Research Topics
Species | Michael Graham EspeySummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Selective metabolism of kynurenine in the spleen in the absence of indoleamine 2,3-dioxygenase inductionM G Espey
Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Immunol Lett 71:67-72. 2000..In vivo manipulation of Quin synthesis in the absence of IDO induction may serve as a model for studying regulation and function of the kynurenine pathway activation in the immune system...
Role of oxygen gradients in shaping redox relationships between the human intestine and its microbiotaMichael Graham Espey
Office of the Scientific Director, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 55:130-40. 2013..This review illustrates how the intestine and microbes utilize oxygen gradients as a backdrop for mechanistically shaping redox relationships and a functional coexistence...
Tumor macrophage redox and effector mechanisms associated with hypoxiaMichael Graham Espey
Radiation Biology Branch, National Cancer Institute, NIH, 10 B3 B69, 9000 Rockville Pike, Bethesda, MD 20892, USA
Free Radic Biol Med 41:1621-8. 2006..The relationships among innate immunity, redox systems, and the plasticity of phenotypic changes tumor-associated macrophages undergo in conjunction with tumor hypoxia will be examined...- Ingress and reactive chemistry of nitroxyl-derived species within human cellsMichael Graham Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD20892, USA
Free Radic Biol Med 33:827-34. 2002..The substantial facilitation of oxygen-dependent nitroxyl chemistry by intact lipid bilayers supports a focusing role for the membrane in modulation of cellular constituents proteins by this unique species... - Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxideMichael G Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:11127-32. 2002..These modifications may be favored in microenvironments where the rate of O2- production is temporally and spatially contemporaneous with nitric oxide synthase activity, but not in excess of NO generation... - A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide speciesMichael G Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Ann N Y Acad Sci 962:195-206. 2002..From this perspective of NO chemistry, the type, magnitude, location, and duration of either ROS or RNOS reactions may be predicted... - Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanismsMichael Graham Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:3481-6. 2002..Taken together, these findings exemplified that heme-peroxidase-catalyzed formation of NO(2) may play a pivotal role in inflammatory and chronic disease settings while calling into question the significance of nitration by peroxynitrite... - Mechanisms of cell death governed by the balance between nitrosative and oxidative stressM G Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Ann N Y Acad Sci 899:209-21. 2000..We here describe how the mechanisms of cell death are interwoven in the balance between the different chemical intermediates involved in nitrosative and oxidative stress... - Distinction between nitrosating mechanisms within human cells and aqueous solutionM G Espey
Radiation Biology Branch, Division of Clinical Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:30085-91. 2001..Although NO(2) was not a component of aqueous NO autoxidation, the results suggest that the intermediacy of this species may be a significant factor in the advent of either nitrosation or oxidation chemistry in biological systems... - The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signalingSonia Donzelli
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Antioxid Redox Signal 8:1363-71. 2006..In this review, we discuss the biochemistry of such reactions in the context of their pharmacologic and biologic implications... - Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reactionDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:12691-6. 2002..Metal catalyzed nitration may account for the observed specificity of protein nitration seen under pathological conditions, suggesting a major role for translocated metals and the labilization of heme in NT formation... - Pharmacologic doses of ascorbate act as a prooxidant and decrease growth of aggressive tumor xenografts in miceQi Chen
Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, and Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 105:11105-9. 2008..Similar pharmacologic concentrations were readily achieved in humans given ascorbate intravenously. These data suggest that ascorbate as a prodrug may have benefits in cancers with poor prognosis and limited therapeutic options... - Generation of nitroxyl by heme protein-mediated peroxidation of hydroxylamine but not N-hydroxy-L-arginineSonia Donzelli
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Free Radic Biol Med 45:578-84. 2008..That peroxidation of NH2OH by horseradish peroxidase produced free HNO, which was able to affect intracellular targets, was verified by conversion of 4,5-diaminofluorescein to the corresponding fluorophore within intact cells... - Discriminating formation of HNO from other reactive nitrogen oxide speciesSonia Donzelli
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 40:1056-66. 2006..These data indicate that free HNO can be biosynthesized and thus may function as an endogenous signaling agent that is regulated by GSH content... - Superoxide fluxes limit nitric oxide-induced signalingDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 281:25984-93. 2006..We conclude that the reaction of O2- with NO is an important regulatory mechanism, which modulates signaling pathways by limiting steady-state levels of NO and preventing H2O2 formation from O2-... - Heme proteins and nitric oxide (NO): the neglected, eloquent chemistry in NO redox signaling and regulationDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Antioxid Redox Signal 5:307-17. 2003..Here the basic chemistry of nitrosylation and the interactions of NO and other nitrogen oxides with metal-oxo species such as found in peroxidases and monoxygenases are discussed... - Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradientsJeff S Isenberg
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 40:1028-33. 2006..The findings suggest that subnanomolar NO gradients are sufficient to mobilize endothelial cell migration into hypoxic tissue during neovascularization events, such as in wound healing and cancer... - Guide for the use of nitric oxide (NO) donors as probes of the chemistry of NO and related redox species in biological systemsDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Institutes of Health/National Cancer Institute, Bethesda, Maryland 20892, USA
Methods Enzymol 359:84-105. 2002 - Vitamin C: a concentration-function approach yields pharmacology and therapeutic discoveriesMark Levine
Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1372, USA
Adv Nutr 2:78-88. 2011..The effects of pharmacologic ascorbate should be further studied in diseases, such as cancer and infections, which may respond to generation of reactive oxygen species via H(2)O(2)... - Hypoxic inducible factor 1alpha, extracellular signal-regulated kinase, and p53 are regulated by distinct threshold concentrations of nitric oxideDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 101:8894-9. 2004..The findings show that concentration and duration of NO exposure are critical determinants in the regulation of tumor-related proteins... - Ascorbate in pharmacologic concentrations selectively generates ascorbate radical and hydrogen peroxide in extracellular fluid in vivoQi Chen
Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 104:8749-54. 2007..These data provide a foundation for pursuing pharmacologic ascorbate as a prooxidant therapeutic agent in cancer and infections... - Pharmacologic ascorbate synergizes with gemcitabine in preclinical models of pancreatic cancerMichael Graham Espey
Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 50:1610-9. 2011..These data support the testing of pharmacologic ascorbate in adjunctive treatments for cancers prone to high failure rates with conventional therapeutic regimens, such as pancreatic cancer... - Vitamin C: intravenous use by complementary and alternative medicine practitioners and adverse effectsSebastian J Padayatty
Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 5:e11414. 2010..Anecdotal information and case reports suggest that intravenously administered vitamin C is used by Complementary and Alternate Medicine (CAM) practitioners. The scale of such use in the U.S. and associated side effects are unknown... - Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissuesQi Chen
Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:13604-9. 2005..These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H(2)O(2) may be beneficial... - The emerging biology of the nitrite anionMark T Gladwin
Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
Nat Chem Biol 1:308-14. 2005..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics... - Measurement of plasma volume using fluorescent silica-based nanoparticlesChristoph Eisner
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Appl Physiol 112:681-7. 2012..Collectively, the findings support a nanotechnology-based solution to methodological problems in measure of PV, notably in clinical settings where information on hemodynamic changes may improve treatment of injury and disease... - Nitric oxide in wound-healingJeff S Isenberg
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1500, USA
Microsurgery 25:442-51. 2005..Current research suggests that nitric oxide and several nitric oxide donors can exert biologic effects, although the particular net responses of cells contributing to wound repair are context-dependent... - Vitamin C transporter Slc23a1 links renal reabsorption, vitamin C tissue accumulation, and perinatal survival in miceChristopher P Corpe
Molecular and Clinical Nutrition Section, Digestive Diseases Branch, Intramural Research Program, National Institute of Diabetes and Digestive and Kidney Diseases NIDDK, NIH, Bethesda, Maryland, USA
J Clin Invest 120:1069-83. 2010..These findings indicate a key role for Slc23a1 in renal ascorbate absorption and perinatal survival and reveal regulation of vitamin C biosynthesis in mice... - Ascorbic acid kills Epstein-Barr virus positive Burkitt lymphoma cells and Epstein-Barr virus transformed B-cells in vitro, but not in vivoAmber N Shatzer
Medical Virology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA
Leuk Lymphoma 54:1069-78. 2013..Thus, while ascorbic acid was highly effective at killing EBV-positive BL cells and LCLs in vitro, it antagonized cell killing by bortezomib and was ineffective in an animal model... - Dynamic monitoring of localized tumor oxygenation changes using RF pulsed electron paramagnetic resonance in conscious miceShingo Matsumoto
Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1002, USA
Magn Reson Med 59:619-25. 2008..9 +/- 0.5 mmHg for tumor, 8.8 +/- 1.2 mmHg for muscle). These results support the advantage of pulsed EPR in examining pO2 in conscious animals with LiPc chronically implanted in predetermined regions... - Plasma membrane cholesterol content affects nitric oxide diffusion dynamics and signalingShane Miersch
Department of Chemistry and Biochemistry University of Windsor, Windsor Ontario N9B 3P4, Canada
J Biol Chem 283:18513-21. 2008..These findings suggest that the impact of cholesterol on membrane fluidity and microdomain structure contributes to the spatial heterogeneity of NO diffusion and signaling... - Inhibition of poly(ADP-RIBOSE) polymerase (PARP) by nitric oxide and reactive nitrogen oxide speciesOlga Sidorkina
Groupe Reparation de l ADN UMR 8113, LBPA ENS Cachan, Intitut Gustave Roussy, Villejuif Cedex, France
Free Radic Biol Med 35:1431-8. 2003....