Michael Graham Espey

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Selective metabolism of kynurenine in the spleen in the absence of indoleamine 2,3-dioxygenase induction
    M G Espey
    Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Immunol Lett 71:67-72. 2000
  2. ncbi request reprint A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide species
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 962:195-206. 2002
  3. doi request reprint Role of oxygen gradients in shaping redox relationships between the human intestine and its microbiota
    Michael Graham Espey
    Office of the Scientific Director, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 55:130-40. 2013
  4. pmc Tumor macrophage redox and effector mechanisms associated with hypoxia
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, NIH, 10 B3 B69, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:1621-8. 2006
  5. ncbi request reprint Ingress and reactive chemistry of nitroxyl-derived species within human cells
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD20892, USA
    Free Radic Biol Med 33:827-34. 2002
  6. pmc Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxide
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:11127-32. 2002
  7. pmc Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:3481-6. 2002
  8. ncbi request reprint Mechanisms of cell death governed by the balance between nitrosative and oxidative stress
    M G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 899:209-21. 2000
  9. ncbi request reprint Distinction between nitrosating mechanisms within human cells and aqueous solution
    M G Espey
    Radiation Biology Branch, Division of Clinical Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:30085-91. 2001
  10. ncbi request reprint The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signaling
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1363-71. 2006

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Selective metabolism of kynurenine in the spleen in the absence of indoleamine 2,3-dioxygenase induction
    M G Espey
    Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Immunol Lett 71:67-72. 2000
    ..In vivo manipulation of Quin synthesis in the absence of IDO induction may serve as a model for studying regulation and function of the kynurenine pathway activation in the immune system...
  2. ncbi request reprint A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide species
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 962:195-206. 2002
    ..From this perspective of NO chemistry, the type, magnitude, location, and duration of either ROS or RNOS reactions may be predicted...
  3. doi request reprint Role of oxygen gradients in shaping redox relationships between the human intestine and its microbiota
    Michael Graham Espey
    Office of the Scientific Director, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 55:130-40. 2013
    ..This review illustrates how the intestine and microbes utilize oxygen gradients as a backdrop for mechanistically shaping redox relationships and a functional coexistence...
  4. pmc Tumor macrophage redox and effector mechanisms associated with hypoxia
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, NIH, 10 B3 B69, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:1621-8. 2006
    ..The relationships among innate immunity, redox systems, and the plasticity of phenotypic changes tumor-associated macrophages undergo in conjunction with tumor hypoxia will be examined...
  5. ncbi request reprint Ingress and reactive chemistry of nitroxyl-derived species within human cells
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD20892, USA
    Free Radic Biol Med 33:827-34. 2002
    ..The substantial facilitation of oxygen-dependent nitroxyl chemistry by intact lipid bilayers supports a focusing role for the membrane in modulation of cellular constituents proteins by this unique species...
  6. pmc Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxide
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:11127-32. 2002
    ..These modifications may be favored in microenvironments where the rate of O2- production is temporally and spatially contemporaneous with nitric oxide synthase activity, but not in excess of NO generation...
  7. pmc Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:3481-6. 2002
    ..Taken together, these findings exemplified that heme-peroxidase-catalyzed formation of NO(2) may play a pivotal role in inflammatory and chronic disease settings while calling into question the significance of nitration by peroxynitrite...
  8. ncbi request reprint Mechanisms of cell death governed by the balance between nitrosative and oxidative stress
    M G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 899:209-21. 2000
    ..We here describe how the mechanisms of cell death are interwoven in the balance between the different chemical intermediates involved in nitrosative and oxidative stress...
  9. ncbi request reprint Distinction between nitrosating mechanisms within human cells and aqueous solution
    M G Espey
    Radiation Biology Branch, Division of Clinical Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:30085-91. 2001
    ..Although NO(2) was not a component of aqueous NO autoxidation, the results suggest that the intermediacy of this species may be a significant factor in the advent of either nitrosation or oxidation chemistry in biological systems...
  10. ncbi request reprint The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signaling
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1363-71. 2006
    ..In this review, we discuss the biochemistry of such reactions in the context of their pharmacologic and biologic implications...
  11. ncbi request reprint Superoxide fluxes limit nitric oxide-induced signaling
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:25984-93. 2006
    ..We conclude that the reaction of O2- with NO is an important regulatory mechanism, which modulates signaling pathways by limiting steady-state levels of NO and preventing H2O2 formation from O2-...
  12. pmc Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reaction
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:12691-6. 2002
    ..Metal catalyzed nitration may account for the observed specificity of protein nitration seen under pathological conditions, suggesting a major role for translocated metals and the labilization of heme in NT formation...
  13. pmc Pharmacologic doses of ascorbate act as a prooxidant and decrease growth of aggressive tumor xenografts in mice
    Qi Chen
    Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, and Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:11105-9. 2008
    ..Similar pharmacologic concentrations were readily achieved in humans given ascorbate intravenously. These data suggest that ascorbate as a prodrug may have benefits in cancers with poor prognosis and limited therapeutic options...
  14. pmc Generation of nitroxyl by heme protein-mediated peroxidation of hydroxylamine but not N-hydroxy-L-arginine
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Free Radic Biol Med 45:578-84. 2008
    ..That peroxidation of NH2OH by horseradish peroxidase produced free HNO, which was able to affect intracellular targets, was verified by conversion of 4,5-diaminofluorescein to the corresponding fluorophore within intact cells...
  15. ncbi request reprint Discriminating formation of HNO from other reactive nitrogen oxide species
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 40:1056-66. 2006
    ..These data indicate that free HNO can be biosynthesized and thus may function as an endogenous signaling agent that is regulated by GSH content...
  16. ncbi request reprint Heme proteins and nitric oxide (NO): the neglected, eloquent chemistry in NO redox signaling and regulation
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 5:307-17. 2003
    ..Here the basic chemistry of nitrosylation and the interactions of NO and other nitrogen oxides with metal-oxo species such as found in peroxidases and monoxygenases are discussed...
  17. pmc Ascorbate in pharmacologic concentrations selectively generates ascorbate radical and hydrogen peroxide in extracellular fluid in vivo
    Qi Chen
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:8749-54. 2007
    ..These data provide a foundation for pursuing pharmacologic ascorbate as a prooxidant therapeutic agent in cancer and infections...
  18. ncbi request reprint Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradients
    Jeff S Isenberg
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 40:1028-33. 2006
    ..The findings suggest that subnanomolar NO gradients are sufficient to mobilize endothelial cell migration into hypoxic tissue during neovascularization events, such as in wound healing and cancer...
  19. ncbi request reprint Guide for the use of nitric oxide (NO) donors as probes of the chemistry of NO and related redox species in biological systems
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health National Cancer Institute, Bethesda, Maryland 20892, USA
    Methods Enzymol 359:84-105. 2002
  20. pmc Vitamin C: a concentration-function approach yields pharmacology and therapeutic discoveries
    Mark Levine
    Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1372, USA
    Adv Nutr 2:78-88. 2011
    ..The effects of pharmacologic ascorbate should be further studied in diseases, such as cancer and infections, which may respond to generation of reactive oxygen species via H(2)O(2)...
  21. pmc Hypoxic inducible factor 1alpha, extracellular signal-regulated kinase, and p53 are regulated by distinct threshold concentrations of nitric oxide
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:8894-9. 2004
    ..The findings show that concentration and duration of NO exposure are critical determinants in the regulation of tumor-related proteins...
  22. pmc Pharmacologic ascorbate synergizes with gemcitabine in preclinical models of pancreatic cancer
    Michael Graham Espey
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 50:1610-9. 2011
    ..These data support the testing of pharmacologic ascorbate in adjunctive treatments for cancers prone to high failure rates with conventional therapeutic regimens, such as pancreatic cancer...
  23. doi request reprint Ascorbic acid kills Epstein-Barr virus positive Burkitt lymphoma cells and Epstein-Barr virus transformed B-cells in vitro, but not in vivo
    Amber N Shatzer
    Medical Virology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Leuk Lymphoma 54:1069-78. 2013
    ..Thus, while ascorbic acid was highly effective at killing EBV-positive BL cells and LCLs in vitro, it antagonized cell killing by bortezomib and was ineffective in an animal model...
  24. pmc Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues
    Qi Chen
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:13604-9. 2005
    ..These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H(2)O(2) may be beneficial...
  25. pmc Vitamin C: intravenous use by complementary and alternative medicine practitioners and adverse effects
    Sebastian J Padayatty
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 5:e11414. 2010
    ..Anecdotal information and case reports suggest that intravenously administered vitamin C is used by Complementary and Alternate Medicine (CAM) practitioners. The scale of such use in the U.S. and associated side effects are unknown...
  26. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...
  27. pmc Measurement of plasma volume using fluorescent silica-based nanoparticles
    Christoph Eisner
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Appl Physiol (1985) 112:681-7. 2012
    ..Collectively, the findings support a nanotechnology-based solution to methodological problems in measure of PV, notably in clinical settings where information on hemodynamic changes may improve treatment of injury and disease...
  28. ncbi request reprint Nitric oxide in wound-healing
    Jeff S Isenberg
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1500, USA
    Microsurgery 25:442-51. 2005
    ..Current research suggests that nitric oxide and several nitric oxide donors can exert biologic effects, although the particular net responses of cells contributing to wound repair are context-dependent...
  29. pmc Vitamin C transporter Slc23a1 links renal reabsorption, vitamin C tissue accumulation, and perinatal survival in mice
    Christopher P Corpe
    Molecular and Clinical Nutrition Section, Digestive Diseases Branch, Intramural Research Program, National Institute of Diabetes and Digestive and Kidney Diseases NIDDK, NIH, Bethesda, Maryland, USA
    J Clin Invest 120:1069-83. 2010
    ..These findings indicate a key role for Slc23a1 in renal ascorbate absorption and perinatal survival and reveal regulation of vitamin C biosynthesis in mice...
  30. doi request reprint Dynamic monitoring of localized tumor oxygenation changes using RF pulsed electron paramagnetic resonance in conscious mice
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 59:619-25. 2008
    ..9 +/- 0.5 mmHg for tumor, 8.8 +/- 1.2 mmHg for muscle). These results support the advantage of pulsed EPR in examining pO2 in conscious animals with LiPc chronically implanted in predetermined regions...
  31. pmc Plasma membrane cholesterol content affects nitric oxide diffusion dynamics and signaling
    Shane Miersch
    Department of Chemistry and Biochemistry University of Windsor, Windsor Ontario N9B 3P4, Canada
    J Biol Chem 283:18513-21. 2008
    ..These findings suggest that the impact of cholesterol on membrane fluidity and microdomain structure contributes to the spatial heterogeneity of NO diffusion and signaling...
  32. ncbi request reprint Inhibition of poly(ADP-RIBOSE) polymerase (PARP) by nitric oxide and reactive nitrogen oxide species
    Olga Sidorkina
    Groupe Reparation de l ADN UMR 8113, LBPA ENS Cachan, Intitut Gustave Roussy, Villejuif Cedex, France
    Free Radic Biol Med 35:1431-8. 2003
    ....