Research Topics
| J M ButlerSummaryAffiliation: National Institute of Standards and Technology Country: USA Publications
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Detail Information
Publications
The development of reduced size STR amplicons as tools for analysis of degraded DNAJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 48:1054-64. 2003..These miniplex sets should prove valuable in the analysis of samples where allele dropout and reduced sensitivity of larger STR alleles occurs...- Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysisJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899 8311, USA
Electrophoresis 25:1397-412. 2004..Requirements for accurate typing of STR alleles are considered in the context of what future analysis platforms will need to increase sample throughput and ease of use... - Duplication of DYS19 flanking regions in other parts of the Y chromosomeJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Int J Legal Med 118:178-83. 2004..The discovery of this duplicated region of the Y chromosome shows the value of newly available human genome sequence information for assay design and the importance of using sequence queries and alignments in the primer design process... - Genetics and genomics of core short tandem repeat loci used in human identity testingJohn M Butler
National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 51:253-65. 2006..These core STR loci, which form the basis for DNA databases worldwide, will continue to play an important role in forensic science for many years to come... - Allele frequencies for 27 Y-STR loci with U.S. Caucasian, African American, and Hispanic samplesJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int 156:250-60. 2006..Allele frequencies for each locus are reported along with nomenclature based on sequence analysis... - Allele frequencies for 15 autosomal STR loci on U.S. Caucasian, African American, and Hispanic populationsJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA
J Forensic Sci 48:908-11. 2003 - Short tandem repeat typing technologies used in human identity testingJohn M Butler
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Biotechniques 43:ii-v. 2007..This article offers a brief perspective on the technologies and issues involved in STR typing... - U.S. population data for the multi-copy Y-STR locus DYS464John M Butler
National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA
J Forensic Sci 50:975-7. 2005 - Chromosomal duplications along the Y-chromosome and their potential impact on Y-STR interpretationJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:853-9. 2005..In addition, duplicated loci typically possess alleles that differ by only a single repeat unit and possess similar peak heights... - Capillary electrophoresis as a tool for optimization of multiplex PCR reactionsJ M Butler
National Institute of Standards and Technology, Biotechnology Division, Gaithersburg, MD 20899, USA
Fresenius J Anal Chem 369:200-5. 2001..The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process... - Quality control of PCR primers used in multiplex STR amplification reactionsJ M Butler
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Forensic Sci Int 119:87-96. 2001..We present here the use of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) methods for characterization of commercially available STR kits... - Locus-specific brackets for reliable typing of Y-chromosome short tandem repeat markersJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Electrophoresis 26:2583-90. 2005..Complete agreement was observed with 162 samples studied at 5 Y-chromosome loci... - A novel multiplex for simultaneous amplification of 20 Y chromosome STR markersJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Forensic Sci Int 129:10-24. 2002..Haplotype comparisons between the 20plex and a commercially available kit found excellent agreement across the 76 samples in the Y chromosome consortium panel... - High-throughput Y-STR typing of U.S. populations with 27 regions of the Y chromosome using two multiplex PCR assaysRichard Schoske
Biotechnology Division, National Institute of Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Forensic Sci Int 139:107-21. 2004..Samples containing the most common type in the European database could be well resolved with additional markers beyond the minimal haplotype loci... - Genotyping of two mutations in the HFE gene using single-base extension and high-performance liquid chromatographyJ M Devaney
Transgenomic Inc, Gaithersburg, Maryland 20878, USA
Anal Chem 73:620-4. 2001..This assay allows concurrent genotyping of the C282Y and H63D mutations in 11 min and is 100% concordant with current testing methods for both of these mutations... 
The impact of additional Y-STR loci on resolving common haplotypes and closely related individualsA E Decker
National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899, United States
Forensic Sci Int Genet 1:215-7. 2007..S. population groups. Additional Y-STRs can also increase the power of discrimination between closely related male individuals, which is important not only in forensics but also in the paternity and genetic genealogy communities...
Thermodynamic comparison of PNA/DNA and DNA/DNA hybridization reactions at ambient temperatureF P Schwarz
Center for Advanced Research in Biotechnology National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, USA
Nucleic Acids Res 27:4792-800. 1999....- Results from the NIST 2004 DNA Quantitation StudyMargaret C Kline
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:570-8. 2005..Information from this interlaboratory study is guiding development of a future NIST Standard Reference Material for Human DNA Quantitation, SRM 2372... - Multiplex_QA: an exploratory quality assessment tool for multiplexed electrophoretic assaysDavid L Duewer
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
Electrophoresis 27:3735-46. 2006.... - Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kitsCarolyn R Hill
National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
J Forensic Sci 52:870-3. 2007..DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift... - Production and certification of NIST Standard Reference Material 2372 Human DNA Quantitation StandardMargaret C Kline
Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8311, Gaithersburg, MD 20899 8311, USA
Anal Bioanal Chem 394:1183-92. 2009.... - Characterization of 26 miniSTR loci for improved analysis of degraded DNA samplesCarolyn R Hill
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 53:73-80. 2008.... - A 26plex autosomal STR assay to aid human identity testing*Carolyn R Hill
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 54:1008-15. 2009..The 26plex has the potential to benefit the forensic community for reference sample testing and complex relationship evaluation... - Analysis of mutations in father-son pairs with 17 Y-STR lociAmy E Decker
Biochemical Science Division, National Institute of Sciences and Technology, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int Genet 2:e31-5. 2008..Furthermore, two different sample pairs were found to have two mutations. An African American sample pair had a mutation at DYS458 and a second at DYS635 and an Asian sample pair had mutations at DYS439 and Y-GATA-H4... - Demonstration of rapid multiplex PCR amplification involving 16 genetic lociPeter M Vallone
National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int Genet 3:42-5. 2008..84... - Characterization of new miniSTR loci to aid analysis of degraded DNAMichael D Coble
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 50:43-53. 2005..The utility of these new loci were confirmed in comparing the success of the miniSTR assays for typing degraded bone samples while partial profiles were observed with the majority of the samples using a commercial STR kit... - Mitochondrial DNA typing screens with control region and coding region SNPsMargaret C Kline
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
J Forensic Sci 50:377-85. 2005..These types of screening assays should enable more rapid evaluation of forensic casework samples such that only samples not excluded would be subjected to further characterization through full HVI/HVII mtDNA sequence analysis... - Allele frequencies for 70 autosomal SNP loci with U.S. Caucasian, African-American, and Hispanic samplesPeter M Vallone
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
Forensic Sci Int 149:279-86. 2005..The final pair of unrelated samples could be resolved with the combination of 4 STR loci: D2S1338, D18S51, FGA, and VWA... - A multiplex allele-specific primer extension assay for forensically informative SNPs distributed throughout the mitochondrial genomePeter M Vallone
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Int J Legal Med 118:147-57. 2004..We demonstrate that the assay performs well for heteroplasmy and mixture detection, and for typical mtDNA casework samples with highly degraded DNA... - Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) lociRichard Schoske
Department of Chemistry, American University, 4400 Massachusetts Ave NW, Washington DC 20016, USA
Anal Bioanal Chem 375:333-43. 2003..As a model system to illustrate this multiplex design protocol, a 10-loci multiplex (10plex) Y chromosome short tandem repeat (STR) assay is used... - AutoDimer: a screening tool for primer-dimer and hairpin structuresPeter M Vallone
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Biotechniques 37:226-31. 2004..Sodium counterion and total stand concentrations can be adjusted for the Tm and deltaG calculations. The predicted interactions are saved in a text file for further evaluation... - A strategy for examining complex mixtures of deoxyoligonucleotides using ion-pair-reverse-phase high-performance liquid chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and informaticsPeter M Vallone
Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
Anal Biochem 304:257-65. 2002..IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple computational algorithm provide an integrated strategy for determining the sequence of short nucleic acid oligomers... - Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival mediaMargaret C Kline
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Anal Chem 74:1863-9. 2002..All of the evaluated media adequately "bank" forensically useful DNA in well-dried whole blood stains for at least 19 months at ambient temperature... - NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensityMargaret C Kline
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
Anal Chem 75:2463-9. 2003..However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems... - NIST mixed stain study 3: signal intensity balance in commercial short tandem repeat multiplexesDavid L Duewer
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8394, USA
Anal Chem 76:6928-34. 2004.... - Y-SNP typing of U.S. African American and Caucasian samples using allele-specific hybridization and primer extensionPeter M Vallone
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
J Forensic Sci 49:723-32. 2004..A total of 18 unique haplogroups out of a possible 45 were observed in a group of 229 U.S. African American and Caucasian males with the majority of samples being assigned into 2 of the 18 haplogroups... - Constructing STR multiplex assaysJohn M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
Methods Mol Biol 297:53-66. 2005..Primer design parameters and Internet-accessible resources are discussed, as are solutions to problems with residual dye artifacts that result from impure primers... - Rapid mitochondrial DNA typing using restriction enzyme digestion of polymerase chain reaction amplicons followed by capillary electrophoresis separation with laser-induced fluorescence detectionJ M Butler
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
Electrophoresis 19:119-24. 1998..In addition, the presence of a T-->C transition at position 16189, which gives rise to the so-called "C-stretch" in HV1, may be predicted from the presence of nonspecific PCR products in the CE results... - Short tandem repeat analysis for human identity testingJohn M Butler
National Institute of Standards and Technology, Gaithersburg, MD, USA
Curr Protoc Hum Genet . 2004..Finally, future technologies and DNA marker systems are briefly explored relative to the state-of-the-science today... - Forensic value of 14 novel STRs on the human Y chromosomeAlan J Redd
Division of Biotechnology, University of Arizona, Biosciences West room 239, Tucson, AZ 85721, USA
Forensic Sci Int 130:97-111. 2002..Furthermore, these novel Y-STRs greatly improved the resolution of paternal lineages, above the level obtained with commonly used Y-STRs, in the European-American population... - A study on the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer setsDenise T Chung
Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
J Forensic Sci 49:733-40. 2004..Overall, our data confirm that our redesigned primers can increase the probability of obtaining a usable profile in situations where standard kits fail... - Letter to the editor--nomenclature and allele repeat structure update for the Y-STR locus GATA H4Julio J Mulero
J Forensic Sci 51:694. 2006 - Concordance study between Miniplex assays and a commercial STR typing kitJiri Drabek
Ohio University, Department of Chemistry and Biochemistry, Athens, OH 45701, USA
J Forensic Sci 49:859-60. 2004 - Epidemiology. DNA identifications after the 9/11 World Trade Center attackLeslie G Biesecker
Science 310:1122-3. 2005.... - An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samplesMarilyn A Menotti-Raymond
Laboratory of Genomic Diversity, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USA
J Forensic Sci 50:1061-70. 2005..31E-08. A multiplex protocol, designed for the co-amplification of the 11 loci and a gender-identifying locus, is species specific and robust, generating a product profile with as little as 0.125 nanograms of genomic DNA... - Toward increased utility of mtDNA in forensic identificationsRebecca S Just
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, AFIP Annex, 1413 Research Blvd, Rockville, MD 20850, USA
Forensic Sci Int 146:S147-9. 2004..A large-scale databasing effort targeting several populations underrepresented in current mtDNA databases is underway... - Developmental validation of reduced-size STR Miniplex primer setsKerry L Opel
International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA
J Forensic Sci 52:1263-71. 2007..Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA... - 17 beta-Oestradiol attenuates nucleotide excision repairMark D Evans
Oxidative Stress Group, Department of Clinical Biochemistry, P O Box 65, Robert Kilpatrick Clinical Sciences Building, University of Leicester, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester LE2 7LX, UK
FEBS Lett 535:153-8. 2003..In contrast, treatment with 17 alpha-oestradiol had no effect. Mechanisms are proposed for this effect of E2, which may contribute to its carcinogenic potential...