Robert S Fischer
Affiliation: National Institutes of Health
- Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behaviorRobert S Fischer
Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
Nat Protoc 7:2056-66. 2012..This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations...
- Microscopy in 3D: a biologist's toolboxRobert S Fischer
National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Trends Cell Biol 21:682-91. 2011..We discuss a few specific microscopy modalities that we believe will be particularly advantageous in imaging cells and subcellular structures in physiologically relevant 3D environments...
- Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesisKenneth A Myers
Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 192:321-34. 2011..Thus, compliance and dimensionality ECM mechanosensing pathways independently regulate specific and distinct microtubule dynamics parameters in endothelial cells to guide branching morphogenesis in physically complex ECMs...
- Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopyAndrew G York
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health NIH, Bethesda, Maryland, USA
Nat Methods 9:749-54. 2012..Our method uses commercially available parts and open-source software and is simpler than existing SIM implementations, allowing easy integration with wide-field microscopes...
- Local cortical tension by myosin II guides 3D endothelial cell branchingRobert S Fischer
Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Curr Biol 19:260-5. 2009..Thus, local downregulation of myosin II cortical contraction allows pseudopodium initiation to mediate EC branching and hence guide directional migration and angiogenesis...