Research Topics
Genomes and Genes | Miral DizdarogluSummaryAffiliation: National Institute of Standards and Technology Country: USA Publications
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Publications
Oxidatively induced DNA damage: Mechanisms, repair and diseaseMiral Dizdaroglu
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA Electronic address
Cancer Lett 327:26-47. 2012..Thus this field deserves more research to contribute to the development of cancer biomarkers, DNA repair inhibitors and treatment approaches to better understand and fight cancer...- Mechanisms of free radical-induced damage to DNAMiral Dizdaroglu
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Free Radic Res 46:382-419. 2012..Further understanding of mechanisms of free radical-induced DNA damage, and cellular repair and biological consequences of DNA damage products will be of outmost importance for disease prevention and treatment... 
Novel substrates of Escherichia coli nth protein and its kinetics for excision of modified bases from DNA damaged by free radicalsM Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
Biochemistry 39:5586-92. 2000..This comparison shows that significant differences exist with respect to substrate specificity and kinetic parameters despite extensive structural conservation among the Nth homologues...- Identification and quantification of DNA repair proteins by liquid chromatography/isotope-dilution tandem mass spectrometry using their fully 15N-labeled analogues as internal standardsMiral Dizdaroglu
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
J Proteome Res 10:3802-13. 2011..The results obtained suggest that the methodology developed would be highly suitable for the positive identification and accurate quantification of DNA repair proteins in vivo as potential biomarkers for cancer and other diseases... - Free radical-induced damage to DNA: mechanisms and measurementMiral Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Free Radic Biol Med 32:1102-15. 2002..This article reviews mechanistic aspects of oxidative damage to DNA and recent developments in the measurement of this type of damage using chromatographic and mass spectrometric techniques... - Substrate specificities and excision kinetics of DNA glycosylases involved in base-excision repair of oxidative DNA damageMiral Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Mutat Res 531:109-26. 2003..This paper reviews the substrate specificities and excision kinetics of DNA glycosylases that have been investigated with the use of gas chromatography/mass spectrometry and DNA substrates with multiple lesions... - Base-excision repair of oxidative DNA damage by DNA glycosylasesMiral Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Mutat Res 591:45-59. 2005..This paper reviews substrate specificities and excision kinetics of DNA glycosylases for removal of pyrimidine- and purine-derived lesions in high-molecular weight DNA... - Substrate specificity and excision kinetics of Escherichia coli endonuclease VIII (Nei) for modified bases in DNA damaged by free radicalsM Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
Biochemistry 40:12150-6. 2001..The present work extends the substrate specificity of Nei and shows the effect of a single mutation in the nei gene on the specificity of Nei... - Identification and quantification of 8,5'-cyclo-2'-deoxy-adenosine in DNA by liquid chromatography/ mass spectrometryM Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Free Radic Biol Med 30:774-84. 2001..The measurement of 8,5'-cyclo-2'-deoxyadenosine by liquid chromatography/mass spectrometry may contribute to the understanding of its biological properties and its role in diseases with defective nucleotide-excision repair... 
Measurement of 8-hydroxy-2'-deoxyguanosine in DNA by high-performance liquid chromatography-mass spectrometry: comparison with measurement by gas chromatography-mass spectrometryM Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Nucleic Acids Res 29:E12. 2001..Taken together, the results unequivocally show that LC/IDMS-SIM is well suited for sensitive and accurate measurement of 8-OH-dGuo in DNA and that both LC/IDMS-SIM and GC/IDMS-SIM can provide similar results...- Formamidopyrimidines in DNA: mechanisms of formation, repair, and biological effectsMiral Dizdaroglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Free Radic Biol Med 45:1610-21. 2008..Our goal is to emphasize the importance of these neglected lesions in many biological and disease processes... - Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosinesPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Biochemistry 49:1053-5. 2010..Further studies aimed at elucidating the mechanism of action of NEIL1 in NER are warranted... - Overexpression and rapid purification of Escherichia coli formamidopyrimidine-DNA glycosylasePrasad Reddy
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
Protein Expr Purif 34:126-33. 2004..Therefore, this method should prove useful for a large number of laboratories and further research on oxidative DNA damage... - Stable isotope-labeling of DNA repair proteins, and their purification and characterizationPrasad T Reddy
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
Protein Expr Purif 78:94-101. 2011..The procedures described in this work may be used for obtaining stable isotope-labeled analogs of other DNA repair proteins for mass spectrometric measurements of these proteins as disease biomarkers... - Lymphoblasts of women with BRCA1 mutations are deficient in cellular repair of 8,5'-Cyclopurine-2'-deoxynucleosides and 8-hydroxy-2'-deoxyguanosineHenry Rodriguez
Chemical Science and Technology Laboratory, and Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
Biochemistry 46:2488-96. 2007..This work suggest that accumulation of these lesions may lead to a high rate of mutations and to deleterious changes in gene expression, increasing breast cancer risk and contributing to breast carcinogenesis... - 8,5'-Cyclopurine-2'-deoxynucleosides in DNA: mechanisms of formation, measurement, repair and biological effectsPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
DNA Repair (Amst) 7:1413-25. 2008..Accumulation of cdA and cdG was observed in tissues in vivo in connection to disease and environmental conditions, suggesting an important role for these lesions in disease processes including carcinogenesis and neuronal death... - Mouse NEIL1 protein is specific for excision of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 4,6-diamino-5-formamidopyrimidine from oxidatively damaged DNAPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
Biochemistry 43:15909-14. 2004..A comparison of the specificity and excision kinetics of mNEIL1 with other DNA glycosylases shows that this enzyme is as efficient as those DNA glycosylases, which specifically remove the formamidopyrimidines from DNA... - Measurement of formamidopyrimidines in DNAPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Free Radic Biol Med 45:1601-9. 2008..This paper reports on the details of this GC/MS methodology... - Mass spectrometric assays for the tandem lesion 8,5'-cyclo-2'-deoxyguanosine in mammalian DNAPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8311, USA
Biochemistry 41:3703-11. 2002..The results showed that both LC/IDMS and GC/IDMS are well suited for the sensitive detection and precise quantification of both (5'R)-8,5'-cdGuo and (5'S)-8,5'-cdGuo in DNA... - Measurement of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in DNA in vivo by liquid chromatography/isotope-dilution tandem mass spectrometryPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Biochem Biophys Res Commun 386:656-60. 2009.... - Chlorella virus pyrimidine dimer glycosylase excises ultraviolet radiation- and hydroxyl radical-induced products 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from DNAPawel Jaruga
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
Photochem Photobiol 75:85-91. 2002..On the other hand, cv-pdg possesses a greater efficiency for the extension of FapyAde than T4-pdg. These two enzymes exhibit different substrate specificities despite substantial structural similarities... - DNA base damage by the antitumor agent 3-amino-1,2,4-benzotriazine 1,4-dioxide (tirapazamine)Mustafa Birincioglu
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8311, USA
J Am Chem Soc 125:11607-15. 2003..Overall, the results indicate that DNA base damage may contribute to the biological effects of tirapazamine in vivo... - Identification and quantification of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNAPawel Jaruga
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Biochem Biophys Res Commun 397:48-52. 2010..This study shows, for the first time, that R-cdA and S-cdA exist in human urine and can be identified and quantified by LC-MS/MS. We propose that R-cdA and S-cdA may be well-suited biomarkers for disease processes such as carcinogenesis... - Accumulation of (5'S)-8,5'-cyclo-2'-deoxyadenosine in organs of Cockayne syndrome complementation group B gene knockout miceGuldal Kirkali
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
DNA Repair (Amst) 8:274-8. 2009..Thus, this study provides, for the first time, the evidence that CSB plays a role in the repair of the DNA helix-distorting tandem lesion S-cdA. Accumulation of unrepaired S-cdA in vivo may contribute to the pathology associated with CS... - DNA damage products (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines as potential biomarkers in human urine for atherosclerosisPawel Jaruga
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States
Biochemistry 51:1822-4. 2012..Our data suggest that R-cdA and S-cdA can be accurately and reproducibly measured in human urine as potential biomarkers of risk and diagnosis for atherosclerosis... - Oxidative DNA damage in polymorphonuclear leukocytes of patients with familial Mediterranean feverGuldal Kirkali
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Free Radic Biol Med 44:386-93. 2008..Future research should deal with prevention of oxidative DNA damage and apoptosis in FMF patients, and also the elucidation of a possible role of DNA repair in this disease... - Identification and quantification of human DNA repair protein NEIL1 by liquid chromatography/isotope-dilution tandem mass spectrometryPrasad T Reddy
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
J Proteome Res 12:1049-61. 2013..These results suggest that the developed assays would be highly suitable for the in vivo positive identification and accurate quantification of hNEIL1 in tissues... - Copper oxide nanoparticle mediated DNA damage in terrestrial plant modelsDonald H Atha
Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, United States
Environ Sci Technol 46:1819-27. 2012..To our knowledge, this is the first evidence of multiple DNA lesion formation and accumulation in plants. These findings provide impetus for future investigations on nanoparticle-mediated DNA damage and repair mechanisms in plants... - Measurement of DNA biomarkers for the safety of tissue-engineered medical products, using artificial skin as a modelHenry Rodriguez
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Tissue Eng 10:1332-45. 2004..More importantly, this exploratory work addresses technological considerations that need to be addressed in order to expedite accurate and useful international reference standards for the emerging tissue-engineering industry... - Complete release of (5'S)-8,5'-cyclo-2'-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesionsPawel Jaruga
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, MD 22777, USA
Nucleic Acids Res 32:e87. 2004..We conclude that (5'S)-cdA can be completely released from DNA by enzymic hydrolysis, and the level of (5'S)-cdA in tissue DNA is comparable to those of other oxidatively induced DNA lesions... - Protective roles of single-wall carbon nanotubes in ultrasonication-induced DNA base damageElijah J Petersen
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Small 9:205-8. 2013..The protective role of SWCNTs observed in this work suggests a contrary view to the general idea that carbon nanotubes have damaging effects on biomolecules... - The oxidatively induced DNA lesions 8,5'-cyclo-2'-deoxyadenosine and 8-hydroxy-2'-deoxyadenosine are strongly resistant to acid-induced hydrolysis of the glycosidic bondJacob A Theruvathu
Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, 5625 Fishers Lane, Room 3S 32, MSC 9412, Bethesda, MD 20952 9412, USA
Mech Ageing Dev 128:494-502. 2007.... - Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1Rachel A Egler
Department of Pediatrics, Section of Hematology Oncology, Children s Hospital of Pittsburgh, Rangos Research Center, 3460 Fifth Ave, USA
Oncogene 24:8038-50. 2005..prdx1-/- mice should be useful in studying the role of oxidative DNA damage in the causation of cancer and its prevention by antioxidants. They should also help in studying the relationship between oncogenes such as c-Myc and DNA damage... - Arabidopsis thaliana Ogg1 protein excises 8-hydroxyguanine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from oxidatively damaged DNA containing multiple lesionsTeresa Morales-Ruiz
Departamento de Genetica, Facultad de Ciencias, Universidad de Cordoba, Cordoba, Spain
Biochemistry 42:3089-95. 2003..The results unequivocally show that AtOgg1 possesses common substrates with other eukaryotic Ogg1 proteins albeit significant differences between their excision kinetics... - The cockayne syndrome group B gene product is involved in cellular repair of 8-hydroxyadenine in DNAJingsheng Tuo
Laboratory of Molecular Gerontology, National Institute on Aging NIH, Baltimore, MD 21224, USA
J Biol Chem 277:30832-7. 2002..These results suggest that the CSB protein contributes to cellular repair of 8-OH-Ade and that the motif VI of the putative helicase domain of CSB is required for this activity... - Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNATapas K Hazra
Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
Proc Natl Acad Sci U S A 99:3523-8. 2002..The tissue-specific levels of NEH1 and OGG1 mRNAs are distinct, and S phase-specific increase in NEH1 at both RNA and protein levels suggests that NEH1 is involved in replication-associated repair of oxidized bases... - Linking uracil base excision repair and 5-fluorouracil toxicity in yeastLauren Seiple
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Nucleic Acids Res 34:140-51. 2006..These findings suggest that new strategies for chemical intervention targeting BER could enhance the effectiveness of this widely used anticancer drug... - Oxidized guanine lesions and hOgg1 activity in lung cancerElizabeth Mambo
Department of Otolaryngology Head and Neck Surgery, Head and Neck Cancer Research Division, The Johns Hopkins University School of Medicine, Baltimore, MD 21205 2196, USA
Oncogene 24:4496-508. 2005..These results provide evidence for defective incision of 8-oxoG in both nuclear and mitochondria of H1650 and H226 lung cancer cell lines. These results may implicate 8-oxoG repair defects in certain lung cancers... - Determination of active site residues in Escherichia coli endonuclease VIIISarah Burgess
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
J Biol Chem 277:2938-44. 2002..These studies provide detailed predictions concerning the active site of endoVIII... - Oxidative DNA damage and disease: induction, repair and significanceMark D Evans
Oxidative Stress Group, Department of Clinical Biochemistry, University of Leicester, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, LE2 7LX, UK
Mutat Res 567:1-61. 2004..However, exact roles remain to be elucidated... - Primary fibroblasts of Cockayne syndrome patients are defective in cellular repair of 8-hydroxyguanine and 8-hydroxyadenine resulting from oxidative stressJingsheng Tuo
Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
FASEB J 17:668-74. 2003..A deficiency in cellular repair of oxidative DNA damage might contribute to developmental defects in CS patients... - Stoichiometric preference in copper-promoted oxidative DNA damage by ochratoxin ARichard A Manderville
Department of Chemistry, Wake Forest University, Winston Salem, NC 27109 7486, USA
J Inorg Biochem 95:87-96. 2003..The implications of these findings with regard to the mutagenicity of OTA are discussed... - New functions of XPC in the protection of human skin cells from oxidative damageMariarosaria D'Errico
Department of Environment and Primary Prevention, Istituto Superiore di Sanita, Rome, Italy
EMBO J 25:4305-15. 2006..The accumulation of endogenous oxidative DNA damage might contribute to increased skin cancer risk and account for internal cancers reported for XP-C patients... - Molecular analysis of base damage clustering associated with a site-specific radiation-induced DNA double-strand breakKamal Datta
Department of Nuclear Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Radiat Res 166:767-81. 2006..These base lesions were 8-hydroxyguanine, 8-hydroxyadenine and 5-hydroxycytosine. Finally, evidence is presented for base damage >24 bp upstream from the (125)I-decay site that may form via a charge migration mechanism... - Reduced repair of 8-hydroxyguanine in the human breast cancer cell line, HCC1937Simon G Nyaga
Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
BMC Cancer 6:297. 2006..The aim of this study was to investigate the ability of HCC1937 and MCF-7 breast cancer cell lines to repair 8-OH-Gua relative to a nonmalignant human mammary epithelial cell line, AG11134... - Biomarkers signal contaminant effects on the organs of English sole (Parophrys vetulus) from Puget SoundDonald C Malins
Biochemical Oncology Program, Pacific Northwest Research Institute, Seattle, Washington 98122, USA
Environ Health Perspect 114:823-9. 2006..The biomarkers described are highly promising for identifying contaminant-induced stresses in fish populations from polluted and reference sites and, in addition, for monitoring the progress of remedial actions... - Human polymorphic variants of the NEIL1 DNA glycosylaseLaura M Roy
Center for Research on Occupational and Environmental Toxicology, Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
J Biol Chem 282:15790-8. 2007..Extrapolation of these data suggests that individuals who are heterozygous for these inactive variant neil1 alleles may be at increased risk for metabolic syndrome... - Accumulation of oxidatively induced DNA damage in human breast cancer cell lines following treatment with hydrogen peroxideSimon G Nyaga
Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
Cell Cycle 6:1472-8. 2007..Furthermore, MCF-7 and HCC1937 cell lines were deficient in the excision repair of all the four lesions studied. These results suggest that oxidatively induced DNA damage and its repair may be critical in the etiology of breast cancer... - Cellular repair of oxidatively induced DNA base lesions is defective in prostate cancer cell lines, PC-3 and DU-145Andrzej R Trzeciak
Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224-6825, USA
Carcinogenesis 25:1359-70. 2004..These data suggest that the malignant phenotype in PC-3 and DU-145 cell lines may be associated with defects in base excision repair and alterations in expression of antioxidant enzymes... - Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymesJingping Hu
Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
J Biol Chem 280:40544-51. 2005..FapyG and FapyA levels were significantly elevated in DNA from the knock-out mice, underscoring the biological role of OGG1 and NTH1 in the repair of these lesions... - Oxidative DNA damage: mechanisms, mutation, and diseaseMarcus S Cooke
Oxidative Stress Group, Department of Clinical Biochemistry, University of Leicester, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, LE2 7LX, UK
FASEB J 17:1195-214. 2003.... - Polyamines stimulate the formation of mutagenic 1,N2-propanodeoxyguanosine adducts from acetaldehydeJacob A Theruvathu
Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, NIH 5625 Fishers Lane, Room 3S32, MSC 9412, Bethesda, MD 20952-9412, USA
Nucleic Acids Res 33:3513-20. 2005..We propose that AA derived from ethanol metabolism is converted to CrA by polyamines in dividing cells, forming Cr-PdG adducts, which may be responsible for the carcinogenicity of alcoholic beverage consumption... - Oxidative changes in the DNA of stroma and epithelium from the female breast: potential implications for breast cancerDonald C Malins
Biochemical Oncology Program, Pacific Northwest Research Institute, Seattle, Washington 98122, USA
Cell Cycle 5:1629-32. 2006..Collectively, the findings imply that the structural changes in DNA described may potentially disrupt normal reciprocal interactions between the cell types, thus increasing breast cancer risk... - Structural alterations in breast stromal and epithelial DNA: the influence of 8,5'-cyclo-2'-deoxyadenosineKatie M Anderson
Biochemical Oncology Program, Pacific Northwest Research Institute, Seattle, Washington, USA
Cell Cycle 5:1240-4. 2006..Initial insight is provided on changes in DNA structure that potentially affect gene expression and increase breast cancer risk...