonary hypertension (PH) is a common complication of sickle cell disease (SCD) associated with high mortality, there exist few data characterizing hemodynamics and cardiopulmonary function in this population...- Inhaled nitric oxide augments nitric oxide transport on sickle cell hemoglobin without affecting oxygen affinityM T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
J Clin Invest 104:937-45. 1999..The levels of nitrosylated hemoglobin are too low to affect overall hemoglobin oxygen affinity, but augmented NO transport to the microvasculature seems a promising strategy for improving microvascular perfusion... - Divergent nitric oxide bioavailability in men and women with sickle cell diseaseMark T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
Circulation 107:271-8. 2003..Although reduced endothelial nitric oxide (NO) bioavailability has been demonstrated in arteriosclerotic vascular disease, the integrity of this system in sickle cell disease remains uncertain... - Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"Mark T Gladwin
Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 41:557-61. 2006 - Nitric oxide's reactions with hemoglobin: a view through the SNO-stormMark T Gladwin
Critical Care Medicine Department of the Warren G. Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
Nat Med 9:496-500. 2003 - The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulationMark T Gladwin
Critical Care Medicine Department, Clinical Center, Laboratory of Chemical Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
Free Radic Biol Med 36:707-17. 2004..This newly described role of hemoglobin as a nitrite reductase is discussed in the context of blood flow regulation, oxygen sensing, and nitrite-based therapeutics... - Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndromeMark T Gladwin
Vascular Medicine Branch, National Heart, Lung, and Blood Institute, and Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
J Clin Invest 116:2330-2. 2006..Diseases associated with NO resistance would appear to be ideally suited for therapies directed at restoring redox homeostasis, sGC activity, and NO sensitivity... - The emerging biology of the nitrite anionMark T Gladwin
Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
Nat Chem Biol 1:308-14. 2005..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics... - Haldane, hot dogs, halitosis, and hypoxic vasodilation: the emerging biology of the nitrite anionMark T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
J Clin Invest 113:19-21. 2004..Nitrite levels in blood reflect NO production from endothelial NO synthase enzymes, and recent data suggest that nitrite contributes to blood flow regulation by reaction with deoxygenated hemoglobin and tissue heme proteins to form NO... - Pulmonary hypertension as a risk factor for death in patients with sickle cell diseaseMark T Gladwin
Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
N Engl J Med 350:886-95. 2004..The prevalence of pulmonary hypertension in adults with sickle cell disease, the mechanism of its development, and its prospective prognostic significance are unknown... - Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilationMark T Gladwin
Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
Am J Physiol Heart Circ Physiol 291:H2026-35. 2006..An expanded consideration of nitrite as a hypoxia-dependent intrinsic signaling molecule has opened up a new field of research and therapeutic opportunities for diseases associated with regional hypoxia and vasoconstriction... - The functional nitrite reductase activity of the heme-globinsMark T Gladwin
Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
Blood 112:2636-47. 2008..We will review these reactions in the biologic framework of hypoxic signaling in blood and the heart... - S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric functionMark T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
J Biol Chem 277:27818-28. 2002.... - Relative role of heme nitrosylation and beta-cysteine 93 nitrosation in the transport and metabolism of nitric oxide by hemoglobin in the human circulationM T Gladwin
Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 97:9943-8. 2000.... - Nitric oxide transport on sickle cell hemoglobin: where does it bind?M T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Res 35:175-80. 2001.... - Retinoic acid reduces p11 protein levels in bronchial epithelial cells by a posttranslational mechanismM T Gladwin
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
Am J Physiol Lung Cell Mol Physiol 279:L1103-9. 2000..006). Therefore, RA reduces p11 protein expression and increases PLA(2) activity and AA release... - Nitric oxide donor properties of hydroxyurea in patients with sickle cell diseaseMark T Gladwin
Critical Care Medicine Department of the Warren G Magnuson Clinical Center, Bethesda, MD 20892 1662, USA
Br J Haematol 116:436-44. 2002..The role of NO in the induction of fetal haemoglobin and possible synergy between NO donor therapy and classic cytostatic and differentiating medications should be explored... - Effects of inhaled nitric oxide on regional blood flow are consistent with intravascular nitric oxide deliveryR O Cannon
Cardiology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892 1650, USA
J Clin Invest 108:279-87. 2001..This effect may have application for the treatment of diseases characterized by endothelial dysfunction... - Role of circulating nitrite and S-nitrosohemoglobin in the regulation of regional blood flow in humansM T Gladwin
Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 97:11482-7. 2000.... - Noninvasive determination of spatially resolved and time-resolved tissue perfusion in humans during nitric oxide inhibition and inhalation by use of a visible-reflectance hyperspectral imaging techniqueK J Zuzak
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda MD 20892-0510, USA
Circulation 104:2905-10. 2001..This imaging method may provide an effective approach for time-resolved noninvasive monitoring of skin hemoglobin oxygen saturation and assessment of responses to therapeutic interventions in patients with vascular disease... - Nitric oxide therapy in sickle cell diseaseM T Gladwin
Critical Care Medicine Department, Warren G. Magnuson Clinical Center, NIH, Bethesda, MD 20892-1662, USA
Semin Hematol 38:333-42. 2001..While direct evidence for a clinical benefit of NO therapy in sickle cell disease has not been reported, studies are underway to determine if inhaled NO will reduce the substantial morbidity and mortality suffered by these patients... - Regional cerebral hyperperfusion and nitric oxide pathway dysregulation in Fabry disease: reversal by enzyme replacement therapyD F Moore
Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1260, USA
Circulation 104:1506-12. 2001..CONCLUSIONS: These findings suggest a chronic alteration of the nitric oxide pathway in Fabry disease, with critical protein nitration that is reversible with enzyme replacement therapy... - Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failureM K Holly
Renal Diagnostics and Therapeutics Unit, NIDDK, Bethesda, Maryland, USA
Kidney Int 70:496-506. 2006..This model allowed us to use DIGE to find changes in urinary proteins and this approach identified a potential biomarker and drug target - meprin-1-alpha... - Disorders of ciliary motilityM J Cowan
Department of Critical Care Medicine, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
Am J Med Sci 321:3-10. 2001..Late stages of the disease may require surgical intervention for bronchiectasis or lung transplant for end-stage lung disease... - Endothelial progenitor cell mobilization and increased intravascular nitric oxide in patients undergoing cardiac rehabilitationJonathan D Paul
Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
J Cardiopulm Rehabil Prev 27:65-73. 2007.... - Severe pulmonary hypertension in an adolescent with sickle cell diseaseJames G Taylor
Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-1476, USA
Am J Hematol 83:71-2. 2008 - Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobinRozalina Grubina
Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 282:12916-27. 2007.... - Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypesGregory J Kato
Vascular Medicine Branch, National Heart, Lung and Blood Institute, Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Drive, Building 10CRC 5 5140, Bethesda, MD 20892 1476, USA
Blood Rev 21:37-47. 2007..Some of these drugs are now being studied in clinical trials... - Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension, and nitric oxide scavenging by cell-free hemoglobinJose Villagra
Vascular Medicine Branch of National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1476, USA
Blood 110:2166-72. 2007..This supports a role for NO-based therapeutics for SCD vasculopathy. This trial was registered at www.clinicaltrials.gov as no. NCT00352430... - Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasisSruti Shiva
Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Chem Biol 2:486-93. 2006.... - Measurement of nitric oxide levels in the red cell: validation of tri-iodide-based chemiluminescence with acid-sulfanilamide pretreatmentXunde Wang
Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 281:26994-7002. 2006.... - Hemoglobin oxygen fractional saturation regulates nitrite-dependent vasodilation of aortic ring bioassaysT Scott Isbell
Department of Pathology, and Center for Free Radical Biology, University of Alabama at Birmingham, Alabama 35296, USA
Am J Physiol Heart Circ Physiol 293:H2565-72. 2007.... - Nitrite infusion in humans and nonhuman primates: endocrine effects, pharmacokinetics, and tolerance formationAndre Dejam
Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Circulation 116:1821-31. 2007..However, important questions of potency, kinetics, mechanism of action, and possible induction of tolerance remain unanswered... - Nitrite reductase activity of hemoglobin as a systemic nitric oxide generator mechanism to detoxify plasma hemoglobin produced during hemolysisPeter C Minneci
Department of Surgery, The Children s Institute for Surgical Science, The Children s Hospital of Philadelphia, PA, USA
Am J Physiol Heart Circ Physiol 295:H743-54. 2008..Nitrite infusions may promote NO generation from Hb while maintaining oxygen delivery; this effect could be harnessed to treat hemolytic conditions and to detoxify Hb-based blood substitutes... - Nitrite anion provides potent cytoprotective and antiapoptotic effects as adjunctive therapy to reperfusion for acute myocardial infarctionFelix M Gonzalez
Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1061, USA
Circulation 117:2986-94. 2008.... - The proverbial chicken or the egg? Dissection of the role of cell-free hemoglobin versus reactive oxygen species in sickle cell pathophysiologyMegan L Krajewski
Am J Physiol Heart Circ Physiol 295:H4-7. 2008 - Prevalence and risk factors for pulmonary artery systolic hypertension among sickle cell disease patients in NigeriaZakari Y Aliyu
Department of Medicine and Center for Sickle Cell Disease, Howard University, Washington, District of Columbia 20060, USA
Am J Hematol 83:485-90. 2008..The inclusion of African sites in sickle cell pulmonary hypertension clinical trials should be encouraged... 
Sickle cell disease vasculopathy: a state of nitric oxide resistanceKatherine C Wood
Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 44:1506-28. 2008..New therapeutic strategies to limit intravascular hemolysis and ROS generation and increase NO bioavailability are discussed...- Chronic hyper-hemolysis in sickle cell anemia: association of vascular complications and mortality with less frequent vasoocclusive painJames G Taylor
Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 3:e2095. 2008..We have previously reported that intense hemolysis is associated with increased risk of vascular complications in a small cohort of adults with sickle cell disease. These observations have not been validated in other populations... - The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobinXiaojun He
Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
Free Radic Biol Med 44:1420-32. 2008..These results support the notion that Angeli's salt or a similar compound could be used to effectively treat conditions associated with intravascular hemolysis... - Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteinsXunde Wang
Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 44:1362-72. 2008..The addition of copper to ascorbate allows for a simple assay modification that dramatically increases sensitivity while maintaining specificity... - The nitrate-nitrite-nitric oxide pathway in physiology and therapeuticsJon O Lundberg
Department of Physiology and Pharmacology, Karolinska Institute, SE 171 77 Stockholm, Sweden
Nat Rev Drug Discov 7:156-67. 2008.... - Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinityRozalina Grubina
Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 283:3628-38. 2008..From a pathophysiological standpoint, under conditions where HbS polymers form, the rate of nitrite reduction is reduced compared with HbA and solution-phase HbS, indicating that HbS polymers reduce nitrite more slowly... - Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobinSwati Basu
Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
Nat Chem Biol 3:785-94. 2007.... - Sickle cell disease and pulmonary hypertension in Africa: a global perspective and review of epidemiology, pathophysiology, and managementZakari Y Aliyu
Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
Am J Hematol 83:63-70. 2008..There is clearly a need to include Africa and other parts of the world with high SCD prevalence in future comprehensive studies on the epidemiology and treatment of end organ complications of an aging SCD population world-wide... - Pulmonary vascular effects of red blood cells containing S-nitrosated hemoglobinSteven Deem
Dept of Anesthesiology, PO Box 359724, Harborview Medical Center, Seattle, WA 98104 2499, USA
Am J Physiol Heart Circ Physiol 287:H2561-8. 2004.... - Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilatorChristian J Hunter
Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892-1662, USA
Nat Med 10:1122-7. 2004.... - How do red blood cells dilate blood vessels?--ReplyDaniel B Kim-Shapiro
Circ Res 95:e10. 2004 - The perfusion paradox and vascular instability in sickle cell diseaseKarl A Nath
Division of Nephrology, Mayo Clinic, Rochester, Minnesota 55905, USA
Microcirculation 11:179-93. 2004..Finally, the possibility that the induction of heme oxygenase-1, recently described in SCD, may confer a protective response in the vasculature and other tissues is discussed... - Biological activity of nitric oxide in the plasmatic compartmentXunde Wang
Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-1662, USA
Proc Natl Acad Sci U S A 101:11477-82. 2004..The studies further suggest that alternative NO reaction end products in plasma, such as nitrite, N-nitrosamines, iron-nitrosyls, and nitrated lipids, should be evaluated in blood NO transport along the vasculature... - NO contest: nitrite versus S-nitroso-hemoglobinMark T Gladwin
Circ Res 94:851-5. 2004 - Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulationKenyatta Cosby
Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
Nat Med 9:1498-505. 2003..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation... - Hemolytic anemia-associated pulmonary hypertension of sickle cell disease and the nitric oxide/arginine pathwayMaria L Jison
Am J Respir Crit Care Med 168:3-4. 2003 - Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxideKarel J Zuzak
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases/NIH, 9000 Rockville Pike, Bethesda, MD 20892-0510, USA
Am J Physiol Heart Circ Physiol 285:H1183-9. 2003.... - Methodologies for the sensitive and specific measurement of S-nitrosothiols, iron-nitrosyls, and nitrite in biological samplesBenjamin K Yang
Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892-1662, USA
Free Radic Res 37:1-10. 2003 - An emerging role for nitric oxide in sickle cell disease vascular homeostasis and therapyChristopher D Reiter
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1662, USA
Curr Opin Hematol 10:99-107. 2003..Possible effects of chronic nitric oxide-based therapies on erythrocyte density, pulmonary artery pressures, and fetal hemoglobin induction deserve study... - Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell diseaseChristopher D Reiter
Critical Care Medicine Department of the Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
Nat Med 8:1383-9. 2002..Decompartmentalization of hemoglobin and subsequent dioxygenation of nitric oxide may explain the vascular complications shared by acute and chronic hemolytic disorders... - Nitric oxide (NO) in normal and hypoxic vascular regulation of the spiny dogfish, Squalus acanthiasKai E Swenson
Mt Desert Island Biological Laboratory, Salsbury Cove, Maine 04672, USA
J Exp Zool A Comp Exp Biol 303:154-60. 2005..In acute hypoxia, there is a rapid up-regulation of vascular NO production that appears to be responsible for hypoxic vasodilation... - Hemolytic anemia-associated pulmonary hypertension in sickle cell diseaseElaina E Lin
National Institutes of Health, 31 Center Drive, Bethesda, MD 20892, USA
Curr Hematol Rep 4:117-25. 2005..Finally, we review suggested therapies including the established treatments and new pulmonary vasodilator and remodeling agents in the management of pulmonary hypertension in hemolytic anemias... - Cerebrovascular disease associated with sickle cell pulmonary hypertensionGregory J Kato
Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1476, USA
Am J Hematol 81:503-10. 2006..Clinicians should suspect occult cerebrovascular disease in sickle cell patients with pulmonary hypertension... - Lopinavir-ritonavir: effects on endothelial cell function in healthy subjectsJessica R Grubb
Critical Care Medical Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
J Infect Dis 193:1516-9. 2006..Thus, in this small cohort of subjects, short-term treatment with lopinavir-ritonavir does not appear to directly promote endothelial cell dysfunction... - Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobinPeter C Minneci
Critical Care Medicine Department, NIH, Bethesda, Maryland 20892, USA
J Clin Invest 115:3409-17. 2005.... - Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilationJack H Crawford
Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, 35294, USA
Blood 107:566-74. 2006..These data support a function for RBC hemoglobin as an allosterically and redox-regulated nitrite reductase whose "enzyme activity" couples hypoxia to increased NO-dependent blood flow... - Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenationIvan Azarov
Department of Physics, School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27109, USA
J Biol Chem 280:39024-32. 2005..These observations may have important physiological implications within the microvasculature and for pathophysiological disruption of nitric oxide homeostasis in diseases... - Sildenafil therapy in patients with sickle cell disease and pulmonary hypertensionRoberto F Machado
Vascular Therapeutics Section, Cardiovascular Branch, National Heart Lung and Blood Institute, Bethesda, MD, USA
Br J Haematol 130:445-53. 2005.... - Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric controlZhi Huang
Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892-1662, USA
J Clin Invest 115:2099-107. 2005..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control... - Dysregulated arginine metabolism, hemolysis-associated pulmonary hypertension, and mortality in sickle cell diseaseClaudia R Morris
Department of Emergency Medicine, Children s Hospital and Research Center at Oakland, CA 94609, USA
JAMA 294:81-90. 2005..We hypothesized that increased arginase activity and dysregulated arginine metabolism contribute to endothelial dysfunction, pulmonary hypertension, and patient outcomes... - Hemoglobin as a nitrite reductase regulating red cell-dependent hypoxic vasodilationMark T Gladwin
Am J Respir Cell Mol Biol 32:363-6. 2005 - Erythrocytes are the major intravascular storage sites of nitrite in human bloodAndre Dejam
Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892, USA
Blood 106:734-9. 2005..These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit... - Effects of S-nitrosation and cross-linking of hemoglobin on hypoxic pulmonary vasoconstriction in isolated rat lungsSteven Deem
Department of Anesthesiology, University of Washington and the Veterans Affairs Puget Sound Health Care Center, Seattle, Wash, USA
Circ Res 91:626-32. 2002..However, release of NO from SNO-Hb was not influenced by deoxygenation-mediated allosteric changes in Hb across a broad range of oxyHb saturation. Cross-linking of Hb does not limit its pulmonary vasoconstrictor effects...