Rodrigo T Calado

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Telomere maintenance and human bone marrow failure
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 111:4446-55. 2008
  2. pmc Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:1187-92. 2009
  3. pmc Sex hormones, acting on the TERT gene, increase telomerase activity in human primary hematopoietic cells
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Blood 114:2236-43. 2009
  4. pmc A spectrum of severe familial liver disorders associate with telomerase mutations
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 4:e7926. 2009
  5. doi request reprint Short telomeres result in chromosomal instability in hematopoietic cells and precede malignant evolution in human aplastic anemia
    R T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Leukemia 26:700-7. 2012
  6. pmc Mutations in the SBDS gene in acquired aplastic anemia
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Blood 110:1141-6. 2007
  7. pmc Constitutional telomerase mutations are genetic risk factors for cirrhosis
    Rodrigo T Calado
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Hepatology 53:1600-7. 2011
  8. ncbi request reprint Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia
    Hiroki Yamaguchi
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    N Engl J Med 352:1413-24. 2005
  9. pmc Human telomere disease due to disruption of the CCAAT box of the TERC promoter
    Anna M Aalbers
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 119:3060-3. 2012
  10. ncbi request reprint Defective stromal cell function in a mouse model of infusion-induced bone marrow failure
    Jichun Chen
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Exp Hematol 33:901-8. 2005

Collaborators

Detail Information

Publications35

  1. pmc Telomere maintenance and human bone marrow failure
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 111:4446-55. 2008
    ....
  2. pmc Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:1187-92. 2009
    ..We propose that short and dysfunctional telomeres limit normal stem cell proliferation and predispose for leukemia by selection of stem cells with defective DNA damage responses that are prone to genome instability...
  3. pmc Sex hormones, acting on the TERT gene, increase telomerase activity in human primary hematopoietic cells
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Blood 114:2236-43. 2009
    ..These findings have potential implications for the choice of current androgenic compounds and the development of future agents for clinical use...
  4. pmc A spectrum of severe familial liver disorders associate with telomerase mutations
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 4:e7926. 2009
    ..Here, we sought to determine the spectrum of clinical manifestations associated with telomerase loss-of-function mutations...
  5. doi request reprint Short telomeres result in chromosomal instability in hematopoietic cells and precede malignant evolution in human aplastic anemia
    R T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Leukemia 26:700-7. 2012
    ..These results provide the first clinical direct evidence in humans that short telomeres in hematopoietic cells are dysfunctional, mediate chromosomal instability and predispose to malignant transformation in a human disease...
  6. pmc Mutations in the SBDS gene in acquired aplastic anemia
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Blood 110:1141-6. 2007
    ..We propose that heterozygosity for the 258 + 2 T>C SBDS mutation predisposes to AA by accelerating telomere shortening of leukocytes via a telomerase-independent mechanism...
  7. pmc Constitutional telomerase mutations are genetic risk factors for cirrhosis
    Rodrigo T Calado
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Hepatology 53:1600-7. 2011
    ..7%; P = 0.0011). One additional patient carried a TERC mutation. The mean telomere length of leukocytes in patients with cirrhosis, including six mutant cases, was shorter than in age-matched controls (P = 0.0004)...
  8. ncbi request reprint Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia
    Hiroki Yamaguchi
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    N Engl J Med 352:1413-24. 2005
    ..We investigated whether mutations in genes for other components of telomerase also occur in aplastic anemia...
  9. pmc Human telomere disease due to disruption of the CCAAT box of the TERC promoter
    Anna M Aalbers
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 119:3060-3. 2012
    ..We propose that current mutation-screening strategies should include gene promoter regions for the diagnosis of telomere diseases. This clinical trial was registered at www.clinicaltrials.gov as #NCT00071045...
  10. ncbi request reprint Defective stromal cell function in a mouse model of infusion-induced bone marrow failure
    Jichun Chen
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1202, USA
    Exp Hematol 33:901-8. 2005
    ..To study bone marrow (BM) stromal damage in a mouse model of infusion-induced BM failure...
  11. pmc Defective telomere elongation and hematopoiesis from telomerase-mutant aplastic anemia iPSCs
    Thomas Winkler
    Hematology Branch, National Heart Lung and Blood Institute NHLBI, NIH, Bethesda, Maryland 0892 1202, USA
    J Clin Invest 123:1952-63. 2013
    ..Our data support the necessity of studying multiple clones when using iPSCs to model disease...
  12. pmc Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy
    Tomoiku Takaku
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 116:e41-55. 2010
    ..The technique should be generally adaptable for basic laboratory investigation and for clinical diagnosis of hematologic diseases...
  13. ncbi request reprint Minor antigen h60-mediated aplastic anemia is ameliorated by immunosuppression and the infusion of regulatory T cells
    Jichun Chen
    Hematology Branch, National Heart, Lung, and Blood Institute, NIH Building 10, Clinical Research Center, 10 Center Drive, Bethesda, MD 20892, USA
    J Immunol 178:4159-68. 2007
    ..Immunosuppressive drug treatment or enhancement of regulatory T cell function abrogated this pathophysiology and protected animals from the development of BM failure...
  14. pmc Association of telomere length of peripheral blood leukocytes with hematopoietic relapse, malignant transformation, and survival in severe aplastic anemia
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, 10 Center Dr, Bldg 10 CRC, Room 3 5140, MSC 1202, Bethesda, MD 20892 1202, USA
    JAMA 304:1358-64. 2010
    ..Variations in telomere length have been reported in severe aplastic anemia but their clinical significance is unknown...
  15. pmc Aplastic anemia
    Neal S Young
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1202, USA
    Curr Opin Hematol 15:162-8. 2008
    ....
  16. pmc Telomere length is inherited with resetting of the telomere set-point
    Y Jeffrey Chiang
    Experimental Immunology Branch, National Cancer Institute, Hematology Branch, National Heart, Lung and Blood Institute, and National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:10148-53. 2010
    ..These findings suggest that the set point of telomere lengths of offspring is determined by the telomere lengths of their parents in the presence of normal expression of telomerase...
  17. pmc Interphase Chromosome Flow-FISH
    Keyvan Keyvanfar
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 120:e54-9. 2012
    ..IC Flow-FISH allows for detecting monosomy 7 without requiring bone marrow procurement or the necessity of metaphase spreads, and wider applications to other chromosomal abnormalities are in development...
  18. pmc Interleukin-23 receptor (IL-23R) gene polymorphisms in acquired aplastic anemia
    Tomoiku Takaku
    Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA
    Ann Hematol 88:653-7. 2009
    ..Our results indicate that these three IL-23R SNPs and serum IL-23 level have no apparent impact on susceptibility to AA...
  19. pmc Natural history of pulmonary fibrosis in two subjects with the same telomerase mutation
    Souheil El-Chemaly
    Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Chest 139:1203-9. 2011
    ..In familial pulmonary fibrosis, asymptomatic preclinical alveolar inflammation associated with mutation in TERT and telomerase insufficiency can progress to fibrotic lung disease over 2 to 3 decades...
  20. ncbi request reprint Genetic variation in telomeric repeat binding factors 1 and 2 in aplastic anemia
    Sharon A Savage
    Section of Genomic Variation, Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Exp Hematol 34:664-71. 2006
    ..Abnormal telomere shortening has been observed in a subset of individuals with aplastic anemia (AA). We hypothesized that genetic variation in two genes critical in telomere biology, TERF1 and TERF2, could be a risk factor for AA...
  21. pmc BCL2A1a over-expression in murine hematopoietic stem and progenitor cells decreases apoptosis and results in hematopoietic transformation
    Jean Yves Métais
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e48267. 2012
    ....
  22. ncbi request reprint PTPN22 620W allele is not associated with aplastic anemia
    Solomon A Graf
    Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA
    Am J Hematol 82:291-2. 2007
    ..Aplastic anemia thus joins a list of autoimmune diseases that commonly lack a major humoral disease component and do not associate with the PTPN22 variant...
  23. doi request reprint No impact of lentiviral transduction on hematopoietic stem/progenitor cell telomere length or gene expression in the rhesus macaque model
    Stephanie E Sellers
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Mol Ther 22:52-8. 2014
    ..These studies did not uncover any global long-term perturbation of proliferation, differentiation, or other important functional parameters of transduced HSPCs in the rhesus macaque model. ..
  24. ncbi request reprint Bystander destruction of hematopoietic progenitor and stem cells in a mouse model of infusion-induced bone marrow failure
    Jichun Chen
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10, Rm 7C118, 9000 Rockville Pike, Bethesda, MD 20892 1652, USA
    Blood 104:1671-8. 2004
    ..This effect can be partially abrogated by anti-IFN-gamma antibody...
  25. pmc Current concepts in the pathophysiology and treatment of aplastic anemia
    Neal S Young
    Hematology Branch, National Heart, Lung, and Blood Institute NIH, 10 Center Drive, Bldg 10 CRC, Rm 3E 5140, Bethesda, MD 20892 1202, USA
    Blood 108:2509-19. 2006
    ..Recent results with alternative sources of stem cells and a variety of conditioning regimens to achieve their engraftment have been promising, with survival in small pediatric case series rivaling conventional transplantation results...
  26. ncbi request reprint Telomerase: not just for the elongation of telomeres
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Bioessays 28:109-12. 2006
    ..These findings open new doors for future explorations to understand telomerase function and its interaction with other cell components in the regulation of cell senescence and tumorigenesis...
  27. pmc Immunologic aspects of hypoplastic myelodysplastic syndrome
    Rodrigo T Calado
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Semin Oncol 38:667-72. 2011
    ..However, not all patients respond to immunosuppression, and the identification of relevant biomarkers for an immune mechanism is necessary to identify those patients who may benefit from this treatment modality...
  28. ncbi request reprint Functional characterization of natural telomerase mutations found in patients with hematologic disorders
    Zhong Tao Xin
    Department of Pathology and Laboratory Medicine, Emory University, 105L Whitehead Biomedical Research Bldg, 615 Michael St, Atlanta, GA 30322, USA
    Blood 109:524-32. 2007
    ..Inherited telomerase gene mutations, therefore, operate by various mechanisms to shorten telomere lengths, leading to limited marrow stem cell reserve and renewal capacity in patients with hematologic disorders...
  29. ncbi request reprint Reduced function of the multidrug resistance P-glycoprotein in CD34+ cells of patients with aplastic anaemia
    Rodrigo T Calado
    Centre for Cell Based Therapy, Department of Clinical Medicine, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
    Br J Haematol 118:320-6. 2002
    ..1%, 0-67.7; P = 0.04), but it did not correlate with disease severity. These results indicate that P-gp function is defective in AA CD34+ cells, pointing to a role of P-gp in increased cell susceptibility to xenobiotics in AA...
  30. ncbi request reprint Lack of mutations in the human telomerase RNA component (hTERC) gene in Fanconi's anemia
    Rodrigo T Calado
    Haematologica 89:1012-3. 2004
    ..Only one patient was heterozygous for the G58A polymorphism. No other mutation or deletion was found. We conclude that hTERC gene mutations do not contribute to telomere shortening in FA...
  31. ncbi request reprint Functional characterization of telomerase RNA variants found in patients with hematologic disorders
    Hinh Ly
    Department of Pathology and Laboratory Medicine, Emory University, Whitehead Biomedical Research Bldg, 615 Michael St, Rm 175, Atlanta, GA 30322, USA
    Blood 105:2332-9. 2005
    ..Therefore, loss of telomerase activity and of telomere maintenance resulting from inherited hTERC mutations may limit marrow stem cell renewal and predispose some patients to bone marrow failure...
  32. ncbi request reprint Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay
    Luiz M da Fonseca
    Department of Clinical Analysis, State University of São Paulo School of Pharmaceutical Sciences, Araraquara, Brazil
    Hematol J 4:26-30. 2003
    ..In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories...
  33. ncbi request reprint Aplastic anaemia and telomerase RNA mutations
    Rodrigo T Calado
    Lancet 360:1608. 2002
  34. ncbi request reprint Influence of functional MDR1 gene polymorphisms on P-glycoprotein activity in CD34+ hematopoietic stem cells
    Rodrigo T Calado
    Center for Cell Based Therapy, Department of Clinical Medicine, University of São Paulo at Ribeirão Preto Medical School, Av Bandeirantes 3900, Ribeirão Preto 14049 900 SP Brazil
    Haematologica 87:564-8. 2002
    ..We investigated whether T-129C, G26677T and C3435T polymorphisms influence P-glycoprotein function in stem cells...
  35. ncbi request reprint MDR1 gene C3435T polymorphism and the risk of acquired aplastic anaemia
    Rodrigo T Calado
    Br J Haematol 117:769. 2002