Martin Gellert

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint V(D)J recombination: RAG proteins, repair factors, and regulation
    Martin Gellert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892 0540, USA
    Annu Rev Biochem 71:101-32. 2002
  2. pmc Ordered assembly of the V(D)J synaptic complex ensures accurate recombination
    Jessica M Jones
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5 Room 241, Bethesda, MD 20892, USA
    EMBO J 21:4162-71. 2002
  3. pmc Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:22487-92. 2010
  4. pmc Initial stages of V(D)J recombination: the organization of RAG1/2 and RSS DNA in the postcleavage complex
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 35:217-27. 2009
  5. pmc Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops
    Shikha Gupta
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 19:72-8. 2012
  6. pmc Autoubiquitylation of the V(D)J recombinase protein RAG1
    Jessica M Jones
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5 Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:15446-51. 2003
  7. pmc Inverse transposition by the RAG1 and RAG2 proteins: role reversal of donor and target DNA
    I Hung Shih
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    EMBO J 21:6625-33. 2002
  8. pmc Requirements for DNA hairpin formation by RAG1/2
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:3078-83. 2007
  9. pmc An activation-induced cytidine deaminase-independent mechanism of secondary VH gene rearrangement in preimmune human B cells
    Nancy S Longo
    Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes andDigestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1560, USA
    J Immunol 181:7825-34. 2008
  10. pmc Effect of HIV integrase inhibitors on the RAG1/2 recombinase
    Meni Melek
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:134-7. 2002

Collaborators

Detail Information

Publications14

  1. ncbi request reprint V(D)J recombination: RAG proteins, repair factors, and regulation
    Martin Gellert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892 0540, USA
    Annu Rev Biochem 71:101-32. 2002
    ..This transposition helps to explain the mechanism of RAG action and supports earlier proposals that V(D)J recombination evolved from an ancient mobile DNA element...
  2. pmc Ordered assembly of the V(D)J synaptic complex ensures accurate recombination
    Jessica M Jones
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5 Room 241, Bethesda, MD 20892, USA
    EMBO J 21:4162-71. 2002
    ..Additional cellular factors such as chromatin may ensure that RAG1/2 first assembles on a 12 RSS, and then a free 23 RSS enters to activate cleavage...
  3. pmc Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:22487-92. 2010
    ..Thus a negative regulatory function of the noncore regions of RAG1/2 limits the RAG endonuclease activity in the absence of an activating methylated histone tail bound to the complex...
  4. pmc Initial stages of V(D)J recombination: the organization of RAG1/2 and RSS DNA in the postcleavage complex
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 35:217-27. 2009
    ..These first images of the V(D)J recombinase in its postcleavage state provide a framework for modeling RAG domains and their interactions with DNA...
  5. pmc Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops
    Shikha Gupta
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 19:72-8. 2012
    ..The C-terminal dimerization domains form an integral part of the MutS structure and coordinate asymmetrical ATP hydrolysis by Msh2 and Msh3 with mismatch binding to signal for repair...
  6. pmc Autoubiquitylation of the V(D)J recombinase protein RAG1
    Jessica M Jones
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5 Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:15446-51. 2003
    ..Disruption of the RING finger and certain RAG1 N-terminal truncations are associated with immunodeficiency in human patients, suggesting that RAG1's ubiquitin ligase is required for its biological role in lymphocyte development...
  7. pmc Inverse transposition by the RAG1 and RAG2 proteins: role reversal of donor and target DNA
    I Hung Shih
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    EMBO J 21:6625-33. 2002
    ..This aberrant activity provides another possible mechanism for some chromosomal translocations present in lymphoid tumors...
  8. pmc Requirements for DNA hairpin formation by RAG1/2
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:3078-83. 2007
    ..A W956A mutation in RAG1 had an inhibitory effect on both nicking and hairpin stages that could be rescued by abasic substrates. W956 is therefore a likely candidate for interacting with this base during hairpin formation...
  9. pmc An activation-induced cytidine deaminase-independent mechanism of secondary VH gene rearrangement in preimmune human B cells
    Nancy S Longo
    Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes andDigestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1560, USA
    J Immunol 181:7825-34. 2008
    ..The data suggest that V(H)4 replacement in preimmune human B cells is mediated by an AICDA-independent mechanism resulting from inefficient but selective RAG activity...
  10. pmc Effect of HIV integrase inhibitors on the RAG1/2 recombinase
    Meni Melek
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:134-7. 2002
    ..These results further underscore the similarities between RAG1/2 and integrase and suggest that certain integrase inhibitors may have the potential to interfere with aspects of B and T cell development...
  11. pmc Metabolic sensor AMPK directly phosphorylates RAG1 protein and regulates V(D)J recombination
    Jee Hyun Um
    Laboratory of Obesity and Aging Research, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 110:9873-8. 2013
    ..Our results suggest that V(D)J recombination can be regulated by AMPK activation, providing a potential new link between metabolic stress and development of B and T lymphocytes...
  12. ncbi request reprint The taming of a transposon: V(D)J recombination and the immune system
    Jessica M Jones
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington DC, USA
    Immunol Rev 200:233-48. 2004
    ..This work highlights the considerable diversity of transposition systems and their relation to V(D)J recombination...
  13. ncbi request reprint Tetramerization and DNA ligase IV interaction of the DNA double-strand break repair protein XRCC4 are mutually exclusive
    Mauro Modesti
    Department of Cell Biology and Genetics, Erasmus Medical Center, P O Box 1738, 3000 DR, Rotterdam, The Netherlands
    J Mol Biol 334:215-28. 2003
    ..We propose that the putative function of the XRCC4 tetramer is distinct from its DNA ligase IV-associated function...
  14. ncbi request reprint DNA repair: from molecular mechanism to human disease
    Errol C Friedberg
    Department of Pathology, University of Texas Southwestern, Medical Center at Dallas, 75390, USA
    DNA Repair (Amst) 5:986-96. 2006