Heping Cao

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases
    Heping Cao
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Mail Drop F3 04, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 412:106-20. 2003
  2. pmc Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications
    Heping Cao
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 43:13724-38. 2004
  3. pmc Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein
    Heping Cao
    Laboratory of Signal Transduction and Office of Clinical Research, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:21489-99. 2004
  4. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
  5. pmc Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, U S Department of Agriculture ARS, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Biotechnol Prog 24:326-33. 2008
  6. doi request reprint Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, USA
    Obesity (Silver Spring) 16:1208-18. 2008
  7. pmc Phosphorylation of recombinant tristetraprolin in vitro
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA
    Protein J 27:163-9. 2008
  8. pmc Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin
    Heping Cao
    US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Expert Rev Proteomics 4:711-26. 2007
  9. ncbi request reprint Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes
    Heping Cao
    Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705 2350, USA
    Arch Biochem Biophys 459:214-22. 2007
  10. ncbi request reprint Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    J Nutr 138:833-40. 2008

Detail Information

Publications10

  1. pmc Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases
    Heping Cao
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Mail Drop F3 04, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 412:106-20. 2003
    ....
  2. pmc Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications
    Heping Cao
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 43:13724-38. 2004
    ..The results support a model in which each subunit of the TTP tetramer binds to one of the five overlapping UUAUUUAUU sequences of the ARE, resulting in a stable TTP-ARE complex...
  3. pmc Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein
    Heping Cao
    Laboratory of Signal Transduction and Office of Clinical Research, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:21489-99. 2004
    ..TTP appears to be a low abundance, cytosolic protein in unstimulated cells and tissues, but once induced is relatively stable, in contrast to its very labile mRNA...
  4. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
    ....
  5. pmc Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, U S Department of Agriculture ARS, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Biotechnol Prog 24:326-33. 2008
    ..These results demonstrate that ZFP36L1 antiserum is useful in the detection of this protein and that TTP and ZFP36L1 are differentially expressed and regulated at the mRNA and protein levels in mouse adipocytes and macrophages...
  6. doi request reprint Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, USA
    Obesity (Silver Spring) 16:1208-18. 2008
    ..We analyzed the relative abundance of TTP family mRNAs in 3T3-L1 adipocytes compared to RAW264.7 macrophages and investigated insulin effects on the expression of 43 genes in 3T3-L1 adipocytes...
  7. pmc Phosphorylation of recombinant tristetraprolin in vitro
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA
    Protein J 27:163-9. 2008
    ..Autoradiography showed that TTP was phosphorylated in vitro by GSK3b, PKA, PKB, PKC, but not Cdc2, in addition to p42, p38, and JNK. These results demonstrate that TTP is a substrate for a number of protein kinases in vitro...
  8. pmc Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin
    Heping Cao
    US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Expert Rev Proteomics 4:711-26. 2007
    ..This information should provide the molecular basis for future investigation of TTP's regulatory functions in controlling proinflammatory cytokines...
  9. ncbi request reprint Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes
    Heping Cao
    Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705 2350, USA
    Arch Biochem Biophys 459:214-22. 2007
    ..These results suggest that cinnamon exhibits the potential to increase the amount of proteins involved in insulin signaling, glucose transport, and anti-inflammatory/anti-angiogenesis response...
  10. ncbi request reprint Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    J Nutr 138:833-40. 2008
    ..These results suggest that CPE can affect immune responses by regulating anti- and proinflammatory and GLUT gene expression...