Burhan I Ghanayem

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Diet-induced obesity in male mice is associated with reduced fertility and potentiation of acrylamide-induced reproductive toxicity
    Burhan I Ghanayem
    Laboratory of Pharmacology, Biostatistics Branch, and Cellular and Molecular Pathology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 82:96-104. 2010
  2. ncbi request reprint Comparison of germ cell mutagenicity in male CYP2E1-null and wild-type mice treated with acrylamide: evidence supporting a glycidamide-mediated effect
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 72:157-63. 2005
  3. ncbi request reprint Absence of acrylamide-induced genotoxicity in CYP2E1-null mice: evidence consistent with a glycidamide-mediated effect
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mutat Res 578:284-97. 2005
  4. ncbi request reprint Acrylonitrile is a multisite carcinogen in male and female B6C3F1 mice
    Burhan I Ghanayem
    Environmental Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 68:59-68. 2002
  5. ncbi request reprint Hemolytic anemia, thrombosis, and infarction in male and female F344 rats following gavage exposure to 2-butoxyethanol
    B I Ghanayem
    National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Exp Toxicol Pathol 53:97-105. 2001
  6. ncbi request reprint Using cytochrome P-450 gene knock-out mice to study chemical metabolism, toxicity, and carcinogenicity
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 28:839-50. 2000
  7. ncbi request reprint Role of cytochrome P-450 2E1 in methacrylonitrile metabolism and disposition
    B I Ghanayem
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 289:1054-9. 1999
  8. ncbi request reprint Inhibition of urethane-induced carcinogenicity in cyp2e1-/- in comparison to cyp2e1+/+ mice
    Burhan I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 95:331-9. 2007
  9. pmc Effect of dose volume on the toxicokinetics of acrylamide and its metabolites and 2-deoxy-D-glucose
    Burhan I Ghanayem
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 37:259-63. 2009
  10. ncbi request reprint Investigation of xenobiotics metabolism, genotoxicity, and carcinogenicity using Cyp2e1(-/-) mice
    Burhan I Ghanayem
    Metabolism and Exposure Markers Section, Laboratory of Pharmacology and Chemistry, National Toxicology Program, NIEHS, NIH, RTP, NC 27709, USA
    Curr Drug Metab 8:728-49. 2007

Collaborators

Detail Information

Publications36

  1. pmc Diet-induced obesity in male mice is associated with reduced fertility and potentiation of acrylamide-induced reproductive toxicity
    Burhan I Ghanayem
    Laboratory of Pharmacology, Biostatistics Branch, and Cellular and Molecular Pathology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 82:96-104. 2010
    ..This work demonstrated that diet-induced obesity in mice caused a significant reduction in male fertility and exacerbated AA-induced reproductive toxicity and germ cell mutagenicity...
  2. ncbi request reprint Comparison of germ cell mutagenicity in male CYP2E1-null and wild-type mice treated with acrylamide: evidence supporting a glycidamide-mediated effect
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 72:157-63. 2005
    ..Thus, CYP2E1 polymorphisms in human populations, resulting in variable enzyme metabolic activities, may produce differential susceptibilities to acrylamide toxicities...
  3. ncbi request reprint Absence of acrylamide-induced genotoxicity in CYP2E1-null mice: evidence consistent with a glycidamide-mediated effect
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mutat Res 578:284-97. 2005
    ....
  4. ncbi request reprint Acrylonitrile is a multisite carcinogen in male and female B6C3F1 mice
    Burhan I Ghanayem
    Environmental Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 68:59-68. 2002
    ..This was also considered an equivocal result. In conclusion, these studies demonstrated that acrylonitrile causes multiple carcinogenic effects after gavage administration to male and female B6C3F1 mice for 2 years...
  5. ncbi request reprint Hemolytic anemia, thrombosis, and infarction in male and female F344 rats following gavage exposure to 2-butoxyethanol
    B I Ghanayem
    National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Exp Toxicol Pathol 53:97-105. 2001
    ..Thrombosis may be related to intravascular hemolysis, which could be triggered by procoagulant release and/or alterations in erythrocyte morphology, as well as increased rigidity...
  6. ncbi request reprint Using cytochrome P-450 gene knock-out mice to study chemical metabolism, toxicity, and carcinogenicity
    B I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 28:839-50. 2000
    ....
  7. ncbi request reprint Role of cytochrome P-450 2E1 in methacrylonitrile metabolism and disposition
    B I Ghanayem
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 289:1054-9. 1999
    ..It is therefore concluded that MAN oxidative metabolites such as the epoxide intermediate have greater reactivity than parent MAN...
  8. ncbi request reprint Inhibition of urethane-induced carcinogenicity in cyp2e1-/- in comparison to cyp2e1+/+ mice
    Burhan I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 95:331-9. 2007
    ....
  9. pmc Effect of dose volume on the toxicokinetics of acrylamide and its metabolites and 2-deoxy-D-glucose
    Burhan I Ghanayem
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 37:259-63. 2009
    ....
  10. ncbi request reprint Investigation of xenobiotics metabolism, genotoxicity, and carcinogenicity using Cyp2e1(-/-) mice
    Burhan I Ghanayem
    Metabolism and Exposure Markers Section, Laboratory of Pharmacology and Chemistry, National Toxicology Program, NIEHS, NIH, RTP, NC 27709, USA
    Curr Drug Metab 8:728-49. 2007
    ..In conclusion, the current review showed that Cyp2e1-/- mice are a valuable animal model for the investigation of the metabolic and molecular basis of toxicity, genotoxicity, and carcinogenicity of xenobiotics...
  11. ncbi request reprint Differential metabolism of acrylonitrile to cyanide is responsible for the greater sensitivity of male vs female mice: role of CYP2E1 and epoxide hydrolases
    Brian Chanas
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Toxicol Appl Pharmacol 193:293-302. 2003
    ..Further, higher expression of CYP2E1 and EH in male mice may contribute to greater formation of CEO and its subsequent metabolism to yield cyanide, respectively...
  12. ncbi request reprint Characterization of the toxicity, mutagenicity, and carcinogenicity of methacrylonitrile in F344 Rats and B6C3F1 mice
    Abraham Nyska
    Laboratory of Experimental Pathology, MD B3 06, Environmental Toxicology Program, National Institute of Environmental Health Sciences NIEHS, National Institutes of Health, PO Box 12233, Research Triangle Park, NC 27709, USA
    Arch Toxicol 77:233-42. 2003
    ..Methacrylonitrile-related non-neoplastic lesions were seen in the nose and liver of rats...
  13. doi request reprint Diet-induced obesity is associated with hyperleptinemia, hyperinsulinemia, hepatic steatosis, and glomerulopathy in C57Bl/6J mice
    Undi Hoffler
    Laboratory of Pharmacology, National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709, USA
    Endocrine 36:311-25. 2009
    ....
  14. ncbi request reprint Role of CYP2E1 in the epoxidation of acrylamide to glycidamide and formation of DNA and hemoglobin adducts
    Burhan I Ghanayem
    Laboratory of Pharmacology and Chemistry, National Toxicology Program, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Toxicol Sci 88:311-8. 2005
    ..In conclusion, current work demonstrated that CYP2E1 is the primary enzyme responsible for the epoxidation of AA to GA, which leads to the formation of GA-DNA and HGB adducts...
  15. ncbi request reprint Increased bioaccumulation of urethane in CYP2E1-/- versus CYP2E1+/+ mice
    Undi Hoffler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 33:1144-50. 2005
    ..Comparison of the metabolism of ethyl-versus carbonyl-labeled urethane was necessary for tracing the source of CO(2) and led us to propose for the first time that C-hydroxylation is a likely pathway of urethane metabolism...
  16. ncbi request reprint Cytochrome P450 2E1 (CYP2E1) is essential for acrylonitrile metabolism to cyanide: comparative studies using CYP2E1-null and wild-type mice
    Hongbing Wang
    Laboratory of Pharmacology and Chemistry, Environmental Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Drug Metab Dispos 30:911-7. 2002
    ..Finally, this work confirmed that cyanide plays an essential role in the causation of the acute toxicity/mortality of AN...
  17. ncbi request reprint Comparative metabolism and disposition of trichloroethylene in Cyp2e1-/-and wild-type mice
    Dojung Kim
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 34:2020-7. 2006
    ....
  18. ncbi request reprint CYP2E1-catalyzed oxidation contributes to the sperm toxicity of 1-bromopropane in mice
    C Edwin Garner
    RTI International, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 76:496-505. 2007
    ..Overall, these data suggest that metabolism of 1-BrP is mediated in part by CYP2E1, and activation of 1BrP via this enzyme may contribute to the male reproductive toxicity of this chemical...
  19. ncbi request reprint Comparative metabolism of methacrylonitrile and acrylonitrile to cyanide using cytochrome P4502E1 and microsomal epoxide hydrolase-null mice
    L El Hadri
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, MD B3 10, Research Triangle Park, NC 27709, USA
    Toxicol Appl Pharmacol 205:116-25. 2005
    ..female treated with either nitrile. Higher blood cyanide levels in male vs. female mice and in MAN- vs. AN-treated mice may explain the gender-related differences in the toxicity of these chemicals and the greater potency of MAN vs. AN...
  20. ncbi request reprint Effect of methacrylonitrile on cytochrome P-450 2E1 (CYP2E1) expression in male F344 rats
    Hongbing Wang
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Toxicol Environ Health A 65:523-37. 2002
    ..While the damage in the olfactory mucosa due to MAN treatment may not be explained by the observed induction of CYP2E1, it is possible that other CYPs may play a role in the in situ bioactivation of MAN...
  21. ncbi request reprint Disseminated thrombosis and bone infarction in female rats following inhalation exposure to 2-butoxyethanol
    A Nyska
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 27:287-94. 1999
    ..In conclusion, exposure to 500 ppm BE vapors caused acute disseminated thrombosis and bone infarction in female rats. Possible pathogenic mechanisms are discussed...
  22. ncbi request reprint Role of cytochrome P450 2E1 in the metabolism of acrylamide and acrylonitrile in mice
    S C Sumner
    Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709 2137, USA
    Chem Res Toxicol 12:1110-6. 1999
    ..P450 2E1-null mice may be an appropriate model for the investigation of the role of oxidative metabolism in the toxicity or carcinogenicity of these compounds...
  23. ncbi request reprint Human CYP2C19 is a major omeprazole 5-hydroxylase, as demonstrated with recombinant cytochrome P450 enzymes
    W G Karam
    National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 24:1081-7. 1996
    ..The current work also shows, for the first time, that other CYP2C enzymes (CYP2C8, CYP2C9, and CYP2C18) may contribute to OP hydroxylation at high substrate concentrations. In contrast, OP-S was formed principally by CYP3A4...
  24. ncbi request reprint Demonstration of a temporal relationship between ethyl acrylate-induced forestomach cell proliferation and carcinogenicity
    B I Ghanayem
    National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Toxicol Pathol 22:497-509. 1994
    ..Furthermore, a temporal relationship exists between EA-induced epithelial cell proliferation and forestomach carcinogenicity...
  25. ncbi request reprint A novel transversion in the intron 5 donor splice junction of CYP2C19 and a sequence polymorphism in exon 3 contribute to the poor metabolizer phenotype for the anticonvulsant drug S-mephenytoin
    G C Ibeanu
    National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 290:635-40. 1999
    ..Restriction fragment length polymerase chain reaction tests were developed to identify the new allelic variants...
  26. ncbi request reprint Evidence that CYP2C19 is the major (S)-mephenytoin 4'-hydroxylase in humans
    J A Goldstein
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Biochemistry 33:1743-52. 1994
    ..These data indicate that 2C19 is the principal determinant of (S)-mephenytoin 4'-hydroxylase activity in human liver...
  27. ncbi request reprint Identification of residues 99, 220, and 221 of human cytochrome P450 2C19 as key determinants of omeprazole activity
    G C Ibeanu
    NIEHS, National Institute of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 271:12496-501. 1996
    ..Our results thus indicate that amino acids 99, 220, and 221 are key residues that determine the specificity of P450 2C19 for omeprazole...
  28. ncbi request reprint Sustainability of forestomach hyperplasia in rats treated with ethyl acrylate for 13 weeks and regression after cessation of dosing
    B I Ghanayem
    National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Toxicol Pathol 19:273-9. 1991
    ..It, therefore, remains to be determined what duration of exposure or other factors are critical for reversibility or progression of EA-induced forestomach mucosal hyperplasia to neoplasia...
  29. ncbi request reprint Identification of new human CYP2C19 alleles (CYP2C19*6 and CYP2C19*2B) in a Caucasian poor metabolizer of mephenytoin
    G C Ibeanu
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    J Pharmacol Exp Ther 286:1490-5. 1998
    ..Thus, the new CYP2C19*6 allele contributes to the PM phenotype in Caucasians...
  30. ncbi request reprint Polymorphisms in human CYP2C8 decrease metabolism of the anticancer drug paclitaxel and arachidonic acid
    D Dai
    Laboratories of Pharmacology and Chemistry and Pulmonary and Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
    Pharmacogenetics 11:597-607. 2001
    ..This polymorphism has important clinical and physiological implications in individuals homozygous for this allele...
  31. ncbi request reprint An additional defective allele, CYP2C19*5, contributes to the S-mephenytoin poor metabolizer phenotype in Caucasians
    G C Ibeanu
    NIEHS, Research Triangle Park, NC 27709, USA
    Pharmacogenetics 8:129-35. 1998
    ....
  32. ncbi request reprint Inhibition of urethane-induced genotoxicity and cell proliferation in CYP2E1-null mice
    Undi Hoffler
    Department of Pharmacology, Meharry Medical College, Nashville, TN, USA
    Mutat Res 572:58-72. 2005
    ..In conclusion, these data demonstrated that CYP2E1-mediated metabolism of urethane, presumably via epoxide formation, is necessary for the induction of genotoxicity, and cell proliferation in the liver and lung of wild-type mice...
  33. ncbi request reprint Pulmonary bronchiolar cytotoxicity and formation of dichloroacetyl lysine protein adducts in mice treated with trichloroethylene
    Poh Gek Forkert
    Department of Anatomy and Cell Biology, Queen s University, Kingston, ON, Canada
    J Pharmacol Exp Ther 316:520-9. 2006
    ..These findings supported the contention that TCE bioactivation within the Clara cells, predominantly involving CYP2F2, correlated with bronchiolar cytotoxicity in mice...
  34. ncbi request reprint Bioactivation of 1,1-dichloroethylene to its epoxide by CYP2E1 and CYP2F enzymes
    Andrea C Simmonds
    Department of Anatomy and Cell Biology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Drug Metab Dispos 32:1032-9. 2004
    ..The results also demonstrated that CYP2E1 is the high-affinity enzyme involved in DCE bioactivation...
  35. ncbi request reprint Cytochrome P450 2E1 (CYP2E1) is the principal enzyme responsible for urethane metabolism: comparative studies using CYP2E1-null and wild-type mice
    Undi Hoffler
    Department of Pharmacology, Meharry Medical College, Nashville, Tennessee, USA
    J Pharmacol Exp Ther 305:557-64. 2003
    ..In conclusion, this work demonstrated that CYP2E1, not esterase, is the principal enzyme responsible for urethane metabolism...
  36. ncbi request reprint Bioactivation of 1,1-dichloroethylene by CYP2E1 and CYP2F2 in murine lung
    Andrea C Simmonds
    Department of Anatomy and Cell Biology, Queen s University, Kingston, ON, Canada K7L 3N6
    J Pharmacol Exp Ther 310:855-64. 2004
    ..These results showed that bioactivation of DCE in murine lung occurred via the catalytic activities of both CYP2E1 and CYP2F2 and that bioactivation by these enzymes mediated the lung toxicity...