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Genomes and Genes | Bradley A CarlsonSummaryAffiliation: National Institutes of Health Country: USA Publications
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Podocyte specific knock out of selenoproteins does not enhance nephropathy in streptozotocin diabetic C57BL/6 miceMarsha N Blauwkamp
Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, MI 48109 5678, USA
BMC Nephrol 9:7. 2008..Since oxidative stress has been implicated in the pathogenesis of diabetic nephropathy, we hypothesized that selenoproteins protect against this complication of diabetes...- Ribosomal frameshifting in response to hypomodified tRNAs in Xenopus oocytesBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Room 6032, Bethesda, MD 20892, USA
Biochem Biophys Res Commun 375:86-90. 2008..Both tRNAAsn+Q and tRNAAsn-Q were used indiscriminately in frameshifting, whether the frameshift site contained the wild-type AAC, or the mutant AAU codon, suggesting that Q base modification status does not influence this process... - Mouse models targeting selenocysteine tRNA expression for elucidating the role of selenoproteins in health and developmentBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Molecules 14:3509-27. 2009.... - Selenoproteins regulate macrophage invasiveness and extracellular matrix-related gene expressionBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
BMC Immunol 10:57. 2009..Incorporation of selenium into the amino acid selenocysteine (Sec), and subsequently into selenoproteins is mediated by Sec tRNA([Ser]Sec)... - Role of selenium-containing proteins in T-cell and macrophage functionBradley A Carlson
Molecular of Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Nutr Soc 69:300-10. 2010..These observations provided novel insights into the role of selenoproteins in the immune function and tissue homeostasis... 
Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machineryXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 280:41568-75. 2005..The data also reveal a role of SECp43 in regulation of selenoprotein expression by affecting the synthesis of Um34 on tRNA([Ser]Sec) and the intracellular location of SLA...- Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cellsRobert Irons
Nutritional Science Research Group, Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, Rockville, MD 20892 7328, USA
Cancer Prev Res (Phila) 3:630-9. 2010..These data suggest tissue specificity in the cancer-protective effects of Sep15 downregulation, which are mediated, at least in part, by influencing the cell cycle... - Biosynthesis of selenocysteine on its tRNA in eukaryotesXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Biol 5:e4. 2007..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins... - Selenoproteins are essential for proper keratinocyte function and skin developmentAniruddha Sengupta
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 5:e12249. 2010..This study links severe selenoprotein deficiency to abnormalities in skin and hair and provides genetic evidence for the role of these proteins in keratinocyte function and cutaneous development... - Specific excision of the selenocysteine tRNA[Ser]Sec (Trsp) gene in mouse liver demonstrates an essential role of selenoproteins in liver functionBradley A Carlson
Section on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland, 20892, USA
J Biol Chem 279:8011-7. 2004..Death appeared to be due to severe hepatocellular degeneration and necrosis with concomitant necrosis of peritoneal and retroperitoneal fat. These studies revealed an essential role of selenoproteins in liver function... - Selective restoration of the selenoprotein population in a mouse hepatocyte selenoproteinless background with different mutant selenocysteine tRNAs lacking Um34Bradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 282:32591-602. 2007..High copy numbers of A34 transgene, but not G37 transgene, were not tolerated in the absence of wild type trsp, further suggesting insertion of Sec in place of Cys in selenoproteins... - A new strategy for assessing selenoprotein function: siRNA knockdown/knock-in targeting the 3'-UTRMin Hyuk Yoo
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Institutes of Health, Bethesda, Maryland 20892, USA
RNA 13:921-9. 2007.... - New developments in selenium biochemistry: selenocysteine biosynthesis in eukaryotes and archaeaXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
Biol Trace Elem Res 119:234-41. 2007..Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that contain selenoproteins... - A functional link between housekeeping selenoproteins and phase II enzymesAniruddha Sengupta
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, CCR, NCI, NIH, Bethesda, MD 20892, USA
Biochem J 413:151-61. 2008..Thus the present study provides evidence for a functional link between housekeeping selenoproteins and phase II enzymes... - Selenophosphate synthetase 2 is essential for selenoprotein biosynthesisXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 404:115-20. 2007..These in vivo studies indicate that SPS2 is essential for generating the selenium donor for selenocysteine biosynthesis in mammals, whereas SPS1 probably has a more specialized, non-essential role in selenoprotein metabolism... - Selenoproteins mediate T cell immunity through an antioxidant mechanismRajeev K Shrimali
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA
J Biol Chem 283:20181-5. 2008..These findings offer novel insights into immune function of selenium and physiological antioxidants... - Selenoprotein expression is essential in endothelial cell development and cardiac muscle functionRajeev K Shrimali
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Neuromuscul Disord 17:135-42. 2007..The data suggest a direct connection between the loss of selenoprotein expression in these cell types and cardiovascular disease... - Targeted insertion of cysteine by decoding UGA codons with mammalian selenocysteine machineryXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:21430-4. 2010..These data reveal a novel Sec machinery-based mechanism for biosynthesis and insertion of Cys into protein at UGA codons and suggest new biological functions for thiophosphate and sulfide in mammals... - Delineating the role of glutathione peroxidase 4 in protecting cells against lipid hydroperoxide damage and in Alzheimer's diseaseMin Hyuk Yoo
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Antioxid Redox Signal 12:819-27. 2010..These data suggest a role of GPx4 in neurodegenerative diseases through its function in removal of lipid hydroperoxides... - Selective rescue of selenoprotein expression in mice lacking a highly specialized methyl group in selenocysteine tRNABradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 280:5542-8. 2005..Overall, this study described a novel mechanism of regulation of protein expression by tRNA modification that is in turn regulated by levels of the trace element, selenium... - Targeting thioredoxin reductase 1 reduction in cancer cells inhibits self-sufficient growth and DNA replicationMin Hyuk Yoo
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 2:e1112. 2007..These observations provide evidence that TR1 is critical for self-sufficiency in growth signals of malignant cells, that TR1 acts largely as a pro-cancer protein and it is indeed a primary target in cancer therapy... - Thioredoxin reductase 1 protects against chemically induced hepatocarcinogenesis via control of cellular redox homeostasisBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Carcinogenesis 33:1806-13. 2012..Overall, this study shows that TR1 protects against chemically induced hepatocarcinogenesis via the control of the cellular redox state, whereas its role in promoting this type of cancer is minimal... - Selenocysteine tRNA identification in the model organisms Dictyostelium discoideum and Tetrahymena thermophilaRajeev K Shrimali
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Biophys Res Commun 329:147-51. 2005..discoideum is 91 nucleotides long making it the longest eukaryotic sequenced to date. Evolutionary analyses of known Sec tRNAs reveal the two forms identified herein are the most divergent eukaryotic Sec tRNAs thus far sequenced... - Protein kinase-regulated expression and immune function of thioredoxin reductase 1 in mouse macrophagesBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Immunol 49:311-6. 2011..These results reveal TR1 as both a regulator and a regulated target in the macrophage gene expression network, and suggest a link between selenium metabolism and immune signaling... - Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expressionXue Ming Xu
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
Nat Protoc 4:1338-48. 2009..The entire knockdown or knock-in procedure can be completed in approximately 3-4 months... - HIF-independent regulation of thioredoxin reductase 1 contributes to the high levels of reactive oxygen species induced by hypoxiaSalvador Naranjo-Suarez
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, Bethesda, Maryland, United States of America
PLoS ONE 7:e30470. 2012..These results indicate that hypoxic TR1 down-regulation is important in maintaining high levels of ROS under hypoxic conditions and that HIF stabilization and activity do not require hypoxic generation of ROS... - Thioredoxin reductase 1 deficiency enhances selenite toxicity in cancer cells via a thioredoxin-independent mechanismRyuta Tobe
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 445:423-30. 2012..The results also suggest that the enhanced selenite toxicity of cancer cells and simultaneous inhibition of TR1 can provide a new avenue for cancer therapy... - Knockout of the 15 kDa selenoprotein protects against chemically-induced aberrant crypt formation in micePetra A Tsuji
Department of Biological Sciences, Towson University, Towson, Maryland, United States of America
PLoS ONE 7:e50574. 2012..Overall, our results demonstrate for the first time, that Sep15 knockout mice are protected against chemically-induced aberrant crypt foci formation and that Sep15 appears to have oncogenic properties in colon carcinogenesis in vivo... - Loss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolismAniruddha Sengupta
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Biophys Res Commun 365:446-52. 2008..These data correlate with reports that selenium deficiency results in increased levels of ApoE, indicating for the first time that housekeeping selenoproteins have a role in regulating lipoprotein biosynthesis and metabolism... - Alteration of thioredoxin reductase 1 levels in elucidating cancer etiologyMin Hyuk Yoo
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
Methods Enzymol 474:255-75. 2010.... - Deficiency in the 15 kDa selenoprotein inhibits human colon cancer cell growthPetra A Tsuji
Cancer Prevention Fellowship Program, National Cancer Institute, Rockville, MD 20892, USA
Nutrients 3:805-17. 2011..The potential mechanism by which human colon cancer cells lacking Sep15 revert their cancer phenotype will need to be explored further... - The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of miceBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, NCI National Cancer Institute, NIH National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 418:61-71. 2009..The results indicate that STAF influences selenoprotein expression by enhancing Trsp synthesis in an organ-specific manner and by controlling Sec tRNA modification in each tissue examined... - Rhesus monkey simian immunodeficiency virus infection as a model for assessing the role of selenium in AIDSXue-Ming Xu
Section on the Molecular Biology of Selenium, Basic Research Laboratory, National Cancer Institute/NIH, Building 37, Room 2D09, Bethesda, MD 20892, USA
J Acquir Immune Defic Syndr 31:453-63. 2002.... - Selenoprotein T deficiency alters cell adhesion and elevates selenoprotein W expression in murine fibroblast cellsAniruddha Sengupta
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Biochem Cell Biol 87:953-61. 2009..Furthermore, we found that the loss of SelT elevates expression of another selenoprotein, selenoprotein W (SepW1). SelT and SepW1 belong to the same protein family, suggesting that SepW1 may functionally compensate for SelT... - Thioredoxin reductase 1 deficiency reverses tumor phenotype and tumorigenicity of lung carcinoma cellsMin Hyuk Yoo
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:13005-8. 2006..These observations provide direct evidence that the reduction of TR1 levels in malignant cells is antitumorigenic and suggest that the enzyme is a prime target for cancer therapy... - Both selenoproteins and low molecular weight selenocompounds reduce colon cancer risk in mice with genetically impaired selenoprotein expressionRobert Irons
Nutritional Science Research Group, National Cancer Institute, Rockville, MD 20852, USA
J Nutr 136:1311-7. 2006..These results are, to our knowledge, the first to provide evidence that both selenoproteins and low molecular weight selenocompounds are important for the cancer-protective effects of selenium... - Selenocysteine incorporation machinery and the role of selenoproteins in development and healthDolph L Hatfield
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Prog Nucleic Acid Res Mol Biol 81:97-142. 2006 - Models for assessing the role of selenoproteins in healthMohamed E Moustafa
Section on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Nutr 133:2494S-2496S. 2003..Both approaches provide important tools for examining the role of selenoproteins in health... - Selenium and selenoprotein deficiencies induce widespread pyogranuloma formation in mice, while high levels of dietary selenium decrease liver tumor size driven by TGFαMohamed E Moustafa
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 8:e57389. 2013..4 and 2.25 versus 0.1 ppm of selenium. Thus, selenium and selenoprotein deficiencies led to widespread pyogranuloma formation, while high selenium levels inhibited the size of TGFα-induced liver tumors... - Selenoproteins reduce susceptibility to DMBA-induced mammary carcinogenesisTamaro S Hudson
Transgenic Oncogenesis and Genomics Section, Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Carcinogenesis 33:1225-30. 2012.... - The Drosophila selenoprotein BthD is required for survival and has a role in salivary gland developmentSo Yeon Kwon
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 23:8495-504. 2003..Duplex RNAi induced from this construct targeted BthD mRNA for destruction and reduced BthD protein levels. We found that loss of BthD compromised salivary gland morphogenesis and reduced animal viability... - Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinaseBradley A Carlson
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 101:12848-53. 2004..The fact that PSTK has been highly conserved in evolution suggests that it plays an important role in selenoprotein biosynthesis and/or regulation... - Chlamydomonas reinhardtii selenocysteine tRNA[Ser]SecMahadev Rao
Section on the Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
RNA 9:923-30. 2003..Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec tRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes... - Selective removal of the selenocysteine tRNA [Ser]Sec gene (Trsp) in mouse mammary epitheliumEaswari Kumaraswamy
Section on Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 23:1477-88. 2003..Thus, the conditional Trsp knockout mouse allows tissue-specific manipulation of Sec tRNA and selenoprotein expression, suggesting that this approach will provide a useful tool for studying the role of selenoproteins in health... - Role of secretoglobin 3A2 in lung developmentReiko Kurotani
Laboratory of Metabolism, Laboratory of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Am J Respir Crit Care Med 178:389-98. 2008..5 during mouse development, which markedly increases by E16.5, the time when lung undergoes dramatic morphologic changes, suggesting that SCGB3A2 may be involved in lung development in addition to a known role in lung inflammation... - Selenoproteins that function in cancer prevention and promotionDolph L Hatfield
Molecular Biology of Selenium Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 6032A Bethesda, MD 20892, USA
Biochim Biophys Acta 1790:1541-5. 2009..In addition, we discuss examples of inhibition of cancer development by selenoprotein deficiency in mouse models. These studies highlight the complex nature of selenium in relation to cancer... - Transfer RNAs that insert selenocysteineBradley A Carlson
Section on Molecular Biology of Selenium, Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Methods Enzymol 347:24-39. 2002 - A recoding element that stimulates decoding of UGA codons by Sec tRNA[Ser]SecMichael T Howard
Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112, USA
RNA 13:912-20. 2007..The variation in recoding elements predicted near UGA-Sec codons implies that these elements may play a differential role in determining the amount of selenoprotein produced by acting as controllers of UGA decoding efficiency...