John T Elliott

Summary

Affiliation: National Institute of Standards and Technology
Country: USA

Publications

  1. pmc Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro
    Aonghus J O'Loughlin
    Dept of Obstetrics and Gynaecology, National University of Ireland Galway, Clinical Science Institute, University College Hospital Galway, Newcastle Road, Galway, Ireland
    Reprod Biol Endocrinol 3:8. 2005
  2. pmc Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy
    Kiran Bhadriraju
    SAIC, Arlington, VA, USA
    BMC Cell Biol 8:43. 2007
  3. ncbi request reprint The effect of surface chemistry on the formation of thin films of native fibrillar collagen
    John T Elliott
    Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Biomaterials 28:576-85. 2007
  4. ncbi request reprint Evaluating the performance of fibrillar collagen films formed at polystyrene surfaces as cell culture substrates
    John T Elliott
    Cell and Tissue Measurements Group, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biointerphases 3:19. 2008
  5. ncbi request reprint Gradient substrate assembly for quantifying cellular response to biomaterials
    Ying Mei
    Polymers Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    J Biomed Mater Res A 79:974-88. 2006
  6. doi request reprint Cell cycle dependent TN-C promoter activity determined by live cell imaging
    Michael Halter
    Cell Systems Science Group Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 79:192-202. 2011
  7. ncbi request reprint Tools for quantitative and validated measurements of cells
    Anne L Plant
    National Institute of Standards and Technology, Gaithersburg, MD, USA
    Methods Mol Biol 356:95-107. 2007
  8. ncbi request reprint Automated live cell imaging of green fluorescent protein degradation in individual fibroblasts
    Michael Halter
    Cell and Tissue Measurements Group, Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 71:827-34. 2007
  9. doi request reprint A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
    Michael Halter
    Cell Systems Science Group Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Assay Drug Dev Technol 7:356-65. 2009
  10. pmc Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity
    Kurt J Langenbach
    Biotechnology Division National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    BMC Biotechnol 6:14. 2006

Collaborators

Detail Information

Publications22

  1. pmc Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro
    Aonghus J O'Loughlin
    Dept of Obstetrics and Gynaecology, National University of Ireland Galway, Clinical Science Institute, University College Hospital Galway, Newcastle Road, Galway, Ireland
    Reprod Biol Endocrinol 3:8. 2005
    ..The aims of this study were to evaluate the effects of thrombin, a specific PAR-1 activating peptide (PAR1-AP), and a PAR-1 antagonist on human umbilical artery tone in vitro...
  2. pmc Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy
    Kiran Bhadriraju
    SAIC, Arlington, VA, USA
    BMC Cell Biol 8:43. 2007
    ..This method allows us to concurrently examine cell morphology, cell-cell contact, and myosin light chain diphosphorylation in vascular smooth muscle cells...
  3. ncbi request reprint The effect of surface chemistry on the formation of thin films of native fibrillar collagen
    John T Elliott
    Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Biomaterials 28:576-85. 2007
    ..Morphology studies with vascular smooth muscle cells indicated that only collagen films formed on hydrophobic substrates mimicked the biological properties of fibrillar collagen gels...
  4. ncbi request reprint Evaluating the performance of fibrillar collagen films formed at polystyrene surfaces as cell culture substrates
    John T Elliott
    Cell and Tissue Measurements Group, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biointerphases 3:19. 2008
    ..The results of this study suggest that biologically relevant, robust thin films of collagen fibrils can be formed in any laboratory in untreated polystyrene dishes and multi-well polystyrene plates...
  5. ncbi request reprint Gradient substrate assembly for quantifying cellular response to biomaterials
    Ying Mei
    Polymers Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    J Biomed Mater Res A 79:974-88. 2006
    ..The results demonstrate the role of gradient substrate assembly as a method for quantifying the relationship between protein and cellular response to technologically relevant polymeric materials...
  6. doi request reprint Cell cycle dependent TN-C promoter activity determined by live cell imaging
    Michael Halter
    Cell Systems Science Group Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 79:192-202. 2011
    ....
  7. ncbi request reprint Tools for quantitative and validated measurements of cells
    Anne L Plant
    National Institute of Standards and Technology, Gaithersburg, MD, USA
    Methods Mol Biol 356:95-107. 2007
    ..The inherent distribution of responses in a cell population will determine how many cells must be measured to reach an accurate determination of cellular response...
  8. ncbi request reprint Automated live cell imaging of green fluorescent protein degradation in individual fibroblasts
    Michael Halter
    Cell and Tissue Measurements Group, Biochemical Science Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Cytometry A 71:827-34. 2007
    ..The approach described in this study will assist the quantification and understanding of gene activity within live cells using fluorescent protein reporters...
  9. doi request reprint A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
    Michael Halter
    Cell Systems Science Group Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Assay Drug Dev Technol 7:356-65. 2009
    ..Unlike the other assays, monitoring cellular GFP on a per-cell basis allows detection of reduced ribosome activity before significant cell death occurs, and the results are not convoluted by the numbers of cells being assayed...
  10. pmc Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity
    Kurt J Langenbach
    Biotechnology Division National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    BMC Biotechnol 6:14. 2006
    ..We have quantitatively examined the relationship between fibroblast morphology and activation of the promoter for the extracellular matrix protein tenascin-C using a tenascin-C promoter-based GFP reporter construct...
  11. ncbi request reprint Vascular smooth muscle cell response on thin films of collagen
    John T Elliott
    Biotechnology Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Matrix Biol 24:489-502. 2005
    ..These thin films will be useful for elucidating the features of the collagen matrix that regulate vSMC response and may be applicable to high content screening...
  12. doi request reprint The relative roles of collagen adhesive receptor DDR2 activation and matrix stiffness on the downregulation of focal adhesion kinase in vascular smooth muscle cells
    Kiran Bhadriraju
    SAIC, Mail Stop 8313, 100 Bureau Drive, Gaithersburg, MD 20899, USA
    Biomaterials 30:6687-94. 2009
    ..Our results suggest that the collagen receptor DDR2 is involved in the regulation of FAK levels in vSMC adhered to Type I collagen matrices, and that regulation of FAK levels in these cells appears to be independent of matrix stiffness...
  13. doi request reprint Cell response to matrix mechanics: focus on collagen
    Anne L Plant
    National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, USA
    Biochim Biophys Acta 1793:893-902. 2009
    ..The temporal response of cells to differences in ECM may provide insight into mechanisms of signal transduction...
  14. pmc The stiffness of collagen fibrils influences vascular smooth muscle cell phenotype
    Dennis P McDaniel
    Biochemical Science Division and Manufacturing Metrology Division National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biophys J 92:1759-69. 2007
    ..We suggest that increase in the nanoscale rigidity of collagen fibrils can cause these cells to assume a proliferative phenotype...
  15. doi request reprint Reproducibility and robustness of a real-time microfluidic cell toxicity assay
    Gregory A Cooksey
    Biochemical Science Division, NIST, Gaithersburg, Maryland, USA
    Anal Chem 83:3890-6. 2011
    ..Both microfluidic and larger scale assay conditions showed comparable GFP decay rates upon CHX exposure, but the microfluidic data provided the higher level of confidence...
  16. doi request reprint Cell volume distributions reveal cell growth rates and division times
    Michael Halter
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    J Theor Biol 257:124-30. 2009
    ..The derivation and application of the model allows one to relate the stationary population distribution of cell volumes to extrinsic biological noise present in growing and dividing cell cultures...
  17. ncbi request reprint Tuning cell adhesion on gradient poly(2-hydroxyethyl methacrylate)-grafted surfaces
    Ying Mei
    Polymers Division and Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Langmuir 21:12309-14. 2005
    ....
  18. doi request reprint Hybrid cell adhesive material for instant dielectrophoretic cell trapping and long-term cell function assessment
    Darwin R Reyes
    Semiconductor Electronics Division, Physical Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, MS 8120, Gaithersburg, Maryland 20899 8120, United States
    Langmuir 27:10027-34. 2011
    ..This approach could have further applications in areas such as cell-cell communication, three-dimensional cell aggregates to create cell microenvironments, and cell cocultures...
  19. pmc New concepts for building vocabulary for cell image ontologies
    Anne L Plant
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    BMC Bioinformatics 12:487. 2011
    ....
  20. doi request reprint Comparison of segmentation algorithms for fluorescence microscopy images of cells
    Alden A Dima
    Software and Systems Division, Information Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Cytometry A 79:545-59. 2011
    ..The results of this study will assist the development of criteria for evaluating interlaboratory comparability...
  21. ncbi request reprint Comparison of reagents for shape analysis of fixed cells by automated fluorescence microscopy
    John T Elliott
    Biomolecular Materials Group, Biotechnology Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Cytometry A 52:90-100. 2003
    ..Generating adequate contrast at the edge of thin well-spread cells can be challenging...
  22. ncbi request reprint Biocompatibility of sorbitol-containing polyesters. Part I: Synthesis, surface analysis and cell response in vitro
    Ying Mei
    Department of Chemistry and Chemical Engineering, NSF I UCRC Center for Biocatalysis and Bioprocessing of Macromolecules, Polytechnic University, Six Metrotech Center, Brooklyn, NY 11201, USA
    Biomaterials 25:4195-201. 2004
    ..These results establish the sorbitol-containing polyester series as a promising material for tissue engineering research and development...