Research Topics
| S A DesaiSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Solute Restriction Reveals an Essential Role for clag3-Associated Channels in Malaria Parasite Nutrient AcquisitionAjay D Pillai
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Room 3W 01, Rockville, MD 20852
Mol Pharmacol 82:1104-14. 2012..These findings indicate that PSAC functions in nutrient acquisition for intracellular parasites. Although key questions regarding the channel and its biological role remain, antimalarial drug development targeting PSAC should be pursued...- Ion and nutrient uptake by malaria parasite-infected erythrocytesSanjay A Desai
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20894, USA
Cell Microbiol 14:1003-9. 2012..In either case, gene identification should advance our understanding of parasite biology and may lead to new therapeutics... - A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplatesBrian Lyko
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
Malar J 11:124. 2012..The parasite's slow replication rate combined with laborious methods for identification of positive wells has limited these studies. A new high-throughput method for detecting growth without compromising parasite viability is reported... - Open and closed states of the plasmodial surface anion channelSanjay A Desai
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Nanomedicine 1:58-66. 2005..Detailed study of its gating properties have been complicated by the technical difficulty of obtaining single PSAC recordings, its small conductance, and various sources of error important for fast-flickering channels... - Targeting ion channels of Plasmodium falciparum-infected human erythrocytes for antimalarial developmentSanjay A Desai
Bldg 4, Room 126, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA
Curr Drug Targets Infect Disord 4:79-86. 2004..If so, they present novel opportunities for identification of ion channel inhibitors that may be useful antimalarial compounds... - Plasmodial surface anion channel-independent phloridzin resistance in Plasmodium falciparumSanjay A Desai
Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 280:16861-7. 2005..Failure to induce mutations in PSAC activity is consistent with a highly constrained channel protein less susceptible to resistance mutations; whether this protein is parasite- or host-encoded remains to be determined... - A voltage-dependent channel involved in nutrient uptake by red blood cells infected with the malaria parasiteS A Desai
The Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 406:1001-5. 2000..The surface location of this new channel and its permeability to organic solutes needed for parasite growth indicate that it may have a primary role in a sequential diffusive pathway for parasite nutrient acquisition... - A two-compartment model of osmotic lysis in Plasmodium falciparum-infected erythrocytesMarissa A Wagner
Molecular Physiology Unit, Laboratory of Malaria and Vector Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 84:116-23. 2003..Our model and light scattering assay also revealed Michaelian kinetics for inhibition of PESAC by furosemide, suggesting a 1:1 stoichiometry for their interaction... - The plasmodial surface anion channel is functionally conserved in divergent malaria parasitesGodfrey Lisk
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892, USA
Eukaryot Cell 4:2153-9. 2005..Together with PSAC's exposed location on the host cell surface and its central role in transport changes after infection, this conservation supports development of antimalarial drugs against the PSAC family... - Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compoundsGodfrey Lisk
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
Biochim Biophys Acta 1798:1679-88. 2010..These findings are consistent with a shared channel that mediates uptake of ions, nutrients and toxins. This channel's gating and selectivity properties can be modified in response to in vitro selective pressure... - Extracellular lysines on the plasmodial surface anion channel involved in Na+ exclusionJamieson V Cohn
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Mol Biochem Parasitol 132:27-34. 2003..When combined with the effects of pH on permeation, these findings implicate a combination of cation repulsion by pore mouth charges and a weak binding site for permeant solutes in PSAC's broad selectivity yet effective exclusion of Na+... - Plasmodium falciparum likely encodes the principal anion channel on infected human erythrocytesAbdulnaser Alkhalil
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Blood 104:4279-86. 2004..The absence of known eukaryotic ion channel homologues in the completed P falciparum genome suggests a novel channel gene, and substantiates PSAC as a target for antimalarial development... - Specific inhibition of the plasmodial surface anion channel by dantroleneGodfrey Lisk
Laboratory of Malaria and Vector Research, NIAID/NIH, Room 3W-01, 12735 Twinbrook Parkway, Rockville, Maryland 20852-8132, USA
Eukaryot Cell 5:1882-93. 2006..In addition to the potential therapeutic applications of its derivatives, dantrolene's specificity and its defined mechanism of action on PSAC make it a useful tool for transport studies of infected erythrocytes... - Complex inheritance of the plasmodial surface anion channel in a Plasmodium falciparum genetic crossAbdulnaser Alkhalil
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
Mol Microbiol 72:459-69. 2009..PSAC may be encoded by multiple parasite genes (e.g. a multi-gene family or multiple genes that encode distinct channel subunits) or a single polymorphic gene under strong selective pressure... - Solute-inhibitor interactions in the plasmodial surface anion channel reveal complexities in the transport processGodfrey Lisk
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Mol Pharmacol 71:1241-50. 2007..We propose a possible model that also may help explain the unique selectivity properties of PSAC... - Changes in the plasmodial surface anion channel reduce leupeptin uptake and can confer drug resistance in Plasmodium falciparum-infected erythrocytesGodfrey Lisk
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892, USA
Antimicrob Agents Chemother 52:2346-54. 2008..Drug development based on such compounds should proceed cautiously in light of possible resistance development though the selection of PSAC mutants... - A cell-based high-throughput screen validates the plasmodial surface anion channel as an antimalarial targetAjay D Pillai
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852 8132, USA
Mol Pharmacol 77:724-33. 2010..These new inhibitors should be important research tools and may be starting points for much-needed antimalarial drugs... - A nutrient-permeable channel on the intraerythrocytic malaria parasiteS A Desai
National Institutes of Health National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases, Bethesda, MD 20814, USA
Novartis Found Symp 226:89-95; discussion 95-8. 1999..These experiments, together with the patch clamp findings, suggest a high capacity molecular sieve that allows the parasite to acquire soluble nutrients from erythrocyte cytosol... - A blasticidin S-resistant Plasmodium falciparum mutant with a defective plasmodial surface anion channelDavid A Hill
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20852, USA
Proc Natl Acad Sci U S A 104:1063-8. 2007..These findings provide solid evidence for the uptake of diverse solutes via PSAC and implicate one or more parasite genes in expression of this channel... - Two distinct mechanisms of transport through the plasmodial surface anion channelAbdullah A B Bokhari
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852 8132, USA
J Membr Biol 226:27-34. 2008..In contrast to most other broad-permeability channels, selectivity in PSAC appears to be complex and cannot be adequately explained by simple models that invoke sieving through rigid, noninteracting pores... - Babesia and plasmodia increase host erythrocyte permeability through distinct mechanismsAbdulnaser Alkhalil
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD 20892, USA
Cell Microbiol 9:851-60. 2007..divergens infection. While both babesia and plasmodia increase host erythrocyte permeability to a diverse collection of organic solutes, they utilize fundamentally different mechanisms... - Malaria parasites are rapidly killed by dantrolene derivatives specific for the plasmodial surface anion channelMyungsa Kang
The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Mol Pharmacol 68:34-40. 2005..Because, as a class, these derivatives fulfil the requirements for drug leads and can be studied with simple screening technology, more extensive medicinal chemistry is warranted to explore antimalarial development... - Improved perfusion conditions for patch-clamp recordings on human erythrocytesGodfrey Lisk
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Biophys Res Commun 347:158-65. 2006..Electrophysiological recordings then faithfully reproduced several findings made with more traditional methods. The new perfusion chamber should also be useful for patch-clamp recordings on blood cells, protoplasts, and organelles... - A complex of three related membrane proteins is conserved on malarial merozoitesKempaiah Rayavara
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Biochem Parasitol 167:135-43. 2009..Because all three genes are highly conserved in apicomplexan parasites, the complex formed by their encoded proteins likely serves an essential role for invasive merozoites... - Electrophysiological studies of malaria parasite-infected erythrocytes: current statusHenry M Staines
Centre for Infection, Division of Cellular and Molecular Medicine, St George s, University of London, London SW17 0RE, UK
Int J Parasitol 37:475-82. 2007..In this article, the various models for altered transport are reviewed, together with the areas of consensus in the field and those that require a better understanding... - Permeant but not impermeant divalent cations enhance activation of nondesensitizing alpha(7) nicotinic receptorsDonnie Eddins
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Am J Physiol Cell Physiol 282:C796-804. 2002..The difference between permeant and impermeant divalent cations suggests that sites within the pore are important for modulation by divalent cations...