Melvin L DePamphilis

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Cell cycle dependent regulation of the origin recognition complex
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, Building 6, Room 3A 15, 9000 Rockville Pike, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Cell Cycle 4:70-9. 2005
  2. pmc Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidy
    Zakir Ullah
    National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Cell Div 4:10. 2009
  3. ncbi request reprint Regulating the licensing of DNA replication origins in metazoa
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Curr Opin Cell Biol 18:231-9. 2006
  4. ncbi request reprint The 'ORC cycle': a novel pathway for regulating eukaryotic DNA replication
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, Building 6 416, 9000 Rockville Pike, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Gene 310:1-15. 2003
  5. pmc Spotlight on geminin
    Melvin L DePamphilis
    Breast Cancer Res 13:109. 2011
  6. pmc The acrosomal protein Dickkopf-like 1 (DKKL1) facilitates sperm penetration of the zona pellucida
    Matthew J Kohn
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Fertil Steril 93:1533-7. 2010
  7. pmc The acrosomal protein Dickkopf-like 1 (DKKL1) is not essential for fertility
    Kotaro J Kaneko
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Fertil Steril 93:1526-32. 2010
  8. pmc The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo
    Kohji Noguchi
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 25:5372-82. 2006
  9. ncbi request reprint Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development
    Rieko Yagi
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892 2753, USA
    Development 134:3827-36. 2007
  10. pmc Ubiquitylation, phosphorylation and Orc2 modulate the subcellular location of Orc1 and prevent it from inducing apoptosis
    Tapas Saha
    National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    J Cell Sci 119:1371-82. 2006

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Cell cycle dependent regulation of the origin recognition complex
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, Building 6, Room 3A 15, 9000 Rockville Pike, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Cell Cycle 4:70-9. 2005
    ..Thus, this "ORC cycle" is the premier step in preventing rereplication of DNA during a single cell division cycle...
  2. pmc Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidy
    Zakir Ullah
    National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Cell Div 4:10. 2009
    ..Given the fact that p21 and p57 are unique to mammals, the question arises as to whether one or both of these proteins are responsible for the induction and maintenance of polyploidy during mammalian development...
  3. ncbi request reprint Regulating the licensing of DNA replication origins in metazoa
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Curr Opin Cell Biol 18:231-9. 2006
    ....
  4. ncbi request reprint The 'ORC cycle': a novel pathway for regulating eukaryotic DNA replication
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, Building 6 416, 9000 Rockville Pike, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Gene 310:1-15. 2003
    ....
  5. pmc Spotlight on geminin
    Melvin L DePamphilis
    Breast Cancer Res 13:109. 2011
    ..Remarkably, either depletion of Geminin or over-expression of Geminin inhibits the action of TopoII╬▒, thereby making Geminin an excellent target for cancer chemotherapy...
  6. pmc The acrosomal protein Dickkopf-like 1 (DKKL1) facilitates sperm penetration of the zona pellucida
    Matthew J Kohn
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Fertil Steril 93:1533-7. 2010
    ..To determine the role of Dkkl1 in mouse development, viability, and fertility...
  7. pmc The acrosomal protein Dickkopf-like 1 (DKKL1) is not essential for fertility
    Kotaro J Kaneko
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Fertil Steril 93:1526-32. 2010
    ..To determine the role of Dkkl1 on mouse development, viability, and fertility...
  8. pmc The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivo
    Kohji Noguchi
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 25:5372-82. 2006
    ..Thus, the BAH domain in human Orc1 facilitates its ability to activate replication origins in vivo by promoting association of ORC with chromatin...
  9. ncbi request reprint Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development
    Rieko Yagi
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892 2753, USA
    Development 134:3827-36. 2007
    ..Thus, Tead4 is the earliest gene shown to be uniquely required for specification of the trophectoderm lineage...
  10. pmc Ubiquitylation, phosphorylation and Orc2 modulate the subcellular location of Orc1 and prevent it from inducing apoptosis
    Tapas Saha
    National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    J Cell Sci 119:1371-82. 2006
    ..These results provide direct evidence in support of the regulatory role proposed for Orc1, and suggest that aberrant DNA replication during mammalian development could result in apoptosis through the appearance of 'unmodified' Orc1...
  11. pmc Transcription factor TEAD2 is involved in neural tube closure
    Kotaro J Kaneko
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Genesis 45:577-87. 2007
    ..These results reveal a maternal genetic contribution to neural tube closure, and suggest that Tead2-deficient mice provide a model for anencephaly, a common human birth defect that can be prevented by folic acid...
  12. pmc Xenopus origin recognition complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORC
    Daochun Kong
    National Institute of Child Health and Human Development, Building 6 416, 9000 Rockville Pike, National Institutes of Health, Bethesda, MD 20892 2753, USA
    EMBO J 22:3441-50. 2003
    ..These results reveal that XlORC initiates DNA replication preferentially at the same or similar sites to those targeted in S.pombe...
  13. doi request reprint High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit them
    Chrissie Y Lee
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, United States
    Methods 57:234-48. 2012
    ....
  14. pmc Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin
    Wei Hsin Sun
    National Institute of Child Health and Human Development, Building 6 416, National Institutes of Health, Bethesda, MD 20892 2753, USA
    EMBO J 21:1437-46. 2002
    ..These results demonstrate a programmed release of XlORC from somatic cell chromatin as it enters S phase, consistent with the proposed role for ORC in preventing re-initiation of DNA replication during S phase...
  15. ncbi request reprint Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex
    Ilian Radichev
    NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    J Biol Chem 281:23264-73. 2006
    ..Because this suppression required only the ORC assembly and NLS domains, these domains appear to constitute the functional domain of Orc2...
  16. pmc An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cells
    Wenge Zhu
    National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 2753, USA
    Mol Cancer Res 9:294-310. 2011
    ..Thus, this assay provides a new approach to the discovery of compounds useful for investigating the regulation of genome duplication and for the treatment of cancer...
  17. pmc The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cells
    Jung Min Park
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 31 Center Drive, Bldg 31, Rm 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 26:1445-51. 2006
    ..The results indicate that in contrast to the situation in yeasts, La is essential in mammals and is one of a limited number of genes required as early as the development of the ICM...
  18. pmc Checkpoint kinase 1 prevents cell cycle exit linked to terminal cell differentiation
    Zakir Ullah
    National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 31:4129-43. 2011
    ..Thus, CHK1 is part of a "G2 restriction point" that prevents premature cell cycle exit in cells programmed for terminal differentiation, a role that CHK2 cannot play...
  19. pmc Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity
    Zakir Ullah
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 22:3024-36. 2008
    ..Expression of p57 in TG cells was restricted to G-phase nuclei to allow CDK activation of S phase. Thus, endoreduplication in TS cells is triggered by p57 inhibition of CDK1 with concomitant suppression of the DNA damage response by p21...
  20. pmc Assembly of the human origin recognition complex occurs through independent nuclear localization of its components
    Soma Ghosh
    NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    J Biol Chem 286:23831-41. 2011
    ..These and other results support a model in which Orc6, Orc1, and ORC(2-5) are transported independently to the nucleus where they can either assemble into ORC(1-6) or function individually...
  21. pmc Developmentally programmed endoreduplication in animals
    Zakir Ullah
    Genomics of Differentiation Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Cycle 8:1501-9. 2009
    ..The critical step, however, appears to be switching from a CDK-dependent form of the anaphase promoting complex (APC) to one that functions only in the absence of CDK activity...
  22. ncbi request reprint Methods for detecting cells in S phase
    Wei Hsin Sun
    National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 241:37-53. 2004
  23. pmc Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycle
    Cong jun Li
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Mol Cell Biol 22:105-16. 2002
    ....
  24. pmc DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis
    Matthew J Kohn
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Mol Reprod Dev 71:516-22. 2005
    ..These results identify a member of the Dickkopf family as a novel acrosomal protein that may be involved in acrosome assembly or function, a unique role for a secreted signaling molecule...
  25. pmc Site-specific ORC binding, pre-replication complex assembly and DNA synthesis at Schizosaccharomyces pombe replication origins
    Daochun Kong
    National Institute of Child Health and Human Development, Building 6, Room 416, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    EMBO J 21:5567-76. 2002
    ..Schizosaccharomyces pombe may be an appropriate paradigm for the complex origins found in the metazoa...
  26. ncbi request reprint Eukaryotic DNA replication origins: reconciling disparate data
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, Building 6, Room 416, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Cell 114:274-5. 2003
  27. pmc Selective killing of cancer cells by suppression of geminin activity
    Wenge Zhu
    National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892 2753, USA
    Cancer Res 69:4870-7. 2009
    ..These results show that inhibition of geminin activity could be used to selectively kill cancer cells without harming other cells...
  28. pmc Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis
    Robert V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 6 Center Dr, Rm 416, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 24:10894-904. 2004
    ..The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis...
  29. pmc "The Octet": Eight Protein Kinases that Control Mammalian DNA Replication
    Melvin L DePamphilis
    Program in Genomics of Differentiation, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD, USA
    Front Physiol 3:368. 2012
    ....
  30. pmc DNA methylation may restrict but does not determine differential gene expression at the Sgy/Tead2 locus during mouse development
    Kotaro J Kaneko
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Mol Cell Biol 24:1968-82. 2004
    ....
  31. pmc TEAD4 establishes the energy homeostasis essential for blastocoel formation
    Kotaro J Kaneko
    National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Development 140:3680-90. 2013
    ..These results reveal that TEAD4 plays a crucial role in maintaining energy homeostasis during preimplantation development. ..
  32. pmc Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosis
    Cong jun Li
    Growth Biology Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Mol Cell Biol 24:5875-86. 2004
    ..These results reveal that the same cyclin-dependent protein kinase that initiates mitosis in mammalian cells also concomitantly inhibits assembly of functional ORC-chromatin sites...