Robert S Fischer

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Protoc 7:2056-66. 2012
  2. pmc Microscopy in 3D: a biologist's toolbox
    Robert S Fischer
    National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 21:682-91. 2011
  3. pmc Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis
    Kenneth A Myers
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 192:321-34. 2011
  4. doi request reprint Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
    Yicong Wu
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Biotechnol 31:1032-8. 2013
  5. pmc A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
    Dylan T Burnette
    National Institute of Child Health and Human Development and 2 National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Cell Biol 205:83-96. 2014
  6. pmc Instant super-resolution imaging in live cells and embryos via analog image processing
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Methods 10:1122-6. 2013
  7. pmc Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Methods 9:749-54. 2012
  8. pmc Local cortical tension by myosin II guides 3D endothelial cell branching
    Robert S Fischer
    Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 19:260-5. 2009

Collaborators

Detail Information

Publications8

  1. pmc Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Protoc 7:2056-66. 2012
    ..This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations...
  2. pmc Microscopy in 3D: a biologist's toolbox
    Robert S Fischer
    National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 21:682-91. 2011
    ..We discuss a few specific microscopy modalities that we believe will be particularly advantageous in imaging cells and subcellular structures in physiologically relevant 3D environments...
  3. pmc Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis
    Kenneth A Myers
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 192:321-34. 2011
    ..Thus, compliance and dimensionality ECM mechanosensing pathways independently regulate specific and distinct microtubule dynamics parameters in endothelial cells to guide branching morphogenesis in physically complex ECMs...
  4. doi request reprint Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
    Yicong Wu
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Biotechnol 31:1032-8. 2013
    ....
  5. pmc A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
    Dylan T Burnette
    National Institute of Child Health and Human Development and 2 National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Cell Biol 205:83-96. 2014
    ....
  6. pmc Instant super-resolution imaging in live cells and embryos via analog image processing
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Methods 10:1122-6. 2013
    ..We demonstrate the capability of our system by imaging fine, rapidly moving structures including motor-driven organelles in human lung fibroblasts and the cytoskeleton of flowing blood cells within developing zebrafish embryos. ..
  7. pmc Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Methods 9:749-54. 2012
    ..Our method uses commercially available parts and open-source software and is simpler than existing SIM implementations, allowing easy integration with wide-field microscopes...
  8. pmc Local cortical tension by myosin II guides 3D endothelial cell branching
    Robert S Fischer
    Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 19:260-5. 2009
    ..Thus, local downregulation of myosin II cortical contraction allows pseudopodium initiation to mediate EC branching and hence guide directional migration and angiogenesis...