Research Topics
Genomes and Genes
| Wai Yee ChanSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cellsShane W Oram
Department of Medicine, VA Medical Center, University of California, San Francisco, USA
BMC Cancer 6:154. 2006..TSPY contains a SET/NAP domain that is present in a family of cyclin B and/or histone binding proteins represented by the oncoprotein SET and the nucleosome assembly protein 1 (NAP1), involved in cell cycle regulation and replication...
The complexity of antisense transcription revealed by the study of developing male germ cellsWai Yee Chan
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
Genomics 87:681-92. 2006..Thus antisense transcripts have complex origins and structures and the sense and antisense transcripts can be regulated independently...- Transcriptome analyses of male germ cells with serial analysis of gene expression (SAGE)Wai Yee Chan
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
Mol Cell Endocrinol 250:8-19. 2006..A number of genes were shown to undergo differential splicing during spermatogenesis giving rise to cell-specific splice variants... - Disorders of sexual development caused by luteinizing hormone receptor mutationsWai Yee Chan
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
Beijing Da Xue Xue Bao 37:32-8. 2005..Molecular diagnosis of the disorders caused by mutation of the LHR can be achieved by direct sequencing of the LHR gene... - Expression profiling of purified male germ cells: stage-specific expression patterns related to meiosis and postmeiotic developmentAlan L Y Pang
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
Physiol Genomics 24:75-85. 2006..We also provide evidence that underscores the advantage of using purified germ cells over whole testes in profiling spermatogenic gene expression to identify transcripts that demonstrate stage-specific expression patterns... - Identification of differentially expressed genes in mouse spermatogenesisAlan L Y Pang
Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, NIH, USA
J Androl 24:899-911. 2003..Many of the genes identified were not previously characterized. This study highlights new targets for manipulation to unravel the molecular mechanism of spermatogenesis... - GonadSAGE: a comprehensive SAGE database for transcript discovery on male embryonic gonad developmentTin Lap Lee
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Bioinformatics 26:585-6. 2010..It represents an integrated platform that leads to a better understanding of male gonad development, and allows discovery of related novel targets and regulatory pathways... - Developmental staging of male murine embryonic gonad by SAGE analysisTin Lap Lee
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Genet Genomics 36:215-27. 2009..These regions may represent markers for early diagnosis for disorders of male gonad development as well as potential treatment targets... - Structural characterization and expression studies of Dby and its homologs in the mouseQueenie P Vong
Laboratory of Clinical Genomics, NICHD NIH, MSC 4429, Bethesda, MD 20892 4429, USA
J Androl 27:653-61. 2006..These observations indicate that unlike DBY in humans, the role of Dby in spermatogenesis is less obvious in the mouse and its biologic activity may be replaced by that of Ddx3 and D1Pas1... - Genomic landscape of developing male germ cellsTin Lap Lee
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
Birth Defects Res C Embryo Today 87:43-63. 2009..It is anticipated that application of systems biology approaches will further our understanding of the regulatory mechanism of spermatogenesis... - Application of transcriptional and biological network analyses in mouse germ-cell transcriptomesTin Lap Lee
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2C08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
Genomics 88:18-33. 2006..Taken together, our approach is reliable and provides a foundation for the generation of novel biological hypotheses for studying spermatogenesis... - Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: biological significanceShao-Ming Wu
Laboratory of Clinical Genomics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
Genomics 84:971-81. 2004..The databases generated by this work provide very useful resources for cellular localization of genes in silico. They are also extremely useful as sources for identification of splice variants of genes in germ cells... - Expression of human NAA11 (ARD1B) gene is tissue-specific and is regulated by DNA methylationAlan L Y Pang
Section on Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD, USA
Epigenetics 6:1391-9. 2011..Taken together, our results indicate NAA11 expression is tissue-specific and is epigenetically regulated by DNA methylation... 
Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridizationHoi Hung Cheung
Section on Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA
Methods Mol Biol 825:115-26. 2012..Here, we describe an established MeDIP protocol and tiling array hybridization method for profiling methylation of testicular germ cells...- Methylation patterns of Brahma during spermatogenesis and oogenesis: potential implicationsSohan R Nagrani
Laboratory of Clinical and Developmental Genomics, Program in Reproductive and Adult Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20814, USA
Fertil Steril 95:382-4. 2011..As the degree of methylation increases, the expression decreases. The change in methylation is opposite during oogenesis, which suggests opposite expression levels... - DNA methylation of cancer genomeHoi Hung Cheung
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Birth Defects Res C Embryo Today 87:335-50. 2009.... - Biological effect of a novel mutation in the third leucine-rich repeat of human luteinizing hormone receptorMichael Yiu Kwong Leung
Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, Maryland 20892 4429, USA
Mol Endocrinol 20:2493-503. 2006..This mutation might also affect an LHR-dimer interaction. Thus, the I114F mutation reduces ligand binding and signal transduction by the hLHR, and it is partially responsible for Leydig cell hypoplasia in the patient... - Cloning, characterization, and expression analysis of the novel acetyltransferase retrogene Ard1b in the mouseAlan Lap Yin Pang
Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Biol Reprod 81:302-9. 2009..Our results suggest that ARD1B may have an important role in the later course of the spermatogenic process... - Accessing the genomic effects of naked nanoceria in murine neuronal cellsTin Lap Lee
Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Nanomedicine 8:599-608. 2012..This team of investigators demonstrated the genomic effects of nanoceria, showing that it induced chemical- and size-specific changes in the murine neuronal cell transcriptome... - Molecular aspects of sex differentiationWai Yee Chan
Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, NIH, Bethesda, MD, USA
Curr Mol Med 2:25-37. 2002..Wider application of functional genomic techniques and introduction of proteomic analyses are expected to shed light to our understanding of this complicated developmental process... - GermSAGE: a comprehensive SAGE database for transcript discovery on male germ cell developmentTin Lap Lee
Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Nucleic Acids Res 37:D891-7. 2009..GermSAGE is freely available at http://germsage.nichd.nih.gov/.. - Human chorionic gonadotropin induces neuronal differentiation of PC12 cells through activation of stably expressed lutropin/choriogonadotropin receptorXing Li Meng
Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
Endocrinology 148:5865-73. 2007..These findings imply a potential role for hCG/LH and LH/CG-R in the development, maintenance, and regeneration of the mammalian nervous system, and in the neuropathogenesis of genetic diseases caused by a mutated LH/CG-R... - Evidence for genetic susceptibility to thrombosis in idiopathic intracranial hypertensionCigdem F Dogulu
Laboratory of Clinical Genomics, National Institute of Child Health and Development, National Institute of Health, MSC 4429, Bethesda, MD 20892-4429, USA
Thromb Res 111:389-95. 2003 - Metastasis-associated gene expression profile of liver and subcutaneous lesions derived from mouse pheochromocytoma cellsShoichiro Ohta
Reproductive Biology and Medicine Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 1109, USA
Mol Carcinog 47:245-51. 2008.... - A novel missense homozygous inactivating mutation in the fourth transmembrane helix of the luteinizing hormone receptor in leydig cell hypoplasiaMichael Yiu-Kwong Leung
Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 50892-4429, USA
Am J Med Genet A 130:146-53. 2004..Receptor trafficking was not affected by the mutation when the green fluorescence protein conjugated mutated receptor was expressed in HEK293 cells. The mutation caused inactivation of the hLHR and resulted in LCH in the patient... - Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closureJacob D Brown
Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:1462-7. 2009..We also identify misregulation of pax2 in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstream of the Pax2 pathway in directing proper closure of the optic fissure... - Serial analysis of gene expression in adrenocortical hyperplasia caused by a germline PRKAR1A mutationAnelia Horvath
Section on Endocrinology and Genetics, Developmental Endocrinology Branch, National Institute of Child Health and Human Development/NIH, Building 10, Clinical Research Center, Room I-1330, 10 Center Drive, MSC 1103, Bethesda, MD 20892-1103, USA
J Clin Endocrinol Metab 91:584-96. 2006..CONCLUSIONS: This study was the first of its kind for adrenal tissue and provides important information about the adrenal transcriptome and aberrant signaling in an inherited form of adrenocortical hyperplasia... - Downregulation of metastasis suppressor genes in malignant pheochromocytomaShoichiro Ohta
Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892-1583, USA
Int J Cancer 114:139-43. 2005..Thus, we conclude that altered function of these metastasis suppressor gene pathways may play an important role in the malignant behavior of pheochromocytoma... - A novel function of differentiation revealed by cDNA microarray profiling of p75NTR-regulated gene expressionAngele Nalbandian
Department of Cell Biology, Georgetown University Medical Center, Medical Dental Building, Washington, DC 20057 1436, USA
Differentiation 73:385-96. 2005.... - Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertilityZuping He
Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, District of Columbia 20057, USA
Reproduction 132:11-9. 2006..Moreover, issues pertaining to measurement reproducibility are highlighted for the application of microarray data to male infertility... - Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male miceYing Liu
Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
Mol Endocrinol 19:2380-9. 2005.... - Variable presentation of precocious puberty associated with the D564G mutation of the LHCGR gene in children with testotoxicosisGeorge S Jeha
Department of Pediatrics, Baylor College of Medicine and Texas Children s Hospital, Houston, Texas 77030, USA
J Pediatr 149:271-4. 2006..This report underscores the possibility that the effects of the mutant luteinizing hormone/choriogonadotropin receptor on phenotypic expression of FMPP, such as adult final height, are modified by other factors... - The Y-encoded TSPY protein: a significant marker potentially plays a role in the pathogenesis of testicular germ cell tumorsYunmin Li
Department of Medicine, VA Medical Center, University of California, San Francisco, CA 94121, USA
Hum Pathol 38:1470-81. 2007..Our results suggest that TSPY, in combination with other markers, could be an important marker for diagnosis and subclassification of TGCTs and support its role in the pathogenesis of both gonadoblastoma and TGCTs...