C E Dunbar

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint The use of nonhuman primate models to improve gene transfer into haematopoietic stem cells
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville Pike, Bethesda, MD, USA
    J Intern Med 249:329-38. 2001
  2. pmc Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony-stimulating factor
    C E Dunbar
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:11871-6. 1996
  3. ncbi request reprint Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating ability
    J F Tisdale
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Blood 92:1131-41. 1998
  4. ncbi request reprint The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate model
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 938:236-44; discussion 244-5. 2001
  5. pmc Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands
    C Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Oral Dis 16:269-77. 2010
  6. pmc Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates
    M Takatoku
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 108:447-55. 2001
  7. ncbi request reprint The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells
    S E Sellers
    Hematology Branch, The National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    Blood 97:1888-91. 2001
  8. ncbi request reprint In vivo persistence of retrovirally transduced murine long-term repopulating cells is not limited by expression of foreign gene products in the fully or minimally myeloablated setting
    E Kang
    Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Disorders/NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Hum Gene Ther 12:1663-72. 2001
  9. ncbi request reprint Assessment of rapid remobilization intervals with G-CSF and SCF in murine and rhesus macaque models
    P A Shi
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Transfusion 41:1438-44. 2001
  10. ncbi request reprint Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Hum Gene Ther 12:607-17. 2001

Collaborators

Detail Information

Publications20

  1. ncbi request reprint The use of nonhuman primate models to improve gene transfer into haematopoietic stem cells
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville Pike, Bethesda, MD, USA
    J Intern Med 249:329-38. 2001
    ..These and other advances should result in successful gene therapy for a variety of acquired and congenital disorders affecting HSCs and their progeny lineages...
  2. pmc Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony-stimulating factor
    C E Dunbar
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:11871-6. 1996
    ....
  3. ncbi request reprint Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating ability
    J F Tisdale
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Blood 92:1131-41. 1998
    ..However, a brief transduction in the presence of specific cytokines and stromal support allows engraftment with an encouraging number of retrovirally modified cells...
  4. ncbi request reprint The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate model
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 938:236-44; discussion 244-5. 2001
    ..This approach may allow more efficient engraftment of successfully transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion...
  5. pmc Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands
    C Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Oral Dis 16:269-77. 2010
    ..This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands...
  6. pmc Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates
    M Takatoku
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 108:447-55. 2001
    ..We observed stable in vivo overall gene marking levels of up to 29%. This approach may allow more efficient engraftment of transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion...
  7. ncbi request reprint The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells
    S E Sellers
    Hematology Branch, The National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    Blood 97:1888-91. 2001
    ..There was no evidence for expansion of MDR1 vector-transduced cells. Long-term engraftment with MDR1 vector- and neo vector-transduced cells occurred despite prolonged culture...
  8. ncbi request reprint In vivo persistence of retrovirally transduced murine long-term repopulating cells is not limited by expression of foreign gene products in the fully or minimally myeloablated setting
    E Kang
    Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Disorders/NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Hum Gene Ther 12:1663-72. 2001
    ..We conclude that even with very low dose conditioning, engraftment by genetically modified LTRC cells at clinically significant levels can be achieved without evidence for clearance of cells known to be expressing immunogenic proteins...
  9. ncbi request reprint Assessment of rapid remobilization intervals with G-CSF and SCF in murine and rhesus macaque models
    P A Shi
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Transfusion 41:1438-44. 2001
    ..Defining the optimum regimen and time for repeat peripheral blood progenitor cell mobilization would have important clinical applications...
  10. ncbi request reprint Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Hum Gene Ther 12:607-17. 2001
    ..Judicious application of these models should continue to be a priority, and advances should now be tested in proof-of-concept clinical trials...
  11. pmc AAV5-mediated gene transfer to the parotid glands of non-human primates
    A Voutetakis
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Gene Ther 17:50-60. 2010
    ..The aggregate data indicate that results with AAV5 vectors in murine salivary glands apparently do not extend to macaque glands...
  12. pmc Feline leukemia virus integrase and capsid packaging functions do not change the insertion profile of standard Moloney retroviral vectors
    J Y Métais
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene Ther 17:799-804. 2010
    ..However, considering the stability and efficacy of CatPac vectors, further development is warranted, using potentially safer vector backbones, for instance those with a SIN configuration...
  13. ncbi request reprint Analysis of origin and optimization of expansion and transduction of circulating peripheral blood endothelial progenitor cells in the rhesus macaque model
    J Hu
    Hematology Branch, National Heart, Lung, and Blood Institute NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Hum Gene Ther 13:2041-50. 2002
    ..Further elucidation of the origin and in vivo behavior of EPCs may be possible, using optimized transduction conditions and a vascular injury model...
  14. ncbi request reprint High-dose cyclophosphamide in severe aplastic anaemia: a randomised trial
    J F Tisdale
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Lancet 356:1554-9. 2000
    ....
  15. doi request reprint Active thrombopoiesis is associated with worse severity and activity of chronic GVHD
    T Bat
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Bone Marrow Transplant 48:1569-73. 2013
    ..Among patients with stable moderate or severe cGVHD, there was no evidence of hypoproduction of platelets. Future studies should further investigate the role of thrombopoiesis in cGVHD. ..
  16. ncbi request reprint Stem and progenitor cell therapies: insights from non-human primate models
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland, USA
    Cytotherapy 6:586-8. 2004
    ....
  17. ncbi request reprint BM-derived cell therapies for cardiovascular disease
    R O Cannon
    Cardiology Branch and Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytotherapy 9:305-15. 2007
  18. pmc T cell receptor VB repertoire diversity in patients with immune thrombocytopenia following splenectomy
    P F Fogarty
    Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1652, USA
    Clin Exp Immunol 133:461-6. 2003
    ..These findings suggest that removal of the spleen may lead directly or indirectly to reductions in T cell clonal expansions in responders, or that the extent of T cell clonality impacts responsiveness to splenectomy in patients with ITP...
  19. ncbi request reprint Rabaptin-5 is a novel fusion partner to platelet-derived growth factor beta receptor in chronic myelomonocytic leukemia
    M K Magnusson
    Hematology Branch, National Heart, Lung, and Blood Institute, and Laboratory of Pathology, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 98:2518-25. 2001
    ..Early endosomal transport is critical in regulation of various growth factor receptors, through ligand-induced clathrin-mediated endocytosis, and thus this new fusion protein links together 2 important pathways of growth regulation...
  20. ncbi request reprint Transplant dose of CD34(+) and CD3(+) cells predicts outcome in patients with haematological malignancies undergoing T cell-depleted peripheral blood stem cell transplants with delayed donor lymphocyte add-back
    R Nakamura
    Stem Cell Transplantation Unit, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Br J Haematol 115:95-104. 2001
    ..83 x 10(5) CD3(+) cells/kg. These results further define the impact of CD34 and CD3 cell dose on transplant outcome and show that careful dosing of stem cells and lymphocytes may permit the control and optimization of transplant outcome...