M T Gladwin

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Arterioscler Thromb Vasc Biol 26:697-705. 2006
  2. ncbi request reprint Low-dose intravenous nitrite improves hemodynamics in a canine model of acute pulmonary thromboembolism
    Carlos A C Dias-Junior
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av Bandeirantes, 3900, 14049 900, Ribeirao Preto, SP, Brazil
    Free Radic Biol Med 41:1764-70. 2006
  3. pmc Hemodynamic and functional assessment of patients with sickle cell disease and pulmonary hypertension
    Anastasia Anthi
    Vascular Medicine Branch, Clinical Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 2089, USA
    Am J Respir Crit Care Med 175:1272-9. 2007
  4. pmc Inhaled nitric oxide augments nitric oxide transport on sickle cell hemoglobin without affecting oxygen affinity
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 104:937-45. 1999
  5. ncbi request reprint Divergent nitric oxide bioavailability in men and women with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circulation 107:271-8. 2003
  6. ncbi request reprint Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:557-61. 2006
  7. ncbi request reprint Nitric oxide's reactions with hemoglobin: a view through the SNO-storm
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 9:496-500. 2003
  8. ncbi request reprint The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, Laboratory of Chemical Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Free Radic Biol Med 36:707-17. 2004
  9. pmc Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndrome
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, and Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 116:2330-2. 2006
  10. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005

Detail Information

Publications72

  1. ncbi request reprint Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Arterioscler Thromb Vasc Biol 26:697-705. 2006
    ..As subsequently understood, the hypoxic generation of NO from nitrite is likely to prove important in many aspects of physiology, pathophysiology, and therapeutics...
  2. ncbi request reprint Low-dose intravenous nitrite improves hemodynamics in a canine model of acute pulmonary thromboembolism
    Carlos A C Dias-Junior
    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av Bandeirantes, 3900, 14049 900, Ribeirao Preto, SP, Brazil
    Free Radic Biol Med 41:1764-70. 2006
    ..These findings suggest a new therapeutic application for nitrite and support emerging evidence for a surprisingly potent and potentially physiological vasoactivity of nitrite...
  3. pmc Hemodynamic and functional assessment of patients with sickle cell disease and pulmonary hypertension
    Anastasia Anthi
    Vascular Medicine Branch, Clinical Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 2089, USA
    Am J Respir Crit Care Med 175:1272-9. 2007
    ..Although pulmonary hypertension (PH) is a common complication of sickle cell disease (SCD) associated with high mortality, there exist few data characterizing hemodynamics and cardiopulmonary function in this population...
  4. pmc Inhaled nitric oxide augments nitric oxide transport on sickle cell hemoglobin without affecting oxygen affinity
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 104:937-45. 1999
    ..The levels of nitrosylated hemoglobin are too low to affect overall hemoglobin oxygen affinity, but augmented NO transport to the microvasculature seems a promising strategy for improving microvascular perfusion...
  5. ncbi request reprint Divergent nitric oxide bioavailability in men and women with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circulation 107:271-8. 2003
    ..Although reduced endothelial nitric oxide (NO) bioavailability has been demonstrated in arteriosclerotic vascular disease, the integrity of this system in sickle cell disease remains uncertain...
  6. ncbi request reprint Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:557-61. 2006
  7. ncbi request reprint Nitric oxide's reactions with hemoglobin: a view through the SNO-storm
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 9:496-500. 2003
  8. ncbi request reprint The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, Laboratory of Chemical Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Free Radic Biol Med 36:707-17. 2004
    ..This newly described role of hemoglobin as a nitrite reductase is discussed in the context of blood flow regulation, oxygen sensing, and nitrite-based therapeutics...
  9. pmc Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndrome
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, and Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 116:2330-2. 2006
    ..Diseases associated with NO resistance would appear to be ideally suited for therapies directed at restoring redox homeostasis, sGC activity, and NO sensitivity...
  10. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...
  11. pmc Haldane, hot dogs, halitosis, and hypoxic vasodilation: the emerging biology of the nitrite anion
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 113:19-21. 2004
    ..Nitrite levels in blood reflect NO production from endothelial NO synthase enzymes, and recent data suggest that nitrite contributes to blood flow regulation by reaction with deoxygenated hemoglobin and tissue heme proteins to form NO...
  12. ncbi request reprint Pulmonary hypertension as a risk factor for death in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    N Engl J Med 350:886-95. 2004
    ..The prevalence of pulmonary hypertension in adults with sickle cell disease, the mechanism of its development, and its prospective prognostic significance are unknown...
  13. ncbi request reprint Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation
    Mark T Gladwin
    Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    Am J Physiol Heart Circ Physiol 291:H2026-35. 2006
    ..An expanded consideration of nitrite as a hypoxia-dependent intrinsic signaling molecule has opened up a new field of research and therapeutic opportunities for diseases associated with regional hypoxia and vasoconstriction...
  14. pmc The functional nitrite reductase activity of the heme-globins
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
    Blood 112:2636-47. 2008
    ..We will review these reactions in the biologic framework of hypoxic signaling in blood and the heart...
  15. ncbi request reprint S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric function
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    J Biol Chem 277:27818-28. 2002
    ....
  16. pmc Relative role of heme nitrosylation and beta-cysteine 93 nitrosation in the transport and metabolism of nitric oxide by hemoglobin in the human circulation
    M T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:9943-8. 2000
    ....
  17. ncbi request reprint Nitric oxide transport on sickle cell hemoglobin: where does it bind?
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Res 35:175-80. 2001
    ....
  18. ncbi request reprint Retinoic acid reduces p11 protein levels in bronchial epithelial cells by a posttranslational mechanism
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Am J Physiol Lung Cell Mol Physiol 279:L1103-9. 2000
    ..006). Therefore, RA reduces p11 protein expression and increases PLA(2) activity and AA release...
  19. ncbi request reprint Nitric oxide donor properties of hydroxyurea in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, Bethesda, MD 20892 1662, USA
    Br J Haematol 116:436-44. 2002
    ..The role of NO in the induction of fetal haemoglobin and possible synergy between NO donor therapy and classic cytostatic and differentiating medications should be explored...
  20. pmc Effects of inhaled nitric oxide on regional blood flow are consistent with intravascular nitric oxide delivery
    R O Cannon
    Cardiology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892 1650, USA
    J Clin Invest 108:279-87. 2001
    ..This effect may have application for the treatment of diseases characterized by endothelial dysfunction...
  21. pmc Role of circulating nitrite and S-nitrosohemoglobin in the regulation of regional blood flow in humans
    M T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:11482-7. 2000
    ....
  22. ncbi request reprint Noninvasive determination of spatially resolved and time-resolved tissue perfusion in humans during nitric oxide inhibition and inhalation by use of a visible-reflectance hyperspectral imaging technique
    K J Zuzak
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda MD 20892-0510, USA
    Circulation 104:2905-10. 2001
    ..This imaging method may provide an effective approach for time-resolved noninvasive monitoring of skin hemoglobin oxygen saturation and assessment of responses to therapeutic interventions in patients with vascular disease...
  23. ncbi request reprint Nitric oxide therapy in sickle cell disease
    M T Gladwin
    Critical Care Medicine Department, Warren G. Magnuson Clinical Center, NIH, Bethesda, MD 20892-1662, USA
    Semin Hematol 38:333-42. 2001
    ..While direct evidence for a clinical benefit of NO therapy in sickle cell disease has not been reported, studies are underway to determine if inhaled NO will reduce the substantial morbidity and mortality suffered by these patients...
  24. ncbi request reprint Regional cerebral hyperperfusion and nitric oxide pathway dysregulation in Fabry disease: reversal by enzyme replacement therapy
    D F Moore
    Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1260, USA
    Circulation 104:1506-12. 2001
    ..CONCLUSIONS: These findings suggest a chronic alteration of the nitric oxide pathway in Fabry disease, with critical protein nitration that is reversible with enzyme replacement therapy...
  25. pmc Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure
    M K Holly
    Renal Diagnostics and Therapeutics Unit, NIDDK, Bethesda, Maryland, USA
    Kidney Int 70:496-506. 2006
    ..This model allowed us to use DIGE to find changes in urinary proteins and this approach identified a potential biomarker and drug target - meprin-1-alpha...
  26. ncbi request reprint Disorders of ciliary motility
    M J Cowan
    Department of Critical Care Medicine, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    Am J Med Sci 321:3-10. 2001
    ..Late stages of the disease may require surgical intervention for bronchiectasis or lung transplant for end-stage lung disease...
  27. ncbi request reprint Endothelial progenitor cell mobilization and increased intravascular nitric oxide in patients undergoing cardiac rehabilitation
    Jonathan D Paul
    Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    J Cardiopulm Rehabil Prev 27:65-73. 2007
    ....
  28. pmc Severe pulmonary hypertension in an adolescent with sickle cell disease
    James G Taylor
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1476, USA
    Am J Hematol 83:71-2. 2008
  29. ncbi request reprint Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:12916-27. 2007
    ....
  30. pmc Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypes
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Drive, Building 10CRC 5 5140, Bethesda, MD 20892 1476, USA
    Blood Rev 21:37-47. 2007
    ..Some of these drugs are now being studied in clinical trials...
  31. pmc Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension, and nitric oxide scavenging by cell-free hemoglobin
    Jose Villagra
    Vascular Medicine Branch of National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1476, USA
    Blood 110:2166-72. 2007
    ..This supports a role for NO-based therapeutics for SCD vasculopathy. This trial was registered at www.clinicaltrials.gov as no. NCT00352430...
  32. ncbi request reprint Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasis
    Sruti Shiva
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 2:486-93. 2006
    ....
  33. ncbi request reprint Measurement of nitric oxide levels in the red cell: validation of tri-iodide-based chemiluminescence with acid-sulfanilamide pretreatment
    Xunde Wang
    Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 281:26994-7002. 2006
    ....
  34. ncbi request reprint Hemoglobin oxygen fractional saturation regulates nitrite-dependent vasodilation of aortic ring bioassays
    T Scott Isbell
    Department of Pathology, and Center for Free Radical Biology, University of Alabama at Birmingham, Alabama 35296, USA
    Am J Physiol Heart Circ Physiol 293:H2565-72. 2007
    ....
  35. ncbi request reprint Nitrite infusion in humans and nonhuman primates: endocrine effects, pharmacokinetics, and tolerance formation
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Circulation 116:1821-31. 2007
    ..However, important questions of potency, kinetics, mechanism of action, and possible induction of tolerance remain unanswered...
  36. pmc Nitrite reductase activity of hemoglobin as a systemic nitric oxide generator mechanism to detoxify plasma hemoglobin produced during hemolysis
    Peter C Minneci
    Department of Surgery, The Children s Institute for Surgical Science, The Children s Hospital of Philadelphia, PA, USA
    Am J Physiol Heart Circ Physiol 295:H743-54. 2008
    ..Nitrite infusions may promote NO generation from Hb while maintaining oxygen delivery; this effect could be harnessed to treat hemolytic conditions and to detoxify Hb-based blood substitutes...
  37. pmc Nitrite anion provides potent cytoprotective and antiapoptotic effects as adjunctive therapy to reperfusion for acute myocardial infarction
    Felix M Gonzalez
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1061, USA
    Circulation 117:2986-94. 2008
    ....
  38. pmc The proverbial chicken or the egg? Dissection of the role of cell-free hemoglobin versus reactive oxygen species in sickle cell pathophysiology
    Megan L Krajewski
    Am J Physiol Heart Circ Physiol 295:H4-7. 2008
  39. pmc Prevalence and risk factors for pulmonary artery systolic hypertension among sickle cell disease patients in Nigeria
    Zakari Y Aliyu
    Department of Medicine and Center for Sickle Cell Disease, Howard University, Washington, District of Columbia 20060, USA
    Am J Hematol 83:485-90. 2008
    ..The inclusion of African sites in sickle cell pulmonary hypertension clinical trials should be encouraged...
  40. doi request reprint Sickle cell disease vasculopathy: a state of nitric oxide resistance
    Katherine C Wood
    Vascular Medicine Branch, National Heart Lung Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 44:1506-28. 2008
    ..New therapeutic strategies to limit intravascular hemolysis and ROS generation and increase NO bioavailability are discussed...
  41. pmc Chronic hyper-hemolysis in sickle cell anemia: association of vascular complications and mortality with less frequent vasoocclusive pain
    James G Taylor
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 3:e2095. 2008
    ..We have previously reported that intense hemolysis is associated with increased risk of vascular complications in a small cohort of adults with sickle cell disease. These observations have not been validated in other populations...
  42. pmc The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin
    Xiaojun He
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 44:1420-32. 2008
    ..These results support the notion that Angeli's salt or a similar compound could be used to effectively treat conditions associated with intravascular hemolysis...
  43. pmc Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins
    Xunde Wang
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 44:1362-72. 2008
    ..The addition of copper to ascorbate allows for a simple assay modification that dramatically increases sensitivity while maintaining specificity...
  44. doi request reprint The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics
    Jon O Lundberg
    Department of Physiology and Pharmacology, Karolinska Institute, SE 171 77 Stockholm, Sweden
    Nat Rev Drug Discov 7:156-67. 2008
    ....
  45. ncbi request reprint Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:3628-38. 2008
    ..From a pathophysiological standpoint, under conditions where HbS polymers form, the rate of nitrite reduction is reduced compared with HbA and solution-phase HbS, indicating that HbS polymers reduce nitrite more slowly...
  46. ncbi request reprint Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin
    Swati Basu
    Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
    Nat Chem Biol 3:785-94. 2007
    ....
  47. ncbi request reprint Sickle cell disease and pulmonary hypertension in Africa: a global perspective and review of epidemiology, pathophysiology, and management
    Zakari Y Aliyu
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    Am J Hematol 83:63-70. 2008
    ..There is clearly a need to include Africa and other parts of the world with high SCD prevalence in future comprehensive studies on the epidemiology and treatment of end organ complications of an aging SCD population world-wide...
  48. ncbi request reprint Pulmonary vascular effects of red blood cells containing S-nitrosated hemoglobin
    Steven Deem
    Dept of Anesthesiology, PO Box 359724, Harborview Medical Center, Seattle, WA 98104 2499, USA
    Am J Physiol Heart Circ Physiol 287:H2561-8. 2004
    ....
  49. ncbi request reprint Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator
    Christian J Hunter
    Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892 1662, USA
    Nat Med 10:1122-7. 2004
    ....
  50. ncbi request reprint How do red blood cells dilate blood vessels?--Reply
    Daniel B Kim-Shapiro
    Circ Res 95:e10. 2004
  51. ncbi request reprint The perfusion paradox and vascular instability in sickle cell disease
    Karl A Nath
    Division of Nephrology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Microcirculation 11:179-93. 2004
    ..Finally, the possibility that the induction of heme oxygenase-1, recently described in SCD, may confer a protective response in the vasculature and other tissues is discussed...
  52. pmc Biological activity of nitric oxide in the plasmatic compartment
    Xunde Wang
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Proc Natl Acad Sci U S A 101:11477-82. 2004
    ..The studies further suggest that alternative NO reaction end products in plasma, such as nitrite, N-nitrosamines, iron-nitrosyls, and nitrated lipids, should be evaluated in blood NO transport along the vasculature...
  53. ncbi request reprint NO contest: nitrite versus S-nitroso-hemoglobin
    Mark T Gladwin
    Circ Res 94:851-5. 2004
  54. ncbi request reprint Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
    Kenyatta Cosby
    Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
    Nat Med 9:1498-505. 2003
    ..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation...
  55. ncbi request reprint Hemolytic anemia-associated pulmonary hypertension of sickle cell disease and the nitric oxide/arginine pathway
    Maria L Jison
    Am J Respir Crit Care Med 168:3-4. 2003
  56. ncbi request reprint Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide
    Karel J Zuzak
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases NIH, 9000 Rockville Pike, Bethesda, MD 20892 0510, USA
    Am J Physiol Heart Circ Physiol 285:H1183-9. 2003
    ....
  57. ncbi request reprint Methodologies for the sensitive and specific measurement of S-nitrosothiols, iron-nitrosyls, and nitrite in biological samples
    Benjamin K Yang
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Free Radic Res 37:1-10. 2003
  58. ncbi request reprint An emerging role for nitric oxide in sickle cell disease vascular homeostasis and therapy
    Christopher D Reiter
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    Curr Opin Hematol 10:99-107. 2003
    ..Possible effects of chronic nitric oxide-based therapies on erythrocyte density, pulmonary artery pressures, and fetal hemoglobin induction deserve study...
  59. ncbi request reprint Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
    Christopher D Reiter
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 8:1383-9. 2002
    ..Decompartmentalization of hemoglobin and subsequent dioxygenation of nitric oxide may explain the vascular complications shared by acute and chronic hemolytic disorders...
  60. ncbi request reprint Nitric oxide (NO) in normal and hypoxic vascular regulation of the spiny dogfish, Squalus acanthias
    Kai E Swenson
    Mt Desert Island Biological Laboratory, Salsbury Cove, Maine 04672, USA
    J Exp Zool A Comp Exp Biol 303:154-60. 2005
    ..In acute hypoxia, there is a rapid up-regulation of vascular NO production that appears to be responsible for hypoxic vasodilation...
  61. ncbi request reprint Hemolytic anemia-associated pulmonary hypertension in sickle cell disease
    Elaina E Lin
    National Institutes of Health, 31 Center Drive, Bethesda, MD 20892, USA
    Curr Hematol Rep 4:117-25. 2005
    ..Finally, we review suggested therapies including the established treatments and new pulmonary vasodilator and remodeling agents in the management of pulmonary hypertension in hemolytic anemias...
  62. pmc Cerebrovascular disease associated with sickle cell pulmonary hypertension
    Gregory J Kato
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1476, USA
    Am J Hematol 81:503-10. 2006
    ..Clinicians should suspect occult cerebrovascular disease in sickle cell patients with pulmonary hypertension...
  63. ncbi request reprint Lopinavir-ritonavir: effects on endothelial cell function in healthy subjects
    Jessica R Grubb
    Critical Care Medical Department, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 193:1516-9. 2006
    ..Thus, in this small cohort of subjects, short-term treatment with lopinavir-ritonavir does not appear to directly promote endothelial cell dysfunction...
  64. pmc Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobin
    Peter C Minneci
    Critical Care Medicine Department, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 115:3409-17. 2005
    ....
  65. pmc Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation
    Jack H Crawford
    Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, 35294, USA
    Blood 107:566-74. 2006
    ..These data support a function for RBC hemoglobin as an allosterically and redox-regulated nitrite reductase whose "enzyme activity" couples hypoxia to increased NO-dependent blood flow...
  66. ncbi request reprint Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenation
    Ivan Azarov
    Department of Physics, School of Medicine, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 280:39024-32. 2005
    ..These observations may have important physiological implications within the microvasculature and for pathophysiological disruption of nitric oxide homeostasis in diseases...
  67. pmc Sildenafil therapy in patients with sickle cell disease and pulmonary hypertension
    Roberto F Machado
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart Lung and Blood Institute, Bethesda, MD, USA
    Br J Haematol 130:445-53. 2005
    ....
  68. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  69. pmc Dysregulated arginine metabolism, hemolysis-associated pulmonary hypertension, and mortality in sickle cell disease
    Claudia R Morris
    Department of Emergency Medicine, Children s Hospital and Research Center at Oakland, CA 94609, USA
    JAMA 294:81-90. 2005
    ..We hypothesized that increased arginase activity and dysregulated arginine metabolism contribute to endothelial dysfunction, pulmonary hypertension, and patient outcomes...
  70. ncbi request reprint Hemoglobin as a nitrite reductase regulating red cell-dependent hypoxic vasodilation
    Mark T Gladwin
    Am J Respir Cell Mol Biol 32:363-6. 2005
  71. pmc Erythrocytes are the major intravascular storage sites of nitrite in human blood
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 106:734-9. 2005
    ..These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit...
  72. ncbi request reprint Effects of S-nitrosation and cross-linking of hemoglobin on hypoxic pulmonary vasoconstriction in isolated rat lungs
    Steven Deem
    Department of Anesthesiology, University of Washington and the Veterans Affairs Puget Sound Health Care Center, Seattle, Wash, USA
    Circ Res 91:626-32. 2002
    ..However, release of NO from SNO-Hb was not influenced by deoxygenation-mediated allosteric changes in Hb across a broad range of oxyHb saturation. Cross-linking of Hb does not limit its pulmonary vasoconstrictor effects...