Research Topics
Species | Pratip K ChattopadhyaySummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
The use of quantum dot nanocrystals in multicolor flow cytometryPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010..This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology...- Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experimentsPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
Cytometry A 81:456-66. 2012.... 
Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV diseasePratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Cytometry A 77:614-22. 2010..Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future...- A chromatic explosion: the development and future of multiparameter flow cytometryPratip K Chattopadhyay
Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3015, USA
Immunology 125:441-9. 2008..Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity... 
Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expressionPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Protoc 1:1-6. 2006..Unlike other assays, this method allows simultaneous assessment of other cell phenotypes or functions, is compatible with downstream RNA-based assays and preserves cell viability. This protocol can be completed in 9 h...- The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donorPhillip Scheinberg
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Blood 114:5071-80. 2009.... - Amine-reactive dyes for dead cell discrimination in fixed samplesStephen P Perfetto
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
Curr Protoc Cytom . 2010..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue... - Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometryPratip K Chattopadhyay
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Med 12:972-7. 2006.... - High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrowJ Joseph Melenhorst
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 113:2238-44. 2009..These data suggest that concomitant examination of bone marrow specimens in patients with myeloid leukemias might yield more definitive information in the search for immunologic prognosticators of clinical outcome... - Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramersPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cytometry A 73:1001-9. 2008.... - Superior T memory stem cell persistence supports long-lived T cell memoryEnrico Lugli
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, 40, Convent Dr, Bethesda, Maryland 20892, USA
J Clin Invest 123:594-9. 2013..Thus, one mechanism for maintenance of long-term T cell memory derives from the unique homeostatic properties of TSCM cells. Vaccination strategies designed to elicit durable cellular immunity should target the generation of TSCM cells... - The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expressionPratip K Chattopadhyay
Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
J Leukoc Biol 85:88-97. 2009..Thus, the use of CD57 provides a means to easily isolate viable cells with high cytolytic potential, without the need for lethal fixation/permeabilization techniques... - Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infectionTakuya Yamamoto
National Institutes of Health, Bethesda, MD, USA
Blood 117:4805-15. 2011..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons... - Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
J Immunol 183:1120-32. 2009..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection... - Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assaysNima Aghaeepour
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada
Bioinformatics 28:1009-16. 2012..Thus, the value of PFC as a discovery tool is largely wasted... - Quality assurance for polychromatic flow cytometry using a suite of calibration beadsStephen P Perfetto
Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD, USA
Nat Protoc 7:2067-79. 2012..In sum, the procedures presented here represent an updated framework for optimizing, calibrating and standardizing a flow cytometer for daily use... - Cytometry: today's technology and tomorrow's horizonsPratip K Chattopadhyay
Immunotechnology Section, VRC, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20817, USA
Methods 57:251-8. 2012..Ongoing efforts are aimed at algorithms to analyse these aggregated datasaets over large numbers of samples. Here we review the development efforts heralding the next stage of flow cytometry... - Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometryStephen P Perfetto
Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
J Immunol Methods 313:199-208. 2006..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments... - Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infectionDavid A Price
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 206:923-36. 2009..Thus, the pattern of antigen-specific clonotype recruitment within a protective CD8(+) T cell population is a prognostic indicator of vaccine efficacy and biological outcome in an AIDS virus infection... - Immunophenotyping of T cell subpopulations in HIV diseasePratip K Chattopadhyay
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Immunol . 2005.... - Holoendemic malaria exposure is associated with altered Epstein-Barr virus-specific CD8(+) T-cell differentiationPratip K Chattopadhyay
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
J Virol 87:1779-88. 2013..These observations define a malaria-associated aberration localized to the EBV-specific CD8(+) T-cell compartment that illuminates the etiology of eBL... - Application of quantum dots to multicolor flow cytometryPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Methods Mol Biol 374:175-84. 2007..Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments... - Quantum dot technology in flow cytometryPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
Methods Cell Biol 102:463-77. 2011.... - Immunologic and virologic events in early HIV infection predict subsequent rate of progressionAnuradha Ganesan
National Naval Medical Center, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, Maryland 20892, USA
J Infect Dis 201:272-84. 2010..Because central memory T (T(CM)) cells play a critical role in the pathogenesis of simian immunodeficiency virus disease, we hypothesized that quantifying these cells in early HIV infection could provide prognostic information... - A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profilesPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5509, Bethesda, Maryland 20892, USA
Nat Med 11:1113-7. 2005..For vaccine- or pathogen-specific responses, we found substantial heterogeneity in expression of CD154 and cytokines, suggesting previously unrecognized diversity in abilities of responding cells to stimulate APCs through CD40... - Highly multiplexed quantitation of gene expression on single cellsMaria H Dominguez
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, United States
J Immunol Methods 391:133-45. 2013.... - Seventeen-colour flow cytometry: unravelling the immune systemStephen P Perfetto
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892-3015, United States
Nat Rev Immunol 4:648-55. 2004 - Detection of low avidity CD8(+) T cell populations with coreceptor-enhanced peptide-major histocompatibility complex class I tetramersJ Joseph Melenhorst
Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol Methods 338:31-9. 2008.... - Longitudinal assessment of de novo T cell production in relation to HIV-associated T cell homeostasis failurePratip K Chattopadhyay
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
AIDS Res Hum Retroviruses 22:501-7. 2006..These results suggest that deficits in de novo T cell production, either through the decline of thymic function or the destruction of naive T cells, are likely to play an important role in TCH failure and progression of HIV-1 disease...