K C Chan

Summary

Affiliation: National Cancer Institute
Country: USA

Publications

  1. ncbi request reprint High-speed screening of polymerase chain reaction products by capillary electrophoresis
    K C Chan
    Chemical Synthesis and Analysis Laboratory, SAIC Frederick, National Cancer Institute Frederick Cancer Research and Development Center, Maryland 21702, USA
    Anal Biochem 243:133-9. 1996
  2. ncbi request reprint Analysis of fullerene-based nanomaterial in serum matrix by CE
    King C Chan
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 28:1518-24. 2007
  3. ncbi request reprint The effect of column length, applied voltage, gel type, and concentration on the capillary electrophoresis separation of DNA fragments and polymerase chain reaction products
    H J Issaq
    SAIC Frederik, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 18:1153-8. 1997
  4. ncbi request reprint A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 20:1533-7. 1999
  5. ncbi request reprint Solid-state UV laser-induced fluorescence detection in capillary electrophoresis
    K C Chan
    Analytical Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 21:2062-6. 2000
  6. ncbi request reprint Effect of temperature on the separation of DNA fragments by high-performance liquid chromatography and capillary electrophoresis: a comparative study
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr B Biomed Sci Appl 738:243-8. 2000
  7. ncbi request reprint Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser-induced fluorescence detection
    K C Chan
    SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr A 718:203-10. 1995
  8. ncbi request reprint Multidimensional high performance liquid chromatography--capillary electrophoresis separation of a protein digest: an update
    H J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute at Frederick, MD 21702, USA
    Electrophoresis 22:1133-5. 2001
  9. ncbi request reprint Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cells
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 4:523-31. 2005
  10. ncbi request reprint Quantitative analysis of the low molecular weight serum proteome using 18O stable isotope labeling in a lung tumor xenograft mouse model
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, National Cancer Institute at Frederick, SAIC Frederick, Inc, Frederick, Maryland 21702, USA
    J Am Soc Mass Spectrom 16:1221-30. 2005

Collaborators

Detail Information

Publications32

  1. ncbi request reprint High-speed screening of polymerase chain reaction products by capillary electrophoresis
    K C Chan
    Chemical Synthesis and Analysis Laboratory, SAIC Frederick, National Cancer Institute Frederick Cancer Research and Development Center, Maryland 21702, USA
    Anal Biochem 243:133-9. 1996
    ..This high-speed separation, with minimum postrun data processing, is highly desirable for the high-throughput screening of PCR products using a single-capillary CE system...
  2. ncbi request reprint Analysis of fullerene-based nanomaterial in serum matrix by CE
    King C Chan
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, NCI Frederick, Frederick, MD 21702, USA
    Electrophoresis 28:1518-24. 2007
    ..The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL...
  3. ncbi request reprint The effect of column length, applied voltage, gel type, and concentration on the capillary electrophoresis separation of DNA fragments and polymerase chain reaction products
    H J Issaq
    SAIC Frederik, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 18:1153-8. 1997
    ..The Sigma DNA replaceable gel gave reproducible results when used as received or diluted at 60%. In our hands hydroxyethyl cellulose gave more reproducible results than polyacrylamide gel...
  4. ncbi request reprint A simple two-dimensional high performance liquid chromatography/high performance capillary electrophoresis set-up for the separation of complex mixtures
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 20:1533-7. 1999
    ..This set-up is practical, simple, robust and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of protein digest...
  5. ncbi request reprint Solid-state UV laser-induced fluorescence detection in capillary electrophoresis
    K C Chan
    Analytical Chemistry Laboratory, SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Electrophoresis 21:2062-6. 2000
    ..Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated...
  6. ncbi request reprint Effect of temperature on the separation of DNA fragments by high-performance liquid chromatography and capillary electrophoresis: a comparative study
    H J Issaq
    SAIC Frederick, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr B Biomed Sci Appl 738:243-8. 2000
    ..Also, the optimum HPLC temperature might be different depending on the fragment sizes to be resolved...
  7. ncbi request reprint Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser-induced fluorescence detection
    K C Chan
    SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA
    J Chromatogr A 718:203-10. 1995
    ..Preliminary results show that the MEKC-LIF with direct sample injection is a feasible method for assessing indole profiles in diluted urine and serum...
  8. ncbi request reprint Multidimensional high performance liquid chromatography--capillary electrophoresis separation of a protein digest: an update
    H J Issaq
    Analytical Chemistry Laboratory, SAIC Frederick, National Cancer Institute at Frederick, MD 21702, USA
    Electrophoresis 22:1133-5. 2001
    ..The total experimental time for the HPLC and capillary electrophoretic analyses ist less than 1 h, which ist much faster than using 2-D slab-gel electrophoresis or single-capillary capillary electrophoresis...
  9. ncbi request reprint Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cells
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 4:523-31. 2005
    ..The quantitative profiling of Ib-induced DRMP from Vero cells revealed several proteins with altered expression levels suggesting their possible role in Ib binding/uptake...
  10. ncbi request reprint Quantitative analysis of the low molecular weight serum proteome using 18O stable isotope labeling in a lung tumor xenograft mouse model
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, National Cancer Institute at Frederick, SAIC Frederick, Inc, Frederick, Maryland 21702, USA
    J Am Soc Mass Spectrom 16:1221-30. 2005
    ..The methodology adopted in this work provides the means for future quantitative measurements in comparative animal models of disease and in human disease cohorts...
  11. ncbi request reprint Investigation of the mouse serum proteome
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702, USA
    J Proteome Res 4:1561-8. 2005
    ..The results presented here represent the broadest proteome coverage in mouse serum and provide a foundation from which quantitative comparisons can be made in this important animal model...
  12. pmc Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Proteome Res 5:349-60. 2006
    ..This result confirms previous, targeted studies suggesting that DRMMs isolated using Triton X-100 and Brij-96 differ in their protein content...
  13. ncbi request reprint Identification of the SELDI ProteinChip human serum retentate by microcapillary liquid chromatography-tandem mass spectrometry
    Ming Zhou
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 5:2207-16. 2006
    ..This analysis resulted in the identification of 383 unique proteins in the WCX-2 serum retentate. Among the proteins identified, 50 (13%) are documented clinical biomarkers with 36 of these (72%) identified from multiple peptides...
  14. pmc Mass spectrometry reveals specific and global molecular transformations during viral infection
    Eden P Go
    Department of Molecular Biology and The Center for Mass Spectrometry, The Scripps Research Institute, La Jolla, California 92037, USA
    J Proteome Res 5:2405-16. 2006
    ..Overall, the cellular transformation that occurs upon viral infection is a process involving hundreds of proteins and metabolites, many of which are structurally and functionally uncharacterized...
  15. ncbi request reprint Proteomic analysis of plasma membrane from hypoxia-adapted malignant melanoma
    Luke H Stockwin
    Drug Mechanisms Group and Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    J Proteome Res 5:2996-3007. 2006
    ..Antibody-based analysis of a panel of human melanoma cell lines confirmed that CD13 and SDF-1 were consistently upregulated during hypoxia. This study provides the basis for the discovery of novel hypoxia-induced membrane proteins...
  16. pmc Quantitative proteomic analysis of human breast epithelial cells with differential telomere length
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Biochem Biophys Res Commun 356:942-7. 2007
    ..Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions...
  17. ncbi request reprint Identification of membrane proteins from mammalian cell/tissue using methanol-facilitated solubilization and tryptic digestion coupled with 2D-LC-MS/MS
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    Nat Protoc 1:2784-90. 2006
    ..The entire procedure--beginning with isolated membrane fraction and finishing with MS data acquisition--takes 4-5 d...
  18. ncbi request reprint Multidimensional separation of peptides for effective proteomic analysis
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland, MA 21702, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 817:35-47. 2005
    ..This manuscript provides a review of two- and three-dimensional peptide separation strategies combined with MS for the analysis of complex peptide mixtures...
  19. ncbi request reprint Quantitative proteomic analysis of sokotrasterol sulfate-stimulated primary human endothelial cells
    Aly Karsan
    Department of Medical Biophysics, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada
    Mol Cell Proteomics 4:191-204. 2005
    ..These findings are consistent with a role for sokotrasterol sulfate in endothelial sprouting...
  20. ncbi request reprint An investigation into the human serum "interactome"
    Ming Zhou
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD, USA
    Electrophoresis 25:1289-98. 2004
    ..Among the 210 proteins identified, 73% and 67% were not found in previous studies of the low-molecular-weight or whole-serum proteome, respectively...
  21. ncbi request reprint Global analysis of the cortical neuron proteome
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 3:896-907. 2004
    ..The results presented here provide the broadest proteome coverage for a mammalian cell to date and show that MS-based proteomics has the potential to provide high coverage of the proteins expressed within a cell...
  22. ncbi request reprint Two-dimensional liquid chromatography-capillary zone electrophoresis-sheathless electrospray ionization-mass spectrometry: evaluation for peptide analysis and protein identification
    George M Janini
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 1201, USA
    Electrophoresis 25:1973-80. 2004
    ..Unlike first-dimensional ion-exchange LC fractionation, where there is a large degree of overlap, the CZE-MS results show less than 15% overlap between neighboring RPLC fractions...
  23. ncbi request reprint Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 6:4150-62. 2007
    ..The study also indicates that evaluation of confidence levels for phosphopeptide identification via the reversed sequence database searching strategy might underestimate the false positive rate...
  24. ncbi request reprint Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:862-70. 2004
    ..These peptides resulted in identification of 2563 proteins of which 876 (34%) are classified as membrane proteins...
  25. ncbi request reprint A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland, USA
    J Invest Dermatol 123:691-9. 2004
    ..Furthermore, 121 proteins from cholesterol-rich plasma membrane domains (caveolar and lipid rafts) were identified...
  26. ncbi request reprint Sheathless electrospray ionization interfaces for capillary electrophoresis-mass spectrometric detection advantages and limitations
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 20702, USA
    J Chromatogr A 1053:37-42. 2004
    ..The advantages and limitations of three sheathless designs are discussed and commented upon, these include single-capillary, two-capillary and three-piece designs...
  27. ncbi request reprint Two dimensional mapping of cancer cell extracts by liquid chromatography-capillary electrophoresis with ultraviolet absorbance detection
    Yan He
    Ames Laboratory USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    J Chromatogr A 979:81-9. 2002
    ..In this way, high-resolution analysis of even the minor components in the complicated mixture was possible...
  28. ncbi request reprint Characterization of the low molecular weight human serum proteome
    Radhakrishna S Tirumalai
    SAIC Frederick Inc, Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 2:1096-103. 2003
    ..The large number of proteins identified demonstrates the efficacy of this method for the removal of large abundant proteins and the enrichment of the LMW serum proteome...
  29. ncbi request reprint Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts
    Zhen Xiao
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    J Cell Physiol 210:325-35. 2007
    ..This investigation represents the first systematic proteomic analysis of MVs and provides insights into both the function and origin of these important mineralization-regulating vesicles...
  30. ncbi request reprint Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, Fredrick, MD, USA
    Electrophoresis 25:1342-52. 2004
    ..41-fold increase as determined by Western blotting. These results demonstrate that quantitative proteomics is capable of providing a quantitative view of thousands of proteins in mammalian cells within a defined set of experiments...
  31. pmc Parallel analysis of transcript and translation profiles: identification of metastasis-related signal pathways differentially regulated by drug and genetic modifications
    Haiyan Yang
    Laboratory of Population Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 5:1555-67. 2006
    ....
  32. ncbi request reprint A capillary electrophoretic assay for ribonuclease H activity
    King C Chan
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Center for Cancer Research, NCI at Frederick, Frederick, MD 21702, USA
    Anal Biochem 331:296-302. 2004
    ..The assay was broadly applicable to other RNases H, specifically those from human, Escherichia coli, and HIV-2, although product profiles varied for each enzyme...