C F Chignell

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Effect of magnetite particles on photoinduced and nonphotoinduced free radical processes in human erythrocytes
    C F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 68:598-601. 1998
  2. ncbi request reprint The photosensitizing potential of compact fluorescent vs incandescent light bulbs
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC, USA
    Photochem Photobiol 84:1291-3. 2008
  3. ncbi request reprint A photochemical study of cells loaded with 2',7'-dichlorofluorescin: implications for the detection of reactive oxygen species generated during UVA irradiation
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709 2233, USA
    Free Radic Biol Med 34:1029-34. 2003
  4. ncbi request reprint The effect of static magnetic fields on the photohemolysis of human erythrocytes by ketoprofen
    C F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 67:591-5. 1998
  5. ncbi request reprint Photocarcinogenesis in the Tg.AC mouse: lomefloxacin and 8-methoxypsoralen
    C F Chignell
    Laboratory of Pharmacology and Chemistry, ETP, NIEHS, NIH, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 77:77-80. 2003
  6. pmc Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.) 3: effect on human lens and retinal pigment epithelial cells
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, NC, USA
    Photochem Photobiol 83:938-43. 2007
  7. ncbi request reprint EPR studies of in vivo radical production by 3,3',5,5'-tetrabromobisphenol A (TBBPA) in the Sprague-Dawley rat
    C F Chignell
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Toxicol Appl Pharmacol 230:17-22. 2008
  8. ncbi request reprint Free radical reactions photosensitized by the human lens component, kynurenine: an EPR and spin trapping investigation
    K J Reszka
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, NC, USA
    Free Radic Biol Med 20:23-34. 1996
  9. ncbi request reprint Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.) 1. Berberine
    J J Inbaraj
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Chem Res Toxicol 14:1529-34. 2001
  10. ncbi request reprint Photosensitization by norfloxacin is a function of pH
    P Bilski
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 64:496-500. 1996

Detail Information

Publications67

  1. ncbi request reprint Effect of magnetite particles on photoinduced and nonphotoinduced free radical processes in human erythrocytes
    C F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 68:598-601. 1998
    ..These findings may be attributed to an increase in the membrane concentration of lipid radicals as a result of a magnetic field-induced increase in radicals escaping from triplet radical pairs...
  2. ncbi request reprint The photosensitizing potential of compact fluorescent vs incandescent light bulbs
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC, USA
    Photochem Photobiol 84:1291-3. 2008
    ..These results suggest that substitution of a compact fluorescent bulb for an incandescent bulb of the same luminous flux should not increase the phototoxicity of skin porphyrins or lens riboflavin...
  3. ncbi request reprint A photochemical study of cells loaded with 2',7'-dichlorofluorescin: implications for the detection of reactive oxygen species generated during UVA irradiation
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709 2233, USA
    Free Radic Biol Med 34:1029-34. 2003
    ..Our findings suggest that DCFH can not be used to detect the UVA-induced generation of reactive oxygen species in cells when the dye is present during exposure...
  4. ncbi request reprint The effect of static magnetic fields on the photohemolysis of human erythrocytes by ketoprofen
    C F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 67:591-5. 1998
    ....
  5. ncbi request reprint Photocarcinogenesis in the Tg.AC mouse: lomefloxacin and 8-methoxypsoralen
    C F Chignell
    Laboratory of Pharmacology and Chemistry, ETP, NIEHS, NIH, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 77:77-80. 2003
    ..01) than that in the other two groups. Our findings suggest that more studies need to be done before the Tg.AC mouse can be used with confidence to identify parenterally administered photocarcinogens...
  6. pmc Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.) 3: effect on human lens and retinal pigment epithelial cells
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, NC, USA
    Photochem Photobiol 83:938-43. 2007
    ..Our findings show that eyewashes and lotions derived from Goldenseal or containing berberine must be used with caution when the eyes are exposed to bright sunlight but that oral preparations are not likely to cause ocular phototoxicity...
  7. ncbi request reprint EPR studies of in vivo radical production by 3,3',5,5'-tetrabromobisphenol A (TBBPA) in the Sprague-Dawley rat
    C F Chignell
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Toxicol Appl Pharmacol 230:17-22. 2008
    ....
  8. ncbi request reprint Free radical reactions photosensitized by the human lens component, kynurenine: an EPR and spin trapping investigation
    K J Reszka
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, NC, USA
    Free Radic Biol Med 20:23-34. 1996
    ..We postulate that: (i) 3HKG may be able to photoinitiate free radical reactions in vivo, and (ii) oxygen is an important factor determining the yields of free radical processes initiated by lenticular chromophores...
  9. ncbi request reprint Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.) 1. Berberine
    J J Inbaraj
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Chem Res Toxicol 14:1529-34. 2001
    ..These findings suggest that exposure to sunlight or artificial light sources emitting UVA should be avoided when topical preparations derived from Goldenseal or containing berberine are used...
  10. ncbi request reprint Photosensitization by norfloxacin is a function of pH
    P Bilski
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 64:496-500. 1996
    ..The relatively high, unquenched 1O2 production at physiological pH 7.4 (phi s0 approximately 0.08) suggests that 1O2 reactions may play an important role in the cutaneous phototoxicity of norfloxacin and other FQ antibiotics...
  11. ncbi request reprint Influence of solvent polarity and proticity on the photochemical properties of norfloxacin
    P Bilski
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 68:20-4. 1998
    ..The phototoxicity of NF and other fluoroquinolone antibiotics may strongly depend on their localization in hydrophilic or hydrophobic cell/tissue regions...
  12. ncbi request reprint Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:364-9. 2002
    ..Western blot analysis demonstrates that myoglobin forms the predominant radical-derived nitrone adduct in rat heart supernatant...
  13. ncbi request reprint Acid-catalyzed oxidation of the anticancer agent mitoxantrone by nitrite ions
    K J Reszka
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 22709, USA
    Mol Pharmacol 50:1612-8. 1996
    ..Because these reactive forms of nitrogen may be present in vivo, this property of the drug may be relevant to its biological or perhaps anticancer activities...
  14. ncbi request reprint Lactoperoxidase-catalyzed oxidation of the anticancer agent mitoxantrone by nitrogen dioxide (NO2.) radicals
    K J Reszka
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences NIH, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 10:1325-30. 1997
    ..It is therefore possible that the ability of mitoxantrone to react with the nitrogen dioxide radical may be relevant to the biological action of the drug in vivo...
  15. ncbi request reprint Photochemistry and photocytotoxicity of alkaloids from Goldenseal (Hydrastis canadensis L.). 2. Palmatine, hydrastine, canadine, and hydrastinine
    J J Inbaraj
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 19:739-44. 2006
    ..Although palmatine is photoactive, it is present in such small amounts in Goldenseal root powder that the phototoxicity of the herb is most likely due to berberine, the major constituent alkaloid...
  16. ncbi request reprint Lactoperoxidase-catalyzed oxidation of melanin by reactive nitrogen species derived from nitrite (NO2-): an EPR study
    K J Reszka
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 25:208-16. 1998
    ..This property may be relevant to the physiological functions of the melanin pigments in vivo...
  17. ncbi request reprint Vitamin B6 (pyridoxine) and its derivatives are efficient singlet oxygen quenchers and potential fungal antioxidants
    P Bilski
    Laboratory of Pharmacology and Chemistry, NIEHS Environmental Toxicology Program, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 71:129-34. 2000
    ....
  18. ncbi request reprint Cytotoxic action of juglone and plumbagin: a mechanistic study using HaCaT keratinocytes
    J Johnson Inbaraj
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 17:55-62. 2004
    ..However, it is probable that the antifungal, antiviral, and antibacterial properties of these quinones are the result of redox cycling...
  19. ncbi request reprint Photochemical reactions involved in the phototoxicity of the anticonvulsant and antidepressant drug lamotrigine (Lamictal)
    Piotr J Bilski
    Laboratory of Pharmacology, NIEHS, RTP, NC, USA
    Photochem Photobiol 85:1327-35. 2009
    ..Our results demonstrate that LTG is a moderate generator of 1O2 prone to photodechlorination, especially in a reducing environment, which can contribute to the reported phototoxicity of LTG...
  20. ncbi request reprint Photochemical and photobiological studies of tirapazamine (SR 4233) and related quinoxaline 1,4-Di-N-oxide analogues
    J Johnson Inbaraj
    Laboratory of Pharmacology and Chemistry, NIEHS, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 16:164-70. 2003
    ..These studies show that the photochemistry of TPZ, TPZCN, and quindoxin is complex and depends on the redox environment and whether oxygen is present...
  21. pmc UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    Cancer Res 68:3752-8. 2008
    ..Identification of these signaling pathways in UVA-induced cell proliferation will facilitate the development of efficient and safe chemopreventive and therapeutic strategies for skin cancer...
  22. ncbi request reprint Photogeneration of fluoride by the fluoroquinolone antimicrobial agents lomefloxacin and fleroxacin
    L J Martinez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 65:599-602. 1997
    ..These findings may explain why lomefloxacin and fleroxacin are more photomutagenic and photocarcinogenic than fluoroquinolones in which C-8 is either unsubstituted or bears a substituent other than fluorine...
  23. ncbi request reprint Photochemical characterization of water samples from Minnesota and Vermont sites with malformed frogs: potential influence of photosensitization by singlet molecular oxygen (1O(2)) and free radicals on aquatic toxicity
    P Bilski
    Laboratory of Pharmacology and Chemistry and Laboratory of Toxicology, ETP, NIEHS, Research Triangle Park, NC 27709, USA
    Aquat Toxicol 65:229-41. 2003
    ..The observation of 1O(2) emission directly from natural water may also provide new opportunities to investigate the involvement of 1O(2) in other complex environmental processes...
  24. ncbi request reprint Merocyanine 540 solubilized as an ion pair with cationic surfactant in nonpolar solvents: spectral and photochemical properties
    P Bilski
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Photochem Photobiol 69:671-6. 1999
    ..In contrast, any cytotoxic products from photodecomposition may be important in hydrophilic cell compartments. Our data show the spectral and photochemical properties of MC in a pure hydrophobic environment...
  25. ncbi request reprint Interaction of singlet molecular oxygen with melatonin and related indoles
    Z Matuszak
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 78:449-55. 2003
    ....
  26. ncbi request reprint Interaction of singlet molecular oxygen with double fluorescent and spin sensors
    P Bilski
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC, USA
    Free Radic Biol Med 34:489-95. 2003
    ..This result shows that care must be taken when the dansyl-based sensors are used in experiments requiring UV irradiation. We hope that our results will contribute to a better characterization and wider use of these novel double sensors...
  27. ncbi request reprint Photocleavage of DNA by the fluoroquinolone antibacterials
    L Martinez
    Laboratory of Pharmacology and Chemistry, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Photochem Photobiol B 45:51-9. 1998
    ....
  28. ncbi request reprint Anthralin stimulates keratinocyte-derived proinflammatory cytokines via generation of reactive oxygen species
    R W Lange
    Environmental Immunology Section, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Inflamm Res 47:174-81. 1998
    ..The role of inflammatory cytokines and their induction by anthralin-derived reactive oxygen species were studied in cultures of normal human keratinocytes (NHKs)...
  29. ncbi request reprint Optimization of a pulse laser spectrometer for the measurement of the kinetics of singlet oxygen O2(delta 1g) decay in solution
    P Bilski
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Biochem Biophys Methods 33:73-80. 1996
    ..The small sample volume (0.1 ml) prepared in quartz tubes permits fast and inexpensive data acquisition for the determination of 1O2 quenching rate constants in most solvents...
  30. ncbi request reprint Cleavage of epidermal growth factor receptor by caspase during apoptosis is independent of its internalization
    Y Y He
    Laboratory of Pharmacology and Chemistry, NIEHS, National Institute of Health, Research Triangle Park, North Carolina 27709 2233, USA
    Oncogene 25:1521-31. 2006
    ..A cleavage-deficient EGFR mutant delayed apoptosis process. We conclude that the evolutionarily conserved C-terminus domain of EGFR is the target of caspases and subjected to degradation during apoptosis to shut down its signaling...
  31. ncbi request reprint Oxidation of biological electron donors and antioxidants by a reactive lactoperoxidase metabolite from nitrite (NO2-): an EPR and spin trapping study
    K J Reszka
    Department of Ophthalmology, Columbia University, New York, NY, USA
    Free Radic Biol Med 26:669-78. 1999
    ....
  32. ncbi request reprint Ultraviolet A sensitivity in Smith-Lemli-Opitz syndrome: Possible involvement of cholesta-5,7,9(11)-trien-3 beta-ol
    Colin F Chignell
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 41:339-46. 2006
    ..Finally, we have previously detected 9-DDHC in skin lipids from normal subjects, so this sterol may also be the skin chromophore responsible for skin photoaging and UV-induced skin cancer...
  33. pmc Phototoxicity in human retinal pigment epithelial cells promoted by hypericin, a component of St. John's wort
    Albert R Wielgus
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
    Photochem Photobiol 83:706-13. 2007
    ..Thus, ingested SJW is potentially phototoxic to the retina and could contribute to retinal or early macular degeneration...
  34. ncbi request reprint Role of phagocyte oxidase in UVA-induced oxidative stress and apoptosis in keratinocytes
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    J Invest Dermatol 125:560-6. 2005
    ....
  35. ncbi request reprint Limited protective role of V-PYRRO/NO against cholestasis produced by alpha-naphthylisothiocyanate in mice
    Jie Liu
    Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, NCI at NIEHS, Mail Drop F0 09, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Biochem Pharmacol 70:144-51. 2005
    ..Thus, the liver-selective NO donor, V-PYRRO/NO, was partially protective against ANIT-induced liver injury, without affecting ANIT-induced cholestasis and cholestasis-related gene expression...
  36. ncbi request reprint Photo-induced reactive oxygen species generation by different water-soluble fullerenes (C) and their cytotoxicity in human keratinocytes
    Baozhong Zhao
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC, USA
    Photochem Photobiol 84:1215-23. 2008
    ..In vitro tests with HaCaT keratinocytes provided evidence that (gamma-CyD)(2)/C(60) phototoxicity is mainly mediated by (1)O(2) (Type II mechanism) with only a minor contribution from free radicals (Type I mechanism)...
  37. pmc Pristine (C60) and hydroxylated [C60(OH)24] fullerene phototoxicity towards HaCaT keratinocytes: type I vs type II mechanisms
    Baozhong Zhao
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 21:1056-63. 2008
    ..Our results clearly show that the phototoxicity induced by (gamma-CyD) 2/C 60 is mainly mediated by singlet oxygen with a minor contribution from superoxide, while C 60(OH) 24 phototoxicity is mainly due to superoxide...
  38. ncbi request reprint Delayed and sustained activation of extracellular signal-regulated kinase in human keratinocytes by UVA: implications in carcinogenesis
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, FO 06, 111 Alexander Dr, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:53867-74. 2004
    ..The delayed and sustained ERK activation provides a survival signal to human HaCaT keratinocytes, which may serve as an important mechanism for cell transformation and potential skin carcinogenesis in vivo caused by UVA exposure...
  39. pmc Identification of protein-derived tyrosyl radical in the reaction of cytochrome c and hydrogen peroxide: characterization by ESR spin-trapping, HPLC and MS
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, U S A
    Biochem J 363:281-8. 2002
    ..We conclude that the cytochrome c-derived tyrosyl radical was trapped by MNP, leading to a persistent radical adduct at the C-3/C-5 positions of the tyrosine phenyl ring...
  40. pmc Photocytotoxicity of the fluorescent nonsteroidal androgen receptor ligand TDPQ
    Piotr J Bilski
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA
    Photochem Photobiol 85:1225-32. 2009
    ..The combination of AR selectivity with photocytotoxicity makes TDPQ a promising candidate for selective targeting of AR-positive cells during photodynamic therapy...
  41. pmc Immunological detection of N-formylkynurenine in oxidized proteins
    Marilyn Ehrenshaft
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 46:1260-6. 2009
    ..Results from mass spectrometric analysis of photooxidized myoglobin samples corroborate the immunological data, detecting an increase in NFK content as the extent of photooxidation increases...
  42. ncbi request reprint Role of reduced glutathione efflux in apoptosis of immortalized human keratinocytes induced by UVA
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:8058-64. 2003
    ..These provide targets for macrophage recognition and phagocytosis and thus minimize the potential to invoke inflammation or neoplastic transformation...
  43. ncbi request reprint Expression profiling of human keratinocyte response to ultraviolet A: implications in apoptosis
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    J Invest Dermatol 122:533-43. 2004
    ..This initial microarray analysis could advance our understanding of cellular responses to ultraviolet A exposure, and provide a platform from which to further study ultraviolet-A-induced apoptosis and carcinogenesis...
  44. ncbi request reprint Epidermal growth factor receptor down-regulation induced by UVA in human keratinocytes does not require the receptor kinase activity
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences NIH, Research Triangle Park, NC 27709, USA
    J Biol Chem 278:42457-65. 2003
    ....
  45. pmc Partial colocalization of oxidized, N-formylkynurenine-containing proteins in mitochondria and Golgi of keratinocytes
    Marilyn Ehrenshaft
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
    Photochem Photobiol 86:752-6. 2010
    ..These experiments demonstrate that we have developed a tool for the specific detection of oxidized tryptophan residues in cells and suggest that this tool could be useful in tracking the fate of these oxidized proteins...
  46. ncbi request reprint UVA-ketoprofen-induced hemoglobin radicals detected by immuno-spin trapping
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 77:585-91. 2003
    ..The successful detection of protein radical formation caused by KP photosensitization could help further understand the photoallergic effect of this NSAID...
  47. pmc Difference in phototoxicity of cyclodextrin complexed fullerene [(gamma-CyD)2/C60] and its aggregated derivatives toward human lens epithelial cells
    Baozhong Zhao
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 22:660-7. 2009
    ..Our results also demonstrate that UVA-blocking sunglasses can limit the ocular phototoxicity of this nanomaterial, while nontoxic endogenous antioxidants like lutein or Trolox cannot provide adequate protection...
  48. pmc An in vivo study of free radicals generated in murine skin by protoporphyrin IX and visible light
    Kozo Nakai
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
    Photochem Photobiol 82:738-40. 2006
    ..Our approach may be useful for the detection of free radicals generated by other skin photosensitizers and may also provide a means for testing putative skin-protecting agents...
  49. ncbi request reprint Photochemistry of naphthalene diimides: EPR study of free radical formation via photoredox process
    Krzysztof J Reszka
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC, USA
    Photochem Photobiol 81:573-80. 2005
    ..These observations could be pertinent to the application of naphthalene diimides as selective photonucleases, PDT anticancer agents or both...
  50. ncbi request reprint Low level, long-term inorganic arsenite exposure causes generalized resistance to apoptosis in cultured human keratinocytes: potential role in skin co-carcinogenesis
    Jingbo Pi
    Laboratory of Comparative Carcinogenesis, National Cancer Institute at the National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Int J Cancer 116:20-6. 2005
    ..The observation that As-TL cells show no lessening of UV-induced genotoxicity supports this possibility...
  51. ncbi request reprint Chronic UVA irradiation of human HaCaT keratinocytes induces malignant transformation associated with acquired apoptotic resistance
    Y Y He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Oncogene 25:3680-8. 2006
    ..Further comparisons between the transformed ULTH and control cells should lead to a better understanding of the mechanism of UVA carcinogenesis and may help identify biomarkers for UVA-induced skin malignancies...
  52. ncbi request reprint Photosensitized oxidation of 2',7'-dichlorofluorescin: singlet oxygen does not contribute to the formation of fluorescent oxidation product 2',7'-dichlorofluorescein
    P Bilski
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:938-46. 2002
    ..These findings suggest that DCFH should not be regarded as a probe sensitive to singlet molecular oxygen, and that care must be taken when using DCFH to measure oxidative stress in cells as a result of both visible and UV light exposure...
  53. pmc Spatial distribution of protein damage by singlet oxygen in keratinocytes
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
    Photochem Photobiol 84:69-74. 2008
    ..The subcellular localization of the protein DMPO adducts was coincident with that of RB. This approach may provide information on the spatial distribution of singlet oxygen generated in cells...
  54. ncbi request reprint Photophysical and photochemical studies of 2-phenylbenzimidazole and UVB sunscreen 2-phenylbenzimidazole-5-sulfonic acid
    J Johnson Inbaraj
    Laboratory of Pharmacology and Chemistry, NIEHS, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 75:107-16. 2002
    ..07 and 0.04 in MeCN for PBI and PBSA, respectively. These studies demonstrate that UV irradiation of PBSA and PBI generates a variety of free radicals and active oxygen species that may be involved in the photodamage of DNA...
  55. ncbi request reprint Phloxine B phototoxicity: a mechanistic study using HaCaT keratinocytes
    Johnson J Inbaraj
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 81:81-8. 2005
    ..Our findings suggest that concern is warranted about the use of this dye in cosmetic products, as a food additive and in insecticidal sprays...
  56. ncbi request reprint Direct detection of singlet oxygen via its phosphorescence from cellular and fungal cultures
    Piotr Bilski
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Methods Enzymol 352:41-52. 2002
    ..The model systems and the procedures described can be adopted for other studies involving 1O2 and oxidative stress in living cells...
  57. pmc Enhanced photodynamic efficacy towards melanoma cells by encapsulation of Pc4 in silica nanoparticles
    Baozhong Zhao
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
    Toxicol Appl Pharmacol 241:163-72. 2009
    ..We expect that modifying the surface of silicon nanoparticles encapsulating the photosensitizers with antibodies specific to melanoma cells will lead to even better early diagnosis and targeted treatment of melanoma in the future...
  58. doi request reprint Phototoxicity and cytotoxicity of fullerol in human retinal pigment epithelial cells
    Albert R Wielgus
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Toxicol Appl Pharmacol 242:79-90. 2010
    ..In conclusion, ocular exposure to fullerol, particularly in the presence of sunlight, may lead to retinal damage...
  59. ncbi request reprint The effect of short intermittent light exposures on the melatonin circadian rhythm and NMU-induced breast cancer in female F344/N rats
    G S Travlos
    Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 29:126-36. 2001
    ..This finding was unexpected and suggests that melatonin can be produced by an organ or tissue other than the pineal gland...
  60. doi request reprint Blue light induced A2E oxidation in rat eyes--experimental animal model of dry AMD
    A R Wielgus
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Photochem Photobiol Sci 9:1505-12. 2010
    ..This effect may partially explain the association between blue light induced retinal injury and macular degeneration...
  61. ncbi request reprint Spin trapping of nitric oxide by aci anions of nitroalkanes
    Krzysztof J Reszka
    Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, NC 27709, USA
    Nitric Oxide 10:53-9. 2004
    ..These results indicate that nitroalkanes could potentially be used as prototypes for development of *NO-specific spin traps suitable for EPR analysis...
  62. ncbi request reprint Phototoxicity in human lens epithelial cells promoted by St. John's Wort
    Yu Ying He
    Laboratory of Chemistry and Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 10023, USA
    Photochem Photobiol 80:583-6. 2004
    ..Thus, ingested SJW is potentially phototoxic to the eye and could contribute to early cataractogenesis. Precautions should be taken to protect the eye from intense sunlight while taking SJW...
  63. pmc Phototoxicity and cytotoxicity of fullerol in human lens epithelial cells
    Joan E Roberts
    Department of Natural Sciences, Fordham University, 113 West 60th Street, New York City, NY 10023, USA
    Toxicol Appl Pharmacol 228:49-58. 2008
    ..Before fullerols are used to deliver drugs to the eye, they should be tested for photo- and cytotoxicity in vivo...
  64. ncbi request reprint Location of anthralin radical generation in mouse skin by UV-A irradiation: an estimation using microscopic EPR spectral-spatial imaging
    Ken Ichiro Matsumoto
    Department of Biofunction Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
    Magn Reson Med 55:738-42. 2006
    ..This result was consistent with the finding that the proportions of the radical in the dermis and epidermis were about 15% and 85%, respectively...
  65. ncbi request reprint The role of A2E in prevention or enhancement of light damage in human retinal pigment epithelial cells
    Joan E Roberts
    Department of Natural Sciences, Fordham University, New York, NY 10023, USA
    Photochem Photobiol 75:184-90. 2002
    ..These results imply that the role of A2E is not simple and may change over the course of a lifetime. A2E itself may play a protective role in the young eye but a toxic role in older eyes...
  66. pmc Influence of dietary melatonin on photoreceptor survival in the rat retina: an ocular toxicity study
    Allan F Wiechmann
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Exp Eye Res 86:241-50. 2008
    ..Chronic exposure to natural or artificial light and simultaneous intake of melatonin may potentially contribute to a significant loss of photoreceptor cells in the aging retina...
  67. ncbi request reprint Simulated microgravity induced damage in human retinal pigment epithelial cells
    Joan E Roberts
    Department of Natural Sciences, Fordham University, New York, NY 10023, USA
    Mol Vis 12:633-8. 2006
    ..The goal of this study was to determine the potential damage to the human retina that may occur from weightlessness during space flight using simulated microgravity...