F R DeLeo

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc An antidote for Staphylococcus aureus pneumonia?
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Exp Med 205:271-4. 2008
  2. pmc Sublytic concentrations of Staphylococcus aureus Panton-Valentine leukocidin alter human PMN gene expression and enhance bactericidal capacity
    Shawna F Graves
    Laboratory of Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Leukoc Biol 92:361-74. 2012
  3. pmc Neutrophils in innate host defense against Staphylococcus aureus infections
    Kevin M Rigby
    Laboratory of Human Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Semin Immunopathol 34:237-59. 2012
  4. doi request reprint Community-associated methicillin-resistant Staphylococcus aureus and athletes
    Natalia Malachowa
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, MT 59840, USA
    Phys Sportsmed 40:13-21. 2012
  5. pmc Mobile genetic elements of Staphylococcus aureus
    Natalia Malachowa
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT, 59840, USA
    Cell Mol Life Sci 67:3057-71. 2010
  6. pmc Host defense and pathogenesis in Staphylococcus aureus infections
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Infect Dis Clin North Am 23:17-34. 2009
  7. ncbi request reprint Modulation of phagocyte apoptosis by bacterial pathogens
    F R DeLeo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Apoptosis 9:399-413. 2004
  8. pmc Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana 59840, USA
    J Clin Invest 119:2464-74. 2009
  9. pmc Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 108:18091-6. 2011
  10. pmc Community-associated meticillin-resistant Staphylococcus aureus
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Lancet 375:1557-68. 2010

Collaborators

Detail Information

Publications80

  1. pmc An antidote for Staphylococcus aureus pneumonia?
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Exp Med 205:271-4. 2008
    ..aureus strains, protects mice against lethal pneumonia. This finding represents the first successful vaccine strategy for the treatment of staphylococcal pneumonia...
  2. pmc Sublytic concentrations of Staphylococcus aureus Panton-Valentine leukocidin alter human PMN gene expression and enhance bactericidal capacity
    Shawna F Graves
    Laboratory of Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Leukoc Biol 92:361-74. 2012
    ..We propose that PVL-mediated priming of PMNs enhances the host innate immune response...
  3. pmc Neutrophils in innate host defense against Staphylococcus aureus infections
    Kevin M Rigby
    Laboratory of Human Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Semin Immunopathol 34:237-59. 2012
    ..Here we provide an overview of the role of neutrophils in host defense against bacterial pathogens and discuss strategies employed by S. aureus to circumvent neutrophil function...
  4. doi request reprint Community-associated methicillin-resistant Staphylococcus aureus and athletes
    Natalia Malachowa
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, MT 59840, USA
    Phys Sportsmed 40:13-21. 2012
    ....
  5. pmc Mobile genetic elements of Staphylococcus aureus
    Natalia Malachowa
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT, 59840, USA
    Cell Mol Life Sci 67:3057-71. 2010
    ..Our understanding of S. aureus MGEs and the molecules they encode has played an important role toward these ends and has provided detailed insight into the evolution of antimicrobial resistance mechanisms and virulence...
  6. pmc Host defense and pathogenesis in Staphylococcus aureus infections
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Infect Dis Clin North Am 23:17-34. 2009
    ..This article reviews human neutrophil function in the context of S aureus virulence mechanisms and provides an overview of community-associated methicillin-resistant S aureus pathogenicity...
  7. ncbi request reprint Modulation of phagocyte apoptosis by bacterial pathogens
    F R DeLeo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Apoptosis 9:399-413. 2004
    ..This review summarizes what is currently known about modulation of phagocyte apoptosis by bacteria and describes a paradigm whereby bacteria-induced neutrophil apoptosis plays a role in the resolution of infection...
  8. pmc Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana 59840, USA
    J Clin Invest 119:2464-74. 2009
    ..This Review focuses on progress made toward understanding the success of community-associated MRSA as a human pathogen, with an emphasis on genome-wide approaches and virulence determinants...
  9. pmc Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 108:18091-6. 2011
    ..aureus, whereas key SNPs in contemporary CC30 clones restrict these pathogens to hospital settings in which the host is typically compromised...
  10. pmc Community-associated meticillin-resistant Staphylococcus aureus
    Frank R Deleo
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Lancet 375:1557-68. 2010
    ..Here we review the most up-to-date knowledge and provide a perspective for the future prophylaxis or new treatments for CA-MRSA infections...
  11. ncbi request reprint Is Panton-Valentine leukocidin the major virulence determinant in community-associated methicillin-resistant Staphylococcus aureus disease?
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Infect Dis 194:1761-70. 2006
    ..Although the toxin may be a highly linked epidemiological marker for CA-MRSA strains, we conclude that PVL is not the major virulence determinant of CA-MRSA...
  12. ncbi request reprint Neutrophil microbicides induce a pathogen survival response in community-associated methicillin-resistant Staphylococcus aureus
    Amy M Palazzolo-Ballance
    Laboratory of Human Bacterial Pathogenesis, Research Technologies Section, Genomics Unit, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Immunol 180:500-9. 2008
    ..Collectively, the data provide an enhanced view of the mechanisms used by S. aureus to circumvent destruction by the innate immune system...
  13. pmc A point mutation in the agr locus rather than expression of the Panton-Valentine leukocidin caused previously reported phenotypes in Staphylococcus aureus pneumonia and gene regulation
    Amer E Villaruz
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 200:724-34. 2009
    ..Finally, our work emphasizes the need to frequently evaluate the integrity of the S. aureus agr locus...
  14. doi request reprint An update on community-associated MRSA virulence
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Curr Opin Pharmacol 9:545-51. 2009
    ..This review focuses on current progress toward understanding the enhanced virulence properties of CA-MRSA, with an emphasis on Panton-Valentine leukocidin, alpha-hemolysin, and the recently discovered alpha-type phenol-soluble modulins...
  15. pmc Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection
    Christopher Burlak
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Cell Microbiol 9:1172-90. 2007
    ..We conclude that the exoproteins identified herein likely account in part for the success of CA-MRSA as a human pathogen...
  16. pmc Epidemic community-associated methicillin-resistant Staphylococcus aureus: recent clonal expansion and diversification
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 105:1327-32. 2008
    ..aureus (known as phage-type 80/81 S. aureus) in the 1950s...
  17. pmc In vitro serial passage of Staphylococcus aureus: changes in physiology, virulence factor production, and agr nucleotide sequence
    Greg A Somerville
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases NIH, 903 South 4th Street, Hamilton, Montana 59840, USA
    J Bacteriol 184:1430-7. 2002
    ..aureus undergoes significant phenotypic and genotypic changes during serial passage and suggest that vigilance should be used when extrapolating data obtained from the study of high-passage strains...
  18. pmc Genome-wide molecular dissection of serotype M3 group A Streptococcus strains causing two epidemics of invasive infections
    Stephen B Beres
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:11833-8. 2004
    ..Genome-wide analysis of population-based strain samples cultured from clinically well defined patients is crucial for understanding the molecular events underlying bacterial epidemics...
  19. ncbi request reprint FcepsilonRI- and Fcgamma receptor-mediated production of reactive oxygen species by mast cells is lipoxygenase- and cyclooxygenase-dependent and NADPH oxidase-independent
    Emily J Swindle
    Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1881, USA
    J Immunol 179:7059-71. 2007
    ....
  20. ncbi request reprint STAT3 mutations in the hyper-IgE syndrome
    Steven M Holland
    National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    N Engl J Med 357:1608-19. 2007
    ..Inheritance is autosomal dominant; sporadic cases are also found...
  21. ncbi request reprint Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
    J Immunol 175:3907-19. 2005
    ..Our studies reveal a gene transcription program in a prominent human pathogen that likely contributes to evasion of innate host defense...
  22. ncbi request reprint Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA
    Rong Wang
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, 903 South 4th Street, Hamilton, Montana 59840, USA
    Nat Med 13:1510-4. 2007
    ..Our study reveals a previously uncharacterized set of S. aureus virulence factors that account at least in part for the enhanced virulence of CA-MRSA...
  23. ncbi request reprint Engagement of the pathogen survival response used by group A Streptococcus to avert destruction by innate host defense
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Immunol 173:1194-201. 2004
    ..Importantly, our studies identify specific genes in the pathogen survival response as potential targets to control human infections...
  24. ncbi request reprint Relapsing fever spirochaetes produce a serine protease that provides resistance to oxidative stress and killing by neutrophils
    Cyril Guyard
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Mol Microbiol 60:710-22. 2006
    ..This periplasmic enzyme may prevent the accumulation of proteins damaged by the innate immune response and contribute to the ability of the relapsing fever spirochaetes to achieve high cell densities in blood...
  25. pmc Characterization of an extracellular virulence factor made by group A Streptococcus with homology to the Listeria monocytogenes internalin family of proteins
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:7043-52. 2003
    ..These studies characterize the first GAS LRR protein as an extracellular virulence factor that contributes to pathogenesis and may participate in evasion of the innate host defense...
  26. pmc Host-microbe interaction systems biology: lifecycle transcriptomics and comparative genomics
    Daniel E Sturdevant
    Genomics Unit, Research Technologies Section, Research Technologies Branch, Rocky Mountain Laboratories, NIH, 904 South 4th Street, Hamilton, MT 59840, USA
    Future Microbiol 5:205-19. 2010
    ..Together, microarray and comparative genomic technologies will continue to advance our understanding of pathogen evolution and assist in combating human infectious disease...
  27. pmc Relative contribution of Panton-Valentine leukocidin to PMN plasma membrane permeability and lysis caused by USA300 and USA400 culture supernatants
    Shawna F Graves
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Microbes Infect 12:446-56. 2010
    ..aureus culture supernatants have limited ability to estimate the relative contribution of PVL to pathogenesis (or cytolysis in vitro or in vivo), especially when assayed using culture media that promote selective high production of PVL...
  28. ncbi request reprint Evasion of human innate and acquired immunity by a bacterial homolog of CD11b that inhibits opsonophagocytosis
    B Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA
    Nat Med 7:1298-305. 2001
    ....
  29. pmc Community-associated methicillin-resistant Staphylococcus aureus immune evasion and virulence
    Shawna F Graves
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA
    J Mol Med (Berl) 88:109-14. 2010
    ..In this review, we discuss the role of neutrophils in host defense against S. aureus and highlight progress made toward understanding mechanisms of CA-MRSA virulence and pathogenesis...
  30. ncbi request reprint Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain
    David J Banks
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    J Infect Dis 190:727-38. 2004
    ..Our studies add to the theme that GAS prophage-encoded extracellular proteins contribute to host-pathogen interactions in a strain-specific fashion...
  31. pmc Identification of a novel Staphylococcus aureus two-component leukotoxin using cell surface proteomics
    Christy L Ventura
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America
    PLoS ONE 5:e11634. 2010
    ..We conclude LukGH is a novel two-component leukotoxin with cytolytic activity toward neutrophils, and thus potentially contributes to S. aureus virulence...
  32. pmc Genome-wide protective response used by group A Streptococcus to evade destruction by human polymorphonuclear leukocytes
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 100:1996-2001. 2003
    ..The gene regulatory program revealed by our studies identifies previously undescribed potential vaccine antigens and targets for therapeutic interventions designed to control GAS infections...
  33. pmc Opsonophagocytosis-inhibiting mac protein of group a streptococcus: identification and characteristics of two genetic complexes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:6880-90. 2002
    ..Taken together, the data add to the emerging theme in GAS pathogenesis that allelic variation in virulence genes contributes to fundamental differences in host-pathogen interactions among strains...
  34. ncbi request reprint An apoptosis differentiation programme in human polymorphonuclear leucocytes
    S D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Biochem Soc Trans 32:474-6. 2004
    ..Collectively, these studies establish a global model of host cell-pathogen interaction, which provides fundamental insight into the resolution of infection in humans...
  35. ncbi request reprint A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence
    Cuong Vuong
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, NIAID, National Institutes of Health, Hamilton, Montana 59840, USA
    J Biol Chem 279:54881-6. 2004
    ..Notably, this general virulence mechanism is likely similar for other pathogenic bacteria and constitutes an excellent target for therapeutic maneuvers aimed at combating biofilm-associated infection...
  36. ncbi request reprint Gene expression profiling provides insight into the pathophysiology of chronic granulomatous disease
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    J Immunol 172:636-43. 2004
    ....
  37. ncbi request reprint Host-pathogen interactions: leukocyte phagocytosis and associated sequelae
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Methods Cell Sci 24:79-90. 2002
    ..Taken together, these assays provide rapid and accurate assessment of critical PMN processes...
  38. doi request reprint Neutrophil apoptosis and the resolution of infection
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Immunol Res 43:25-61. 2009
    ..However, some bacterial pathogens alter PMN apoptosis to survive and thereby cause disease. Herein, we review PMN apoptosis and the ability of microorganisms to alter this important process...
  39. pmc Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA
    Shu Y Queck
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA
    PLoS Pathog 5:e1000533. 2009
    ..Thus, our study reveals a previously unknown role of methicillin resistance clusters in staphylococcal pathogenesis and shows that important virulence and antibiotic resistance determinants may be combined in staphylococcal MGEs...
  40. pmc Evolution of virulence in epidemic community-associated methicillin-resistant Staphylococcus aureus
    Min Li
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, MD 20892, USA
    Proc Natl Acad Sci U S A 106:5883-8. 2009
    ..This finding calls for a profound revision of our notion about CA-MRSA pathogenesis at the molecular level and has important implications for design of therapeutics directed against CA-MRSA...
  41. ncbi request reprint Down-regulation of proinflammatory capacity during apoptosis in human polymorphonuclear leukocytes
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Immunol 170:3357-68. 2003
    ..These findings provide new insight into the molecular events that resolve inflammation following PMN activation in humans...
  42. ncbi request reprint Transmission of Yersinia pestis from an infectious biofilm in the flea vector
    Clayton O Jarrett
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Infect Dis 190:783-92. 2004
    ..pestis. Enabling arthropod-borne transmission represents a novel function of a bacterial biofilm...
  43. ncbi request reprint An apoptosis-differentiation program in human polymorphonuclear leukocytes facilitates resolution of inflammation
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    J Leukoc Biol 73:315-22. 2003
    ..These findings provide new insight into the molecular events that contribute to the resolution of inflammation in humans...
  44. pmc Targeting of alpha-hemolysin by active or passive immunization decreases severity of USA300 skin infection in a mouse model
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Infect Dis 202:1050-8. 2010
    ..We conclude that Hla is a potential target for therapeutics or vaccines designed to moderate severe S. aureus skin infections...
  45. pmc Postgenomic analysis of four novel antigens of group a streptococcus: growth phase-dependent gene transcription and human serologic response
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 184:6316-24. 2002
    ..These results are additional evidence that postgenomic strategies provide new ways to identify and investigate novel bacterial proteins that may participate in host-pathogen interactions or serve as targets for therapeutics research...
  46. pmc Global changes in gene expression by human polymorphonuclear leukocytes during receptor-mediated phagocytosis: cell fate is regulated at the level of gene expression
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 99:6901-6. 2002
    ..Most significantly, we discovered that programmed cell death is regulated at the level of gene expression. Thus, we hypothesize that gene regulation in PMNs facilitates resolution of inflammatory responses...
  47. ncbi request reprint Dectin-1 promotes fungicidal activity of human neutrophils
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Eur J Immunol 37:467-78. 2007
    ..8% for beta-glucan and 36.2% for GE2, p<0.01). Our results reveal a mechanism whereby PMN dectin-1 plays a key role in the recognition and killing of fungal pathogens by the innate immune system...
  48. ncbi request reprint Spontaneous neutrophil apoptosis and regulation of cell survival by granulocyte macrophage-colony stimulating factor
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    J Leukoc Biol 78:1408-18. 2005
    ..These studies provide a global view of the molecular events that regulate neutrophil survival and apoptosis...
  49. pmc Complete nucleotide sequence analysis of plasmids in strains of Staphylococcus aureus clone USA300 reveals a high level of identity among isolates with closely related core genome sequences
    Adam D Kennedy
    Laboratory of Human Bacterial Pathogenesis, Genomics Unit, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    J Clin Microbiol 48:4504-11. 2010
    ..The high level of plasmid sequence identity among the majority of closely related USA300 isolates is consistent with the recent clonal emergence hypothesis for USA300...
  50. pmc Key role of poly-gamma-DL-glutamic acid in immune evasion and virulence of Staphylococcus epidermidis
    Stanislava Kocianova
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases NIAID, NIH, Hamilton, Montana 59840, USA
    J Clin Invest 115:688-94. 2005
    ..Our study presents important novel biological functions for PGA and indicates that PGA represents an excellent target for therapeutic maneuvers aimed at treating disease caused by S. epidermidis and related staphylococci...
  51. ncbi request reprint Regulation of the neutrophil-mediated inflammatory response to infection
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th Street, Hamilton, MT 59840, USA
    Microbes Infect 5:1337-44. 2003
    ....
  52. pmc Identification and characterization of HtsA, a second heme-binding protein made by Streptococcus pyogenes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Infect Immun 71:5962-9. 2003
    ..Our results suggest that HtsABC is an ABC transporter that may participate in heme acquisition in GAS...
  53. pmc Bacterial pathogens modulate an apoptosis differentiation program in human neutrophils
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 100:10948-53. 2003
    ..pyogenes alters the apoptosis differentiation program in neutrophils, resulting in pathogen survival and disease...
  54. ncbi request reprint Apoptosis in human polymorphonuclear leukocytes: searching for a genetic roadmap
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Arch Immunol Ther Exp (Warsz) 51:1-8. 2003
    ..Further characterization of the apoptosis-differentiation program and associated biochemical pathways in mature PMNs will likely yield important insights into the resolution of inflammation and infection...
  55. ncbi request reprint Maturation of human neutrophil phagosomes includes incorporation of molecular chaperones and endoplasmic reticulum quality control machinery
    Christopher Burlak
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Mol Cell Proteomics 5:620-34. 2006
    ..We conclude that neutrophil phagosomes have heretofore unrecognized complexity and function, which includes potential for antigen processing events...
  56. ncbi request reprint Neutrophils in the innate immune response
    Scott D Kobayashi
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Arch Immunol Ther Exp (Warsz) 53:505-17. 2005
    ..This review provides a general overview of the role of human neutrophils in the innate host response to infection and summarizes some of the recent advances in neutrophil biology...
  57. pmc Autosomal dominant and sporadic monocytopenia with susceptibility to mycobacteria, fungi, papillomaviruses, and myelodysplasia
    Donald C Vinh
    Immunopathogenesis Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 115:1519-29. 2010
    ..This novel clinical syndrome links susceptibility to mycobacterial, viral, and fungal infections with malignancy and can be transmitted in an autosomal dominant pattern...
  58. pmc Insight into the molecular basis of pathogen abundance: group A Streptococcus inhibitor of complement inhibits bacterial adherence and internalization into human cells
    Nancy P Hoe
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 99:7646-51. 2002
    ..Sic enhances bacterial survival by enabling the pathogen to avoid the intracellular environment. This process contributes to the abundance of M1 GAS in human infections and their ability to cause epidemics...
  59. ncbi request reprint Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system
    Cuong Vuong
    Laboratories of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institute of Health, Hamilton, MT 59840, USA
    Cell Microbiol 6:269-75. 2004
    ..PIA represents the first defined factor of the staphylococcal biofilm matrix that protects against major components of human innate host defence...
  60. ncbi request reprint Streptococcus pyogenes and human neutrophils: a paradigm for evasion of innate host defense by bacterial pathogens
    Jovanka M Voyich
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th Street, Hamilton, MT 59840, USA
    Microbes Infect 6:1117-23. 2004
    ..This review focuses on the interaction of human PMNs with Streptococcus pyogenes as a paradigm for successful pathogen evasion mechanisms...
  61. doi request reprint The arginine catabolic mobile element and staphylococcal chromosomal cassette mec linkage: convergence of virulence and resistance in the USA300 clone of methicillin-resistant Staphylococcus aureus
    Binh An Diep
    Division of Infectious Diseases, Department of Medicine, San Francisco General Hospital, University of California, San Francisco 94110, USA
    J Infect Dis 197:1523-30. 2008
    ..These data are consistent with a model in which ACME enhances growth and survival of USA300, allowing for genetic "hitchhiking" of SCCmec. SCCmec in turn protects against exposure to beta-lactams...
  62. ncbi request reprint Poring over pores: alpha-hemolysin and Panton-Valentine leukocidin in Staphylococcus aureus pneumonia
    Juliane Bubeck Wardenburg
    Nat Med 13:1405-6. 2007
  63. pmc Central role of a bacterial two-component gene regulatory system of previously unknown function in pathogen persistence in human saliva
    Samuel A Shelburne
    Department of Medicine, Center for Human Bacterial Pathogenesis, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 102:16037-42. 2005
    ..The discovery of a genetic program that significantly increased persistence of a major human pathogen in saliva enhances understanding of how bacteria survive in the host and suggests new therapeutic strategies...
  64. ncbi request reprint Insights into pathogen immune evasion mechanisms: Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils
    Dori L Borjesson
    Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    J Immunol 174:6364-72. 2005
    ..phagocytophilum fails to trigger the apoptosis differentiation program usually induced by bacteria, and 2) a protein or molecule on the pathogen surface can mediate an early delay in spontaneous neutrophil apoptosis...
  65. pmc A chemokine-degrading extracellular protease made by group A Streptococcus alters pathogenesis by enhancing evasion of the innate immune response
    Paul Sumby
    Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, 6565 Fannin Street, Houston, TX 77030, USA
    Infect Immun 76:978-85. 2008
    ..The data indicate that SpyCEP contributes to GAS virulence in a strain- and disease-dependent manner...
  66. ncbi request reprint Analysis of Staphylococcus aureus gene expression during PMN phagocytosis
    Jovanka M Voyich
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA
    Methods Mol Biol 431:109-22. 2008
    ..aureus pathogenesis. This chapter describes methods to analyze gene expression in S. aureus during interaction with human neutrophils...
  67. doi request reprint Community-associated methicillin-resistant Staphylococcus aureus skin infections: advances toward identifying the key virulence factors
    Tyler K Nygaard
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59718, USA
    Curr Opin Infect Dis 21:147-52. 2008
    ..The purpose of this review is to provide a critical analysis of our current knowledge of virulence factors contributing to skin and soft-tissue infections caused by CA-MRSA...
  68. pmc Differential distribution and expression of Panton-Valentine leucocidin among community-acquired methicillin-resistant Staphylococcus aureus strains
    Battouli Said-Salim
    TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ, 07103, USA
    J Clin Microbiol 43:3373-9. 2005
    ..Unexpectedly, there was no correlation between PVL expression and PMN lysis, suggesting that additional virulence factors underlie leukotoxicity and, thus, the pathogenesis of CA-MRSA...
  69. pmc Panton-Valentine leukocidin is not a virulence determinant in murine models of community-associated methicillin-resistant Staphylococcus aureus disease
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    J Infect Dis 198:1166-70. 2008
    ..The data presented in this report support the observation that PVL does not contribute to the pathogenesis of staphylococcal infection of mice...
  70. ncbi request reprint The expanding role of NADPH oxidases in health and disease: no longer just agents of death and destruction
    Mark T Quinn
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA
    Clin Sci (Lond) 111:1-20. 2006
    ..Herein, we summarize our current understanding of NADPH oxidases and provide an overview of how they contribute to specific human diseases...
  71. ncbi request reprint Genome-scale transcript analyses in human neutrophils
    Scott D Kobayashi
    Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA
    Methods Mol Biol 412:441-53. 2007
    ..Notably, the procedures utilize commercially available reagents and materials and thus represent a standardized approach for evaluating PMN transcript levels...
  72. pmc Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus
    Stephen B Beres
    Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:7059-64. 2006
    ..Our analysis provided unprecedented detail about the molecular anatomy of bacterial strain genotype-patient phenotype relationships...
  73. ncbi request reprint ADAM17 activity during human neutrophil activation and apoptosis
    Bruce Walcheck
    Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA
    Eur J Immunol 36:968-76. 2006
    ..Our data thus indicate prolonged ADAM17 expression during neutrophil effector functions. The implications of this may be a role by ADAM17 in both the induction and down-regulation of neutrophil activity...
  74. ncbi request reprint Neutrophil methods and protocols. Preface
    Mark T Quinn
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, USA
    Methods Mol Biol 412:vii-viii. 2007
  75. pmc Toward a genome-wide systems biology analysis of host-pathogen interactions in group A Streptococcus
    James M Musser
    Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, and Department of Pathology, The Methodist Hospital, Houston, TX 77030, USA
    Am J Pathol 167:1461-72. 2005
    ..In addition, we hope the review will stimulate investigators to consider using analogous approaches for analysis of the molecular pathogenesis of other microbes...
  76. ncbi request reprint Role of NF-kappaB in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-alpha
    Katherine A Gauss
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA
    J Leukoc Biol 82:729-41. 2007
    ....
  77. ncbi request reprint Variants of the 5'-untranslated region of human NCF2: expression and translational efficiency
    Katherine A Gauss
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA
    Gene 366:169-79. 2006
    ..These studies suggest that expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation...
  78. ncbi request reprint Methods molecular biology. Preface
    Michael Otto
    Methods Mol Biol 431:V-VI. 2008
  79. ncbi request reprint A plague upon the phagocytes
    Frank R Deleo
    Nat Med 11:927-8. 2005
  80. pmc Maltodextrin utilization plays a key role in the ability of group A Streptococcus to colonize the oropharynx
    Samuel A Shelburne
    Section of Infectious Diseases, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA
    Infect Immun 74:4605-14. 2006
    ..Further investigation into carbohydrate transport and metabolism pathways may yield novel insights into GAS pathogenesis...