Research Topics
Genomes and Genes
| Melvin L DePamphilisSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
Cell cycle dependent regulation of the origin recognition complexMelvin L DePamphilis
National Institute of Child Health and Human Development, Building 6, Room 3A 15, 9000 Rockville Pike, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Cell Cycle 4:70-9. 2005..Thus, this "ORC cycle" is the premier step in preventing rereplication of DNA during a single cell division cycle...- Regulating the licensing of DNA replication origins in metazoaMelvin L DePamphilis
National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
Curr Opin Cell Biol 18:231-9. 2006.... 
Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidyZakir Ullah
National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
Cell Div 4:10. 2009..Given the fact that p21 and p57 are unique to mammals, the question arises as to whether one or both of these proteins are responsible for the induction and maintenance of polyploidy during mammalian development...- Spotlight on gemininMelvin L DePamphilis
Breast Cancer Res 13:109. 2011..Remarkably, either depletion of Geminin or over-expression of Geminin inhibits the action of TopoIIα, thereby making Geminin an excellent target for cancer chemotherapy... - The 'ORC cycle': a novel pathway for regulating eukaryotic DNA replicationMelvin L DePamphilis
National Institute of Child Health and Human Development, Building 6 416, 9000 Rockville Pike, National Institutes of Health, Bethesda, MD 20892 2753, USA
Gene 310:1-15. 2003.... - The acrosomal protein Dickkopf-like 1 (DKKL1) facilitates sperm penetration of the zona pellucidaMatthew J Kohn
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Fertil Steril 93:1533-7. 2010..To determine the role of Dkkl1 in mouse development, viability, and fertility... - The acrosomal protein Dickkopf-like 1 (DKKL1) is not essential for fertilityKotaro J Kaneko
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Fertil Steril 93:1526-32. 2010..To determine the role of Dkkl1 on mouse development, viability, and fertility... - The BAH domain facilitates the ability of human Orc1 protein to activate replication origins in vivoKohji Noguchi
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 25:5372-82. 2006..Thus, the BAH domain in human Orc1 facilitates its ability to activate replication origins in vivo by promoting association of ORC with chromatin... - Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian developmentRieko Yagi
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive, Bethesda, MD 20892 2753, USA
Development 134:3827-36. 2007..Thus, Tead4 is the earliest gene shown to be uniquely required for specification of the trophectoderm lineage... - Ubiquitylation, phosphorylation and Orc2 modulate the subcellular location of Orc1 and prevent it from inducing apoptosisTapas Saha
National Institute of Child Health and Human Development, National Institutes of Health, Building 6/3A-15, 9000 Rockville Pike, Bethesda, MD 20892-2753, USA
J Cell Sci 119:1371-82. 2006..These results provide direct evidence in support of the regulatory role proposed for Orc1, and suggest that aberrant DNA replication during mammalian development could result in apoptosis through the appearance of 'unmodified' Orc1... - Transcription factor TEAD2 is involved in neural tube closureKotaro J Kaneko
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Genesis 45:577-87. 2007..These results reveal a maternal genetic contribution to neural tube closure, and suggest that Tead2-deficient mice provide a model for anencephaly, a common human birth defect that can be prevented by folic acid... - Xenopus origin recognition complex (ORC) initiates DNA replication preferentially at sequences targeted by Schizosaccharomyces pombe ORCDaochun Kong
National Institute of Child Health and Human Development, Building 6 416, 9000 Rockville Pike, National Institutes of Health, Bethesda, MD 20892 2753, USA
EMBO J 22:3441-50. 2003..These results reveal that XlORC initiates DNA replication preferentially at the same or similar sites to those targeted in S.pombe... 
High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit themChrissie Y Lee
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, United States
Methods 57:234-48. 2012....- The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cellsJung Min Park
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 31 Center Drive, Bldg 31, Rm 2A25, Bethesda, MD 20892 2426, USA
Mol Cell Biol 26:1445-51. 2006..The results indicate that in contrast to the situation in yeasts, La is essential in mammals and is one of a limited number of genes required as early as the development of the ICM... - Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complexIlian Radichev
NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
J Biol Chem 281:23264-73. 2006..Because this suppression required only the ORC assembly and NLS domains, these domains appear to constitute the functional domain of Orc2... - An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cellsWenge Zhu
National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 2753, USA
Mol Cancer Res 9:294-310. 2011..Thus, this assay provides a new approach to the discovery of compounds useful for investigating the regulation of genome duplication and for the treatment of cancer... - Checkpoint kinase 1 prevents cell cycle exit linked to terminal cell differentiationZakir Ullah
National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
Mol Cell Biol 31:4129-43. 2011..Thus, CHK1 is part of a "G2 restriction point" that prevents premature cell cycle exit in cells programmed for terminal differentiation, a role that CHK2 cannot play... - Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatinWei-Hsin Sun
National Institute of Child Health and Human Development, Building 6/416, National Institutes of Health, Bethesda, MD 20892-2753, USA
EMBO J 21:1437-46. 2002..These results demonstrate a programmed release of XlORC from somatic cell chromatin as it enters S phase, consistent with the proposed role for ORC in preventing re-initiation of DNA replication during S phase... - Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activityZakir Ullah
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Genes Dev 22:3024-36. 2008..Expression of p57 in TG cells was restricted to G-phase nuclei to allow CDK activation of S phase. Thus, endoreduplication in TS cells is triggered by p57 inhibition of CDK1 with concomitant suppression of the DNA damage response by p21... - Assembly of the human origin recognition complex occurs through independent nuclear localization of its componentsSoma Ghosh
NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
J Biol Chem 286:23831-41. 2011..These and other results support a model in which Orc6, Orc1, and ORC(2-5) are transported independently to the nucleus where they can either assemble into ORC(1-6) or function individually... - Developmentally programmed endoreduplication in animalsZakir Ullah
Genomics of Differentiation Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Cell Cycle 8:1501-9. 2009..The critical step, however, appears to be switching from a CDK-dependent form of the anaphase promoting complex (APC) to one that functions only in the absence of CDK activity... - Methods for detecting cells in S phaseWei-Hsin Sun
National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 241:37-53. 2004 - Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycleCong jun Li
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Mol Cell Biol 22:105-16. 2002.... - DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesisMatthew J Kohn
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Mol Reprod Dev 71:516-22. 2005..These results identify a member of the Dickkopf family as a novel acrosomal protein that may be involved in acrosome assembly or function, a unique role for a secreted signaling molecule... - Site-specific ORC binding, pre-replication complex assembly and DNA synthesis at Schizosaccharomyces pombe replication originsDaochun Kong
National Institute of Child Health and Human Development, Building 6, Room 416, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
EMBO J 21:5567-76. 2002..Schizosaccharomyces pombe may be an appropriate paradigm for the complex origins found in the metazoa... - Eukaryotic DNA replication origins: reconciling disparate dataMelvin L DePamphilis
National Institute of Child Health and Human Development, Building 6, Room 416, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Cell 114:274-5. 2003 - Selective killing of cancer cells by suppression of geminin activityWenge Zhu
National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892 2753, USA
Cancer Res 69:4870-7. 2009..These results show that inhibition of geminin activity could be used to selectively kill cancer cells without harming other cells... - Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesisRobert V Intine
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 6 Center Dr, Rm 416, Bethesda, MD 20892 2753, USA
Mol Cell Biol 24:10894-904. 2004..The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis... - "The Octet": Eight Protein Kinases that Control Mammalian DNA ReplicationMelvin L DePamphilis
Program in Genomics of Differentiation, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, MD, USA
Front Physiol 3:368. 2012.... - DNA methylation may restrict but does not determine differential gene expression at the Sgy/Tead2 locus during mouse developmentKotaro J Kaneko
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Mol Cell Biol 24:1968-82. 2004.... - Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosisCong-jun Li
Growth Biology Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA
Mol Cell Biol 24:5875-86. 2004..These results reveal that the same cyclin-dependent protein kinase that initiates mitosis in mammalian cells also concomitantly inhibits assembly of functional ORC-chromatin sites...