Research Topics
| J G DonaldsonSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosisMarco Scarselli
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:3577-85. 2009..These findings demonstrate that GPCRs are versatile PM proteins that can utilize different mechanisms of internalization depending upon ligand activation...- Arf6 recruits the Rac GEF Kalirin to the plasma membrane facilitating Rac activationTae Hyeon Koo
Laboratory of Cell Biology, NHLBI, NIH, Bethesda, MD 20892, USA
BMC Cell Biol 8:29. 2007..Although Arf6 facilitates the trafficking of Rac1 to the plasma membrane and in many cases Arf6 activation leads to the activation of Rac1, the details of how Arf6 influences Rac function remain to be elucidated... - ARF family G proteins and their regulators: roles in membrane transport, development and diseaseJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Rev Mol Cell Biol 12:362-75. 2011..Important advances are being gained in our understanding of the functional networks that are formed not only by the GEFs and GAPs themselves but also by the inactive forms of the ARF proteins... 
Arfs, phosphoinositides and membrane trafficJ G Donaldson
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Rm 2503, Bethesda, MD 20892, USA
Biochem Soc Trans 33:1276-8. 2005..Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex...- Multiple activities for Arf1 at the Golgi complexJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 2503, Bethesda, MD 20892, USA
Biochim Biophys Acta 1744:364-73. 2005..This spatial distribution of Arf regulators may facilitate the recruitment of the coat proteins and other Arf effectors to different regions of the Golgi complex... - Multiple roles for Arf6: sorting, structuring, and signaling at the plasma membraneJulie G Donaldson
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Building 50 Room 2503, Bethesda, MD 20892, USA
J Biol Chem 278:41573-6. 2003 - Regulators and effectors of the ARF GTPasesJ G Donaldson
Laboratory of Cell Biology, NHLBI, NIH, Bethesda, 20892, USA
Curr Opin Cell Biol 12:475-82. 2000..In addition, two new classes of effectors, the PIP kinases and a novel family of monomeric coat-like proteins have been discovered... - Clathrin-independent endocytosis: a unique platform for cell signaling and PM remodelingJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Cell Signal 21:1-6. 2009..Furthermore, activation of some of these signaling molecules (H-Ras, Rac and Arf6) can switch the constitutive form of clathrin-independent endocytosis into a stimulated one, associated with PM ruffling and macropinocytosis... 
Arfs and membrane lipids: sensing, generating and responding to membrane curvatureJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bld 50, Rm 2503, Bethesda, MD 20892, USA
Biochem J 414:e1-2. 2008..These findings suggest that Arf protein activation and membrane interaction may initiate membrane curvature that will be enhanced further by coat proteins during vesicle formation...- Phospholipase D in endocytosis and endosomal recycling pathwaysJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 2503, Bethesda, MD 20892, USA
Biochim Biophys Acta 1791:845-9. 2009..This review examines studies that have reported a role for PLD in endocytosis and membrane recycling from endocytic pathways... - Localization and function of Arf family GTPasesJ G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Soc Trans 33:639-42. 2005..Whereas Arf6 is targeted to the plasma membrane through multiple regions along the protein, we have found a Golgi-targeting region in Arf1 that is sufficient to target Arf6 to the Golgi complex... - ACAPs are arf6 GTPase-activating proteins that function in the cell peripheryT R Jackson
Laboratory of Cellular Oncology, Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
J Cell Biol 151:627-38. 2000..The additional effects of ASAP1 on PDGF-induced ruffling in fibroblasts suggest that multiple Arf GAPs function coordinately in the cell periphery... - Separation of membrane trafficking and actin remodeling functions of ARF6 with an effector domain mutantO Al-Awar
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 20:5998-6007. 2000..Thus, we have uncoupled two functions of ARF6, one involved in membrane trafficking, which is necessary for Rac ruffling, and another involved in protrusion formation... - Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatusJ G Donaldson
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
J Cell Biol 112:579-88. 1991.... - ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangementsH Radhakrishna
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 112:855-66. 1999..These observations suggest that ARF6, a non-Rho family GTPase, can, by itself, alter cortical actin and can influence the ability of Rac1 to form lamellipodia, in part, by regulating its trafficking to the plasma membrane... - Building a secretory apparatus: role of ARF1/COPI in Golgi biogenesis and maintenanceJ Lippincott-Schwartz
Cell Biology and Metabolism Branch, NICHD, NIH, Bethesda, MD 20892 5430, USA
Histochem Cell Biol 109:449-62. 1998.... - Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domainsLee Ann Cohen
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, and Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 18:2244-53. 2007..This interaction was direct and required both inositol phospholipids and GTP. We propose a model of sequential Arf activation at the PM whereby Arf6-GTP recruits ARNO family GEFs for further activation of other Arf isoforms... - Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane trafficF D Brown
Laboratory of Cell Biology, National Heart Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA
J Cell Biol 154:1007-17. 2001..These results demonstrate that PIP 5-kinase activity and PIP2 turnover controlled by activation and inactivation of Arf6 is critical for trafficking through the Arf6 PM-endosomal recycling pathway... - Convergence of non-clathrin- and clathrin-derived endosomes involves Arf6 inactivation and changes in phosphoinositidesNaava Naslavsky
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Biol Cell 14:417-31. 2003..A similar block in transport of MHCI and Tac was reversibly induced by a PI3-kinase inhibitor, implying that inactivation of Arf6 and acquisition of PI3P are required for convergence of endosomes arising from these two pathways... - A unique platform for H-Ras signaling involving clathrin-independent endocytosisNatalie Porat-Shliom
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 19:765-75. 2008..Arf6 stimulation of macropinocytosis also involves passage through the distinct lipid phases, but recruitment of Akt is not observed... - Recycling of Raft-associated prohormone sorting receptor carboxypeptidase E requires interaction with ARF6Irina Arnaoutova
Section of Cellular Neurobiology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Biol Cell 14:4448-57. 2003..Thus, CPE recycles back to the TGN by a novel mechanism requiring ARF6 interaction and activity... - Discovery of new cargo proteins that enter cells through clathrin-independent endocytosisCraig A Eyster
Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
Traffic 10:590-9. 2009..This divergent itinerary suggests that sorting may occur along this CIE pathway. Furthermore, the identification of new cargo proteins will assist others studying CIE in different cell types and tissues... - An effector domain mutant of Arf6 implicates phospholipase D in endosomal membrane recyclingOlivera A Jovanovic
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biol Cell 17:327-35. 2006..Together, these observations provide compelling evidence that Arf6 stimulation of PLD is required for endosomal membrane recycling and GAP recruitment... - Characterization of a nonclathrin endocytic pathway: membrane cargo and lipid requirementsNaava Naslavsky
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Biol Cell 15:3542-52. 2004..Endocytosis of transferrin was not affected by any of these treatments. These observations suggest common mechanisms for endocytosis without clathrin... - Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNAREAkira Honda
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Biol 168:1039-51. 2005..These studies suggest that membrin recruits Arf-1 to the early Golgi and reveal distinct kinetic cycles for Arf-1 at early and late Golgi determined by different sets of Arf regulators and effectors... - A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membraneSteve Caplan
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 21:2557-67. 2002..These observations suggest an additional function of EHD1 as a tubule-inducing factor in the Arf6 pathway for recycling of plasma membrane proteins internalized by clathrin-independent endocytosis... - Rab22a regulates the recycling of membrane proteins internalized independently of clathrinRoberto Weigert
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 8017, USA
Mol Biol Cell 15:3758-70. 2004..Dominant negative mutant of Rab11a also inhibited recycling of MHCI but not the formation of recycling tubules, suggesting that Rab22a and Rab11a might coordinate different steps of MHCI recycling... - Brefeldin A-inhibited guanine nucleotide-exchange activity of Sec7 domain from yeast Sec7 with yeast and mammalian ADP ribosylation factorsM Sata
Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 95:4204-8. 1998..These results are consistent with the conclusion that the yeast Sec7 domain itself contains the elements necessary for ARF GEP activity and its inhibition by BFA... - Arf6 and its role in cytoskeletal modulationJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 189:191-8. 2002 - Intracellular assembly and trafficking of MHC class I moleculesJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA
Traffic 10:1745-52. 2009..We review recent progress in our understanding of class I assembly, anterograde transport and endocytosis and highlight some of the events targeted by viruses as a means to evade detection by cytotoxic T cells and natural killer cells... - Myoblasts fuse when loner meets ARF6Julie G Donaldson
Laboratory of Cell Biology, NHLBI, NIH, Building 50, Room 2503, Bethesda, MD 20892, USA
Dev Cell 5:527-8. 2003..A paper by Chen et al. published in the September 19 issue of Cell adds ARF6, a GTPase, and one of its guanine nucleotide exchange factors to this set of players... - Somatostatin receptors signal through EFA6A-ARF6 to activate phospholipase D in clonal beta-cellsJustin A Grodnitzky
Department of Biomedical Sciences, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 282:13410-8. 2007..In addition, overexpression of dn-ARNO mutant, ARNO(E156K), another GEF of Arf6, had no effect on SS-induced activation of PLD. Taken together, these results suggest that SS signals through EFA6A to activate Arf6-PLD cascade... - Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosisRoberto Weigert
Methods Enzymol 403:243-53. 2005..Here we describe cell-based flourescence assays to examine the effects of expression and depletion of Rab22 on endosomal morphology and endocytic recycling...