R E Donahue

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Leukocyte integrin activation mediates transient neutropenia after G-CSF administration
    Robert E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Rockville, MD, USA
    Blood 118:4209-14. 2011
  2. ncbi request reprint Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Hum Gene Ther 12:607-17. 2001
  3. pmc Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony-stimulating factor
    C E Dunbar
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:11871-6. 1996
  4. pmc Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands
    C Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Oral Dis 16:269-77. 2010
  5. pmc Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates
    M Takatoku
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 108:447-55. 2001
  6. ncbi request reprint Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating ability
    J F Tisdale
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Blood 92:1131-41. 1998
  7. ncbi request reprint Transplantation of transduced nonhuman primate CD34+ cells using a gibbon ape leukemia virus vector: restricted expression of the gibbon ape leukemia virus receptor to a subset of CD34+ cells
    B A Bunnell
    Clinical Gene Therapy Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
    Gene Ther 6:48-56. 1999
  8. ncbi request reprint The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells
    S E Sellers
    Hematology Branch, The National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    Blood 97:1888-91. 2001
  9. ncbi request reprint The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate model
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 938:236-44; discussion 244-5. 2001
  10. ncbi request reprint Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34+ cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 21892, USA
    Mol Ther 3:359-67. 2001

Collaborators

Detail Information

Publications19

  1. pmc Leukocyte integrin activation mediates transient neutropenia after G-CSF administration
    Robert E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Rockville, MD, USA
    Blood 118:4209-14. 2011
    ..These results demonstrate that the leukocyte integrin on circulating neutrophils is transiently activated after G-CSF administration and mediates the transient neutropenia observed after G-CSF administration...
  2. ncbi request reprint Update on the use of nonhuman primate models for preclinical testing of gene therapy approaches targeting hematopoietic cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
    Hum Gene Ther 12:607-17. 2001
    ..Judicious application of these models should continue to be a priority, and advances should now be tested in proof-of-concept clinical trials...
  3. pmc Improved retroviral gene transfer into murine and Rhesus peripheral blood or bone marrow repopulating cells primed in vivo with stem cell factor and granulocyte colony-stimulating factor
    C E Dunbar
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 93:11871-6. 1996
    ....
  4. pmc Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands
    C Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Oral Dis 16:269-77. 2010
    ..This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands...
  5. pmc Avoidance of stimulation improves engraftment of cultured and retrovirally transduced hematopoietic cells in primates
    M Takatoku
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 108:447-55. 2001
    ..We observed stable in vivo overall gene marking levels of up to 29%. This approach may allow more efficient engraftment of transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion...
  6. ncbi request reprint Ex vivo expansion of genetically marked rhesus peripheral blood progenitor cells results in diminished long-term repopulating ability
    J F Tisdale
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Blood 92:1131-41. 1998
    ..However, a brief transduction in the presence of specific cytokines and stromal support allows engraftment with an encouraging number of retrovirally modified cells...
  7. ncbi request reprint Transplantation of transduced nonhuman primate CD34+ cells using a gibbon ape leukemia virus vector: restricted expression of the gibbon ape leukemia virus receptor to a subset of CD34+ cells
    B A Bunnell
    Clinical Gene Therapy Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
    Gene Ther 6:48-56. 1999
    ..Our findings suggest that, in rhesus macaques, PG13-derived retroviral vectors may only be able to transduce a subset of CD34+ cells as only CD34+ Thy-1+ cells express the GaLV receptor...
  8. ncbi request reprint The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells
    S E Sellers
    Hematology Branch, The National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, The National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD 20892, USA
    Blood 97:1888-91. 2001
    ..There was no evidence for expansion of MDR1 vector-transduced cells. Long-term engraftment with MDR1 vector- and neo vector-transduced cells occurred despite prolonged culture...
  9. ncbi request reprint The impact of ex vivo cytokine stimulation on engraftment of primitive hematopoietic cells in a non-human primate model
    C E Dunbar
    Molecular Hematopoiesis Section, Hematology Branch, NHLBI, NIH, Building 10, Room 7C103, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Ann N Y Acad Sci 938:236-44; discussion 244-5. 2001
    ..This approach may allow more efficient engraftment of successfully transduced or ex vivo expanded cells by avoiding active cell cycling at the time of reinfusion...
  10. ncbi request reprint Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34+ cells
    R E Donahue
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 21892, USA
    Mol Ther 3:359-67. 2001
    ..In the presence of CH-296, apoptosis of CD34+ cells was inhibited, and in mobilized peripheral blood CD34+ cells, cell division was stimulated as measured by cell history/tracking experiments...
  11. ncbi request reprint Efficient in vivo marking of primary CD4+ T lymphocytes in nonhuman primates using a gibbon ape leukemia virus-derived retroviral vector
    B A Bunnell
    Clinical Gene Therapy Branch, National Center for Human Genome Research, Bethesda, MD 20850, USA
    Blood 89:1987-95. 1997
    ..No antibody response was detected to the neomycin-resistance (Neo(R)) transgene, the murine retroviral group-specific antigen (gag), or GaLV envelope (env) proteins...
  12. pmc Recombinant, replication-defective adenovirus gene transfer vectors induce cell cycle dysregulation and inappropriate expression of cyclin proteins
    R P Wersto
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 72:9491-502. 1998
    ..Consequently, E4 viral gene products present in DeltaE1 or DeltaE1 DeltaE3 Ad vectors induce G2 growth arrest, which may pose new and unintended consequences for human gene transfer and gene therapy...
  13. ncbi request reprint Assessment of rapid remobilization intervals with G-CSF and SCF in murine and rhesus macaque models
    P A Shi
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892 1652, USA
    Transfusion 41:1438-44. 2001
    ..Defining the optimum regimen and time for repeat peripheral blood progenitor cell mobilization would have important clinical applications...
  14. ncbi request reprint Analysis of origin and optimization of expansion and transduction of circulating peripheral blood endothelial progenitor cells in the rhesus macaque model
    J Hu
    Hematology Branch, National Heart, Lung, and Blood Institute NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Hum Gene Ther 13:2041-50. 2002
    ..Further elucidation of the origin and in vivo behavior of EPCs may be possible, using optimized transduction conditions and a vascular injury model...
  15. pmc AAV5-mediated gene transfer to the parotid glands of non-human primates
    A Voutetakis
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892 1190, USA
    Gene Ther 17:50-60. 2010
    ..The aggregate data indicate that results with AAV5 vectors in murine salivary glands apparently do not extend to macaque glands...
  16. ncbi request reprint Hydroxyurea-induced HbF production in anemic primates: augmentation by erythropoietin, hematopoietic growth factors, and sodium butyrate
    K T McDonagh
    Clinical Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892
    Exp Hematol 20:1156-64. 1992
    ..Thus, our studies have identified several agents that may prove useful in combination with hydroxyurea to achieve clinically beneficial levels of HbF...
  17. doi request reprint Evaluation of engraftment and immunological tolerance after reduced intensity conditioning in a rhesus hematopoietic stem cell gene therapy model
    N Uchida
    Molecular and Clinical Hematology Branch, National Heart Lung and Blood Institutes NHLBI National Institute of Diabetes and Digestive and Kidney Diseases NIDDK, National Institutes of Health NIH, Bethesda, MD, USA
    Gene Ther 21:148-57. 2014
    ..In summary, 4 Gy TBI is insufficient to permit engraftment of genetically modified HSCs and induce immunological tolerance to transgenes. Our findings should help in the design of conditioning regimens in gene therapy trials...
  18. ncbi request reprint Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells
    D M Bodine
    Clinical Hematology Branch, National Heart, Lung, Blood Institute, Bethesda, MD 20892
    Blood 82:1975-80. 1993
    ..These data provide support for the use of stem cell targeted gene transfer for therapy of ADA deficiency...
  19. ncbi request reprint Sequencing and characterization of A-2 plaque virus: A new member of the Picornaviridae family
    Z Liu
    Hematology Branch, National Institutes of Health, Bethesda, Maryland 20892 1642, USA
    Virology 272:168-76. 2000
    ..These results suggest that A2 virus is a new member of the Picornaviridae but that its pathogenicity in nonhuman primates and association with human disease still need to be determined...