Pratip K Chattopadhyay

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint The use of quantum dot nanocrystals in multicolor flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010
  2. ncbi request reprint Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 81:456-66. 2012
  3. pmc A chromatic explosion: the development and future of multiparameter flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3015, USA
    Immunology 125:441-9. 2008
  4. pmc Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Cytometry A 77:614-22. 2010
  5. ncbi request reprint Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1-6. 2006
  6. pmc The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donor
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 114:5071-80. 2009
  7. pmc Amine-reactive dyes for dead cell discrimination in fixed samples
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Curr Protoc Cytom . 2010
  8. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
  9. pmc High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrow
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 113:2238-44. 2009
  10. pmc Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 73:1001-9. 2008

Detail Information

Publications30

  1. doi request reprint The use of quantum dot nanocrystals in multicolor flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010
    ..This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology...
  2. ncbi request reprint Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 81:456-66. 2012
    ....
  3. pmc A chromatic explosion: the development and future of multiparameter flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3015, USA
    Immunology 125:441-9. 2008
    ..Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity...
  4. pmc Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Cytometry A 77:614-22. 2010
    ..Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future...
  5. ncbi request reprint Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1-6. 2006
    ..Unlike other assays, this method allows simultaneous assessment of other cell phenotypes or functions, is compatible with downstream RNA-based assays and preserves cell viability. This protocol can be completed in 9 h...
  6. pmc The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donor
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 114:5071-80. 2009
    ....
  7. pmc Amine-reactive dyes for dead cell discrimination in fixed samples
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Curr Protoc Cytom . 2010
    ..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue...
  8. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
    ....
  9. pmc High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrow
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 113:2238-44. 2009
    ..These data suggest that concomitant examination of bone marrow specimens in patients with myeloid leukemias might yield more definitive information in the search for immunologic prognosticators of clinical outcome...
  10. pmc Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 73:1001-9. 2008
    ....
  11. pmc Superior T memory stem cell persistence supports long-lived T cell memory
    Enrico Lugli
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, 40, Convent Dr, Bethesda, Maryland 20892, USA
    J Clin Invest 123:594-9. 2013
    ..Thus, one mechanism for maintenance of long-term T cell memory derives from the unique homeostatic properties of TSCM cells. Vaccination strategies designed to elicit durable cellular immunity should target the generation of TSCM cells...
  12. pmc The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
    J Leukoc Biol 85:88-97. 2009
    ..Thus, the use of CD57 provides a means to easily isolate viable cells with high cytolytic potential, without the need for lethal fixation/permeabilization techniques...
  13. pmc Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infection
    Takuya Yamamoto
    National Institutes of Health, Bethesda, MD, USA
    Blood 117:4805-15. 2011
    ..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons...
  14. pmc Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
    J Immunol 183:1120-32. 2009
    ..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection...
  15. pmc Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays
    Nima Aghaeepour
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada
    Bioinformatics 28:1009-16. 2012
    ..Thus, the value of PFC as a discovery tool is largely wasted...
  16. doi request reprint Quality assurance for polychromatic flow cytometry using a suite of calibration beads
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD, USA
    Nat Protoc 7:2067-79. 2012
    ..In sum, the procedures presented here represent an updated framework for optimizing, calibrating and standardizing a flow cytometer for daily use...
  17. pmc Cytometry: today's technology and tomorrow's horizons
    Pratip K Chattopadhyay
    Immunotechnology Section, VRC, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20817, USA
    Methods 57:251-8. 2012
    ..Ongoing efforts are aimed at algorithms to analyse these aggregated datasaets over large numbers of samples. Here we review the development efforts heralding the next stage of flow cytometry...
  18. ncbi request reprint Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
    Stephen P Perfetto
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
    J Immunol Methods 313:199-208. 2006
    ..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments...
  19. pmc Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infection
    David A Price
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:923-36. 2009
    ..Thus, the pattern of antigen-specific clonotype recruitment within a protective CD8(+) T cell population is a prognostic indicator of vaccine efficacy and biological outcome in an AIDS virus infection...
  20. doi request reprint Immunophenotyping of T cell subpopulations in HIV disease
    Pratip K Chattopadhyay
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2005
    ....
  21. pmc Highly multiplexed quantitation of gene expression on single cells
    Maria H Dominguez
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, United States
    J Immunol Methods 391:133-45. 2013
    ....
  22. pmc Holoendemic malaria exposure is associated with altered Epstein-Barr virus-specific CD8(+) T-cell differentiation
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 87:1779-88. 2013
    ..These observations define a malaria-associated aberration localized to the EBV-specific CD8(+) T-cell compartment that illuminates the etiology of eBL...
  23. ncbi request reprint Application of quantum dots to multicolor flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Methods Mol Biol 374:175-84. 2007
    ..Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments...
  24. doi request reprint Quantum dot technology in flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Methods Cell Biol 102:463-77. 2011
    ....
  25. pmc Immunologic and virologic events in early HIV infection predict subsequent rate of progression
    Anuradha Ganesan
    National Naval Medical Center, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, Maryland 20892, USA
    J Infect Dis 201:272-84. 2010
    ..Because central memory T (T(CM)) cells play a critical role in the pathogenesis of simian immunodeficiency virus disease, we hypothesized that quantifying these cells in early HIV infection could provide prognostic information...
  26. ncbi request reprint A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5509, Bethesda, Maryland 20892, USA
    Nat Med 11:1113-7. 2005
    ..For vaccine- or pathogen-specific responses, we found substantial heterogeneity in expression of CD154 and cytokines, suggesting previously unrecognized diversity in abilities of responding cells to stimulate APCs through CD40...
  27. ncbi request reprint Seventeen-colour flow cytometry: unravelling the immune system
    Stephen P Perfetto
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892 3015, United States
    Nat Rev Immunol 4:648-55. 2004
  28. pmc Detection of low avidity CD8(+) T cell populations with coreceptor-enhanced peptide-major histocompatibility complex class I tetramers
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol Methods 338:31-9. 2008
    ....
  29. pmc Longitudinal assessment of de novo T cell production in relation to HIV-associated T cell homeostasis failure
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    AIDS Res Hum Retroviruses 22:501-7. 2006
    ..These results suggest that deficits in de novo T cell production, either through the decline of thymic function or the destruction of naive T cells, are likely to play an important role in TCH failure and progression of HIV-1 disease...
  30. doi request reprint Single-cell technologies for monitoring immune systems
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, Maryland, USA
    Nat Immunol 15:128-35. 2014
    ..We summarize recent approaches to making such computations tractable and discuss challenges for integrating heterogeneous data obtained using these single-cell technologies. ..