S A Desai

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint Why do malaria parasites increase host erythrocyte permeability?
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA Electronic address
    Trends Parasitol 30:151-9. 2014
  2. pmc Insights gained from P. falciparum cultivation in modified media
    Sanjay A Desai
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    ScientificWorldJournal 2013:363505. 2013
  3. pmc Solute restriction reveals an essential role for clag3-associated channels in malaria parasite nutrient acquisition
    Ajay D Pillai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Mol Pharmacol 82:1104-14. 2012
  4. pmc Ion and nutrient uptake by malaria parasite-infected erythrocytes
    Sanjay A Desai
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20894, USA
    Cell Microbiol 14:1003-9. 2012
  5. pmc A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates
    Brian Lyko
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Malar J 11:124. 2012
  6. ncbi request reprint Plasmodial surface anion channel-independent phloridzin resistance in Plasmodium falciparum
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:16861-7. 2005
  7. ncbi request reprint Targeting ion channels of Plasmodium falciparum-infected human erythrocytes for antimalarial development
    Sanjay A Desai
    Bldg 4, Room 126, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA
    Curr Drug Targets Infect Disord 4:79-86. 2004
  8. ncbi request reprint A voltage-dependent channel involved in nutrient uptake by red blood cells infected with the malaria parasite
    S A Desai
    The Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 406:1001-5. 2000
  9. ncbi request reprint Open and closed states of the plasmodial surface anion channel
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Nanomedicine 1:58-66. 2005
  10. pmc A two-compartment model of osmotic lysis in Plasmodium falciparum-infected erythrocytes
    Marissa A Wagner
    Molecular Physiology Unit, Laboratory of Malaria and Vector Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 84:116-23. 2003

Collaborators

Detail Information

Publications28

  1. doi request reprint Why do malaria parasites increase host erythrocyte permeability?
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA Electronic address
    Trends Parasitol 30:151-9. 2014
    ..Because both channel activity and the associated clag3 genes are strictly conserved in malaria parasites, channel-mediated permeability is an attractive target for development of new therapies. ..
  2. pmc Insights gained from P. falciparum cultivation in modified media
    Sanjay A Desai
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    ScientificWorldJournal 2013:363505. 2013
    ..The findings are relevant to understanding in vivo parasite phenotypes and the prioritization of antimalarial targets. ..
  3. pmc Solute restriction reveals an essential role for clag3-associated channels in malaria parasite nutrient acquisition
    Ajay D Pillai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Mol Pharmacol 82:1104-14. 2012
    ..These findings indicate that PSAC functions in nutrient acquisition for intracellular parasites. Although key questions regarding the channel and its biological role remain, antimalarial drug development targeting PSAC should be pursued...
  4. pmc Ion and nutrient uptake by malaria parasite-infected erythrocytes
    Sanjay A Desai
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20894, USA
    Cell Microbiol 14:1003-9. 2012
    ..In either case, gene identification should advance our understanding of parasite biology and may lead to new therapeutics...
  5. pmc A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates
    Brian Lyko
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    Malar J 11:124. 2012
    ..The parasite's slow replication rate combined with laborious methods for identification of positive wells has limited these studies. A new high-throughput method for detecting growth without compromising parasite viability is reported...
  6. ncbi request reprint Plasmodial surface anion channel-independent phloridzin resistance in Plasmodium falciparum
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:16861-7. 2005
    ..Failure to induce mutations in PSAC activity is consistent with a highly constrained channel protein less susceptible to resistance mutations; whether this protein is parasite- or host-encoded remains to be determined...
  7. ncbi request reprint Targeting ion channels of Plasmodium falciparum-infected human erythrocytes for antimalarial development
    Sanjay A Desai
    Bldg 4, Room 126, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA
    Curr Drug Targets Infect Disord 4:79-86. 2004
    ..If so, they present novel opportunities for identification of ion channel inhibitors that may be useful antimalarial compounds...
  8. ncbi request reprint A voltage-dependent channel involved in nutrient uptake by red blood cells infected with the malaria parasite
    S A Desai
    The Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 406:1001-5. 2000
    ..The surface location of this new channel and its permeability to organic solutes needed for parasite growth indicate that it may have a primary role in a sequential diffusive pathway for parasite nutrient acquisition...
  9. ncbi request reprint Open and closed states of the plasmodial surface anion channel
    Sanjay A Desai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Nanomedicine 1:58-66. 2005
    ..Detailed study of its gating properties have been complicated by the technical difficulty of obtaining single PSAC recordings, its small conductance, and various sources of error important for fast-flickering channels...
  10. pmc A two-compartment model of osmotic lysis in Plasmodium falciparum-infected erythrocytes
    Marissa A Wagner
    Molecular Physiology Unit, Laboratory of Malaria and Vector Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 84:116-23. 2003
    ..Our model and light scattering assay also revealed Michaelian kinetics for inhibition of PESAC by furosemide, suggesting a 1:1 stoichiometry for their interaction...
  11. pmc The plasmodial surface anion channel is functionally conserved in divergent malaria parasites
    Godfrey Lisk
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892, USA
    Eukaryot Cell 4:2153-9. 2005
    ..Together with PSAC's exposed location on the host cell surface and its central role in transport changes after infection, this conservation supports development of antimalarial drugs against the PSAC family...
  12. ncbi request reprint Extracellular lysines on the plasmodial surface anion channel involved in Na+ exclusion
    Jamieson V Cohn
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Mol Biochem Parasitol 132:27-34. 2003
    ..When combined with the effects of pH on permeation, these findings implicate a combination of cation repulsion by pore mouth charges and a weak binding site for permeant solutes in PSAC's broad selectivity yet effective exclusion of Na+...
  13. pmc Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compounds
    Godfrey Lisk
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    Biochim Biophys Acta 1798:1679-88. 2010
    ..These findings are consistent with a shared channel that mediates uptake of ions, nutrients and toxins. This channel's gating and selectivity properties can be modified in response to in vitro selective pressure...
  14. pmc Complex inheritance of the plasmodial surface anion channel in a Plasmodium falciparum genetic cross
    Abdulnaser Alkhalil
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852, USA
    Mol Microbiol 72:459-69. 2009
    ..PSAC may be encoded by multiple parasite genes (e.g. a multi-gene family or multiple genes that encode distinct channel subunits) or a single polymorphic gene under strong selective pressure...
  15. pmc Changes in the plasmodial surface anion channel reduce leupeptin uptake and can confer drug resistance in Plasmodium falciparum-infected erythrocytes
    Godfrey Lisk
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892, USA
    Antimicrob Agents Chemother 52:2346-54. 2008
    ..Drug development based on such compounds should proceed cautiously in light of possible resistance development though the selection of PSAC mutants...
  16. ncbi request reprint Solute-inhibitor interactions in the plasmodial surface anion channel reveal complexities in the transport process
    Godfrey Lisk
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Mol Pharmacol 71:1241-50. 2007
    ..We propose a possible model that also may help explain the unique selectivity properties of PSAC...
  17. ncbi request reprint Plasmodium falciparum likely encodes the principal anion channel on infected human erythrocytes
    Abdulnaser Alkhalil
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 104:4279-86. 2004
    ..The absence of known eukaryotic ion channel homologues in the completed P falciparum genome suggests a novel channel gene, and substantiates PSAC as a target for antimalarial development...
  18. pmc Specific inhibition of the plasmodial surface anion channel by dantrolene
    Godfrey Lisk
    Laboratory of Malaria and Vector Research, NIAID NIH, Room 3W 01, 12735 Twinbrook Parkway, Rockville, Maryland 20852 8132, USA
    Eukaryot Cell 5:1882-93. 2006
    ..In addition to the potential therapeutic applications of its derivatives, dantrolene's specificity and its defined mechanism of action on PSAC make it a useful tool for transport studies of infected erythrocytes...
  19. pmc A cell-based high-throughput screen validates the plasmodial surface anion channel as an antimalarial target
    Ajay D Pillai
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852 8132, USA
    Mol Pharmacol 77:724-33. 2010
    ..These new inhibitors should be important research tools and may be starting points for much-needed antimalarial drugs...
  20. ncbi request reprint A nutrient-permeable channel on the intraerythrocytic malaria parasite
    S A Desai
    National Institutes of Health National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases, Bethesda, MD 20814, USA
    Novartis Found Symp 226:89-95; discussion 95-8. 1999
    ..These experiments, together with the patch clamp findings, suggest a high capacity molecular sieve that allows the parasite to acquire soluble nutrients from erythrocyte cytosol...
  21. pmc A blasticidin S-resistant Plasmodium falciparum mutant with a defective plasmodial surface anion channel
    David A Hill
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20852, USA
    Proc Natl Acad Sci U S A 104:1063-8. 2007
    ..These findings provide solid evidence for the uptake of diverse solutes via PSAC and implicate one or more parasite genes in expression of this channel...
  22. ncbi request reprint Babesia and plasmodia increase host erythrocyte permeability through distinct mechanisms
    Abdulnaser Alkhalil
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD 20892, USA
    Cell Microbiol 9:851-60. 2007
    ..divergens infection. While both babesia and plasmodia increase host erythrocyte permeability to a diverse collection of organic solutes, they utilize fundamentally different mechanisms...
  23. pmc Two distinct mechanisms of transport through the plasmodial surface anion channel
    Abdullah A B Bokhari
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD 20852 8132, USA
    J Membr Biol 226:27-34. 2008
    ..In contrast to most other broad-permeability channels, selectivity in PSAC appears to be complex and cannot be adequately explained by simple models that invoke sieving through rigid, noninteracting pores...
  24. ncbi request reprint Malaria parasites are rapidly killed by dantrolene derivatives specific for the plasmodial surface anion channel
    Myungsa Kang
    The Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Mol Pharmacol 68:34-40. 2005
    ..Because, as a class, these derivatives fulfil the requirements for drug leads and can be studied with simple screening technology, more extensive medicinal chemistry is warranted to explore antimalarial development...
  25. pmc Improved perfusion conditions for patch-clamp recordings on human erythrocytes
    Godfrey Lisk
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 347:158-65. 2006
    ..Electrophysiological recordings then faithfully reproduced several findings made with more traditional methods. The new perfusion chamber should also be useful for patch-clamp recordings on blood cells, protoplasts, and organelles...
  26. pmc A complex of three related membrane proteins is conserved on malarial merozoites
    Kempaiah Rayavara
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biochem Parasitol 167:135-43. 2009
    ..Because all three genes are highly conserved in apicomplexan parasites, the complex formed by their encoded proteins likely serves an essential role for invasive merozoites...
  27. pmc Electrophysiological studies of malaria parasite-infected erythrocytes: current status
    Henry M Staines
    Centre for Infection, Division of Cellular and Molecular Medicine, St George s, University of London, London SW17 0RE, UK
    Int J Parasitol 37:475-82. 2007
    ..In this article, the various models for altered transport are reviewed, together with the areas of consensus in the field and those that require a better understanding...
  28. ncbi request reprint Permeant but not impermeant divalent cations enhance activation of nondesensitizing alpha(7) nicotinic receptors
    Donnie Eddins
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Am J Physiol Cell Physiol 282:C796-804. 2002
    ..The difference between permeant and impermeant divalent cations suggests that sites within the pore are important for modulation by divalent cations...