Research Topics
Species | Ursula J BuchholzSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Chimeric recombinant human metapneumoviruses with the nucleoprotein or phosphoprotein open reading frame replaced by that of avian metapneumovirus exhibit improved growth in vitro and attenuation in vivoQuynh N Pham
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 8007, USA
J Virol 79:15114-22. 2005..Both chimeras were comparable to wild-type HMPV in immunogenicity and protective efficacy. Thus, the P chimera is a promising live HMPV vaccine candidate that paradoxically combines improved growth in vitro with attenuation in vivo...- Live vaccines for human metapneumovirus designed by reverse geneticsUrsula J Buchholz
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 50, Room 6505, 50 South Dr MSC 8007, Bethesda, MD 20892 8007, USA
Expert Rev Vaccines 5:695-706. 2006..Additional modifications to provide improved vaccines will also be discussed... - Deletion of nonstructural proteins NS1 and NS2 from pneumonia virus of mice attenuates viral replication and reduces pulmonary cytokine expression and diseaseUrsula J Buchholz
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
J Virol 83:1969-80. 2009..In contrast, in the absence of NS2, there was an early, transient innate response involving moderate levels of IFN, IL-6, and CXCL10 that restricted virus replication and prevented disease... - Deletion of M2 gene open reading frames 1 and 2 of human metapneumovirus: effects on RNA synthesis, attenuation, and immunogenicityUrsula J Buchholz
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8007, USA
J Virol 79:6588-97. 2005..The HMPV rdeltaM2-2 virus is a promising and highly attenuated HMPV vaccine candidate... - Contributions of the structural proteins of severe acute respiratory syndrome coronavirus to protective immunityUrsula J Buchholz
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8007, USA
Proc Natl Acad Sci U S A 101:9804-9. 2004.... - Infection of nonhuman primates with recombinant human metapneumovirus lacking the SH, G, or M2-2 protein categorizes each as a nonessential accessory protein and identifies vaccine candidatesStephane Biacchesi
National Institutes of Health, NIAID, Laboratory of Infectious Diseases, Bethesda, MD 20892 8007, USA
J Virol 79:12608-13. 2005..The deltaG and deltaM2-2 viruses are promising vaccine candidates that are based on independent mechanisms of attenuation and are appropriate for clinical evaluation... - Mucosal immunisation of African green monkeys (Cercopithecus aethiops) with an attenuated parainfluenza virus expressing the SARS coronavirus spike protein for the prevention of SARSAlexander Bukreyev
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Lancet 363:2122-7. 2004..We have developed an experimental SARS vaccine for direct immunisation of the respiratory tract, the major site of SARS- coronavirus transmission and disease... - Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidateStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892 8007, USA
J Virol 78:12877-87. 2004..This indicates that SH is completely dispensable in vivo and that its deletion does not confer an attenuating effect, at least in this rodent model... - Individual contributions of the human metapneumovirus F, G, and SH surface glycoproteins to the induction of neutralizing antibodies and protective immunityMario H Skiadopoulos
Respiratory Viruses Section, Laboratory of Infectious Diseases, NIAID, NIH, DHHS, Bethesda, MD 20892 8007, USA
Virology 345:492-501. 2006..Also, although the SH protein of HMPV is a virion protein that is much larger than its counterparts in previously studied paramyxoviruses, it does not appear to be a significant neutralization or protective antigen... - Infection and maturation of monocyte-derived human dendritic cells by human respiratory syncytial virus, human metapneumovirus, and human parainfluenza virus type 3Cyril Le Nouen
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Virology 385:169-82. 2009..Maturation by these viruses was anti-apoptotic. Inefficient infection of IDC and sub-optimal maturation might result in reduced immune responses, but these effects would be common to all three viruses rather than specific to HRSV... - Rapid human metapneumovirus microneutralization assay based on green fluorescent protein expressionStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Building 50, Room 6505, 50 South Drive, MSC 8007 Bethesda, MD 20892 8007, USA
J Virol Methods 128:192-7. 2005..This assay also permits automation and up-scaling, making it suitable for broad HMPV seroepidemiology studies and experiments that require large scale serology, such as vaccine studies... - Effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on CD4+ T cell activation by dendritic cellsCyril Le Nouen
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
PLoS ONE 5:e15017. 2010..Thus, the results do not support the common model in which viral suppression of CD4+ T cell activation and proliferation by HRSV, HMPV, and HPIV3 is a major factor in the difference in re-infectability compared to IAV... - Modification of the trypsin-dependent cleavage activation site of the human metapneumovirus fusion protein to be trypsin independent does not increase replication or spread in rodents or nonhuman primatesStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 8007, USA
J Virol 80:5798-806. 2006..These results suggest that cleavage activation is not a major determinant of HMPV virulence... - Low CCR7-mediated migration of human monocyte derived dendritic cells in response to human respiratory syncytial virus and human metapneumovirusCyril Le Nouen
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Pathog 7:e1002105. 2011..We propose that inefficient migration of HRSV- and HMPV-stimulated DC to lymphatic tissue contributes to reduced adaptive responses to these viruses... - Respiratory syncytial virus interferon antagonist NS1 protein suppresses and skews the human T lymphocyte responseShirin Munir
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Pathog 7:e1001336. 2011.... - Nonstructural proteins 1 and 2 of respiratory syncytial virus suppress maturation of human dendritic cellsShirin Munir
Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 8007, USA
J Virol 82:8780-96. 2008..The observed suppression of DC maturation may result in decreased antigen presentation and T-lymphocyte activation, leading to incomplete and/or weak immune responses that might contribute to RSV reinfection... - The two major human metapneumovirus genetic lineages are highly related antigenically, and the fusion (F) protein is a major contributor to this antigenic relatednessMario H Skiadopoulos
Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6511, 50 South Dr, MSC 8007, Bethesda, MD 20892 8007, USA
J Virol 78:6927-37. 2004.... - Frequent frameshift and point mutations in the SH gene of human metapneumovirus passaged in vitroStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892 8007, USA
J Virol 81:6057-67. 2007..Adaptation involving the functional loss of a gene is unusual for an RNA virus... - Recovery of human metapneumovirus from cDNA: optimization of growth in vitro and expression of additional genesStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 8007, USA
Virology 321:247-59. 2004.... - Codon stabilization analysis of the "248" temperature sensitive mutation for increased phenotypic stability of respiratory syncytial virus vaccine candidatesCindy Luongo
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 8007, USA
Vaccine 27:5667-76. 2009.... - Evaluation of pneumonia virus of mice as a possible human pathogenLinda G Brock
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
J Virol 86:5829-43. 2012..The absence of PVM-specific antibodies and restriction in nonhuman primates makes PVM unlikely to be a human pathogen... - Genetic diversity between human metapneumovirus subgroupsStephane Biacchesi
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8007, USA
Virology 315:1-9. 2003..It is reasonable to anticipate that the two genetic subgroups of HMPV represent antigenic subgroups approximately comparable to those of HRSV... - The open reading frame 3a protein of severe acute respiratory syndrome-associated coronavirus promotes membrane rearrangement and cell deathEric C Freundt
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 84:1097-109. 2010..These results establish an important role for ORF 3a in SARS-CoV-induced cell death, Golgi fragmentation, and the accumulation of intracellular vesicles... - Increased genetic and phenotypic stability of a promising live-attenuated respiratory syncytial virus vaccine candidate by reverse geneticsCindy Luongo
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
J Virol 86:10792-804. 2012.... - Identification of a novel virulence factor in recombinant pneumonia virus of miceChristine D Krempl
Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany
J Virol 81:9490-501. 2007..In addition to its intrinsic interest, a recombinant virus that replicates with wild-type-like efficiency but does not cause disease defines optimal properties for vaccine development... - Recovery of avian metapneumovirus subgroup C from cDNA: cross-recognition of avian and human metapneumovirus support proteinsDhanasekaran Govindarajan
Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA
J Virol 80:5790-7. 2006..These results indicate a close functional relationship between AMPV/CO and HMPV... - Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSVUlrike Schmidt
Institute of Molecular Biology, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany
J Virol 76:12355-9. 2002..Neutralizing antibodies were induced by rBRSV and rBRSVDeltaG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV... - Mapping and characterization of the primary and anamnestic H-2(d)-restricted cytotoxic T-lymphocyte response in mice against human metapneumovirusGuillermina A Melendi
Fundacion INFANT, Buenos Aires, Argentina
J Virol 81:11461-7. 2007..An understanding of the CTL response against hMPV is important for developing preventive and therapeutic strategies against the virus... - A novel protein expression strategy using recombinant bovine respiratory syncytial virus (BRSV): modifications of the peptide sequence between the two furin cleavage sites of the BRSV fusion protein yield secreted proteins, but affect processing and functPatricia König
Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, 17493 Greifswald Insel Riems, Germany
J Gen Virol 85:1815-24. 2004....