Research Topics
| Robert S FischerSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behaviorRobert S Fischer
Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
Nat Protoc 7:2056-66. 2012..This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations...
Microscopy in 3D: a biologist's toolboxRobert S Fischer
National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Trends Cell Biol 21:682-91. 2011..We discuss a few specific microscopy modalities that we believe will be particularly advantageous in imaging cells and subcellular structures in physiologically relevant 3D environments...
Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesisKenneth A Myers
Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 192:321-34. 2011..Thus, compliance and dimensionality ECM mechanosensing pathways independently regulate specific and distinct microtubule dynamics parameters in endothelial cells to guide branching morphogenesis in physically complex ECMs...
Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopyAndrew G York
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health NIH, Bethesda, Maryland, USA
Nat Methods 9:749-54. 2012..Our method uses commercially available parts and open-source software and is simpler than existing SIM implementations, allowing easy integration with wide-field microscopes...
Local cortical tension by myosin II guides 3D endothelial cell branchingRobert S Fischer
Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Curr Biol 19:260-5. 2009..Thus, local downregulation of myosin II cortical contraction allows pseudopodium initiation to mediate EC branching and hence guide directional migration and angiogenesis...
