Maria Lucia Ghirardi

Summary

Affiliation: National Renewable Energy Laboratory
Country: USA

Publications

  1. ncbi request reprint A comparison of hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under different growth conditions
    Sergey Kosourov
    Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142290, Russia
    J Biotechnol 128:776-87. 2007
  2. doi request reprint Photobiological hydrogen-producing systems
    Maria Lucia Ghirardi
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Chem Soc Rev 38:52-61. 2009
  3. ncbi request reprint Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Annu Rev Plant Biol 58:71-91. 2007
  4. ncbi request reprint Approaches to developing biological H(2)-photoproducing organisms and processes
    M L Ghirardi
    National Renewable Energy Laboratory, Golden, CO, USA
    Biochem Soc Trans 33:70-2. 2005
  5. ncbi request reprint Hydrogen production by photosynthetic green algae
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Indian J Biochem Biophys 43:201-10. 2006
  6. ncbi request reprint Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii
    M C Posewitz
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Biochem Soc Trans 33:102-4. 2005
  7. ncbi request reprint Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity
    Valeria V Makarova
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Photosynth Res 94:79-89. 2007
  8. pmc Atomic resolution modeling of the ferredoxin:[FeFe] hydrogenase complex from Chlamydomonas reinhardtii
    Christopher H Chang
    National Renewable Energy Laboratory, Golden, Colorado, USA
    Biophys J 93:3034-45. 2007
  9. ncbi request reprint Hydrogen fuel production by transgenic microalgae
    Anastasios Melis
    University of California, Department of Plant and Microbial Biology, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Adv Exp Med Biol 616:110-21. 2007
  10. doi request reprint Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures
    Tatyana V Laurinavichene
    Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142290, Russia
    J Biotechnol 134:275-7. 2008

Collaborators

  • Paul W King
  • KLAUS J SCHULTEN
  • Thomas Happe
  • Anastasios Melis
  • Christopher H Chang
  • Michael Seibert
  • Sergey Kosourov
  • Matthew C Posewitz
  • Alexander S Fedorov
  • Anatoly Tsygankov
  • Boris K Semin
  • Hai Long
  • Tatyana V Laurinavichene
  • Lauren E Nagy
  • Valeria V Makarova
  • M C Posewitz
  • Sharon L Smolinski
  • John J Hahn
  • Liping Zhang
  • Marc Forestier
  • Anatoly A Tsygankov
  • Sergey N Kosourov
  • Kwiseon Kim
  • Scott Plummer
  • Tatiana E Krendeleva
  • Dianne Ahmann
  • Elena Patrusheva
  • Galina P Kukarskikh
  • Richard T Sayre
  • Andrei B Rubin
  • Jonathan E Meuser
  • A R Ginley
  • Valeriya Makarova
  • S L Smolinski
  • R Davis Smith
  • M Seibert
  • Saradadevi Kanakagiri
  • William A Jacoby
  • Matthew Posewitz
  • Sarah Schwarzer

Detail Information

Publications22

  1. ncbi request reprint A comparison of hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under different growth conditions
    Sergey Kosourov
    Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142290, Russia
    J Biotechnol 128:776-87. 2007
    ..These results suggest ways of engineering algae to improve H(2) production, which in turn may have a positive impact on the economics of applied systems for H(2) production...
  2. doi request reprint Photobiological hydrogen-producing systems
    Maria Lucia Ghirardi
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Chem Soc Rev 38:52-61. 2009
    ....
  3. ncbi request reprint Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Annu Rev Plant Biol 58:71-91. 2007
    ..Some of this effort is clearly driven by the potential for using these enzymes in future biological or biohybrid systems to produce renewable fuel or in fuel cell applications...
  4. ncbi request reprint Approaches to developing biological H(2)-photoproducing organisms and processes
    M L Ghirardi
    National Renewable Energy Laboratory, Golden, CO, USA
    Biochem Soc Trans 33:70-2. 2005
    ....
  5. ncbi request reprint Hydrogen production by photosynthetic green algae
    Maria L Ghirardi
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Indian J Biochem Biophys 43:201-10. 2006
    ..In this review, current biochemical understanding of this reaction in green algae, and some of the major challenges facing the development of future commercial algal photobiological systems for H2 production have been discussed...
  6. ncbi request reprint Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii
    M C Posewitz
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Biochem Soc Trans 33:102-4. 2005
    ..reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated...
  7. ncbi request reprint Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity
    Valeria V Makarova
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Photosynth Res 94:79-89. 2007
    ..The more compromised the PSII photochemical activity, the more dramatic was the effect of sulfur deprivation on the H2-production process, which depends both on the presence of residual PSII activity and the amount of stored starch...
  8. pmc Atomic resolution modeling of the ferredoxin:[FeFe] hydrogenase complex from Chlamydomonas reinhardtii
    Christopher H Chang
    National Renewable Energy Laboratory, Golden, Colorado, USA
    Biophys J 93:3034-45. 2007
    ....
  9. ncbi request reprint Hydrogen fuel production by transgenic microalgae
    Anastasios Melis
    University of California, Department of Plant and Microbial Biology, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Adv Exp Med Biol 616:110-21. 2007
    ....
  10. doi request reprint Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures
    Tatyana V Laurinavichene
    Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142290, Russia
    J Biotechnol 134:275-7. 2008
    ..Furthermore, it is also possible to prolong the duration of H2 production by cycling immobilized cells between minus and plus sulfate conditions...
  11. ncbi request reprint Application of gene-shuffling for the rapid generation of novel [FeFe]-hydrogenase libraries
    Lauren E Nagy
    Environmental Science and Engineering Division, Colorado School of Mines, Golden, CO 80401, USA
    Biotechnol Lett 29:421-30. 2007
    ..Moreover, we demonstrate that a single set of [FeFe]-hydrogenase maturation proteins is sufficient for the heterologous assembly of the bioinorganic active site of several native and shuffled [FeFe]-hydrogenases...
  12. pmc Brownian dynamics and molecular dynamics study of the association between hydrogenase and ferredoxin from Chlamydomonas reinhardtii
    Hai Long
    National Renewable Energy Laboratory, Golden, Colorado, USA
    Biophys J 95:3753-66. 2008
    ..The ferredoxin appeared to form reasonable complexes with the hydrogenase in multiple orientations, some of which were good candidates for inclusion in a transition state ensemble of configurations for electron transfer...
  13. pmc Functional studies of [FeFe] hydrogenase maturation in an Escherichia coli biosynthetic system
    Paul W King
    Basic Sciences Center, NREL, Golden, CO 80401, USA
    J Bacteriol 188:2163-72. 2006
    ..Together, these results demonstrate that the catalytic domain and a functionally complete set of Hyd maturation proteins are fundamental to achieving biosynthesis of catalytic [FeFe] hydrogenases...
  14. ncbi request reprint Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: Effects of culture parameters
    Sergey Kosourov
    Basic Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA
    Biotechnol Bioeng 78:731-40. 2002
    ....
  15. ncbi request reprint Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures
    Sergey Kosourov
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U S A
    Plant Cell Physiol 44:146-55. 2003
    ..Carbon balances demonstrate that alternative metabolic pathways predominate at different pHs, and these depend on whether residual photosynthetic activity is present or not...
  16. ncbi request reprint Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions
    Marc Forestier
    National Renewable Energy Laboratory, Golden, CO 80401, USA
    Eur J Biochem 270:2750-8. 2003
    ..To our knowledge, this work represents the first systematic study of expression of two algal [Fe]-hydrogenases in the same organism...
  17. ncbi request reprint Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase
    Matthew C Posewitz
    National Renewable Energy Laboratory, Colorado School of Mines, Golden, CO 80401, USA
    J Biol Chem 279:25711-20. 2004
    ..This represents the first report on the nature of the accessory genes required for the maturation of an active [Fe] hydrogenase...
  18. ncbi request reprint Effect of process variables on photosynthetic algal hydrogen production
    John J Hahn
    Department of Chemical Engineering, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biotechnol Prog 20:989-91. 2004
    ..29 mL of hydrogen per milliliter of suspension. This was measured at atmospheric pressure during a 96 h production cycle. This corresponds to an average hydrogen production rate of 0.12 mmol/mL.h...
  19. pmc Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii
    Matthew C Posewitz
    National Renewable Energy Laboratory, Golden, Colorado 80401, USA
    Plant Cell 16:2151-63. 2004
    ..reinhardtii H(2) photoproduction. Moreover, the results indicate that mere anaerobiosis is not sufficient to maintain hydrogenase gene expression without the underlying physiology, an important aspect of which is starch metabolism...
  20. ncbi request reprint Continuous hydrogen photoproduction by Chlamydomonas reinhardtii: using a novel two-stage, sulfate-limited chemostat system
    Alexander S Fedorov
    National Renewable Energy Laboratory, Basic Science Center, 1617 Cole Boulevard, Golden, CO 80401, USA
    Appl Biochem Biotechnol 121:403-12. 2005
    ..The dependence of the H2 production rate on sulfate concentration in the medium, dilution rates in the PhBRs, and incident light intensity is reported...
  21. ncbi request reprint The effect of sulfur re-addition on H(2) photoproduction by sulfur-deprived green algae
    Sergey Kosourov
    National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, USA
    Photosynth Res 85:295-305. 2005
    ..These results show that the presence of a more oxidized PQ pool releases some of the down-regulation of electron transport caused by the anaerobic conditions...
  22. ncbi request reprint Blocking of electron donation by Mn(II) to Y(Z*) following incubation of Mn-depleted photosystem II membranes with Fe(II) in the light
    Boris K Semin
    Basic Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401, USA
    Biochemistry 41:5854-64. 2002
    ..Since hydroxylamine does not reduce the Fe [> or =Fe(III)] specifically bound to the HA(Z) site, NH2OH must donate to Y(Z*) through its own site or directly to P680+...