Daniel C Douek

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Mapping of positive selection sites in the HIV-1 genome in the context of RNA and protein structural constraints
    Joke Snoeck
    Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland
    Retrovirology 8:87. 2011
  2. pmc Universal and specific quantitative detection of botulinum neurotoxin genes
    Brenna J Hill
    Human Immunology Section, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Microbiol 10:267. 2010
  3. ncbi request reprint T cell dynamics in HIV-1 infection
    Daniel C Douek
    Human Immunology Section Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Annu Rev Immunol 21:265-304. 2003
  4. ncbi request reprint Disrupting T-cell homeostasis: how HIV-1 infection causes disease
    Daniel C Douek
    Human Immunology Section, Vaccine Research Center, NIAID, NIH, Room 3509, 40 Convent Drive, Bethesda, MD 20892, USA
    AIDS Rev 5:172-7. 2003
  5. ncbi request reprint HIV preferentially infects HIV-specific CD4+ T cells
    Daniel C Douek
    Vaccine Research Center, NIAID, NIH, Maryland 20892, USA
    Nature 417:95-8. 2002
  6. ncbi request reprint A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape
    Daniel C Douek
    Department of Experimental Transplantation and Immunology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 168:3099-104. 2002
  7. pmc Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation
    Joseph P Casazza
    Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:2865-77. 2006
  8. pmc Graft-versus-leukemia effects associated with detectable Wilms tumor-1 specific T lymphocytes after allogeneic stem-cell transplantation for acute lymphoblastic leukemia
    Katayoun Rezvani
    Stem Cell Allotransplantation Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1201, USA
    Blood 110:1924-32. 2007
  9. ncbi request reprint The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration
    Michael R Betts
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6407-17. 2004
  10. pmc SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, 40 Convent Drive, Bethesda, MD 20892, USA
    Blood 110:928-36. 2007

Detail Information

Publications106 found, 100 shown here

  1. pmc Mapping of positive selection sites in the HIV-1 genome in the context of RNA and protein structural constraints
    Joke Snoeck
    Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland
    Retrovirology 8:87. 2011
    ....
  2. pmc Universal and specific quantitative detection of botulinum neurotoxin genes
    Brenna J Hill
    Human Immunology Section, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Microbiol 10:267. 2010
    ..botulinum regardless of the neurotoxin type. The second step uses quantitative PCR (qPCR) technology to determine the specific serotype of the neurotoxin...
  3. ncbi request reprint T cell dynamics in HIV-1 infection
    Daniel C Douek
    Human Immunology Section Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Annu Rev Immunol 21:265-304. 2003
    ....
  4. ncbi request reprint Disrupting T-cell homeostasis: how HIV-1 infection causes disease
    Daniel C Douek
    Human Immunology Section, Vaccine Research Center, NIAID, NIH, Room 3509, 40 Convent Drive, Bethesda, MD 20892, USA
    AIDS Rev 5:172-7. 2003
    ....
  5. ncbi request reprint HIV preferentially infects HIV-specific CD4+ T cells
    Daniel C Douek
    Vaccine Research Center, NIAID, NIH, Maryland 20892, USA
    Nature 417:95-8. 2002
    ..Furthermore, the phenomenon of HIV specifically infecting the very cells that respond to it adds a cautionary note to the practice of structured therapy interruption...
  6. ncbi request reprint A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape
    Daniel C Douek
    Department of Experimental Transplantation and Immunology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 168:3099-104. 2002
    ..Thus, CD8(+) T cells comprising multiple TCR clonotypes may expand in vivo in response to individual epitopes, and may increase the ability of the response to recognize virus escape mutants...
  7. pmc Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation
    Joseph P Casazza
    Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:2865-77. 2006
    ..Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes...
  8. pmc Graft-versus-leukemia effects associated with detectable Wilms tumor-1 specific T lymphocytes after allogeneic stem-cell transplantation for acute lymphoblastic leukemia
    Katayoun Rezvani
    Stem Cell Allotransplantation Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1201, USA
    Blood 110:1924-32. 2007
    ..Our results support the immunogenicity of WT1 after SCT for ALL and highlight the potential for WT1 vaccines to boost GVL after SCT for ALL...
  9. ncbi request reprint The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration
    Michael R Betts
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6407-17. 2004
    ..The inherent ability of viruses to induce high or low Ag states may be the primary determinant of the cytokine vs cytolytic nature of the virus-specific CD8(+) T cell response...
  10. pmc SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, 40 Convent Drive, Bethesda, MD 20892, USA
    Blood 110:928-36. 2007
    ..Manipulation of the interaction of PD-1 with its ligands could thus potentially restore the CD8+ T-cell responses in SIV infection...
  11. pmc The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donor
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 114:5071-80. 2009
    ....
  12. pmc Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infection
    David A Price
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:923-36. 2009
    ..Thus, the pattern of antigen-specific clonotype recruitment within a protective CD8(+) T cell population is a prognostic indicator of vaccine efficacy and biological outcome in an AIDS virus infection...
  13. pmc Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
    J Immunol 183:1120-32. 2009
    ..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection...
  14. pmc Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
    David A Price
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 202:1349-61. 2005
    ..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function...
  15. pmc High production rates sustain in vivo levels of PD-1high simian immunodeficiency virus-specific CD8 T cells in the face of rapid clearance
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 87:9836-44. 2013
    ..Our data suggest that the persistence of PD-1(high) SIV-specific CD8 T cells in chronic infection is maintained in vivo by a mechanism involving high production coupled with a high disappearance rate. ..
  16. pmc T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesis
    Jason M Brenchley
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 78:1160-8. 2004
    ..These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection...
  17. pmc In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins
    Hiroshi Fujiwara
    Stem Cell Allotransplant Section, Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:4495-503. 2005
    ..This study emphasizes the clinical potential of PGP for expansion and adoptive transfer of polyclonal leukemia antigen-specific T cells to treat leukemia...
  18. pmc Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infection
    Joseph P Casazza
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    PLoS Pathog 5:e1000646. 2009
    ..These data suggest that CD4+ T cells which produce MIP-1alpha and MIP-1beta bind these chemokines in an autocrine fashion which decreases the risk of in vivo HIV infection...
  19. pmc High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrow
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 113:2238-44. 2009
    ..These data suggest that concomitant examination of bone marrow specimens in patients with myeloid leukemias might yield more definitive information in the search for immunologic prognosticators of clinical outcome...
  20. doi request reprint Evaluation of the pathogenesis of decreasing CD4(+) T cell counts in human immunodeficiency virus type 1-infected patients receiving successfully suppressive antiretroviral therapy
    Elizabeth Nies-Kraske
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, MD, USA
    J Infect Dis 199:1648-56. 2009
    ..All 4 patients had significant fibrosis of the T cell zone of lymphoid tissue, which appeared to be an important factor in the failure to reconstitute T cells...
  21. pmc Alloreactivity across HLA barriers is mediated by both naïve and antigen-experienced T cells
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Biol Blood Marrow Transplant 17:800-9. 2011
    ....
  22. ncbi request reprint Systemic vaccination prevents the total destruction of mucosal CD4 T cells during acute SIV challenge
    Joseph J Mattapallil
    Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
    J Med Primatol 35:217-24. 2006
    ..The primary objective of this study was to determine if systemic vaccination with DNA/rAd-5 encoding SIV-mac239-env, gag and pol could prevent the destruction of CD4 T cells in mucosal tissues...
  23. ncbi request reprint Interleukin-15 but not interleukin-7 abrogates vaccine-induced decrease in virus level in simian immunodeficiency virus mac251-infected macaques
    Anna Hryniewicz
    Animal Models and Retroviral Vaccines Section, National Cancer Institute, Building 41, Bethesda, MD 20892, USA
    J Immunol 178:3492-504. 2007
    ..These results underscore the importance of testing immunomodulatory approaches in vivo to assess potential risks and benefits for HIV-1-infected individuals...
  24. pmc Minor viral and host genetic polymorphisms can dramatically impact the biologic outcome of an epitope-specific CD8 T-cell response
    Christof Geldmacher
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA
    Blood 114:1553-62. 2009
    ..Thus, minor viral and host genetic polymorphisms can dramatically alter the immunologic and virologic outcome of an epitope-specific CD8 T-cell response...
  25. pmc Virological outcome after structured interruption of antiretroviral therapy for human immunodeficiency virus infection is associated with the functional profile of virus-specific CD8+ T cells
    Marybeth Daucher
    National Institute of Allergy and Infectious Diseases, Bldg 10 Rm 11B13, Bethesda, MD 20892, USA
    J Virol 82:4102-14. 2008
    ....
  26. pmc CD4 T follicular helper cell dynamics during SIV infection
    Constantinos Petrovas
    Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    J Clin Invest 122:3281-94. 2012
    ..Therefore, chronic SIV does not disturb the ability of TFH cells to help B cell maturation and production of SIV-specific immunoglobulins...
  27. pmc Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infection
    Takuya Yamamoto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    J Virol 86:5877-84. 2012
    ..The ability to elicit such virus-specific EM CD8(+) T cells might contribute substantially to an efficacious HIV/AIDS vaccine, even after breakthrough infection...
  28. pmc Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infection
    Michael R Betts
    Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:4512-7. 2005
    ..These data suggest that control of HIV by vaccine-elicited HIV-specific T cell responses may be difficult, even when the T cell response has those characteristics predicted to provide optimal protection...
  29. pmc Differential Th17 CD4 T-cell depletion in pathogenic and nonpathogenic lentiviral infections
    Jason M Brenchley
    Human Immunology Section, Vaccine Research Center VRC, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD, USA
    Blood 112:2826-35. 2008
    ..Finally, these data may help account for the nonprogressive nature of nonpathogenic SIV infection in sooty mangabeys...
  30. pmc In vitro culture during retroviral transduction improves thymic repopulation and output after total body irradiation and autologous peripheral blood progenitor cell transplantation in rhesus macaques
    Karin Lore
    Immunology Laboratory, Research Center, National Institute of Allergy and Infectious Diseases, NIH, Department of Health and Human Services, Bethesda, Maryland 20892 1202, USA
    Stem Cells 24:1539-48. 2006
    ..These results have implications for the design of gene therapy trials, as well as for the use of expanded PBPCs for improved T-cell immune reconstitution after transplantation...
  31. ncbi request reprint T cell receptor recognition motifs govern immune escape patterns in acute SIV infection
    David A Price
    Human Immunology Section, Vaccine Research Center, NIAID NIH, Bethesda, MD 20892, USA
    Immunity 21:793-803. 2004
    ..These findings have profound implications for the development of vaccines that elicit T cell immunity to combat pathogens with unstable genomes...
  32. ncbi request reprint The clonal composition of human CD4+CD25+Foxp3+ cells determined by a comprehensive DNA-based multiplex PCR for TCRB gene rearrangements
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol Methods 321:107-20. 2007
    ..Clonotypes shared between the CD4+CD25+Foxp3+ and CD4+CD25+Foxp3- subsets were observed in all 3 UCB and in one adult PBMCs, suggesting that naïve and memory CD4+ T(R) can share the same clonotypes as CD4+ non-T(R) in humans...
  33. pmc Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses
    Melissa L Precopio
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1405-16. 2007
    ..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated...
  34. pmc Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge
    Joseph J Mattapallil
    Vaccine Research Center, National Institute of Allergy and Infectious Disease NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:1533-41. 2006
    ....
  35. ncbi request reprint Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection
    Joseph J Mattapallil
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 434:1093-7. 2005
    ..Our findings point to the importance of reducing the cell-associated viral load during acute infection through therapeutic or vaccination strategies...
  36. ncbi request reprint Imatinib inhibits T-cell receptor-mediated T-cell proliferation and activation in a dose-dependent manner
    Ruth Seggewiss
    Hematology Branch, National Heart, Lung, and Blood Institute NHLBI, the Immunology Laboratory, NIH DHHS, Bldg 10, CRC, Rm 4 5140, 10 Center Dr, MSC 1202, Bethesda, MD 20892, USA
    Blood 105:2473-9. 2005
    ....
  37. pmc Preferential infection shortens the life span of human immunodeficiency virus-specific CD4+ T cells in vivo
    Jason M Brenchley
    Human Virology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    J Virol 80:6801-9. 2006
    ....
  38. pmc Immunologic pressure within class I-restricted cognate human immunodeficiency virus epitopes during highly active antiretroviral therapy
    Joseph P Casazza
    Immunology Laboratory, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 79:3653-63. 2005
    ....
  39. ncbi request reprint Flow cytometric analysis of human antigen-specific T-cell proliferation
    Jason M Brenchley
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20874, USA
    Methods Cell Biol 75:481-96. 2004
  40. ncbi request reprint T-cell responses directed against multiple HLA-A*0201-restricted epitopes derived from Wilms' tumor 1 protein in patients with leukemia and healthy donors: identification, quantification, and characterization
    Katayoun Rezvani
    Stem Cell Allotransplantation Section, Hematology Branch, National Heart Lung Blood Institute and Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:8799-807. 2005
    ..Four HLA-A*0201-restricted WT1-derived epitopes have been identified: WT37, WT126, WT187, and WT235. We determined the natural immunogenecity of these antigens in patients with hematologic malignancies and healthy donor...
  41. pmc Detection of low avidity CD8(+) T cell populations with coreceptor-enhanced peptide-major histocompatibility complex class I tetramers
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol Methods 338:31-9. 2008
    ....
  42. ncbi request reprint Identification and in vitro expansion of CD4+ and CD8+ T cells specific for human neutrophil elastase
    Hiroshi Fujiwara
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 103:3076-83. 2004
    ..HNE could, therefore, be used in an HLA-unrestricted manner to induce leukemia-reactive CTLs for adoptive immunotherapy...
  43. pmc Keratinocyte growth factor augments immune reconstitution after autologous hematopoietic progenitor cell transplantation in rhesus macaques
    Ruth Seggewiss
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Blood 110:441-9. 2007
    ..Thus, our findings suggest that KGF may be a useful adjuvant therapy to augment T-cell reconstitution after human PBPC transplantation...
  44. pmc PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 203:2281-92. 2006
    ....
  45. ncbi request reprint Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells
    Jason M Brenchley
    Vaccine Research Center and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:2711-20. 2003
    ..Thus, our studies define a phenotype associated with replicative senescence in HIV-specific CD8(+) T cells, which may have broad implications to other conditions associated with chronic antigenic stimulation...
  46. ncbi request reprint Alloreactive T cell clonotype recruitment in a mixed lymphocyte reaction: implications for graft engineering
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1202, USA
    Exp Hematol 34:788-95. 2006
    ..To better understand the alloresponse between HLA-identical related pairs, we characterized the alloreactive T cells generated in a mixed lymphocyte reaction (MLR) assay system...
  47. pmc Evolution of the donor T-cell repertoire in recipients in the second decade after allogeneic stem cell transplantation
    Robert Quan Le
    Stem Cell Allotransplantation Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 117:5250-6. 2011
    ..This study is registered at http://www.clinicaltrials.gov as NCT00106925...
  48. ncbi request reprint HIV-infected Langerhans cells preferentially transmit virus to proliferating autologous CD4+ memory T cells located within Langerhans cell-T cell clusters
    Makoto Sugaya
    Dermatology Branch, Center for Cancer Research, National Cancer Institute, and Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    J Immunol 172:2219-24. 2004
    ..Disrupting cluster formation between LC and memory CD4(+) T cells may be a novel strategy to interfere with sexual transmission of HIV...
  49. ncbi request reprint Microbial translocation is a cause of systemic immune activation in chronic HIV infection
    Jason M Brenchley
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Med 12:1365-71. 2006
    ..These data establish a mechanism for chronic immune activation in the context of a compromised gastrointestinal mucosal surface and provide new directions for therapeutic interventions that modify the consequences of acute HIV infection...
  50. pmc Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infection
    Takuya Yamamoto
    National Institutes of Health, Bethesda, MD, USA
    Blood 117:4805-15. 2011
    ..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons...
  51. ncbi request reprint CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response
    Hongwei H Zhang
    Inflammation Biology Section, Laboratory of Molecular Immunology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 185:6646-63. 2010
    ....
  52. pmc Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans
    Kaimei Song
    Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7916-21. 2005
    ..Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells...
  53. ncbi request reprint Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
    ....
  54. ncbi request reprint IL-7 therapy dramatically alters peripheral T-cell homeostasis in normal and SIV-infected nonhuman primates
    Terry J Fry
    Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 101:2294-9. 2003
    ..These results also raise the possibility that IL-7 therapy could be used to temporarily modulate T-cell cycling in vivo in the context of immunotherapies such as vaccination...
  55. pmc Reduction of immune activation with chloroquine therapy during chronic HIV infection
    Shannon M Murray
    Human Immunology Section, NIH Vaccine Research Center, Bethesda, MD 20892 3005, USA
    J Virol 84:12082-6. 2010
    ..Our data indicate that treatment with CQ reduces systemic T-cell immune activation and, thus, that its use may be beneficial for certain groups of HIV-infected individuals...
  56. pmc Cytopenia and leukocyte recovery shape cytokine fluctuations after myeloablative allogeneic hematopoietic stem cell transplantation
    Jan Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Haematologica 97:867-73. 2012
    ..The aim of this study was to examine the contributions of the conditioning regimen, donor engraftment, infections, and graft-versus-host disease to fluctuations in cytokines involved in homeostasis and inflammation...
  57. pmc Convergent recombination shapes the clonotypic landscape of the naive T-cell repertoire
    Maire F Quigley
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:19414-9. 2010
    ..These findings provide a framework for understanding the early mobilization of public CD8(+) T-cell clonotypes, which can exert profound biological effects during acute infectious processes...
  58. pmc Host response to translocated microbial products predicts outcomes of patients with HBV or HCV infection
    Netanya G Sandler
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Gastroenterology 141:1220-30, 1230.e1-3. 2011
    ..We investigated whether the extent and progression of liver disease in patients with chronic HBV or HCV infection are associated with microbial translocation and subsequent activation of monocytes...
  59. ncbi request reprint IFN-gamma mediates the death of Th1 cells in a paracrine manner
    Kathryn E Foulds
    Cellular Immunology Section, Human Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 180:842-9. 2008
    ..Collectively, these data show the multiple mechanisms by which Th1 effector cells are efficiently eliminated in vivo...
  60. pmc Emerging concepts in the immunopathogenesis of AIDS
    Daniel C Douek
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Med 60:471-84. 2009
    ....
  61. pmc Longitudinal assessment of de novo T cell production in relation to HIV-associated T cell homeostasis failure
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    AIDS Res Hum Retroviruses 22:501-7. 2006
    ..These results suggest that deficits in de novo T cell production, either through the decline of thymic function or the destruction of naive T cells, are likely to play an important role in TCH failure and progression of HIV-1 disease...
  62. ncbi request reprint Early reconstitution of the T-cell repertoire after non-myeloablative peripheral blood stem cell transplantation is from post-thymic T-cell expansion and is unaffected by graft-versus-host disease or mixed chimaerism
    Erkut Bahceci
    Bone Marrow Transplant Unit, Hematology Branch, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Br J Haematol 122:934-43. 2003
    ..These results define important qualitative differences in the T-cell repertoire according to the type of transplant schedule used...
  63. pmc Vaccine design for CD8 T lymphocyte responses
    Richard A Koup
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cold Spring Harb Perspect Med 1:a007252. 2011
    ....
  64. pmc HLA B*5701-positive long-term nonprogressors/elite controllers are not distinguished from progressors by the clonal composition of HIV-specific CD8+ T cells
    Daniel Mendoza
    HIV Specific Immunity Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:4014-8. 2012
    ..Thus, HIV-specific CD8(+) T-cell responses in LTNP/EC are not differentiated from those of progressors on the basis of clonal diversity or TCR sharing...
  65. pmc Cellular immunity elicited by human immunodeficiency virus type 1/ simian immunodeficiency virus DNA vaccination does not augment the sterile protection afforded by passive infusion of neutralizing antibodies
    John R Mascola
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:10348-56. 2003
    ..These data suggest that although effector T cells can limit viral replication, they are not able to assist humoral immunity to prevent the establishment of initial infection...
  66. ncbi request reprint Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation
    Katayoun Rezvani
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 102:2892-900. 2003
    ..The increased response in patients after SCT suggests a quantitative explanation for the greater effect of allogeneic SCT...
  67. ncbi request reprint Lymphopenia and interleukin-2 therapy alter homeostasis of CD4+CD25+ regulatory T cells
    Hua Zhang
    Pediatric Oncology Branch, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892, USA
    Nat Med 11:1238-43. 2005
    ..These studies suggest that IL-2 and lymphopenia are primary modulators of CD4(+)CD25(+) T(reg) cell homeostasis...
  68. ncbi request reprint HIV disease: fallout from a mucosal catastrophe?
    Jason M Brenchley
    Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Immunol 7:235-9. 2006
    ....
  69. pmc Downregulation of robust acute type I interferon responses distinguishes nonpathogenic simian immunodeficiency virus (SIV) infection of natural hosts from pathogenic SIV infection of rhesus macaques
    Levelle D Harris
    Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, Maryland, USA
    J Virol 84:7886-91. 2010
    ..In contrast, persistently high type I IFN responses are observed during pathogenic SIV infection of rhesus macaques...
  70. pmc T-cell immune responses to Wilms tumor 1 protein in myelodysplasia responsive to immunosuppressive therapy
    Elaine M Sloand
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 117:2691-9. 2011
    ..Thus, our results suggest that WT1 is one of the antigens that triggers T cell-mediated myelosuppression in MDS...
  71. pmc CD4+ T cell depletion during all stages of HIV disease occurs predominantly in the gastrointestinal tract
    Jason M Brenchley
    Human Immunology Section, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Dr, Room 3509, Bethesda, MD 20892, USA
    J Exp Med 200:749-59. 2004
    ....
  72. pmc Evidence for translocation of microbial products in patients with idiopathic CD4 lymphocytopenia
    Philip I Lee
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Infect Dis 199:1664-70. 2009
    ..These findings suggest a potential association of translocation of microbial products with perturbed CD4 T cell homeostasis in individuals with CD4 lymphopenic states other than HIV infection...
  73. ncbi request reprint Renewing the T cell repertoire to arrest autoimmune aggression
    Paolo A Muraro
    Neuroimmunology Branch, NINDS NIH, Bldg 10, Room 5B16, 10 Center Drive, MSC1400, Bethesda, MD 20892 1400, USA
    Trends Immunol 27:61-7. 2006
    ..We propose a model envisioning a coordinated sequence of events, rebuilding an immune system that is competent against infection but that is substantially reconfigured in a way that is less likely to redevelop autoimmunity...
  74. pmc Hypomorphic Rag mutations can cause destructive midline granulomatous disease
    Suk See De Ravin
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 116:1263-71. 2010
    ..This study was registered at www.clinicaltrials.gov as #NCT00128973...
  75. pmc Nonprogressive and progressive primate immunodeficiency lentivirus infections
    Jason M Brenchley
    Immunopathogenesis Unit, Lab of Molecular Microbiology, NIAID, NIH, Bethesda, MD 20892, USA
    Immunity 32:737-42. 2010
    ....
  76. ncbi request reprint The rational design of an AIDS vaccine
    Daniel C Douek
    Vaccine Research Center, NIAID, National Institutes of Health, Room 4502, Building 40, MSC 3005, 40 Convent Drive, Bethesda, MD 20892, USA
    Cell 124:677-81. 2006
    ..AIDS vaccine design requires a scientifically driven, rational approach that encompasses the latest advances in viral molecular genetics, structural biology, and immunology...
  77. ncbi request reprint Effects of exogenous interleukin-7 on human thymus function
    Yukari Okamoto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases and Department of Experimental Transplantation and Immunology, Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 99:2851-8. 2002
    ..Our results provide compelling evidence that IL-7 has a direct effect on increasing TCR-alphabeta rearrangement and indicate the potential use of IL-7 for enhancing de novo naïve T-cell generation in immunocompromised patients...
  78. pmc Damaged intestinal epithelial integrity linked to microbial translocation in pathogenic simian immunodeficiency virus infections
    Jacob D Estes
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland, USA
    PLoS Pathog 6:e1001052. 2010
    ..Novel therapeutic approaches to inhibit microbial translocation and/or attenuate chronic immune activation in HIV-infected individuals may complement treatments aimed at direct suppression of viral replication...
  79. pmc Human immunodeficiency virus type 1 neutralization measured by flow cytometric quantitation of single-round infection of primary human T cells
    John R Mascola
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 76:4810-21. 2002
    ..The precision and reproducibility of this assay will facilitate the measurement of HIV-1 neutralization, particularly incrementally improved neutralizing antibody responses generated by new candidate vaccines...
  80. pmc Thymic output generates a new and diverse TCR repertoire after autologous stem cell transplantation in multiple sclerosis patients
    Paolo A Muraro
    Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 201:805-16. 2005
    ....
  81. ncbi request reprint Induction and evolution of cytomegalovirus-specific CD4+ T cell clonotypes in rhesus macaques
    David A Price
    Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 180:269-80. 2008
    ....
  82. ncbi request reprint Contribution of TCR-beta locus and HLA to the shape of the mature human Vbeta repertoire
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 180:6484-9. 2008
    ..We therefore conclude that the correlation in Vbeta expression patterns between CD4(+) and CD8(+) T cells can be explained predominantly by germline TCR-beta locus factors and not TCR-beta allelic or HLA effects...
  83. pmc Impaired T(H)17 cell differentiation in subjects with autosomal dominant hyper-IgE syndrome
    Joshua D Milner
    Laboratory of Immunology, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 452:773-6. 2008
    ..Thus, our data suggest that the inability to produce T(H)17 cells is a mechanism underlying the susceptibility to the recurrent infections commonly seen in HIES...
  84. ncbi request reprint Factors influencing T-lymphopoiesis after allogeneic hematopoietic cell transplantation
    Jan Storek
    Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Transplantation 73:1154-8. 2002
    ..without irradiation), acute graft-versus-host disease (aGVHD), or chronic graft-versus-host disease (cGVHD) in multivariate analysis. We conclude that patient age is the primary determinant of CD4 T-lymphopoiesis after allogeneic HCT...
  85. ncbi request reprint Adult human liver contains CD8pos T cells with naive phenotype, but is not a site for conventional alpha beta T cell development
    Lucy Golden-Mason
    Education and Research Center, St Vincent s University Hospital, Dublin, Ireland
    J Immunol 172:5980-5. 2004
    ..The almost complete absence of TRECs suggests that normal AHL is not a site for the development of conventional alphabeta T cells...
  86. ncbi request reprint Direct ex vivo analysis of human CD4(+) memory T cell activation requirements at the single clonotype level
    Arlene D Bitmansour
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland, OR 97201, USA
    J Immunol 169:1207-18. 2002
    ....
  87. ncbi request reprint Where does HIV live?
    Justin Stebbing
    Department of Immunology, Division of Investigative Science, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Chelsea and Westminster Hospital, London
    N Engl J Med 350:1872-80. 2004
  88. ncbi request reprint T cells containing T cell receptor excision circles are inversely related to HIV replication and are selectively and rapidly released into circulation with antiretroviral treatment
    Mireya Diaz
    Department of Epidemiology and Biostatistics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    AIDS 17:1145-9. 2003
    ..To examine baseline predictors of T-cell receptor rearrangement excision circle (TREC) levels and their changes during treatment with combined antiretroviral therapy...
  89. pmc Increased thymic output during acute measles virus infection
    Sallie R Permar
    W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205 2179, USA
    J Virol 77:7872-9. 2003
    ..These findings suggest that a decrease in thymic output is not the cause of the lymphopenia and depressed cellular immunity associated with measles...
  90. ncbi request reprint High prevalence of autoreactive, neuroantigen-specific CD8+ T cells in multiple sclerosis revealed by novel flow cytometric assay
    Michael P Crawford
    Department of Pathology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9072, USA
    Blood 103:4222-31. 2004
    ..The results emphasize the need to evaluate both CD4(+) and CD8(+) T-cell responses in MS and to make both subsets a consideration in the development of novel therapeutic strategies...
  91. ncbi request reprint Unusual immunophenotype of CD8+ T cells in familial hemophagocytic lymphohistiocytosis
    Nitin J Karandikar
    Department of Pathology, The University of Texas Southwestern Medical Center at Dallas, 75390 9072, USA
    Blood 104:2007-9. 2004
    ..This case provides an instructive example of uncontrolled reactive proliferation of CD8+ T cells in FHL, resulting in atypical morphology and unusual immunophenotypic features that might suggest malignancy in other clinical settings...
  92. pmc Glatiramer acetate (Copaxone) therapy induces CD8(+) T cell responses in patients with multiple sclerosis
    Nitin J Karandikar
    Department of Pathology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9072, USA
    J Clin Invest 109:641-9. 2002
    ....
  93. pmc Acute loss of intestinal CD4+ T cells is not predictive of simian immunodeficiency virus virulence
    Ivona V Pandrea
    Divisions of Comparative Pathology and Microbiology, Tulane National Primate Research Center, 18703 Three Rivers Road, Covington, LA 70433, USA
    J Immunol 179:3035-46. 2007
    ....
  94. pmc Validation of RNA-based molecular clonotype analysis for virus-specific CD8+ T-cells in formaldehyde-fixed specimens isolated from peripheral blood
    David van Bockel
    Centre for Immunology, St Vincent s Hospital, Sydney, NSW, Australia
    J Immunol Methods 326:127-38. 2007
    ....
  95. pmc Progressive CD4+ central memory T cell decline results in CD4+ effector memory insufficiency and overt disease in chronic SIV infection
    Afam Okoye
    Vaccine and Gene Therapy Institute, Department of Pathology, and the Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA
    J Exp Med 204:2171-85. 2007
    ....
  96. pmc Comparison of plasma viremia and antibody responses in macaques inoculated with envelope variants of single-cycle simian immunodeficiency virus differing in infectivity and cellular tropism
    M Quinn DeGottardi
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, One Pine Hill Drive, Southborough, MA 01772 9102, USA
    J Virol 82:321-34. 2008
    ..These studies reveal differences in the peaks and durations of a single round of productive infection that reflect envelope-specific differences in infectivity, chemokine receptor specificity, and cellular tropism...
  97. ncbi request reprint Differential selection pressure exerted on HIV by CTL targeting identical epitopes but restricted by distinct HLA alleles from the same HLA supertype
    Alasdair Leslie
    Department of Paediatrics, Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, South Parks Road, Oxford OX1 3SY, United Kingdom
    J Immunol 177:4699-708. 2006
    ..These findings are of relevance to vaccine approaches that seek to exploit HLA supertypes to overcome the problem of HLA diversity...
  98. pmc CD127 and CD25 expression defines CD4+ T cell subsets that are differentially depleted during HIV infection
    Richard M Dunham
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 180:5582-92. 2008
    ..Further studies will determine whether monitoring the three subsets of CD4(+) T cells defined based on the expression of CD25 and CD127 should be used in the clinical management of HIV-infected individuals...
  99. doi request reprint CD8+ T cell efficacy in vaccination and disease
    Victor Appay
    Cellular Immunology Laboratory, Institut Nationale de la Santé et de la Recherche Médicale U543, Avenir Group, Hopital Pitie Salpetriere, Universite Pierre et Marie Curie Paris 06, 91 Boulevard de l Hopital, 75013 Paris, France
    Nat Med 14:623-8. 2008
    ..This new knowledge paves the way to the design of more effective T cell vaccines and highlights the importance of comprehensive immunomonitoring...
  100. ncbi request reprint Maintenance of HIV-specific CD4+ T cell help distinguishes HIV-2 from HIV-1 infection
    Melody G Duvall
    Medical Research Council MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    J Immunol 176:6973-81. 2006
    ..This study demonstrates that HIV-2-infected donors have a well-preserved and functionally heterogeneous HIV-specific memory CD4+ T cell response that is associated with delayed disease progression in the majority of infected people...
  101. ncbi request reprint The role of production frequency in the sharing of simian immunodeficiency virus-specific CD8+ TCRs between macaques
    Vanessa Venturi
    Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Kensington, Australia
    J Immunol 181:2597-609. 2008
    ..Thus, convergent recombination is a major determinant of the extent of TCRbeta sharing...