Leesa J Deterding

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Top-down and bottom-up mass spectrometric characterization of human myoglobin-centered free radicals induced by oxidative damage
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 03, Research Triangle Park, North Carolina 27709, USA
    Anal Chem 79:6236-48. 2007
  2. pmc Global changes in and characterization of specific sites of phosphorylation in mouse and human histone H1 Isoforms upon CDK inhibitor treatment using mass spectrometry
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, DHHS, P O Box 12233, RTP, North Carolina 27709, USA
    J Proteome Res 7:2368-79. 2008
  3. ncbi request reprint Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical
    Leesa J Deterding
    Laboratory of Structural Biology, Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:11600-7. 2004
  4. pmc Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice
    Saurabh Chatterjee
    Free Radical Metabolites Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Free Radic Biol Med 46:454-61. 2009
  5. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
  6. pmc Identification of protein radicals formed in the human neuroglobin-H2O2 reaction using immuno-spin trapping and mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 47:10440-8. 2008
  7. pmc Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 44:893-906. 2008
  8. ncbi request reprint Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:364-9. 2002
  9. pmc Spin scavenging analysis of myoglobin protein-centered radicals using stable nitroxide radicals: characterization of oxoammonium cation-induced modifications
    Olivier M Lardinois
    Laboratories of Pharmacology and Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 22:1034-49. 2009
  10. ncbi request reprint Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:969-76. 2005

Detail Information

Publications30

  1. pmc Top-down and bottom-up mass spectrometric characterization of human myoglobin-centered free radicals induced by oxidative damage
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 03, Research Triangle Park, North Carolina 27709, USA
    Anal Chem 79:6236-48. 2007
    ..Nonetheless, the bottom-up and top-down approaches to protein characterization are complementary rather than competitive approaches with each having its own utility...
  2. pmc Global changes in and characterization of specific sites of phosphorylation in mouse and human histone H1 Isoforms upon CDK inhibitor treatment using mass spectrometry
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, DHHS, P O Box 12233, RTP, North Carolina 27709, USA
    J Proteome Res 7:2368-79. 2008
    ..The specific cyclin-dependent kinase consensus sites of phosphorylation on the histone H1 isoforms that show changes in phosphorylation were also investigated. Three sites of phosphorylation on histone H1.4 isoforms have been identified...
  3. ncbi request reprint Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical
    Leesa J Deterding
    Laboratory of Structural Biology, Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:11600-7. 2004
    ..Because of the pi-pi interaction of Tyr-24 and His-20, the unpaired electron is apparently delocalized on both the tyrosine and histidine residue (pi-pi stacked pair radical)...
  4. pmc Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice
    Saurabh Chatterjee
    Free Radical Metabolites Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Free Radic Biol Med 46:454-61. 2009
    ..This suggests the presence of a possible mechanism for CPB1 activity loss with compensatory protein production...
  5. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..The DMPO adduct formed is initially EPR active but subsequently is oxidized to the stable nitrone, which can then be detected by IST and further characterized by MS and MS/MS...
  6. pmc Identification of protein radicals formed in the human neuroglobin-H2O2 reaction using immuno-spin trapping and mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 47:10440-8. 2008
    ..Together, the data support the argument that wild-type Ngb is protected from attack by H 2O 2 by the coordinated distal His...
  7. pmc Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 44:893-906. 2008
    ..The results demonstrate the power of the new experimental strategy to select DMPO-labeled peptides and identify sites of DMPO covalent attachments...
  8. ncbi request reprint Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:364-9. 2002
    ..Western blot analysis demonstrates that myoglobin forms the predominant radical-derived nitrone adduct in rat heart supernatant...
  9. pmc Spin scavenging analysis of myoglobin protein-centered radicals using stable nitroxide radicals: characterization of oxoammonium cation-induced modifications
    Olivier M Lardinois
    Laboratories of Pharmacology and Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 22:1034-49. 2009
    ..g., by use of 2,4-dinitrophenyl hydrazine). The results reveal the pitfalls inherent in using stable nitroxide radicals such as TEMPOL to identify sites of radical formation on hemoproteins...
  10. ncbi request reprint Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:969-76. 2005
    ..Interestingly, formation of the myoglobin dimer, which is known to be formed primarily by cross-linkage of tyrosine-151, was inhibited by the addition of DMPO...
  11. pmc Procainamide, but not N-acetylprocainamide, induces protein free radical formation on myeloperoxidase: a potential mechanism of agranulocytosis
    Arno G Siraki
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Dr, Research Triangle Park, NC 27709, USA
    Chem Res Toxicol 21:1143-53. 2008
    ..We propose that drug-induced free radical formation on MPO may play a role in the origin of agranulocytosis...
  12. pmc Mass spectrometric identification of oxidative modifications of tryptophan residues in proteins: chemical artifact or post-translational modification?
    Irina Perdivara
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, Research Triangle Park, North Carolina 27709, USA
    J Am Soc Mass Spectrom 21:1114-7. 2010
    ..These results indicate that Trp oxidation may occur as an artifact in proteins separated by SDS-PAGE, and their presence should be carefully interpreted, especially when gel electrophoretic separation methods are employed...
  13. ncbi request reprint Electron transfer between a tyrosyl radical and a cysteine residue in hemoproteins: spin trapping analysis
    Suchandra Bhattacharjee
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Am Chem Soc 129:13493-501. 2007
    ..This complex phenomenon of electron transfer from one radical to another has been investigated in proteins by IST, ESR, and MS...
  14. pmc Elucidation of O-glycosylation structures of the beta-amyloid precursor protein by liquid chromatography-mass spectrometry using electron transfer dissociation and collision induced dissociation
    Irina Perdivara
    National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Proteome Res 8:631-42. 2009
    ..Thus, the combination of the CID and ETD techniques in LC-MS is shown here, as a powerful tool for de novo identification of O-glycosylations at unknown modification sites in proteins...
  15. ncbi request reprint Protein oxidation of cytochrome C by reactive halogen species enhances its peroxidase activity
    Yeong Renn Chen
    Laboratory of Pharmacology and Chemistry and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:29781-91. 2002
    ....
  16. pmc Site-specific carboxypeptidase B1 tyrosine nitration and pathophysiological implications following its physical association with nitric oxide synthase-3 in experimental sepsis
    Saurabh Chatterjee
    Free Radical Metabolism Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Immunol 183:4055-66. 2009
    ..These results open up new avenues for the investigation of several enzymes involved in inflammation and their site-specific oxidative modifications by protein-protein interactions as well as their role in sepsis...
  17. pmc Comparison of metal and metal oxide media for phosphopeptide enrichment prior to mass spectrometric analyses
    Matthew B Gates
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Am Soc Mass Spectrom 21:1649-59. 2010
    ..These methods should aid in the investigation of proteins post-translationally modified with phosphate, especially those present at low concentrations as was demonstrated by successful enrichment at the femtomole level...
  18. pmc Targeted oxidation of Torpedo californica acetylcholinesterase by singlet oxygen: identification of N-formylkynurenine tryptophan derivatives within the active-site gorge of its complex with the photosensitizer methylene blue
    Mathilde M Triquigneaux
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, DHHS, PO Box 12233 MD F0 03, Research Triangle Park, NC 27709, USA
    Biochem J 448:83-91. 2012
    ....
  19. ncbi request reprint Understanding global changes in histone H1 phosphorylation using mass spectrometry
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Methods 33:53-8. 2004
    ..The continued developments in the area of mass spectrometry should provide new insights into not only the function of proteins but also into the basic regulatory mechanisms that control cellular functions...
  20. pmc Simplified synthesis of isotopically labeled 5,5-dimethyl-pyrroline N-oxide
    Fabian Leinisch
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Molecules 16:8428-36. 2011
    ..This facile, straightforward process allows synthesizing isotopically labeled DMPO analogs in a 4-step reaction without special equipment...
  21. pmc Mass spectrometric determination of IgG subclass-specific glycosylation profiles in siblings discordant for myositis syndromes
    Irina Perdivara
    Laboratory of Structural Biology, NIH DHHS, Research Triangle Park, North Carolina 27709, USA
    J Proteome Res 10:2969-78. 2011
    ....
  22. pmc Identification of protein-derived tyrosyl radical in the reaction of cytochrome c and hydrogen peroxide: characterization by ESR spin-trapping, HPLC and MS
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, U S A
    Biochem J 363:281-8. 2002
    ..We conclude that the cytochrome c-derived tyrosyl radical was trapped by MNP, leading to a persistent radical adduct at the C-3/C-5 positions of the tyrosine phenyl ring...
  23. pmc Diversity in penaeidin antimicrobial peptide form and function
    Brandon J Cuthbertson
    Laboratory of Signal Transduction, NIH NIEHS, P O Box 12233 MD F3 04, 111 TW Alexander Drive, Research Triangle Park, NC 27709 2233, USA
    Dev Comp Immunol 32:167-81. 2008
    ....
  24. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
    ....
  25. pmc Characterization of glycopeptides from HIV-I(SF2) gp120 by liquid chromatography mass spectrometry
    Jenny M Cutalo
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences National Institutes of Health Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Am Soc Mass Spectrom 15:1545-55. 2004
    ..In addition, a peptide containing two consensus glycosylation sites, which had previously been determined to contain complex glycans, was also determined to be high mannose as well...
  26. ncbi request reprint Effect of nonaqueous buffer modifiers on the capillary electrophoresis-mass spectrometry analysis of peptides
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Capill Electrophor Microchip Technol 8:11-8. 2003
    ..From our data, the addition of acetonitrile to the CE buffer appears to be the most compatible with mass spectrometry due to shorter analysis times and increased sensitivities in comparison to methanol and N-methylformamide...
  27. pmc Sulfite-mediated oxidation of myeloperoxidase to a free radical: immuno-spin trapping detection in human neutrophils
    Kalina Ranguelova
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 60:98-106. 2013
    ..Our present study demonstrates that myeloperoxidase initiates (bi)sulfite oxidation leading to MPO radical damage, possibly leading to (bi)sulfite-exacerbated allergic reactions...
  28. pmc HMGB1 is a cofactor in mammalian base excision repair
    Rajendra Prasad
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Cell 27:829-41. 2007
    ..The results suggest HMGB1 is a BER cofactor capable of modulating BER capacity in cells...
  29. ncbi request reprint Separation and characterization of human high-density apolipoproteins using a nonaqueous modifier in capillary electrophoresis-mass spectrometry
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Electrophoresis 23:2296-305. 2002
    ..These results demonstrate that CE in combination with MS is a promising technique for screening and characterizing isomers of plasma apolipoproteins...
  30. pmc Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin
    Heping Cao
    US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Expert Rev Proteomics 4:711-26. 2007
    ..This information should provide the molecular basis for future investigation of TTP's regulatory functions in controlling proinflammatory cytokines...