John A Cidlowski

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Glucocorticoids and their actions in cells
    John A Cidlowski
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Retina 29:S21-3. 2009
  2. pmc Reciprocal epigenetic modification of histone H2B occurs in chromatin during apoptosis in vitro and in vivo
    K Ajiro
    Laboratory of Signal Transduction, National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell Death Differ 17:984-93. 2010
  3. pmc A role for glucocorticoids in stress-impaired reproduction: beyond the hypothalamus and pituitary
    Shannon Whirledge
    NIEHS NIH, MD F3 07, P O Box 12233, Research Triangle Park, North Carolina 27709
    Endocrinology 154:4450-68. 2013
  4. pmc Ligand-independent phosphorylation of the glucocorticoid receptor integrates cellular stress pathways with nuclear receptor signaling
    Amy Jo Galliher-Beckley
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 31:4663-75. 2011
  5. ncbi request reprint Functional analysis of the LXXLL motifs of the human glucocorticoid receptor: association with altered ligand affinity
    Diane D Dong
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, United States
    J Steroid Biochem Mol Biol 101:106-17. 2006
  6. ncbi request reprint Ligand-selective targeting of the glucocorticoid receptor to nuclear subdomains is associated with decreased receptor mobility
    Marcel J M Schaaf
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Mol Endocrinol 19:1501-15. 2005
  7. pmc Selective regulation of bone cell apoptosis by translational isoforms of the glucocorticoid receptor
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 27:7143-60. 2007
  8. ncbi request reprint Translational regulatory mechanisms generate N-terminal glucocorticoid receptor isoforms with unique transcriptional target genes
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Mol Cell 18:331-42. 2005
  9. pmc Human glucocorticoid receptor beta binds RU-486 and is transcriptionally active
    Laura J Lewis-Tuffin
    Laboratory of Signal Transduciton, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 27:2266-82. 2007
  10. pmc Molecular evidence for a link between the N363S glucocorticoid receptor polymorphism and altered gene expression
    Christine M Jewell
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F3 07, Research Triangle Park, North Carolina 27709, USA
    J Clin Endocrinol Metab 92:3268-77. 2007

Collaborators

Detail Information

Publications75

  1. ncbi request reprint Glucocorticoids and their actions in cells
    John A Cidlowski
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Retina 29:S21-3. 2009
    ..Recent progress predicts an increasing precision with which GC steroids can be used to influence biologic functions...
  2. pmc Reciprocal epigenetic modification of histone H2B occurs in chromatin during apoptosis in vitro and in vivo
    K Ajiro
    Laboratory of Signal Transduction, National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell Death Differ 17:984-93. 2010
    ..These findings suggest that selective chromatin post-translational modifications are associated with DNA degradation during apoptosis...
  3. pmc A role for glucocorticoids in stress-impaired reproduction: beyond the hypothalamus and pituitary
    Shannon Whirledge
    NIEHS NIH, MD F3 07, P O Box 12233, Research Triangle Park, North Carolina 27709
    Endocrinology 154:4450-68. 2013
    ....
  4. pmc Ligand-independent phosphorylation of the glucocorticoid receptor integrates cellular stress pathways with nuclear receptor signaling
    Amy Jo Galliher-Beckley
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 31:4663-75. 2011
    ..This posttranslational modification allows prior cellular stress signals to dictate the transcriptional response to glucocorticoids...
  5. ncbi request reprint Functional analysis of the LXXLL motifs of the human glucocorticoid receptor: association with altered ligand affinity
    Diane D Dong
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, United States
    J Steroid Biochem Mol Biol 101:106-17. 2006
    ..These data suggest that although LXXLL motifs in helices 1 and 10 appear to lie outside the predicted ligand binding pocket they may contribute to receptor ligand binding affinity...
  6. ncbi request reprint Ligand-selective targeting of the glucocorticoid receptor to nuclear subdomains is associated with decreased receptor mobility
    Marcel J M Schaaf
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Mol Endocrinol 19:1501-15. 2005
    ..Thus, glucocorticoid receptors can be targeted (depending on the ligand) to relatively immobile nuclear subdomains. The transient association of receptor with these domains decreases the mobility of the receptor...
  7. pmc Selective regulation of bone cell apoptosis by translational isoforms of the glucocorticoid receptor
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 27:7143-60. 2007
    ..Together, these findings provide new evidence that translational glucocorticoid receptor isoforms can elicit distinct glucocorticoid responses and may be useful for the development of safe glucocorticoids with reduced side effects...
  8. ncbi request reprint Translational regulatory mechanisms generate N-terminal glucocorticoid receptor isoforms with unique transcriptional target genes
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Mol Cell 18:331-42. 2005
    ..Based on these observations, we propose that cell-type specific GR isoforms generate specificity in glucocorticoid control of transcription in different tissues...
  9. pmc Human glucocorticoid receptor beta binds RU-486 and is transcriptionally active
    Laura J Lewis-Tuffin
    Laboratory of Signal Transduciton, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 27:2266-82. 2007
    ....
  10. pmc Molecular evidence for a link between the N363S glucocorticoid receptor polymorphism and altered gene expression
    Christine M Jewell
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F3 07, Research Triangle Park, North Carolina 27709, USA
    J Clin Endocrinol Metab 92:3268-77. 2007
    ..However, molecular studies in vitro using reporter gene expression systems have failed, for the most part, to define a link between this polymorphism and altered glucocorticoid receptor function...
  11. pmc Molecular origins for the dominant negative function of human glucocorticoid receptor beta
    Matthew R Yudt
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Mol Cell Biol 23:4319-30. 2003
    ..Molecular modeling of wild-type and mutant hGRalpha and hGRbeta provides structural insight and a potential physical explanation for the lack of hormone binding and the dominant negative actions of hGRbeta...
  12. ncbi request reprint Development of a flow cytometric assay to study glucocorticoid receptor-mediated gene activation in living cells
    Brian M Necela
    Department of Health and Human Services, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Building 101, MD F3 07, Research Triangle Park, NC 27709, USA
    Steroids 68:341-50. 2003
    ..Taken together, these data suggest the reporter-based flow cytometry assay is an effective method for analyzing glucocorticoid receptor-mediated gene expression at the single cell level in living cells...
  13. ncbi request reprint Activation of intrinsic and extrinsic pathways in apoptotic signaling during UV-C-induced death of Jurkat cells: the role of caspase inhibition
    Alyson B Scoltock
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, NC 27709, USA
    Exp Cell Res 297:212-23. 2004
    ..Thus, both intrinsic and extrinsic pathways are activated during UV-induced apoptosis in Jurkat cells and this redundancy appears to assure cell death during selective caspase inhibition...
  14. pmc Complex human glucocorticoid receptor dim mutations define glucocorticoid induced apoptotic resistance in bone cells
    Christine M Jewell
    National Institute of Environmental Health Sciences National Institutes of Health, Laboratory of Signal Transduction, Research Triangle Park, North Carolina 27709, USA
    Mol Endocrinol 26:244-56. 2012
    ..These studies challenge conclusions drawn from previous studies of GR dim mutants...
  15. pmc Glucocorticoid receptor alpha isoform-selective regulation of antiapoptotic genes in osteosarcoma cells: a new mechanism for glucocorticoid resistance
    Katherine L Gross
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Mol Endocrinol 25:1087-99. 2011
    ..These data suggest that the N-terminal translational isoforms of GRα selectively regulate antiapoptotic genes and that the GRα-D isoform may contribute to the resistance of certain cancer cells to glucocorticoid-induced apoptosis...
  16. pmc An endogenous calcium-dependent, caspase-independent intranuclear degradation pathway in thymocyte nuclei: antagonism by physiological concentrations of K(+) ions
    Kozo Ajiro
    Laboratory of Signal Transduction, National Institute of Environmental Health Science, National Institute of Health, Research Triangle Park, NC 27709, USA
    Exp Cell Res 314:1237-49. 2008
    ..Together, these data define an inherent caspase-independent catabolic pathway in thymocyte nuclei that is sensitive to physiological concentrations of intracellular cations...
  17. pmc DAX-1 (dosage-sensitive sex reversal-adrenal hypoplasia congenita critical region on the X-chromosome, gene 1) selectively inhibits transactivation but not transrepression mediated by the glucocorticoid receptor in a LXXLL-dependent manner
    Junguo Zhou
    Laboratory of Signal Transduction, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Mol Endocrinol 22:1521-34. 2008
    ..Thus we demonstrate that DAX-1 directly modulates GR signaling in addition to affecting glucocorticoid hormone levels...
  18. pmc Glutathione depletion and disruption of intracellular ionic homeostasis regulate lymphoid cell apoptosis
    Rodrigo Franco
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 283:36071-87. 2008
    ..These results suggest that deregulation of GSH and ionic homeostasis converge in the regulation of apoptosis in lymphoid cells...
  19. ncbi request reprint Uncoupling cell shrinkage from apoptosis reveals that Na+ influx is required for volume loss during programmed cell death
    Carl D Bortner
    Laboratory of Signal Transduction, NIEHS, Department of Health and Human Services, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:39176-84. 2003
    ..Therefore cell shrinkage can be separated from other features of apoptosis...
  20. ncbi request reprint Glucocorticoid receptor isoforms generate transcription specificity
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH DHHS, Research Triangle Park, NC 27709, USA
    Trends Cell Biol 16:301-7. 2006
    ..Here, we discuss a new molecular model potentially underlying tissue-specific glucocorticoid resistance and selectivity...
  21. pmc Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis
    Carl D Bortner
    Laboratory of Signal Transduction, National Institutes of Health Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 283:7219-29. 2008
    ..Together these studies demonstrate that AVD occurs in two distinct stages with the earliest stage reflecting a cellular cationic gradient reversal...
  22. ncbi request reprint Selective role of intracellular chloride in the regulation of the intrinsic but not extrinsic pathway of apoptosis in Jurkat T-cells
    Gerd Heimlich
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Research Triangle Park, NC 27709, USA
    J Biol Chem 281:2232-41. 2006
    ..Thus, an alteration of intracellular chloride plays an important role in the activation of signaling molecules upstream of the mitochondria, specifically impairing the intrinsic but not extrinsic apoptotic pathway...
  23. ncbi request reprint The human glucocorticoid receptor: one gene, multiple proteins and diverse responses
    Junguo Zhou
    Molecular Endocrinology Group, The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIEHS NIH, DHHS, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Steroids 70:407-17. 2005
    ..We speculate that the composition and proportion of individual isoforms expressed in particular cellular contexts account for the diverse effects of glucocorticoid hormones...
  24. pmc Ouabain-induced perturbations in intracellular ionic homeostasis regulate death receptor-mediated apoptosis
    Mihalis I Panayiotidis
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIEHS, National Institutes of Health, Research Triangle Park, NC, 27709, USA
    Apoptosis 15:834-49. 2010
    ..Our data suggest that the impairment of the Na(+)-K(+)-ATPase activity during apoptosis is linked to perturbations in cell Ca(2+) homeostasis that modulate apoptosis induced by the activation of Fas by FasL...
  25. ncbi request reprint Stimulation of Kv1.3 potassium channels by death receptors during apoptosis in Jurkat T lymphocytes
    Nina M Storey
    Membrane Signaling Group, Laboratory of Signal Transduction, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:33319-26. 2003
    ..Thus, Fas ligand increases Kv1.3 channel activity through the same canonical apoptotic signaling cascade that is required for potassium efflux, cell shrinkage, and apoptosis...
  26. ncbi request reprint New approaches for determining apoptotic volume decrease in cells
    Carl D Bortner
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health, Research Triangle Park, North Carolina, USA
    Methods Enzymol 428:161-81. 2007
    ..This chapter describes several methods for using the Cell Lab Quanta SC to study AVD during apoptosis...
  27. ncbi request reprint Molecular mechanisms of glucocorticoid action and resistance
    Marcel J M Schaaf
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, P O Box 12233, Research Triangle Park, NC 27709, USA
    J Steroid Biochem Mol Biol 83:37-48. 2002
    ..It has been shown that these mechanisms can significantly inhibit glucocorticoid signaling, and could therefore seriously decrease the efficacy of glucocorticoids used clinically...
  28. doi request reprint Sexually dimorphic actions of glucocorticoids provide a link to inflammatory diseases with gender differences in prevalence
    Danielle Duma
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, MD F3 07, Research Triangle Park, NC 27709, USA
    Sci Signal 3:ra74. 2010
    ..These gender-specific actions of glucocorticoids in liver were substantiated in vivo with a sepsis model of systemic inflammation...
  29. pmc Deep sequencing identification of novel glucocorticoid-responsive miRNAs in apoptotic primary lymphocytes
    Lindsay K Smith
    Molecular Endocrinology Group, Laboratory of Signal Transduction, NIEHS, NIH, Department of Health and Human Services, Research Triangle Park, North Carolina, United States of America
    PLoS ONE 8:e78316. 2013
    ....
  30. ncbi request reprint Global gene expression analysis in human uterine epithelial cells defines new targets of glucocorticoid and estradiol antagonism
    Shannon Whirledge
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina
    Biol Reprod 89:66. 2013
    ....
  31. pmc Ligand-induced repression of the glucocorticoid receptor gene is mediated by an NCoR1 repression complex formed by long-range chromatin interactions with intragenic glucocorticoid response elements
    Sivapriya Ramamoorthy
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, USA
    Mol Cell Biol 33:1711-22. 2013
    ..Consequently, the chronic nature of inflammatory conditions involving long-term glucocorticoid administration may lead to constitutive repression of GR gene transcription and thus to glucocorticoid resistance...
  32. pmc Estradiol antagonism of glucocorticoid-induced GILZ expression in human uterine epithelial cells and murine uterus
    Shannon Whirledge
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Endocrinology 154:499-510. 2013
    ..Regulation of the antiinflammatory gene GILZ by glucocorticoids and E(2) suggests cross talk between the immune modulating functions of glucocorticoids and the reproductive actions of estradiol signaling...
  33. pmc Proinflammatory actions of glucocorticoids: glucocorticoids and TNFα coregulate gene expression in vitro and in vivo
    Erica A Lannan
    Laboratory of Signal Transduction, National Institutes of Environmental Health Sciences, 111 T W Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    Endocrinology 153:3701-12. 2012
    ..This discovery may underlie the basis of some of the adverse effects associated with long-term glucocorticoid therapy...
  34. pmc Osmotic stress resistance imparts acquired anti-apoptotic mechanisms in lymphocytes
    Carl D Bortner
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 287:6284-95. 2012
    ..These results define a critical role of volume regulation mechanisms in apoptotic resistance...
  35. pmc Glucocorticoids regulate tristetraprolin synthesis and posttranscriptionally regulate tumor necrosis factor alpha inflammatory signaling
    Kathleen Smoak
    Department of Health and Human Services, Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health, Building 101, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 26:9126-35. 2006
    ..These studies provide the molecular evidence for glucocorticoid regulation of human TTP and reflect a novel inductive anti-inflammatory signaling pathway for glucocorticoids that acts via posttranscriptional mechanisms...
  36. pmc Glucocorticoids modulate microRNA expression and processing during lymphocyte apoptosis
    Lindsay K Smith
    Molecular Endocrinology Group, Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 285:36698-708. 2010
    ....
  37. ncbi request reprint Multiple glucocorticoid receptor isoforms and mechanisms of post-translational modification
    Danielle Duma
    Laboratory of Signal Transduction, Molecular Endocrinology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Steroid Biochem Mol Biol 102:11-21. 2006
    ..We outline the recent advances made in identifying the processes that generate and modify multiple GR isoforms and the post-translational modifications that contribute to the increasing diversity in the glucocorticoid signaling pathway...
  38. pmc Dual role for glucocorticoids in cardiomyocyte hypertrophy and apoptosis
    Rongqin Ren
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Endocrinology 153:5346-60. 2012
    ..These studies reveal a direct and dynamic role for glucocorticoids and GR signaling in the modulation of cardiomyocyte function...
  39. pmc Molecular mechanisms regulating glucocorticoid sensitivity and resistance
    Katherine L Gross
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Mol Cell Endocrinol 300:7-16. 2009
    ..The potential role of glucocorticoid receptor isoforms in determining cellular responsiveness to glucocorticoids is emphasized...
  40. ncbi request reprint Mechanisms of glucocorticoid receptor action in noninflammatory and inflammatory cells
    Brian M Necela
    Laboratory of Signal Transduction, Building 101, MD F3 07, National Institute of Environmental Health Sciences NIH, Research Triangle Park, NC 27709, USA
    Proc Am Thorac Soc 1:239-46. 2004
  41. pmc Essential role of stress hormone signaling in cardiomyocytes for the prevention of heart disease
    Robert H Oakley
    Laboratory of Signal Transduction, Comparative Medicine Branch, and Cellular and Molecular Pathology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709
    Proc Natl Acad Sci U S A 110:17035-40. 2013
    ..Moreover, our findings suggest that selective activation of cardiomyocyte GR may represent an approach for the prevention of heart disease. ..
  42. pmc Life and death of lymphocytes: a volume regulation affair
    Carl D Bortner
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, USA
    Cell Physiol Biochem 28:1079-88. 2011
    ..Here we review studies that suggest that AVD plays an active role during apoptosis and the underlying flux of ions that results in this morphological event regulates the programmed cell death process...
  43. ncbi request reprint The role of apoptotic volume decrease and ionic homeostasis in the activation and repression of apoptosis
    Carl D Bortner
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Pflugers Arch 448:313-8. 2004
    ..On going studies continue to support the hypothesis that the change in intracellular ions can alter a cells decision to die by apoptosis...
  44. ncbi request reprint The origin and functions of multiple human glucocorticoid receptor isoforms
    Nick Z Lu
    The Laboratory of Signal Transduction, Molecular Endocrinology Group, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Ann N Y Acad Sci 1024:102-23. 2004
    ..Unchecked expression of some isoforms, for example hGRbeta, has been implicated in various diseases...
  45. pmc Glucocorticoids and tumor necrosis factor alpha cooperatively regulate toll-like receptor 2 gene expression
    Marcela A Hermoso
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 24:4743-56. 2004
    ..Together, these studies define novel signaling mechanism for these three transcription factors, with a profound impact on discrimination of innate and adaptive immune responses...
  46. ncbi request reprint Mechanisms of glucocorticoid receptor signaling during inflammation
    Kathleen A Smoak
    Department of Health and Human Services, Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health, Building 101, Research Triangle Park, NC 27709, USA
    Mech Ageing Dev 125:697-706. 2004
    ..In this review, we discuss the molecular mechanisms underlying GR inhibition of inflammatory responses, with an emphasis on repression of NF-kappaB and AP-1 and their respective signaling pathways...
  47. ncbi request reprint Apoptosis and cell volume regulation: the importance of ions and ion channels
    Gerd Heimlich
    National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Adv Exp Med Biol 559:189-203. 2004
  48. ncbi request reprint A single amino acid change in the first zinc finger of the DNA binding domain of the glucocorticoid receptor regulates differential promoter selectivity
    Brian M Necela
    Laboratory of Signal Transduction, NIEHS, and Department of Health and Human Services, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:39279-88. 2004
    ..Our findings support a model by which the presence of a valine residue at position 437 of mGR induces a conformational change that leads to alterations in affinity and/or transcriptional activation in a promoter-dependent context...
  49. pmc Cell shrinkage and monovalent cation fluxes: role in apoptosis
    Carl D Bortner
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 462:176-88. 2007
    ..This review examines the relationship between this morphological change and intracellular monovalent ions during apoptosis...
  50. pmc Molecular determinants of glucocorticoid receptor mobility in living cells: the importance of ligand affinity
    Marcel J M Schaaf
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 23:1922-34. 2003
    ..In addition, this conformational change blocks immobilization of the receptor by MG132...
  51. pmc Glucocorticoids regulate arrestin gene expression and redirect the signaling profile of G protein-coupled receptors
    Robert H Oakley
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, US Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 109:17591-6. 2012
    ....
  52. pmc Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation
    Rodrigo Franco
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 282:30452-65. 2007
    ..These results document a necessary and critical role for GSH loss in apoptosis and clearly uncouple for the first time GSH depletion from ROS formation...
  53. ncbi request reprint Glucocorticoid-induced apoptosis of healthy and malignant lymphocytes
    Lindsay K Smith
    Molecular Endocrinology Group, Laboratory of Signal Transduction, NIEHS, NIH, DHHS, Research Triangle Park, NC, USA
    Prog Brain Res 182:1-30. 2010
    ..The development of novel therapies designed to overcome glucocorticoid resistance will dramatically improve the efficacy of glucocorticoid therapy in the treatment of haematological malignancies...
  54. pmc Cellular processing of the glucocorticoid receptor gene and protein: new mechanisms for generating tissue-specific actions of glucocorticoids
    Robert H Oakley
    Laboratory of Signal Transduction, NIEHS, National Institutes of Heath, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 286:3177-84. 2011
    ..Here, we discuss the origin and molecular properties of the GR isoforms and their contribution to the sensitivity and specificity of the glucocorticoid response...
  55. ncbi request reprint The physiology of human glucocorticoid receptor beta (hGRbeta) and glucocorticoid resistance
    Laura J Lewis-Tuffin
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 TW Alexander Drive, P O Box 12233, Research Triangle Park, NC 27709, USA
    Ann N Y Acad Sci 1069:1-9. 2006
    ..Higher ratios correlate with glucocorticoid sensitivity, while lower ratios correlate with glucocorticoid resistance. Thus hGRbeta can be an important modulator of glucocorticoid responsiveness...
  56. ncbi request reprint Estrogens and glucocorticoids have opposing effects on the amount and latent activity of complement proteins in the rat uterus
    Turk Rhen
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Biol Reprod 74:265-74. 2006
    ....
  57. doi request reprint Glucocorticoids regulate gene expression and repress cellular proliferation in human uterine leiomyoma cells
    Shannon Whirledge
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Horm Cancer 3:79-92. 2012
    ....
  58. pmc The five Rs of glucocorticoid action during inflammation: ready, reinforce, repress, resolve, and restore
    John M Busillo
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Trends Endocrinol Metab 24:109-19. 2013
    ..These ideas provide a framework by which glucocorticoids ready and reinforce the innate immune system, and repress the adaptive immune system, to help to resolve inflammation and restore homeostasis...
  59. doi request reprint Exploring the molecular mechanisms of glucocorticoid receptor action from sensitivity to resistance
    Sivapriya Ramamoorthy
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, N C, USA
    Endocr Dev 24:41-56. 2013
    ..Here, we summarize our current understanding of the mechanisms that modulate glucocorticoid sensitivity and resistance with a focus on GR-mediated signaling...
  60. ncbi request reprint Dexamethasone blocks the rapid biological effects of 17beta-estradiol in the rat uterus without antagonizing its global genomic actions
    Turk Rhen
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Dr, Research Triangle Park, North Carolina 27709, USA
    FASEB J 17:1849-70. 2003
    ..Together, these results indicate strong discord between the early biologic and genomic actions of estrogens and glucocorticoids and highlight a complex regulatory role for glucocorticoids and GR in the mammalian uterus...
  61. doi request reprint Emerging roles of glucocorticoid receptor phosphorylation in modulating glucocorticoid hormone action in health and disease
    Amy J Galliher-Beckley
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    IUBMB Life 61:979-86. 2009
    ..In doing so, we will review current knowledge about each phosphorylated residue within the GR and their contributions to modulating GC signaling in normal homeostatic physiology and during the progression of disease...
  62. pmc Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome
    John M Busillo
    Department of Health and Human Services, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 286:38703-13. 2011
    ..This effect was specific for glucocorticoids and dependent on the glucocorticoid receptor. These studies demonstrate a novel role for glucocorticoids in sensitizing the initial inflammatory response by the innate immune system...
  63. ncbi request reprint Cell volume and ion changes during apoptotic cell death
    Mireia Gómez-Angelats
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Adv Cancer Res 85:175-201. 2002
  64. ncbi request reprint International Union of Pharmacology. LXV. The pharmacology and classification of the nuclear receptor superfamily: glucocorticoid, mineralocorticoid, progesterone, and androgen receptors
    Nick Z Lu
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Pharmacol Rev 58:782-97. 2006
  65. doi request reprint Mechanisms generating diversity in glucocorticoid receptor signaling
    Javier R Revollo
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIEHS NIH, Research Triangle Park, North Carolina, USA
    Ann N Y Acad Sci 1179:167-78. 2009
    ..In this chapter, we review the biological and physiological implications of glucocorticoids, the GR, and many of the signal transduction mechanisms that mediate their action...
  66. pmc Tissue-specific glucocorticoid action: a family affair
    Katherine L Gross
    Molecular Endocrinology Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Trends Endocrinol Metab 19:331-9. 2008
    ..Furthermore, we evaluate the impact of tissue- and individual-specific diversity in the glucocorticoid pathway on human health and disease...
  67. ncbi request reprint Cellular mechanisms for the repression of apoptosis
    Carl D Bortner
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Annu Rev Pharmacol Toxicol 42:259-81. 2002
    ..In this review, we examine several modes of repression of apoptosis that exist in cells...
  68. ncbi request reprint The biology of the glucocorticoid receptor: new signaling mechanisms in health and disease
    Robert H Oakley
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC
    J Allergy Clin Immunol 132:1033-44. 2013
    ..Here we discuss the origin and molecular properties of the GR isoforms and their contribution to the specificity and sensitivity of glucocorticoid signaling in healthy and diseased tissues. ..
  69. ncbi request reprint The glucocorticoid receptor: coding a diversity of proteins and responses through a single gene
    Matthew R Yudt
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709 2233, USA
    Mol Endocrinol 16:1719-26. 2002
    ..We propose that the multiplicity of receptor forms have unique tissue- specific actions on the downstream biology providing a mechanism to create GR signaling networks...
  70. ncbi request reprint SLCO/OATP-like transport of glutathione in FasL-induced apoptosis: glutathione efflux is coupled to an organic anion exchange and is necessary for the progression of the execution phase of apoptosis
    Rodrigo Franco
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 281:29542-57. 2006
    ..Together these results suggest that GSH efflux during FasL-induced apoptosis is mediated by a SLCO/OATP-like transport mechanism that modulates the progression of the execution phase of apoptosis...
  71. ncbi request reprint Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes
    Se Te J Huang
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    FASEB J 16:825-32. 2002
    ..Mutation of T74 only partially impaired the ability of Bcl-2 to block glucocorticoid-induced apoptosis whereas mutation of S70 in Bcl-2 did not alter its ability to block glucocorticoid-induced apoptosis...
  72. ncbi request reprint Crystallization of the human glucocorticoid receptor ligand binding domain: a step towards selective glucocorticoids
    Brian M Necela
    Laboratory of Signal Transduction, Building 101, MD F3 07, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Trends Pharmacol Sci 24:58-61. 2003
    ..The GR ligand binding domain also has a steroid binding pocket that is distinct from other nuclear receptors and might explain its selectivity for glucocorticoids and its diversity of responses...
  73. ncbi request reprint Molecular interplay between ion channels and the regulation of apoptosis
    Mona A Razik
    Laboratory of Signal Transduction, Molecular Endocrinology Group, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Biol Res 35:203-7. 2002
    ..Understanding the components and steps involved in this intricate process can further our insight to diseases and reveal new approaches for therapeutic treatment...
  74. ncbi request reprint Putting the brake on inflammatory responses: the role of glucocorticoids
    Marcela A Hermoso
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    IUBMB Life 55:497-504. 2003
    ..In this article, we discuss the physiological and molecular interaction that occurs between GR and proinflammatory cytokines signaling pathways...
  75. ncbi request reprint Cell volume control and signal transduction in apoptosis
    Mireia Gómez-Angelats
    The Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 30:541-51. 2002
    ..In this review, we will focus on the importance of the apoptotic volume decrease in the context of signaling and modulation of programmed cell death...