Research Topics
| C E DunbarSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
The use of nonhuman primate models to improve gene transfer into haematopoietic stem cellsC E Dunbar
Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Rockville Pike, Bethesda, MD, USA
J Intern Med 249:329-38. 2001..These and other advances should result in successful gene therapy for a variety of acquired and congenital disorders affecting HSCs and their progeny lineages...
Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony-stimulating factorC E Dunbar
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 93:11871-6. 1996....- Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating abilityJ F Tisdale
Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
Blood 92:1131-41. 1998..However, a brief transduction in the presence of specific cytokines and stromal support allows engraftment with an encouraging number of retrovirally modified cells... - The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate modelC E Dunbar
Molecular Hematopoiesis Section, Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA
Ann N Y Acad Sci 938:236-44; discussion 244-5. 2001..This approach may allow more efficient engraftment of successfully transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion... - Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glandsC Zheng
Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
Oral Dis 16:269-77. 2010..This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands... - Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primatesM Takatoku
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Clin Invest 108:447-55. 2001..We observed stable in vivo overall gene marking levels of up to 29%. This approach may allow more efficient engraftment of transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion... - The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cellsS E Sellers
Hematology Branch, The National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
Blood 97:1888-91. 2001..There was no evidence for expansion of MDR1 vector-transduced cells. Long-term engraftment with MDR1 vector- and neo vector-transduced cells occurred despite prolonged culture... - In vivo persistence of retrovirally transduced murine long-term repopulating cells is not limited by expression of foreign gene products in the fully or minimally myeloablated settingE Kang
Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Disorders/NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
Hum Gene Ther 12:1663-72. 2001..We conclude that even with very low dose conditioning, engraftment by genetically modified LTRC cells at clinically significant levels can be achieved without evidence for clearance of cells known to be expressing immunogenic proteins... - Assessment of rapid remobilization intervals with G-CSF and SCF in murine and rhesus macaque modelsP A Shi
Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
Transfusion 41:1438-44. 2001..Defining the optimum regimen and time for repeat peripheral blood progenitor cell mobilization would have important clinical applications... - Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cellsR E Donahue
Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
Hum Gene Ther 12:607-17. 2001..Judicious application of these models should continue to be a priority, and advances should now be tested in proof-of-concept clinical trials... - AAV5-mediated gene transfer to the parotid glands of non-human primatesA Voutetakis
Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
Gene Ther 17:50-60. 2010..The aggregate data indicate that results with AAV5 vectors in murine salivary glands apparently do not extend to macaque glands... - Feline leukemia virus integrase and capsid packaging functions do not change the insertion profile of standard Moloney retroviral vectorsJ Y Métais
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Gene Ther 17:799-804. 2010..However, considering the stability and efficacy of CatPac vectors, further development is warranted, using potentially safer vector backbones, for instance those with a SIN configuration... - Analysis of origin and optimization of expansion and transduction of circulating peripheral blood endothelial progenitor cells in the rhesus macaque modelJ Hu
Hematology Branch, National Heart, Lung, and Blood Institute/NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
Hum Gene Ther 13:2041-50. 2002..Further elucidation of the origin and in vivo behavior of EPCs may be possible, using optimized transduction conditions and a vascular injury model... - High-dose cyclophosphamide in severe aplastic anaemia: a randomised trialJ F Tisdale
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Lancet 356:1554-9. 2000.... - Stem and progenitor cell therapies: insights from non-human primate modelsC E Dunbar
Molecular Hematopoiesis Section, Hematology Branch National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland, USA
Cytotherapy 6:586-8. 2004.... - BM-derived cell therapies for cardiovascular diseaseR O Cannon
Cardiology Branch and Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Cytotherapy 9:305-15. 2007 - T cell receptor VB repertoire diversity in patients with immune thrombocytopenia following splenectomyP F Fogarty
Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1652, USA
Clin Exp Immunol 133:461-6. 2003..These findings suggest that removal of the spleen may lead directly or indirectly to reductions in T cell clonal expansions in responders, or that the extent of T cell clonality impacts responsiveness to splenectomy in patients with ITP... - Transplant dose of CD34(+) and CD3(+) cells predicts outcome in patients with haematological malignancies undergoing T cell-depleted peripheral blood stem cell transplants with delayed donor lymphocyte add-backR Nakamura
Stem Cell Transplantation Unit, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Br J Haematol 115:95-104. 2001..83 x 10(5) CD3(+) cells/kg. These results further define the impact of CD34 and CD3 cell dose on transplant outcome and show that careful dosing of stem cells and lymphocytes may permit the control and optimization of transplant outcome... - Rabaptin-5 is a novel fusion partner to platelet-derived growth factor beta receptor in chronic myelomonocytic leukemiaM K Magnusson
Hematology Branch, National Heart, Lung, and Blood Institute, and Laboratory of Pathology, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
Blood 98:2518-25. 2001..Early endosomal transport is critical in regulation of various growth factor receptors, through ligand-induced clathrin-mediated endocytosis, and thus this new fusion protein links together 2 important pathways of growth regulation...