Wai Yee Chan

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc GermSAGE: a comprehensive SAGE database for transcript discovery on male germ cell development
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 37:D891-7. 2009
  2. pmc TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells
    Shane W Oram
    Department of Medicine, VA Medical Center, University of California, San Francisco, USA
    BMC Cancer 6:154. 2006
  3. ncbi request reprint The complexity of antisense transcription revealed by the study of developing male germ cells
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Genomics 87:681-92. 2006
  4. ncbi request reprint Transcriptome analyses of male germ cells with serial analysis of gene expression (SAGE)
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Mol Cell Endocrinol 250:8-19. 2006
  5. ncbi request reprint Disorders of sexual development caused by luteinizing hormone receptor mutations
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
    Beijing Da Xue Xue Bao 37:32-8. 2005
  6. ncbi request reprint Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: biological significance
    Shao Ming Wu
    Laboratory of Clinical Genomics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    Genomics 84:971-81. 2004
  7. ncbi request reprint Expression profiling of purified male germ cells: stage-specific expression patterns related to meiosis and postmeiotic development
    Alan L Y Pang
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
    Physiol Genomics 24:75-85. 2006
  8. doi request reprint Mapping genomic features of tiling microarray data by TileMapper
    Hoi Hung Cheung
    Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 1067:225-33. 2013
  9. pmc GonadSAGE: a comprehensive SAGE database for transcript discovery on male embryonic gonad development
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Bioinformatics 26:585-6. 2010
  10. ncbi request reprint Identification of differentially expressed genes in mouse spermatogenesis
    Alan L Y Pang
    Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, NIH, USA
    J Androl 24:899-911. 2003

Collaborators

Detail Information

Publications36

  1. pmc GermSAGE: a comprehensive SAGE database for transcript discovery on male germ cell development
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 37:D891-7. 2009
    ..GermSAGE is freely available at http://germsage.nichd.nih.gov/..
  2. pmc TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells
    Shane W Oram
    Department of Medicine, VA Medical Center, University of California, San Francisco, USA
    BMC Cancer 6:154. 2006
    ..TSPY contains a SET/NAP domain that is present in a family of cyclin B and/or histone binding proteins represented by the oncoprotein SET and the nucleosome assembly protein 1 (NAP1), involved in cell cycle regulation and replication...
  3. ncbi request reprint The complexity of antisense transcription revealed by the study of developing male germ cells
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Genomics 87:681-92. 2006
    ..Thus antisense transcripts have complex origins and structures and the sense and antisense transcripts can be regulated independently...
  4. ncbi request reprint Transcriptome analyses of male germ cells with serial analysis of gene expression (SAGE)
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Mol Cell Endocrinol 250:8-19. 2006
    ..A number of genes were shown to undergo differential splicing during spermatogenesis giving rise to cell-specific splice variants...
  5. ncbi request reprint Disorders of sexual development caused by luteinizing hormone receptor mutations
    Wai Yee Chan
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
    Beijing Da Xue Xue Bao 37:32-8. 2005
    ..Molecular diagnosis of the disorders caused by mutation of the LHR can be achieved by direct sequencing of the LHR gene...
  6. ncbi request reprint Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: biological significance
    Shao Ming Wu
    Laboratory of Clinical Genomics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    Genomics 84:971-81. 2004
    ..The databases generated by this work provide very useful resources for cellular localization of genes in silico. They are also extremely useful as sources for identification of splice variants of genes in germ cells...
  7. ncbi request reprint Expression profiling of purified male germ cells: stage-specific expression patterns related to meiosis and postmeiotic development
    Alan L Y Pang
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
    Physiol Genomics 24:75-85. 2006
    ..We also provide evidence that underscores the advantage of using purified germ cells over whole testes in profiling spermatogenic gene expression to identify transcripts that demonstrate stage-specific expression patterns...
  8. doi request reprint Mapping genomic features of tiling microarray data by TileMapper
    Hoi Hung Cheung
    Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 1067:225-33. 2013
    ..The outputs are saved in tabulated format, which permit flexible and simple processing in spreadsheet software, or to be exported to other pipelines for subsequent analysis. ..
  9. pmc GonadSAGE: a comprehensive SAGE database for transcript discovery on male embryonic gonad development
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Bioinformatics 26:585-6. 2010
    ..It represents an integrated platform that leads to a better understanding of male gonad development, and allows discovery of related novel targets and regulatory pathways...
  10. ncbi request reprint Identification of differentially expressed genes in mouse spermatogenesis
    Alan L Y Pang
    Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, NIH, USA
    J Androl 24:899-911. 2003
    ..Many of the genes identified were not previously characterized. This study highlights new targets for manipulation to unravel the molecular mechanism of spermatogenesis...
  11. ncbi request reprint A novel missense homozygous inactivating mutation in the fourth transmembrane helix of the luteinizing hormone receptor in leydig cell hypoplasia
    Michael Yiu Kwong Leung
    Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 50892 4429, USA
    Am J Med Genet A 130:146-53. 2004
    ..Receptor trafficking was not affected by the mutation when the green fluorescence protein conjugated mutated receptor was expressed in HEK293 cells. The mutation caused inactivation of the hLHR and resulted in LCH in the patient...
  12. pmc Developmental staging of male murine embryonic gonad by SAGE analysis
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Genet Genomics 36:215-27. 2009
    ..These regions may represent markers for early diagnosis for disorders of male gonad development as well as potential treatment targets...
  13. pmc Long-term vitamin A deficiency induces alteration of adult mouse spermatogenesis and spermatogonial differentiation: direct effect on spermatogonial gene expression and indirect effects via somatic cells
    Catherine Boucheron-Houston
    Laboratory of Clinical Genomics, Section on Developmental Genomics, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 4429, USA
    J Nutr Biochem 24:1123-35. 2013
    ....
  14. pmc Genomic landscape of developing male germ cells
    Tin Lap Lee
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 4429, USA
    Birth Defects Res C Embryo Today 87:43-63. 2009
    ..It is anticipated that application of systems biology approaches will further our understanding of the regulatory mechanism of spermatogenesis...
  15. ncbi request reprint Application of transcriptional and biological network analyses in mouse germ-cell transcriptomes
    Tin Lap Lee
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2C08, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Genomics 88:18-33. 2006
    ..Taken together, our approach is reliable and provides a foundation for the generation of novel biological hypotheses for studying spermatogenesis...
  16. ncbi request reprint Structural characterization and expression studies of Dby and its homologs in the mouse
    Queenie P Vong
    Laboratory of Clinical Genomics, NICHD NIH, MSC 4429, Bethesda, MD 20892 4429, USA
    J Androl 27:653-61. 2006
    ..These observations indicate that unlike DBY in humans, the role of Dby in spermatogenesis is less obvious in the mouse and its biologic activity may be replaced by that of Ddx3 and D1Pas1...
  17. ncbi request reprint Downregulation of metastasis suppressor genes in malignant pheochromocytoma
    Shoichiro Ohta
    Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892 1583, USA
    Int J Cancer 114:139-43. 2005
    ..Thus, we conclude that altered function of these metastasis suppressor gene pathways may play an important role in the malignant behavior of pheochromocytoma...
  18. pmc Expression of human NAA11 (ARD1B) gene is tissue-specific and is regulated by DNA methylation
    Alan L Y Pang
    Section on Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD, USA
    Epigenetics 6:1391-9. 2011
    ..Taken together, our results indicate NAA11 expression is tissue-specific and is epigenetically regulated by DNA methylation...
  19. pmc Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridization
    Hoi Hung Cheung
    Section on Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA
    Methods Mol Biol 825:115-26. 2012
    ..Here, we describe an established MeDIP protocol and tiling array hybridization method for profiling methylation of testicular germ cells...
  20. pmc Cloning, characterization, and expression analysis of the novel acetyltransferase retrogene Ard1b in the mouse
    Alan Lap Yin Pang
    Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biol Reprod 81:302-9. 2009
    ..Our results suggest that ARD1B may have an important role in the later course of the spermatogenic process...
  21. pmc Methylation patterns of Brahma during spermatogenesis and oogenesis: potential implications
    Sohan R Nagrani
    Laboratory of Clinical and Developmental Genomics, Program in Reproductive and Adult Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20814, USA
    Fertil Steril 95:382-4. 2011
    ..As the degree of methylation increases, the expression decreases. The change in methylation is opposite during oogenesis, which suggests opposite expression levels...
  22. pmc DNA methylation of cancer genome
    Hoi Hung Cheung
    Section on Developmental Genomics, Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Birth Defects Res C Embryo Today 87:335-50. 2009
    ....
  23. ncbi request reprint Evidence for genetic susceptibility to thrombosis in idiopathic intracranial hypertension
    Cigdem F Dogulu
    Laboratory of Clinical Genomics, National Institute of Child Health and Development, National Institute of Health, MSC 4429, Bethesda, MD 20892 4429, USA
    Thromb Res 111:389-95. 2003
  24. ncbi request reprint Biological effect of a novel mutation in the third leucine-rich repeat of human luteinizing hormone receptor
    Michael Yiu Kwong Leung
    Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, Building 49, Room 2A08, 49 Convent Drive, MSC 4429, Bethesda, Maryland 20892 4429, USA
    Mol Endocrinol 20:2493-503. 2006
    ..This mutation might also affect an LHR-dimer interaction. Thus, the I114F mutation reduces ligand binding and signal transduction by the hLHR, and it is partially responsible for Leydig cell hypoplasia in the patient...
  25. pmc Accessing the genomic effects of naked nanoceria in murine neuronal cells
    Tin Lap Lee
    Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nanomedicine 8:599-608. 2012
    ..These observations suggest that an in-depth assessment of potential health effects of naked nanoceria and other naked nanoparticles is both necessary and imminent...
  26. ncbi request reprint Human chorionic gonadotropin induces neuronal differentiation of PC12 cells through activation of stably expressed lutropin/choriogonadotropin receptor
    Xing Li Meng
    Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, MSC 4429, Bethesda, MD 20892 4429, USA
    Endocrinology 148:5865-73. 2007
    ..These findings imply a potential role for hCG/LH and LH/CG-R in the development, maintenance, and regeneration of the mammalian nervous system, and in the neuropathogenesis of genetic diseases caused by a mutated LH/CG-R...
  27. ncbi request reprint Molecular aspects of sex differentiation
    Wai Yee Chan
    Section on Developmental Genomics, Laboratory of Clinical Genomics, National Institute of Child Health and Human Development, NIH, Bethesda, MD, USA
    Curr Mol Med 2:25-37. 2002
    ..Wider application of functional genomic techniques and introduction of proteomic analyses are expected to shed light to our understanding of this complicated developmental process...
  28. pmc Up-regulation of proliferative and migratory genes in regulatory T cells from patients with metastatic castration-resistant prostate cancer
    Ngar Yee Huen
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Cancer 133:373-82. 2013
    ..Results also suggest that the alterations observed in gene expression profiles of Tregs in mCRPC patients may be part of the mechanism of tumor escape from host immune surveillance...
  29. ncbi request reprint Metastasis-associated gene expression profile of liver and subcutaneous lesions derived from mouse pheochromocytoma cells
    Shoichiro Ohta
    Reproductive Biology and Medicine Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 1109, USA
    Mol Carcinog 47:245-51. 2008
    ....
  30. pmc Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closure
    Jacob D Brown
    Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:1462-7. 2009
    ..We also identify misregulation of pax2 in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstream of the Pax2 pathway in directing proper closure of the optic fissure...
  31. ncbi request reprint Serial analysis of gene expression in adrenocortical hyperplasia caused by a germline PRKAR1A mutation
    Anelia Horvath
    Section on Endocrinology and Genetics, Developmental Endocrinology Branch, National Institute of Child Health and Human Development NIH, Building 10, Clinical Research Center, Room I 1330, 10 Center Drive, MSC 1103, Bethesda, MD 20892 1103, USA
    J Clin Endocrinol Metab 91:584-96. 2006
    ..Adrenocortical tumors have been studied at the molecular genetic and cytogenetic levels, but the gene expression profiles of normal and tumor adrenal tissue have not been extensively investigated...
  32. ncbi request reprint A novel function of differentiation revealed by cDNA microarray profiling of p75NTR-regulated gene expression
    Angele Nalbandian
    Department of Cell Biology, Georgetown University Medical Center, Medical Dental Building, Washington, DC 20057 1436, USA
    Differentiation 73:385-96. 2005
    ....
  33. ncbi request reprint Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility
    Zuping He
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, District of Columbia 20057, USA
    Reproduction 132:11-9. 2006
    ..Moreover, issues pertaining to measurement reproducibility are highlighted for the application of microarray data to male infertility...
  34. ncbi request reprint Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice
    Ying Liu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Endocrinol 19:2380-9. 2005
    ....
  35. pmc The Y-encoded TSPY protein: a significant marker potentially plays a role in the pathogenesis of testicular germ cell tumors
    Yunmin Li
    Department of Medicine, VA Medical Center, University of California, San Francisco, CA 94121, USA
    Hum Pathol 38:1470-81. 2007
    ..Our results suggest that TSPY, in combination with other markers, could be an important marker for diagnosis and subclassification of TGCTs and support its role in the pathogenesis of both gonadoblastoma and TGCTs...
  36. ncbi request reprint Variable presentation of precocious puberty associated with the D564G mutation of the LHCGR gene in children with testotoxicosis
    George S Jeha
    Department of Pediatrics, Baylor College of Medicine and Texas Children s Hospital, Houston, Texas 77030, USA
    J Pediatr 149:271-4. 2006
    ..This report underscores the possibility that the effects of the mutant luteinizing hormone/choriogonadotropin receptor on phenotypic expression of FMPP, such as adult final height, are modified by other factors...